Article
Biochemistry & Molecular Biology
Jong-Kwang Kim, Jae-Hun Jung, Dong-Hoon Shin, Hye-Jin You, Seho Cha, Bo-Seul Song, Jae-Young Joung, Weon-Seo Park, Kwang-Pyo Kim, Jae-Kyung Myung
Summary: This study characterized the rapid proteomic changes in VCaP cells after androgen treatment, identifying five protein clusters involved in different stages of androgen-initiated signal transmission and processing. The study revealed significant regulation of AR-regulated proteins in CRPC patients and provided a comprehensive perspective on the molecular mechanisms underlying CRPC progression.
Article
Oncology
Yanhong Liu, Lan Gao, Yanru Fan, Rufei Ma, Yunxia An, Guanghui Chen, Yan Xie
Summary: Potential protein biomarkers and mechanism of venous thromboembolism (VTE) in non-small cell lung cancer (NSCLC) patients were identified through proteomics research. A total of 280 differentially expressed proteins were found, and 5 proteins (SAA1, S100A8, LBP, HP and LDHB) showed significant changes between VTE and non-VTE patients. These results suggest that these proteins may be used for diagnosing VTE in NSCLC patients.
FRONTIERS IN ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
John P. McGee, Pei Su, Kenneth R. Durbin, Michael A. R. Hollas, Nicholas W. Bateman, G. Larry Maxwell, Thomas P. Conrads, Ryan T. Fellers, Rafael D. Melani, Jeannie M. Camarillo, Jared O. Kafader, Neil L. Kelleher
Summary: This study introduces a semi-automated single-ion mass spectrometry workflow for top-down tandem mass spectrometry analysis directly from tissue microenvironments. The results demonstrate that this method can identify multiple proteoforms in tumor samples within a short period of time, and effectively identify proteoform signatures in tumor and stromal regions.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Tiziano Marzo, Giarita Ferraro, Lorena Maria Cucci, Alessandro Pratesi, Orjan Hansson, Cristina Satriano, Antonello Merlino, Diego La Mendola
Summary: The study shows that the anti-cancer drug oxaliplatin efficiently binds angiogenin, inhibiting its promoting effects on cell proliferation and migration. This suggests angiogenin as a potential target of oxaliplatin, indicating a new mechanism for the anti-neoplastic activity of this platinum drug and opening avenues for novel approaches in combined anti-cancer anti-angiogenic therapy.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Urology & Nephrology
Melanie Rouleau, Bertrand Neveu, Patrick Caron, Fannie Morin, Paul Toren, Louis Lacombe, Veronique Turcotte, Eric Levesque, Chantal Guillemette, Frederic Pouliot
Summary: Testosterone and dihydrotestosterone play a significant role in prostate cancer progression and resistance. Castration reduces the levels of 15 circulating steroids, and their association with testosterone and dihydrotestosterone varies under different treatment conditions.
JOURNAL OF UROLOGY
(2022)
Article
Oncology
Ammara Muazzam, Davide Chiasserini, Janet Kelsall, Nophar Geifman, Anthony D. Whetton, Paul A. Townsend
Summary: Prostate cancer is the third most common cancer in men globally. The PSA is the main protein biomarker used for diagnosis, staging, and monitoring, but it can lead to inaccurate diagnosis and overtreatment. Therefore, identifying novel biomarkers for prostate cancer can help improve the understanding of diagnosis and risk assessment.
Article
Biochemical Research Methods
Sunghyun Huh, Chaewon Kang, Ji Eun Park, Dowoon Nam, Se Ik Kim, Aeran Seol, Kyerim Choi, Daehee Hwang, Myeong-Hee Yu, Hyun Hoon Chung, Sang-Won Lee, Un-Beom Kang
Summary: This study identified potential diagnostic biomarkers for HGSOC using liquid chromatography data-independent acquisition tandem mass spectrometry. The findings revealed activations and suppressions of specific signaling pathways in HGSOC, and validation in an independent cohort showed promising sensitivity and specificity. Functional assays on selected biomarkers suggested their roles in cancer cell proliferation and migration in HGSOC.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Multidisciplinary Sciences
Lin Lei, Lijuan Yu, Weixiao Fan, Xiaoke Hao
Summary: This study reveals the significant role of small extracellular vesicles (sEVs) in the androgen-independent transformation of prostate cancer. By transferring the key sEVs molecule Let-7a-5p and activating the androgen receptor (AR) and PI3K/Akt signaling pathways, sEVs regulate the process. This finding provides new perspectives for targeted treatment approaches for AIPC patients.
Review
Spectroscopy
Reta Birhanu Kitata, Jhih-Ci Yang, Yu-Ju Chen
Summary: Data-independent acquisition mass spectrometry (DIA-MS) is a highly reproducible proteome profiling method that generates permanent digital maps for retrospective analysis. Recent advancements have improved the sensitivity and coverage of DIA-MS. This review discusses the evolution of DIA-MS techniques, recent applications, and challenges.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Demitria M. Vasilatis, Christopher A. Lucchesi, Paramita M. Ghosh
Summary: Dogs naturally develop prostate cancer similar to aggressive forms found in humans. Prostate cancer samples in dogs often lack androgen receptor (AR), which can enhance our understanding of AR-indifferent prostate cancer in humans. This review highlights the molecular similarities between dog and human prostate cancer variants, suggesting the potential use of dogs as pre-clinical animal models for developing new therapies and diagnostics that can benefit both species.
Review
Cell Biology
Ujjwal R. Dahiya, Hannelore Heemers
Summary: The androgen receptor (AR) plays a crucial role in male phenotype development and diseases, including prostate cancer. Therapeutic approaches targeting the interaction between AR and its ligands show promise, but face challenges. Understanding the 3D structure of AR complexes and protein interaction sites is essential for developing effective interventions.
Article
Oncology
Tania Lima, Rui Henrique, Rui Vitorino, Margarida Fardilha
Summary: Prostate cancer (PCa) is a common cancer among men, but detection through prostate-specific antigen has limitations. This study aimed to identify consistent molecular features and potential urinary biomarkers for PCa by comparing urinary and tissue proteomes to exclude interference from kidney and bladder cancer. The analysis revealed dysregulated molecular processes in urine from PCa patients, leading to the identification of candidate urinary biomarkers such as MSMB, KLK3, ITIH4, ITIH2, HPX, GP2, APOA2, and AZU1 proteins.
Article
Biochemical Research Methods
Patricia Skowronek, Florian Krohs, Markus Lubeck, Georg Wallmann, Ericka C. M. Itang, Polina Koval, Maria Wahle, Marvin Thielert, Florian Meier, Sander Willems, Oliver Raether, Matthias Mann
Summary: Data-independent acquisition (DIA) methods have benefits of continuous acquisition of fragment spectra for all precursors simultaneously, but reconstructing the precursor-fragment relationships in convoluted spectra poses challenges. In this study, synchro-PASEF scan mode is introduced, combining trapped ion mobility spectrometry and parallel accumulation-serial fragmentation (PASEF) to accurately define precursor-fragment relationships and deconvolute the DIA fragment space. The synchro-PASEF method increases fragment ion current and provides a lock-and-key mechanism for specificity in quantification. Overall, this research establishes the defining features of synchro-PASEF and explores its potential for proteomic analyses.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Hyunho Han, Cheol Keun Park, Young-Deuk Choi, Nam Hoon Cho, Jongsoo Lee, Kang Su Cho
Summary: Prostate cancer is a common cancer in men, and some patients develop resistance to androgen-deprivation therapy (ADT), resulting in castration-resistant prostate cancer (CRPC). This study found that statins can selectively inhibit the growth of CRPC tumors that have lost their androgen receptor (AR) and overexpressed the RNA-binding protein QKI. The research also revealed a potential mechanism behind the efficacy of statins and suggested a biology-driven drug-repurposing clinical trial to improve treatment options for this aggressive form of prostate cancer.
Review
Biochemistry & Molecular Biology
Joohyun Ryu, Stefani N. Thomas
Summary: Ovarian cancer is a deadly gynecologic malignancy with the need for new and more effective treatment strategies, while early detection and protein biomarkers play crucial roles. Targeted proteomics methods, including mass spectrometry, offer robust solutions for the development of ovarian cancer biomarkers.
Editorial Material
Medical Laboratory Technology
Eleftherios P. Diamandis, Karl J. Lackner, Mario Plebani
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Editorial Material
Medicine, General & Internal
Panos Macheras, Caitlin Di Paolo, Yanna Pusica, Eleftherios P. Diamandis
Letter
Medical Laboratory Technology
Oscar D. Pons-Belda, Amaia Fernandez-Uriarte, Annie Ren, Eleftherios P. Diamandis
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Biochemical Research Methods
Stella K. Vasiliou, Panagiota S. Filippou, Sergi Clotet-Freixas, Antoninus Soosaipillai, Ihor Batruch, Foivos Viktor Tsianos, Ana Konvalinka, Eleftherios P. Diamandis
Summary: This study found that AR regulates the metabolism of BT-474 cells by affecting the expression of a large number of genes and proteins. Further pathway analysis suggests that the androgen receptor acts as a tumor suppressor in BT-474 cells.
CLINICAL PROTEOMICS
(2022)
Letter
Medicine, General & Internal
Eleftherios P. Diamandis, Sok-Ja Janket, Harry A. Conte
Article
Biophysics
I. Novodchuk, M. Kayaharman, I. Prassas, A. Soosaipillai, R. Karimi, I. A. Goldthorpe, E. Abdel-Rahman, J. Sanderson, E. P. Diamandis, M. Bajcsy, M. Yavuz
Summary: Researchers have developed a graphene-based FET biosensor with high sensitivity and fast detection capabilities for detecting SARS-CoV-2 nucleocapsid protein. The biosensor can detect the viral protein within four minutes with a wide detection range.
BIOSENSORS & BIOELECTRONICS
(2022)
Letter
Medical Laboratory Technology
Eleftherios. P. P. Diamandis
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Ivan Pasic, Annie H. Ren, Ram Vasudevan Nampoothiri, Ioannis Prassas, Jeffrey H. Lipton, Jonas Mattsson, Eleftherios P. Diamandis, Fotios V. Michelis
Summary: Using a novel multiplex antibody-based proximity extension assay, researchers identified serum proteins, including SLAMF7, IL-1ra, BTN3A2, and DAB2, that may potentially serve as biomarkers for acute graft-vs.-host disease.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Annie H. Ren, Panagiota S. Filippou, Antoninus Soosaipillai, Lampros Dimitrakopoulos, Dimitrios Korbakis, Felix Leung, Vathany Kulasingam, Marcus Q. Bernardini, Eleftherios P. Diamandis
Summary: This study explored the potential of using MUC13 as an ovarian cancer biomarker to complement CA125. The results showed that MUC13 could be used to detect non-serous ovarian carcinoma and early-stage disease, in addition to CA125.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Hannu Koistinen, Ruusu-Maaria Kovanen, Morley D. Hollenberg, Antoine Dufour, Evette S. Radisky, Ulf-Hakan Stenman, Jyotsna Batra, Judith Clements, John D. Hooper, Eleftherios Diamandis, Oliver Schilling, Antti Rannikko, Tuomas Mirtti
Summary: Since the proposition of the pro-invasive activity of proteolytic enzymes over 70 years ago, several roles for proteases in cancer progression have been established. About half of the 473 active human proteases are expressed in the prostate and many of the most well-characterized members of this enzyme family are regulated by androgens, hormones essential for development of prostate cancer. Most notably, several kallikrein-related peptidases, including KLK3 (prostate-specific antigen, PSA), the most well-known prostate cancer marker, and type II transmembrane serine proteases, such as TMPRSS2 and matriptase, have been extensively studied and found to promote prostate cancer progression. Recent findings also suggest a critical role for proteases in the development of advanced and aggressive castration-resistant prostate cancer (CRPC). Perhaps the most intriguing evidence for this role comes from studies showing that the protease-activated transmembrane proteins, Notch and CDCP1, are associated with the development of CRPC.
Letter
Medical Laboratory Technology
Eleftherios P. Diamandis
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Oncology
Timo-Pekka K. Lehto, Ruusu-Maaria Kovanen, Susanna Lintula, Adrian Malen, Carolin Sturenberg, Andrew Erickson, Olli-Pekka Pulkka, Ulf-Hakan Stenman, Eleftherios P. Diamandis, Antti Rannikko, Tuomas Mirtti, Hannu Koistinen
Summary: This study aimed to investigate the mRNA levels and prognostic impact of all 15 human kallikrein-related peptidases (KLKs) and their targets, proteinase-activated receptors (PARs), in surgically treated prostate cancer (PCa). The results showed that the expression of KLK2, KLK3, KLK4, and KLK15 was closely associated with tumor aggressiveness and prognosis, suggesting their potential as prognostic biomarkers for PCa.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Letter
Medical Laboratory Technology
Eleftherios P. Diamandis
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Letter
Medicine, General & Internal
Sok-Ja Janket, Jukka H. Meurman, Eleftherios P. Diamandis
Review
Medicine, General & Internal
Arsani Yousef, Lucianna Ghobrial, Eleftherios P. Diamandis
Summary: Tumors vary in location, tissue type, and histological subtype. This review highlights current classification schemes for tumor heterogeneity and discusses its clinical significance. New molecular techniques offer affordable assessment of tumor heterogeneity, potentially revolutionizing cancer diagnosis and treatment. Standardized and reproducible assessment methods are needed to fully realize the benefits of tumor heterogeneity.