Review
Chemistry, Multidisciplinary
Xiuqiang Li, Wanrong Xie, Jia Zhu
Summary: Interfacial solar steam/vapor technology uses solar energy and water to achieve heating and cooling, which is a promising solution to environmental and energy issues. By designing and optimizing materials, structures, and devices, higher conversion and utilization efficiency can be achieved. This review summarizes recent developments and future trends in this field and discusses unsolved scientific and technical problems.
Article
Food Science & Technology
Panattida Edward, Thanakorn Wongprasert, Thasorn Bunyakanchana, Panattida Siripitakpong, Kotchakorn Supabowornsathit, Tirayut Vilaivan, Inthawoot Suppavorasatit
Summary: This study extracted protein from commercial cricket powder and found that adding ammonium sulfate changed its physicochemical and functional properties. The cricket protein isolate had a high protein content, low solubility, and showed good foaming properties and oil-holding capacity.
Article
Biochemistry & Molecular Biology
Marcel Hanke, Niklas Hansen, Ruiping Chen, Guido Grundmeier, Karim Fahmy, Adrian Keller
Summary: DNA origami technology allows for the folding of DNA strands into complex nanoscale shapes, with properties that differ significantly from genomic DNA. This study investigates the precipitation of DNA origami structures using ammonium sulfate, commonly used in protein precipitation. The results show that DNA origami nanostructures can be precipitated by centrifugation with 3 M ammonium sulfate but not double-stranded genomic DNA. The precipitated DNA origami structures can be resuspended without loss of integrity. Further, this method can be used to separate DNA origami triangles from genomic DNA fragments in a complex mixture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Anthony J. Devlin, Courtney J. Mycroft-West, Jeremy E. Turnbull, Marcelo Andrade de Lima, Marco Guerrini, Edwin A. Yates, Mark A. Skidmore
Summary: Heparin, a polydisperse and heterogeneous polysaccharide of the glycosaminoglycan (GAG) class, is widely used as a potent anticoagulant and is classified as an essential medicine by the World Health Organization. The contamination crisis in 2008 highlighted the need for rigorous monitoring and quality control of pharmaceutical heparin. Multidimensional nuclear magnetic resonance (NMR) spectroscopy techniques and multivariate analysis emerged as the gold standard, but an alternative approach utilizing attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) combined with multivariate analysis has been developed. This method enables rapid, cost-effective monitoring and analysis of pharmaceutical heparin, making it suitable for future deployment throughout the supply chain.
ACS CENTRAL SCIENCE
(2023)
Article
Medicine, Legal
Can Hu, Hongcheng Mei, Hongling Guo, Ziyang Yu, Jun Zhu
Summary: The purification of ammonium nitrate (AN) samples through recrystallization has been shown to enhance stable isotope ratios and accurately distinguish AN samples from different cities and batches.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Min Sub Sim, Connor T. Skennerton, Victoria J. Orphan
Summary: The study describes the growth kinetics and sulfur isotope effects of a methanol-oxidizing sulfate-reducing deltaproteobacterium, Desulfovibrio carbinolicus, which can utilize a series of alcohols from methanol to butanol for growth. However, specific growth and respiration rates decrease significantly when methanol is used as an electron donor. Analysis of genomes of 52 sulfate-reducing bacteria shows no clear relationship between phylogeny and methanol metabolizing capacity, with most gram-negative methanol oxidizers growing poorly.
Article
Chemistry, Physical
Xianghao Meng, Guoliang Xie, Wenli Xue, Yilei Fu, Rui Wang, Xinhua Liu
Summary: A Cu-Ni-Si alloy with excellent mechanical properties and electrical conductivity is successfully fabricated by HCCM continuous casting and two steps of aging treatment, and the effect of Cr addition is studied.
Article
Chemistry, Applied
Peineng Zhu, Weijuan Huang, Lingyun Chen
Summary: This study reported a transparent and thermo-reversible gel prepared with pea protein isolate extracted using ammonium sulfate precipitation method. The protein concentration and pH were found to be critical factors for gel formation, and within a certain range, thermo-reversible gels with good mechanical properties could be obtained. The gel's thermo-reversibility was stable upon repeated heating and cooling processes. Scanning electron microscopy showed that gels formed at lower pH had a transparent fine-stranded network structure, while increasing pH resulted in an opaque gel with particulate aggregates, while still maintaining thermo-reversibility. Hydrogen bonds were found to play a dominant role in gel network formation, while hydrophobic interactions and disulfide bonds were not significantly involved.
FOOD HYDROCOLLOIDS
(2022)
Article
Engineering, Chemical
Jaebeom Park, Wooram Lee, Jong Kwon Choe, Yongju Choi
Summary: Various methods for separating and recovering ammonia have been developed, but traditional evaporative crystallization methods have limitations. This study explores the use of solvent-driven fractional crystallization (SDFC) as an alternative technique to improve the purity and usability of recovered ammonia.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
Su In Lee, Hye Rim Kim, Jae Kwan Park, Wonzin Oh, Jeongju Kim, Chorong Kim, Junghyun Lee, Ki-Chul Kim, Byung-Chul Lee
Summary: This study experimentally investigates the precipitation kinetics of ferrous oxalate, examining the effects of the concentration of ferrous ammonium sulfate and oxalic acid as raw materials. The results demonstrate that increasing the concentration of ferrous ammonium sulfate accelerates the initial precipitation of ferrous oxalate, while the concentration of oxalic acid does not significantly affect the precipitation rate.
Article
Chemistry, Physical
Yilei Fu, Guoliang Xie, Fan Zhao, Jinfeng Wan, Xianghao Meng, Xiao Liu, Rui Wang, Xinhua Liu
Summary: A Cu-3.0 wt% Ti alloy was successfully fabricated using a novel non-vacuum short-process based on heating-cooling combined mold continuous casting method. The alloy showed comparable properties to the counterpart alloy prepared by conventional vacuum smelting. The precipitation hardening of beta'-Cu4Ti phase was found to contribute to the strengthening mechanism in this alloy.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Chemistry, Inorganic & Nuclear
Mingyang Liu, Jingbao Lian, Nianchu Wu, Xue Zhang, Jiao He
Summary: A series of Eu3+ doped pompon-like Lu2O2SO4 phosphors with diameter of about 800 nm were synthesized using lutetium oxide, nitric acid, europium nitrate, urea, ammonium sulfate, and ammonia as starting materials. The optimal molar ratio and calcination conditions were found to significantly affect the product performance. Investigation into the morphology mechanism of the pompon-like Lu2O2SO4 revealed the formation process and growth mechanisms of the unique structure.
SOLID STATE SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
S. L. Semiatin, N. C. Levkulich, R. Larsen, J. S. Tiley, K. N. Wertz, F. Zhang, T. M. Smith, R. Y. Zhang, H. B. Dong, P. Gadaud, J. Cormier
Summary: Experimental tools and fast-acting, numerical-simulation techniques were used to quantify the precipitation behavior of three nickel-base superalloys: IN-100, LSHR, and 718. The results showed semi-quantitative agreement between experimental data and simulation predictions.
METALLURGICAL AND MATERIALS TRANSACTIONS A-PHYSICAL METALLURGY AND MATERIALS SCIENCE
(2021)
Article
Geosciences, Multidisciplinary
Daniel H. James, Harold J. Bradbury, Gilad Antler, Zvi Steiner, Alec M. Hutchings, Xiaole Sun, Raoul Saar, Mervyn Greaves, Alexandra Turchyn
Summary: The pore fluid geochemistry data from the Gulf of Aqaba and the Iberian Margin show strong correlations among calcium, magnesium, strontium, and sulfate concentrations, indicating active changes in redox state and pH. Despite changes in other major ions and their isotopic composition, the calcium isotope composition of pore fluid calcium remains relatively stable in our measured profiles.
FRONTIERS IN EARTH SCIENCE
(2021)
Article
Physics, Multidisciplinary
Irfan Kaya, Rauf Nematli, Mutlu Karasoglu, Erdem Ozyurt, Emre Acar
Summary: This study investigates the effect of heating-cooling rate and cooling method after aging on the thermal properties of Ni-rich NiTiNb alloy. The results show that the transformation temperatures of the alloy vary depending on the cooling method.
EUROPEAN PHYSICAL JOURNAL PLUS
(2022)
Review
Neurosciences
Rachel E. Lackie, Andrzej Maciejewski, Valeriy G. Ostapchenko, Jose Marques-Lopes, Wing-Yiu Choy, Martin L. Duennwald, Vania F. Prado, Marco A. M. Prado
FRONTIERS IN NEUROSCIENCE
(2017)
Article
Chemistry, Physical
Mikko Karttunen, Wing-Yiu Choy, Elio A. Cino
JOURNAL OF PHYSICAL CHEMISTRY B
(2018)
Article
Biochemistry & Molecular Biology
Rachel E. Lackie, Abdul R. Razzaq, Sali M. K. Farhan, Lily R. Qiu, Gilli Moshitzky, Flavio H. Beraldo, Marilene H. Lopes, Andrzej Maciejewski, Robert Gros, Jue Fan, Wing-Yiu Choy, David S. Greenberg, Vilma R. Martins, Martin L. Duennwald, Jason P. Lerch, Hermona Soreq, Vania F. Prado, Marco A. M. Prado
JOURNAL OF NEUROCHEMISTRY
(2020)
Article
Neurosciences
Rachel E. Lackie, Jose Marques-Lopes, Valeriy G. Ostapchenko, Sarah Good, Wing-Yiu Choy, Patricija Van Oosten-Hawle, Stephen H. Pasternak, Vania F. Prado, Marco A. M. Prado
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2020)
Article
Chemistry, Physical
Megan Chang, Carter J. Wilson, Nadun Chanaka Karunatilleke, Mohamed Hesham Moselhy, Mikko Karttunen, Wing-Yiu Choy
Summary: This study elucidated the free-state structures of Nrf2's Neh4 and Neh5 domains, revealing different binding mechanisms and consistency differences between them.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2021)
Article
Biochemistry & Molecular Biology
Carter J. Wilson, Megan Chang, Mikko Karttunen, Wing-Yiu Choy
Summary: The study investigated the effects of 12 different cancer mutations on the protein KEAP1, providing structural insights into the mutants and suggesting stability as a possible target of cancer in KEAP1. The most prevalent mutation, R470C, showed no significant change in structural stability or NRF2 binding site dynamics, possibly indicating a covalent modification as its mode of action.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nadun C. Karunatilleke, Courtney S. Fast, Vy Ngo, Anne Brickenden, Martin L. Duennwald, Lars Konermann, Wing-Yiu Choy
Summary: Nrf2 is a crucial transcription regulator that plays a key role in the cellular response to oxidative stress, with implications in chemoresistance and tumorigenesis. However, the molecular details of Nrf2 and its interactions with most targets remain unknown, hindering drug design efforts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Vy Ngo, Anne Brickenden, Hansen Liu, Cynthia Yeung, Wing-Yiu Choy, Martin L. Duennwald
Summary: Nrf2 is a key transcriptional regulator in cellular responses to oxidative stress and is regulated by Keap1. It is also influenced by other protein interactions. Yeast models expressing Nrf2 and other interacting proteins are valuable tools for studying Nrf2 regulation. In this study, Nrf2 interactions, including its interaction with Hsp90, were investigated using yeast models, providing new insights into the crosstalk between antioxidant response and heat shock response.
DISEASE MODELS & MECHANISMS
(2022)
Article
Biochemistry & Molecular Biology
Vy Ngo, Nadun C. Karunatilleke, Anne Brickenden, Wing-Yiu Choy, Martin L. Duennwald
Summary: Cells respond to oxidative stress by inducing antioxidant proteins through the activation of Nrf2. Both Nrf2 and Keap1 are susceptible to protein misfolding under oxidative stress, potentially due to their intrinsic characteristics. This study provides new insights into the regulation of Nrf2 and Keap1 by oxidation-induced protein misfolding.
Review
Biochemistry & Molecular Biology
Ahmed Salem, Carter J. Wilson, Benjamin S. Rutledge, Allison Dilliott, Sali Farhan, Wing-Yiu Choy, Martin L. Duennwald
Summary: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease associated with protein misfolding and inclusion formation. Mutations in Matrin3, a nuclear DNA/RNA-binding protein, have been found to cause familial ALS. Matrin3 lacks a prion-like domain but contains long intrinsically disordered regions that may facilitate interactions with other proteins and contribute to its misfolding.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Carter J. Wilson, Wing-Yiu Choy, Mikko Karttunen
Summary: The development of AlphaFold2 has brought a paradigm shift in structural biology and has shown excellent performance in predicting disordered regions. The accuracy of the constructed disorder predictor from AlphaFold2 structures varies, with the naive assumption of assigning residues to ordered or disordered based on helices, strands, and H-bond stabilized turns leading to a significant overestimation of disorder. The predicted local distance difference test (pLDDT) provides a reliable measure of residue-wise disorder and shows an interesting relationship with secondary structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Benjamin S. Rutledge, Wing-Yiu Choy, Martin L. Duennwald
Summary: The accumulation of misfolded proteins is a characteristic feature of neurodegenerative diseases. Molecular chaperones like Hsp70 and Hsp90 play a role in defending cells against this accumulation, but the exact mechanisms are still unclear. The functions of these chaperones in protein folding and preventing protein aggregation may be important for modulating neurodegeneration.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Clinical Neurology
Rachel E. Lackie, Aline S. de Miranda, Mei Peng Lim, Vladislav Novikov, Nimrod Madrer, Nadun C. Karunatilleke, Benjamin S. Rutledge, Stephanie Tullo, Anne Brickenden, Matthew E. R. Maitland, David Greenberg, Daniel Gallino, Wen Luo, Anoosha Attaran, Irina Shlaifer, Esther Del Cid Pellitero, Caroline Schild-Poulter, Thomas M. Durcan, Edward A. Fon, Martin Duennwald, Flavio H. Beraldo, M. Mallar Chakravarty, Timothy J. Bussey, Lisa M. Saksida, Hermona Soreq, Wing-Yiu Choy, Vania F. Prado, Marco A. M. Prado
Summary: The co-chaperone STI1 interacts with alpha-synuclein and plays a role in its misfolding and aggregation. Decreased STI1 function can reduce protein inclusion formation and improve motor and cognitive deficits caused by alpha-synuclein overexpression.
ACTA NEUROPATHOLOGICA
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Vy Ngo, Nadun Karunatilleke, Zheng Song, Anne Brickenden, Milica Krstic, Wing-Yiu Choy, Martin Duennwald
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Andrzej Maciejewski, Vania F. Prado, Marco A. M. Prado, Wing-Yiu Choy
BIOCHEMICAL JOURNAL
(2017)
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)