Journal
PROTEIN ENGINEERING DESIGN & SELECTION
Volume 24, Issue 8, Pages 627-630Publisher
OXFORD UNIV PRESS
DOI: 10.1093/protein/gzr024
Keywords
biomaterials; disulfide; protein design; protein knot; topology
Funding
- National Institutes of Health [R01GM081652]
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The polypeptide backbones of a few proteins are tied in a knot. The biophysical effects and potential biological roles of knots are not well understood. Here, we test the consequences of protein knotting by taking a monomeric protein, carbonic anhydrase II, whose native structure contains a shallow knot, and polymerizing it end-to-end to form a deeply and multiply knotted polymeric filament. Thermal stability experiments show that the polymer is stabilized against loss of structure and aggregation by the presence of deep knots.
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