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Biochemistry & Molecular Biology
Sarah Mermet, Maxime Voisin, Joris Mordier, Tristan Dubos, Sylvie Tutois, Pierre Tuffery, Celia Baroux, Kentaro Tamura, Aline Probst, Emmanuel Vanrobays, Christophe Tatout
Summary: This study reveals the interaction mechanism between the Arabidopsis nucleoskeleton protein KAKU4 and the nuclear periphery, and identifies conserved peptide motifs that play a role in nuclear shape and gene expression. Additionally, the study finds these motifs in nuclear pore basket proteins, suggesting a physical continuum between the nuclear pore and the nucleoskeleton in plants.
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Biochemistry & Molecular Biology
Lucie Kriegshauser, Samuel Knosp, Etienne Grienenberger, Kanade Tatsumi, Desiree D. Guetle, Iben Sorensen, Laurence Herrgott, Julie Zumsteg, Jocelyn K. C. Rose, Ralf Reski, Daniele Werck-Reichhart, Hugues Renault
Summary: The HCT gene plays a critical role in the phenylpropanoid pathway in the moss species Physcomitrium patens, similar to its function in seed plants. Phylogenetic analysis suggests deep conservation of HCT function across embryophytes. Enzyme assays and metabolic profiling indicate that P. patens HCT is involved in the production of caffeate derivatives and cuticle formation in bryophytes.
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Biochemistry & Molecular Biology
Chetana Tamadaddi, Vinay Sagar, Amit K. Verma, Fathima Afsal, Chandan Sahi
Summary: Our study reveals that selective interactions between JDPs in plant mitochondria may determine their functional specificity and provide resilience to protein import processes in mitochondria.
PLANT MOLECULAR BIOLOGY
(2021)
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Multidisciplinary Sciences
Huihui Yu, Mu Li, Jaspreet Sandhu, Guangchao Sun, James C. Schnable, Harkamal Walia, Weibo Xie, Bin Yu, Jeffrey P. Mower, Chi Zhang
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NATURE COMMUNICATIONS
(2022)
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Biochemistry & Molecular Biology
Shuai Fu, Kun Wang, Tingting Ma, Yan Liang, Zhonghua Ma, Jianxiang Wu, Yi Xu, Xueping Zhou
Summary: This study identifies a microtubule-associated E3 ligase (MEL) that promotes the degradation of a serine hydroxymethyltrasferase (SHMT1), resulting in broad-spectrum resistance to pathogens. The findings suggest the potential utility of the MEL-SHMT1 module in generating broad-spectrum resistant rice to global destructive pathogens.
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Cell Biology
Yuichi Morozumi, Ai Hishinuma, Suguru Furusawa, Fajar Sofyantoro, Hisashi Tatebe, Kazuhiro Shiozaki
Summary: In this study, a TOS motif was identified in the fission yeast S6 kinase Psk1, which interacts with the RAPTOR ortholog Mip1, suggesting an evolutionarily conserved mechanism of substrate recognition by TORC1 through the interaction of TOS motifs.
JOURNAL OF CELL SCIENCE
(2021)
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Cell Biology
Sapan Borah, David J. Thaller, Zhanna Hakhverdyan, Elisa C. Rodriguez, Anthony W. Isenhour, Michael P. Rout, Megan C. King, C. Patrick Lusk
Summary: Integral membrane proteins of the Lap2-emerin-MAN1 (LEM) family are important for the inner nuclear membrane (INM). This study shows that Heh2/Man1 interacts with major components of the nuclear pore complex (NPC) in distant yeasts, with stable interactions mediated by a C-terminal winged helix domain. In addition, Heh2 acts as a sensor of NPC assembly state, crucial for NPC quality control mechanisms and segregation during cell division.
MOLECULAR BIOLOGY OF THE CELL
(2021)
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Biochemistry & Molecular Biology
Yuta Hiragori, Hiro Takahashi, Taihei Karino, Atsushi Kaido, Noriya Hayashi, Shun Sasaki, Kodai Nakao, Taichiro Motomura, Yui Yamashita, Satoshi Naito, Hitoshi Onouchi
Summary: This study identified four novel non-AUG-initiated upstream open reading frames (uORFs) with evolutionarily conserved sequences in Arabidopsis and elucidated the mechanism by which a non-AUG-initiated uORF promotes the translation of the main open reading frame (mORF). Through a combination of bioinformatics and experimental analysis, regulatory non-AUG uORFs that are physiologically important were identified and their effects on mORF expression were examined. These findings provide insights into the mechanisms that enable non-AUG uORFs to play regulatory roles despite their low translation initiation efficiencies.
PLANT MOLECULAR BIOLOGY
(2023)
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Multidisciplinary Sciences
Sophie Tissot, Lena Guimard, Jordan Meliani, Justine Boutry, Antoine M. Dujon, Jean-Pascal Capp, Jacint Tokolyi, Peter A. Biro, Christa Beckmann, Laura Fontenille, Nam Do Khoa, Rodrigo Hamede, Benjamin Roche, Beata Ujvari, Aurora M. Nedelcu, Frederic Thomas
Summary: Changes in food availability may be an important factor affecting tumor occurrence and progression. Frequent overfeeding promotes tumor emergence, while lean diets may have a protective effect.
SCIENTIFIC REPORTS
(2023)
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Biochemistry & Molecular Biology
Markus Henninger, Lorenzo Pedrotti, Markus Krischke, Jan Draken, Theresa Wildenhain, Agnes Fekete, Filip Rolland, Martin J. Mueller, Christian Froeschel, Christoph Weiste, Wolfgang Droege-Laser
Summary: This study investigates the role of the energy master regulator SnRK1 in seedling establishment in Arabidopsis thaliana, revealing its crucial function in resource mobilization and metabolic regulation. Specifically, SnRK1 promotes glucose utilization, regulates the breakdown of sucrose, triacylglycerols, and amino acids, and activates gene expression through phosphorylation of transcription factors.
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Biology
Elin Karlsson, Frieda A. Sorgenfrei, Eva Andersson, Jakob Dogan, Per Jemth, Celestine N. Chi
Summary: The evolution of proteins is constrained by their structure and function. A recent study on the Nuclear Coactivator Binding Domain (NCBD) from CREB-binding protein reveals that although it has increased thermodynamic stability during evolution, its dynamic biophysical properties have been maintained. These findings suggest that the dynamic properties of NCBD are important for its function.
COMMUNICATIONS BIOLOGY
(2022)
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Cell Biology
Aseem Shrivastava, Carl Alexander Sandhof, Kevin Reinle, Areeb Jawed, Carmen Ruger-Herreros, Dominic Schwarz, Declan Creamer, Carmen Nussbaum-Krammer, Axel Mogk, Bernd Bukau
Summary: Sequestration of misfolded proteins is an evolutionarily conserved activity of the sHsp family and is executed by specific family members. Certain sHsps in Caenorhabditis elegans have specific sequence features and can buffer limitations in sequestrase capacity, contributing to lifespan extension.
JOURNAL OF CELL BIOLOGY
(2022)
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Biotechnology & Applied Microbiology
Shrey Gandhi, Anika Witten, Federica De Majo, Martijn Gilbers, Jos Maessen, Ulrich Schotten, Leon J. de Windt, Monika Stoll
Summary: The study revealed evolutionarily conserved transcription patterns within different heart chambers, with most lncRNAs conserved by synteny. Regional co-expression patterns of transcripts are modulated by factors such as genomic overlap, strandedness, and transcript biotype. The research provides a community resource called EvoACTG, informing researchers about the conserved yet intertwined nature of the coding and non-coding cardiac transcriptome across popular model organisms in CVD research.
Article
Multidisciplinary Sciences
Susanne Bornelov, Benjamin Czech, Gregory J. Hannon
Summary: Piwi-interacting RNAs (piRNAs) are small RNAs produced by processing of long precursor transcripts. The authors discovered that piRNA precursor cleavage occurs at stop codon positions and this pattern is conserved across mammals.
NATURE COMMUNICATIONS
(2022)
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Biochemistry & Molecular Biology
Paulina Stachula, Katarzyna Kapela, Ewelina Malecka, Kamila Jaronczyk, Jacek Patryn, Nina Siwirykow, Maria Bucholc, Malgorzata Marczak, Maciej Kotlinski, Rafal Archacki
Summary: In this study, the organization of Arabidopsis SWI/SNF complexes formed around a BRM catalytic subunit was elucidated, and the requirement of bromodomain-containing proteins BRD1/2/13 for the formation and stability of the entire complex was defined. The BRM complexes strongly resemble mammalian non-canonical BAF complexes, and the BDH1/2 proteins were identified as components of the BRM complex important for plant development and hormone responses. The unique subunits BRD1/2/13 were found to be essential for the integrity of the complex, and the existence of a module consisting of the ATPase, ARP, and BDH proteins in a BRD-dependent manner was revealed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
