Article
Food Science & Technology
Jewel Ann Joseph, Simen Akkermans, Jan F. M. Van Impe
Summary: The sweet protein thaumatin is a promising sugar replacer, and its large-scale extraction from natural plants can be limited in terms of raw material availability and production costs. This study evaluated the impact of pH and temperature on the production of thaumatin II protein using Pichia pastoris, and found that a pH of 6.0 and a temperature of 30 degrees C resulted in the highest protein levels.
Article
Biotechnology & Applied Microbiology
Fidan Erden-Karaoglan, Mert Karaoglan
Summary: This study investigated the applicability of heterologous yeast promoters in Pichia pastoris, and found inducible and constitutive regulation modes as well as the optimal carbon sources for these promoters. The study also determined the DNA region with the highest activity for all promoters through promoter deletion analysis.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zuzana Rosenbergova, Kristina Kantorova, Martin Simkovic, Albert Breier, Martin Rebros
Summary: The study successfully upscaled the production of Myrosinase to a 3 L laboratory fermenter, optimizing fermentation conditions resulted in a threefold increase in Myrosinase productivity and 1.5-fold increase in dry cell weight.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Microbiology
Yingjie Pan, Jiao Yang, Jianping Wu, Lirong Yang, Hao Fang
Summary: This article summarizes the most recent advances of Pichia pastoris as an efficient platform for recombinant protein production in the past 5 years, including innovative genetic tools and methodologies for manipulating gene expression systems, novel pathways in metabolic engineering, and the application of high-throughput screening coupled with mathematical models.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zuzana Rosenbergova, Zuzana Hegyi, Miroslav Ferko, Natalia Andelova, Martin Rebros
Summary: The deletion of a native signal sequence in plants significantly increased the production of myrosinase in Pichia pastoris, leading to changes in enzyme properties. These findings could provide insights for the expression of other plant enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Cristina Bustos, Johan Quezada, Rhonda Veas, Claudia Altamirano, Stephanie Braun-Galleani, Patrick Fickers, Julio Berrios
Summary: Komagataella phaffii has become an important microorganism for recombinant protein production, particularly in industrial settings. Significant progress has been made in improving recombinant protein productivity and reducing production costs. Cell engineering approaches, including metabolic engineering, transcription factor modulation, and manipulation of protein folding and secretion pathways, have shown promising results. However, more research is needed in optimizing cultivation parameters in larger-scale bioreactors.
Article
Biochemistry & Molecular Biology
Maria Carmela Bonaccorsi di Patti, Antimo Cutone, Zuzana Pakanova, Marek Nemcovic, Peter Barath, Giovanni Musci
Summary: Ceruloplasmin is crucial for iron metabolism in vertebrates, and the production of high-quality recombinant human ceruloplasmin represents a significant advancement. Further improvement in productivity and glycoengineering is necessary for potential therapeutic use in aceruloplasminemia patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Edgar Velastegui, Johan Quezada, Karlo Guerrero, Claudia Altamirano, Juan Andres Martinez, Julio Berrios, Patrick Fickers
Summary: Culture medium heterogeneity in industrial bioreactors leads to concentration gradients due to the loss of mixing efficiency. This study investigated the cell response to transient perturbations of high methanol concentration combined with hypoxia using a scale-down system. The results showed that stressful culture conditions affected cell metabolism, biomass formation, and cell flocculation. Surprisingly, these conditions actually benefited recombinant protein productivity by increasing the expression levels of genes and eGFP.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Immunology
Nicholas J. MacDonald, Kavita Singh, Karine Reiter, Nguyen Vu, Richard Shimp, Apostolos G. Gittis, Beth Chen, Martin Burkhardt, Baoshan Zhang, Zhixiong Wang, Raul Herrera, Mackenzie Moler, Duck-Yeon Lee, Sachy Orr-Gonzalez, Jessica Herrod, Lynn E. Lambert, Kelly M. Rausch, Olga Muratova, David S. Jones, Yimin Wu, Albert J. Jin, David N. Garboczi, Patrick E. Duffy, David L. Narum
Summary: The development of a malaria vaccine that can block transmission of the parasite from mosquitoes to humans is crucial for malaria elimination efforts. The use of Pfs25, a protein on the surface of the parasite, is being investigated as a transmission-blocking vaccine. Two recombinant forms of Pfs25, Pfs25H and Pfs25M, have been studied for their structural and immunological differences.
Article
Biotechnology & Applied Microbiology
Yide Huang, Ting Lin, Lingfang Lu, Fan Cai, Jie Lin, Yu'e Jiang, Yao Lin
Summary: A new codon pair optimization algorithm was introduced in this study, showing that codon optimization based on codon pair bias is more effective in enhancing protein expression levels in Pichia pastoris.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Moo Sun Hong, M. Lourdes Velez-Suberbie, Andrew J. Maloney, Andrew Biedermann, Kerry R. Love, J. Christopher Love, Tarit K. Mukhopadhyay, Richard D. Braatz
Summary: This article constructs a macroscopic bioreactor model for Pichia pastoris to describe cellular metabolism and optimize bioreactor operation. The model predictions accurately describe experimental data and can be used to improve chemically defined medium.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Xingxing Liang, Hong Jiang, Xiandong Si, Qi Xin, Di Meng, Peng Chen, Xiangzhao Mao
Summary: The study successfully increased the expression level of plectasin in recombinant Pichia pastoris by constructing a four-copy cassette of plectasin via 2A peptide assembly. This strategy based on 2A peptide assembly can enhance the expression of plectasin and further expand its application prospect.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mert Karaoglan
Summary: Extracellular protein production is crucial for downstream processes in recombinant protein production. Signal peptide processing plays a key role in protein secretion, and different yeast-derived secretion sequences were tested in Pichia pastoris to improve protein production. Among the tested sequences, H. polymorpha protein internal repeats (HpPIR) and K. lactis alpha-mating factor (Kl-MF) showed the highest yields for xylanase and amylase, respectively. These sequences can be considered as alternatives for protein production in various expression systems.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Richard J. Zahrl, Roland Prielhofer, Jonas Burgard, Diethard Mattanovich, Brigitte Gasser
Summary: Yeasts, such as Pichia pastoris, are a suitable expression system for recombinant proteins, including antibody fragments. Improvements in secretion capacity can be achieved by overexpression of the Msn4 transcription factor or synthetic versions, leading to significant increases in recombinant protein production.
Article
Biotechnology & Applied Microbiology
Lin Cui, Huoqing Huang, Honglian Zhang, Xiaolu Wang, Xing Qin, Tao Tu, Jie Zhang, Xiaoyun Su, Huimin Yu, Yingguo Bai, Huiying Luo, Bin Yao, Yuan Wang
Summary: A novel fusion protein expression strategy was proposed to increase the expression level of hen egg white lysozyme (HEWL). The results showed that the fusion protein expressed using this strategy had higher expression level and activity compared to HEWL alone.
Editorial Material
Biotechnology & Applied Microbiology
Sabrina E. Vinzon, Frank Roesl
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2015)
Article
Biochemical Research Methods
Maxim E. Darvin, Gisela Thiede, Saul Mujica Ascencio, Sabine Schanzer, Heike Richter, Sabrina E. Vinzon, Daniel Hasche, Frank Roesl, Roberto May, Yohan Hazot, Dov Tamarkin, Juergen Lademann
JOURNAL OF BIOMEDICAL OPTICS
(2016)
Article
Biotechnology & Applied Microbiology
Melanie Salvermoser, Sasithorn Chotewutmontri, Ilona Braspenning-Wesch, Daniel Hasche, Frank Roesl, Sabrina E. Vinzon
JOURNAL OF GENERAL VIROLOGY
(2016)
Article
Microbiology
Daniel Hasche, Sonja Stephan, Ilona Braspenning-Wesch, Julita Mikulec, Martina Niebler, Hermann-Josef Groene, Christa Flechtenmacher, Baki Akguel, Frank Roesl, Sabrina E. Vinzon
Review
Microbiology
Daniel Hasche, Sabrina E. Vinzon, Frank Roesl
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Multidisciplinary Sciences
Sabrina E. Vinzon, Cristina Marino-Buslje, Elena Rivera, Mirtha Biscoglio de Jimenez Bonino
Article
Multidisciplinary Sciences
Daniel Hasche, Sonja Stephan, Larissa Savelyeva, Frank Westermann, Frank Roesl, Sabrina E. Vinzon
Article
Pharmacology & Pharmacy
Jose Christian Dokmetjian, Sergio del Canto, Sabrina Vinzon, Mirtha Biscoglio de Jimenez Bonino
Article
Microbiology
Sabrina E. Vinzon, Ilona Braspenning-Wesch, Martin Mueller, Edward K. Geissler, Ingo Nindl, Hermann-Josef Groene, Kai Schaefer, Frank Roesl
Article
Biology
Yingying Fu, Rui Cao, Miriam Schaefer, Sonja Stephan, Ilona Braspenning-Wesch, Laura Schmitt, Ralf Bischoff, Martin Muller, Kai Schafer, Sabrina E. Vinzon, Frank Rosl, Daniel Hasche
Article
Immunology
M. Veronica Lopez, Sabrina E. Vinzon, Eduardo G. A. Cafferata, Felipe J. Nunez, Ariadna Soto, Maximiliano Sanchez-Lamas, M. Jimena Afonso, Diana Aguilar-Cortes, Gregorio D. Rios, Juliana T. Maricato, Carla T. Braconi, Vanessa B. Silveira, Tatiane M. Andrad, Tatiana C. S. Bonetti, Luiz M. Ramos Janini, Manoel J. B. C. Girao, Andrea S. Llera, Karina A. Gomez, Hugo H. Ortega, Paula M. Berguer, Osvaldo L. Podhajcer
Summary: A novel vaccine based on a chimeric human adenovirus 5 vector was developed, which induced potent humoral and T-cell immunity lasting at least 5 months by enhancing Spike expression, infection of muscle and dendritic cells, and utilizing stabilized Spike in a prefusion conformation. Vaccinated mice showed neutralizing antibodies against various Spike variants and maintained stable immunity for over 5 months, suggesting potential for clinical trials.
Article
Immunology
Sabrina E. Vinzon, Maria V. Lopez, Eduardo G. A. Cafferata, Ariadna S. Soto, Paula M. Berguer, Luciana Vazquez, Leonora Nusblat, Andrea V. Pontoriero, Eduardo M. Belotti, Natalia R. Salvetti, Diego L. Viale, Ariel E. Vilardo, Martin M. Avaro, Estefania Benedetti, Mara L. Russo, Maria E. Dattero, Mauricio Carobene, Maximiliano Sanchez-Lamas, Jimena Afonso, Mauro Heitrich, Alejandro E. Cristofalo, Lisandro H. Otero, Elsa G. Baumeister, Hugo H. Ortega, Alexis Edelstein, Osvaldo L. Podhajcer
Summary: The study demonstrates the cross-protective and cross-neutralizing capacity of vaccines expressing variant-matched Spike protein in rodent models, with varying levels of protection observed against different variants. The use of a booster with a distant variant-based vaccine enhances neutralizing capacity and provides broader protection against SARS-CoV-2 lineages.
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)