Journal
PROCESS BIOCHEMISTRY
Volume 48, Issue 4, Pages 614-619Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.03.007
Keywords
Polyamidoamine dendrimer; Microwave-assisted; Immobilized cellulase; Chitosan; Chitooligosaccharide
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Funding
- National Natural Science Foundation of China [90713013, 21075008]
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Polyamidoamine dendrimer (PAMAM) is one of a number of dendritic polymers with a precise molecular structure, high geometric symmetry, and a large number of terminal groups. In this study, PAMAM was grafted onto the surface of silica by microwave irradiation and characterized by Fourier transform infrared spectroscopy and elemental analysis. A novel immobilized cellulase was developed based on enzyme immobilization onto the prepared PAMAM-grafted silica and applied in microwave-assisted chitosan enzymolysis. The results show that the efficiency of cellulase immobilization increased with increasing generations of PAMAM. A high enzymatic hydrolysis efficiency was obtained for a 7 mg ml(-1) chitosan solution at pH 6.2 and 50 C with 40 W microwave-assisted enzymolysis (20 min) compared with a conventional enzymolysis protocol (3 h). The experimental results indicate that this rapid and efficient enzymolysis method combines the advantages of both PAMAM and microwave-assisted technology, which can be adapted to high-throughput enzyme assay in biochemical and clinical research. (C) 2013 Elsevier Ltd. All rights reserved.
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