Journal
PROTEIN AND PEPTIDE LETTERS
Volume 16, Issue 3, Pages 320-323Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/092986609787601679
Keywords
cell proliferation; CHO-K1 cell; grass carp; growth hormone level; growth hormone receptor
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Funding
- Ministry of Science and Technology of China [2007CB707804]
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A novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR). Bioactivity assay revealed that silver carp growth hormone (scGH) bound gcGHR with dissociation constant (K-d) value of 1.96 nM and stimulated CHO-gcGHR cells proliferation with the sensitivity of 20 ng/ml. The cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein.
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