Article
Chemistry, Multidisciplinary
Qin Guo, Xuwen Li, Wenxi Zhou, Yongchao Chu, Qinjun Chen, Yiwen Zhang, Chao Li, Hongyi Chen, Peixin Liu, Zhenhao Zhao, Yu Wang, Zheng Zhou, Yifan Luo, Chufeng Li, Haoyu You, Haolin Song, Boyu Su, Tongyu Zhang, Tao Sun, Chen Jiang
Summary: A pH-sensitive drug delivery system was designed to target different cells in TME successively, showing great potential in triple-negative breast cancer model.
Article
Astronomy & Astrophysics
K. Liu, J. Antoniadis, C. G. Bassa, S. Chen, I Cognard, M. Gaikwad, H. Hu, J. Jang, G. H. Janssen, R. Karuppusamy, M. Kramer, K. J. Lee, R. A. Main, G. Mall, J. W. McKee, M. B. Mickaliger, D. Perrodin, S. A. Sanidas, B. W. Stappers, L. Wang, W. W. Zhu, M. Burgay, R. Concu, A. Corongiu, A. Melis, M. Pilia, A. Possenti
Summary: This study reports the detection of quasi-periodic micro-structure in three millisecond pulsars using high time resolution data. The occurrence rate of micro-structure is consistent among pulses with different peak flux densities, and it often exhibits high linear polarization. The results provide valuable insights into the dependency of micro-structure properties and its potential link to fast radio bursts.
MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY
(2022)
Article
Engineering, Environmental
Chunyu Huang, Tianfu Zhang, Yang Li, Meng Lyu, Meng Suo, Ligang Xia, Lingrong Liu, Benzhong Tang, Qiqing Zhang
Summary: In this study, a tumor exosome bionic cascade catalysis system was constructed to enhance the production of active oxygen species and effectively suppress tumor growth and induce tumor immunogenic cell death. The system shows great potential for clinical applications.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Mohamad Farihan Afnan Mohd Rozi, Raja Noor Zaliha Raja Abd Rahman, Adam Thean Chor Leow, Mohd Shukuri Mohamad Ali
Summary: This study investigates the evolutionary process of bacterial lipases from family I.3 and reconstructs the last universal common ancestor (LUCA) sequence through phylogenetic tree inference and ancestral sequence reconstruction (ASR) technique. The purified LUCA exhibits high temperature and pH adaptability, as well as tolerance towards metal ions and organic solvents. The findings contribute to the understanding of temperature and environment during ancient times, and the reconstructed ancestral enzymes have improved properties for industrial applications using ASR technique as a general enzyme engineering technique.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2022)
Article
Chemistry, Applied
Mengyao Zheng, Hongmei Liu, Jin Ye, Baoxia Ni, Yanli Xie, Songxue Wang
Summary: In this study, a DNA hydrogel was used as a biosensor substrate, combined with an AFB1 aptamer and an enzyme-linked signal amplification strategy, to construct a responsive sensor for on-site detection of AFB1. The sensor exhibited outstanding sensitivity and selectivity, and the feasibility and reliability were verified in real samples.
Article
Nanoscience & Nanotechnology
Uzodinma Okoroanyanwu, Ayush Bhardwaj, James J. Watkins
Summary: We developed a method for preparing high-quality, large area, few-layer graphene films on any substrate using a xenon flash lamp. This method allows for fast and efficient synthesis of graphene without the need for catalytic substrates or conductive electrodes. The precursor films used in this method have strong absorption within the emission spectrum of the xenon flash lamp.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Jeffrey A. Purslow, Jolene N. Thimmesch, Valeria Sivo, Trang T. Nguyen, Balabhadra Khatiwada, Rochelle R. Dotas, Vincenzo Venditti
Summary: Enzyme I (EI) of the bacterial phosphotransferase system (PTS) is a master regulator of bacterial metabolism and a potential target for broad-spectrum antibiotics. Research shows that the activity of EI is influenced by the conformational dynamics within the N-terminal domain (EIN), and engineering hybrid mesophilic/thermophilic chimeras can modulate these dynamics to affect enzyme activity. This study provides insights into the dynamics/function relationship in proteins and opens new possibilities in biophysics research.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Analytical
Yang Hai-Zhu, Chu Ya-Ru, Wang Qing-Xiang, Ding Cai-Feng, Gao Feng
Summary: A novel electrochemical sensor based on MIL-101(Fe) /NH2-GR was successfully constructed for label-free detection of cTnI with a good linear range and low detection limit. The sensor showed good selectivity and reproducibility, and was applicable for detection of cTnI in human serum samples with high recoveries.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Bing Li, Alexandre Adam, Miryam Criado-Gonzalez, Loic Jierry, Joelle Bizeau, Alain Chaumont, Sebastien Harlepp, Christophe Melart, Sylvie Begin-Colin, Dominique Begin, Damien Mertz
Summary: This paper presents an original approach to trigger the self-assembly of peptide hydrogel for drug release using enzyme-coated carbon-based nanocomposites. The study shows that drug release can be effectively triggered by NIR light-induced hyperthermia temperature.
Article
Biochemistry & Molecular Biology
Mengli Cai, Ying Huang, John Lloyd, Robert Craigie, G. Marius Clore
Summary: The presented protocol allows for the expression of perdeuterated, Ile/Leu/Val H-1/C-13 methyl protonated proteins from 100 ml cultures in a cost-effective manner with comparable protein yields to conventional 1 L cultures, while using three-fold less precursors.
JOURNAL OF BIOMOLECULAR NMR
(2021)
Article
Chemistry, Physical
Vitali Tugarinov, Alberto Ceccon, G. Marius Clore
Summary: The dynamics of methyl-bearing side chains in proteins were investigated through C-13 relaxation measurements, revealing correlations and anticorrelations between parameters, which can be partially decorrelated through the analysis of different C-13 magnetization modes.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Chemistry, Multidisciplinary
Alberto Ceccon, Vitali Tugarinov, G. Marius Clore
Summary: Huntingtin polypeptides (htt(ex1)), containing an expanded polyglutamine tract encoded by exon 1 of the htt gene, form fibrils that accumulate in neuronal inclusion bodies, leading to the fatal neurodegenerative condition Huntington's disease. The binding of Fyn SH3 to the first polyproline tract of htt(ex1)Q(35) inhibits fibril formation, showcasing potential therapeutic implications in Huntington's disease.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
Thomas Schmidt, Jaekyun Jeon, Wai-Ming Yau, Charles D. Schwieters, Robert Tycko, G. Marius Clore
Summary: Recent advances in rapid mixing and freeze quenching have allowed for the study of protein-substrate interactions using time-resolved electron paramagnetic resonance (EPR)-based double electron-electron resonance (DEER) and solid-state NMR. These methods provide valuable information on the kinetic and structural pathways of protein-substrate binding.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Emma E. Cawood, G. Marius Clore, Theodoros K. Karamanos
Summary: The T193A mutation in DNAJB6 reduces self-oligomerization and anti-aggregation activity while increasing the rate of formation of a partially folded state. This highlights the importance of chaperone dynamics in regulating protein aggregation and suggests potential therapeutic avenues targeting specific substrates.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Christina Bergonzo, Alexander Grishaev, Sandro Bottaro
Summary: We describe the conformational ensemble of a single-stranded oligonucleotide using MD simulations and the FARFAR2 algorithm. The presence of A-form-like structures and other extended conformations is necessary to explain experimental data. However, simulations result in more compact structures than observed in experiments. Integration of simulations with NMR data improves the description of the conformational ensemble, but discrepancies with experimental SAXS data persist.
Review
Cell Biology
G. Marius Clore
Summary: Solution nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for analyzing three-dimensional structure and dynamics of macromolecules at atomic resolution. Recent advances in NMR have allowed the detection and characterization of transient states of biological macromolecules, which play key roles in various biological processes. This study demonstrates the application of NMR techniques in unraveling the complex mechanism of sub-millisecond oligomerization and the inhibition of fibril formation in huntingtin.
JOURNAL OF CELL SCIENCE
(2022)
Article
Chemistry, Physical
Alberto Ceccon, Nina Kubatova, John M. Louis, G. Marius Clore, Vitali Tugarinov
Summary: The global motions of ubiquitin on the surface of bicelles were studied. Ubiquitin undergoes internal rotation on the bicelle surface, while the rotation axis wobbles in a cone motion.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Multidisciplinary
Thomas Schmidt, David Wang, Jaekyun Jeon, Charles D. Schwieters, G. Marius Clore
Summary: The study demonstrates the existence of substates of calcium-loaded calmodulin that can be trapped and analyzed experimentally, revealing a complex energy landscape with varying proportions and characteristics of different structural states.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Physical
Vitali Tugarinov, Yusuke Okuno, Francesco Torricella, Theodoros K. Karamanos, G. Marius Clore
Summary: Degenerate spin systems are challenging for CPMG relaxation dispersion NMR experiments. A simple alternative method called the steady-state CPMG scheme is proposed, which utilizes acute-angle 1H radiofrequency pulses to establish a steady-state for effective rates of magnetization decay. The method is applied to two protein systems with different exchange regimes.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Yusuke Okuno, Charles D. Schwieters, Zhilin Yang, G. Marius Clore
Summary: This study explores distinct properties of solvent paramagnetic relaxation enhancement (sPRE) arising from nitroxide-based cosolutes and provides new insights into the interpretation of sPRE and sPRE-derived OENS. Four different approaches for evaluating OENS are discussed, which enable the investigation of both long-range and short-range electrostatic interactions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Mengli Cai, Vitali Tugarinov, Sai Chaitanya Chiliveri, Ying Huang, Charles D. Schwieters, Kyoshi Mizuuchi, G. Marius Clore
Summary: The bacterial MinE and MinD division regulatory proteins form a standing wave enabling MinC, which binds MinD, to inhibit FtsZ polymerization except at the midcell. The structure and dynamics of MinE bound to disc-shaped lipid bicelles are probed using NMR. In the bicelle-bound state, helix alpha 1 interacts with the bicelle surface, while the protein core remains unperturbed and also interacts with the bicelle surface via helix alpha 2. The results provide insights into MinE structural rearrangements during bacterial cell division.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Shreya Ghosh, Vitali Tugarinov, G. Marius Clore
Summary: The chaperone Hsp104 prevents fibril formation of amyloidogenic peptides in a substochiometric manner. By studying its interaction with the A(342) peptide, it was found that Hsp104 binds reversibly to sparsely populated nuclei of A(342), completely inhibiting fibril formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Physical
Yusuke Okuno, G. Marius Clore
Summary: This paper shows that it is possible to extend the frequency range of solvent paramagnetic relaxation enhancement (sPRE) experiments by acquiring data at different temperatures. By using the scaling property of spectral densities, the authors were able to accurately calculate the energy and timescale of cosolute-protein interactions. The effective near-surface potentials obtained from this method provide reliable measures of intermolecular interactions.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Multidisciplinary Sciences
Alberto Ceccon, Vitali Tugarinov, Francesco Torricella, G. Marius Clore
Summary: The tetramerization of the N-terminal region of the huntingtin protein is crucial for the development of Huntington's disease, and fibril formation occurs through nucleation and elongation processes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)