Article
Biochemistry & Molecular Biology
Adam Jugl, Miloslav Pekar
Summary: The interactions between hyaluronan and cecropin B were investigated using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. The study found that the molecular weight of hyaluronan played a significant role in the interactions, particularly the transition between different conformations. In water, interactions were saturated at a molar charge ratio of 1.5, possibly due to steric hinderance or functional group interactions. However, in PBS, no interactions were observed, regardless of the molecular weight of hyaluronan, indicating that the interactions were suppressed at higher ionic strength.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Pharmacology & Pharmacy
Iago Dillion Lima Cavalcanti, Francisco Humberto Xavier Junior, Nereide Stela Santos Magalhaes, Mariane Cajuba de Britto Lira Nogueira
Summary: Isothermal titration calorimetry (ITC) is a technique for evaluating the thermodynamic profiles of connection between two molecules, allowing the experimental design of nanoparticles systems with drugs and/or biological molecules. In the field of pharmaceutical nanotechnology, ITC has been increasingly used to understand the interaction mechanisms in the formation of nanoparticles and the behavior of nanocarriers in in vivo studies. This technique provides quick and easy results to optimize the formulation process of nano-systems.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
Falin He, Shanshan Chu, Ning Sun, Xiangxiang Li, Mingyang Jing, Jingqiang Wan, Wansong Zong, Jingchun Tang, Rutao Liu
Summary: Acrylamide interacts with lysozyme under physiological conditions, affecting the structure and function of lysozyme by unfolding polypeptide chains and decreasing alpha-helical content. The interaction is primarily driven by acrylamide's interactions with lysozyme's tryptophan and tyrosine residues, and directly affects the enzyme's active center and fluorescence sensitization phenomenon.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Munira Khatun, Gopal Chandra Jana, Sk Nayim, Asima Dhal, Anirudha Patra, Maidul Hossain
Summary: This report discusses the structural and thermodynamic characteristics of the interaction between four 9-O-substituted berberines and CT-DNA. The analogues, except BRDR2, showed partly intercalative binding to DNA. Hydrophobic interaction plays a crucial role in this process. The complete multispectroscopic and thermodynamic binding studies are useful for drug design and development.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Shams T. A. Islam, Jie Zhang, Fabio Tonin, Renske Hinderks, Yanthi N. Deurloo, Vlada B. Urlacher, Peter-Leon Hagedoorn
Summary: Lignin valorization through laccase catalysis can release aromatic products, but different enzymes exhibit significant variations in conversion efficiency and rate. Trametes versicolor laccase follows Michaelis-Menten kinetics in converting Kraft lignin, with specific thermodynamic and kinetic parameters, making it a promising tool for guiding enzymatic lignin valorization strategies.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Chemistry, Physical
Pablo F. Garrido, Margarida Bastos, Adrian Velazquez-Campoy, Alfredo Amigo, Philippe Dumas, Angel Pineiro
Summary: The injection of air into an isothermal titration calorimeter's sample cell allows for accurate determination of the surface tension of the target liquid, despite the periodic contribution from bubbles. The new algorithm successfully identifies and analyzes these contributions, improving the analysis of calorimetric signals.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Tzu-Yi Tai, Fanrui Sha, Xiaoliang Wang, Xingjie Wang, Kaikai Ma, Kent O. Kirlikovali, Shengyi Su, Timur Islamoglu, Satoshi Kato, Omar K. Farha
Summary: This study presents the first systematic investigation on the thermodynamics of protein immobilization in metal-organic frameworks (MOFs). It demonstrates that MOFs provide a hydrophobic stabilizing microenvironment for proteins, leading to an entropically driven encapsulation process. Among a series of Zr-MOFs, pyrene-based NU-1000 exhibits the most favorable encapsulation process for insulin.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Pharmacology & Pharmacy
Miao Zhang, Ning Zhang, Xinluan Lu, Wenjin Li, Ruiyong Wang, Junbiao Chang
Summary: Chlorogenic acid, as a polyphenolic compound, has antioxidant, anti-inflammatory, antiviral, anti-obesity, and other effects. This study analyzed the interaction between chlorogenic acid and four proteins (human serum albumin, pepsin, trypsin, fat mass and obesity-associated protein) and found that chlorogenic acid had a binding affinity for three proteins (human serum albumin, trypsin, and pepsin), but not for fat mass and obesity-associated protein. Hydrophobic interaction and hydrogen bonding were observed in the binding process.
PHARMACOLOGICAL REPORTS
(2022)
Article
Thermodynamics
Ziga Medos, Isidora Cobanov, Marija Bester-Rogac, Bojan Sarac
Summary: This research paper examines the issues associated with power compensation isothermal titration calorimetry, highlighting the possibility of obtaining different enthalpy values with different instruments and setting parameters. It emphasizes that modern calorimeters' dynamic power compensation mode may lead to overcompensation of heat effects, resulting in experimental results heavily reliant on chosen parameters. To avoid these problems, the paper suggests checking raw signals and conducting experiments with low or disabled power compensation when observing switching effects.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)
Article
Food Science & Technology
Siduo Zhou, Ling Meng, Yanfei Lin, Xueqian Dong, Mingsheng Dong
Summary: This study investigates the noncovalent interaction mechanisms between soybean 7S globulin and three polyphenols and finds that different compounds have different effects on the protein. Various techniques, such as thermodynamic experiments and fluorescence experiments, provide strong evidence for explaining these interactions.
Article
Chemistry, Physical
Zhihan Cui, Falin He, Xiangxiang Li, Mingyang Jing, Chengqian Huo, Wansong Zong, Rutao Liu
Summary: This study investigated the interaction mechanism between triclosan and lysozyme using various biophysical methods. The results revealed that the binding of triclosan and lysozyme enhanced fluorescence, disrupted the secondary structure of lysozyme, and induced a small amount of α-helix structure. The binding also affected the activity of lysozyme. These findings are important for understanding the toxicity of triclosan and its effects on organisms.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Multidisciplinary
Fanrui Sha, Tzu-Yi Tai, Madeleine A. Gaidimas, Florencia A. Son, Omar K. Farha
Summary: Isothermal titration calorimetry (ITC) is a technique for measuring the thermodynamic parameters of binding events, which has applications not only in biological macromolecules but also in synthetic systems like metal-organic frameworks (MOFs). Utilizing ITC in MOFs can provide insights into small molecule adsorption mechanisms and the formation of MOF-based composite materials through non-covalent interactions, with specific experimental considerations discussed. Furthermore, underexplored research directions related to MOFs that could benefit from ITC are highlighted.
Article
Polymer Science
Jacek J. Walkowiak, Rohit Nikam, Matthias Ballauff
Summary: The effective charge of highly charged polyelectrolytes is significantly lowered by a condensation of counterions. This effect is more pronounced for divalent ions. Our study on dendritic polyglycerol sulfate (dPGS) demonstrates that the interactions between dPGS and divalent ions (Mg2+ and Ca2+) show no ion specificity but clear competition between monovalent and divalent ions. These findings support the latest theoretical studies and indicate the sequential replacement of monovalent ions with divalent ions.
Article
Chemistry, Applied
Wei Liao, Emilie Dumas, Abdelhamid Elaissari, Adem Gharsallaoui
Summary: This study investigated the formation mechanism of multilayer emulsions in oil-in-water systems by using isothermal titration calorimetry (ITC) and dynamic light scattering (DLS) techniques. The results showed that ITC could provide more accurate information about the polymer concentration effect. The titration experiment confirmed that low methoxyl pectin (LMP) mainly bound sodium caseinate (CAS)-coated droplets through electrostatic forces, and stable multilayer emulsions were obtained by titrating CAS-stabilized droplets into LMP.
FOOD HYDROCOLLOIDS
(2023)
Article
Pharmacology & Pharmacy
Mohd Adnan, Debarati Dasgupta, Saleha Anwar, Anas Shamsi, Arif Jamal Siddiqui, Mejdi Snoussi, Fevzi Bardakci, Mitesh Patel, Md Imtaiyaz Hassan
Summary: This study showed that galantamine, an acetylcholinesterase inhibitor, binds to MARK4 with strong affinity and significantly inhibits its kinase activity. These results suggest that galantamine could be a potential inhibitor of MARK4 and a promising therapeutic target for AD.
FRONTIERS IN PHARMACOLOGY
(2023)
