Review
Energy & Fuels
Mohsin Raza, Labeeb Ali, Abrar Inayat, Lisandra Rocha-Meneses, Shams Forruque Ahmed, Md Mofijur, Farrukh Jamil, Chukwuma Leonard Azimoh
Summary: Biodiesel is a sustainable energy substitute with environmentally friendly characteristics. Immobilized-enzyme technology improves the stability, reactivity, and reusability of biodiesel production. The potential and importance of immobilized-enzyme technology in biodiesel production are significant.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2022)
Article
Environmental Sciences
Sathish Kumar Rajamanickam, Sureshkumar Kasinathan
Summary: This study successfully produced renewable biofuel from Manilkara zapota fruit and seed oil, with bioethanol and ethyl ester displaying potential as substitutes for fossil diesel and gasoline. Optimal production parameters were determined through experimentation, highlighting the promise of these biofuels as sustainable alternatives.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Physical
Le Zhong, Yuxiao Feng, Hongtong Hu, Jiabao Xu, Ziyuan Wang, Yingjie Du, Jiandong Cui, Shiru Jia
Summary: In this study, the hydrophobic ZIF-L coated with PDMS was successfully used to immobilize lipase from Aspergillus oryzae, enhancing its stability and activity recovery. The immobilized lipase exhibited better storage stability and catalyzed higher biodiesel yield, showing great potential for biodiesel production.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Energy & Fuels
Jun Zhang, Xiaoyan Chen, Pengmei Lv, Wen Luo, Zhiyuan Wang, Jingliang Xu, Zhongming Wang
Summary: In this study, a green and simple bionic-immobilization method was developed for the immobilization of recombinant lipase using bio-silicification, resulting in a high immobilization efficiency and activity recovery. The bionic-immobilization significantly improved the thermal stability, storage stability, and resistance of the bionic-immobilized lipase, with key amino acid residues and molecular conformation playing crucial roles. Additionally, biodiesel production using the bionic-immobilized lipase as catalyst showed high yield and catalytic activity, demonstrating the potential of bionic-immobilization for industrial applications.
Article
Energy & Fuels
Ehsan Parandi, Maryam Safaripour, Magda H. Abdellattif, Majid Saidi, Alireza Bozorgian, Hamid Rashidi Nodeh, Shahabaldin Rezania
Summary: The main objective of this study was to produce biodiesel from waste cooking oil using immobilized lipase on a magnetic nanocomposite. The synthesized biocatalyst showed high efficiency in producing biodiesel at lower temperatures and optimal reaction conditions.
Article
Green & Sustainable Science & Technology
Rahmath Abdulla, Eryati Derman, Thivyasri K. Mathialagan, Abu Zahrim Yaser, Mohd Armi Abu Samah, Jualang Azlan Gansau, Syed Umar Faruq Syed Najmuddin
Summary: This study optimized the transesterification process to improve the efficiency of biodiesel production using WPCO. The results demonstrated that WPCO can be a potential feedstock for biodiesel production with the use of environmentally friendly biocatalysts like immobilized lipase.
Article
Green & Sustainable Science & Technology
Luciana da Silva Dutra, Martina Costa Cerqueira Pinto, Eliane Pereira Cipolatti, Erika Cristina G. Aguieiras, Evelin Andrade Manoel, Jaqueline Greco-Duarte, Denise Maria Guimaraes Freire, Jose Carlos Pinto
Summary: The study conducted an in-depth analysis of global scientific production on biodiesel production through immobilized enzymes, revealing widespread international collaboration and interest in this field, with China, Brazil, and India emerging as key countries in research and development.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Su Yeon Choi, Wooil Choi, Yoon Shin Park, Hye Kwon Kim, Yang-Hoon Kim, Jiho Min
Summary: The aim of this study is to determine the effects of yeast vacuoles on triglyceride accumulation and differentiation of pre-adipocytes to adipocytes using 3T3-L1 cells. After treatment with vacuoles, lipid droplets and triglyceride accumulation were reduced, and expression levels of C/EBP alpha and PPAR gamma in vacuole-treated adipocytes were significantly reduced. Yeast-derived vacuoles could be an important source for inhibiting lipid accumulation and obesity-related inflammation by suppressing adipogenesis.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Xiyue Cao, Hui Xu, Fosheng Li, Yulu Ran, Xiaorui Ma, Yu Cao, Qingrui Xu, Dairong Qiao, Yi Cao
Summary: A method for direct transesterification of wet yeast using immobilized lipase was developed in this study, which improved the efficiency of biodiesel production. The immobilized lipase covalently cross-linked with Fe3O4@SiO2-CHO showed high enzyme activity and immobilization rate. The optimal temperature for the immobilized lipase was 60 degrees C, and it still retained 80% activity after storage.
Article
Engineering, Multidisciplinary
Rana Abbas Azeez, Firas Khaleel Ismael Al-Zuhairi
Summary: Bioremediation is an efficient technique for environmental pollution control. In this study, yeast cells were immobilized on the surface of magnetic nanoparticles to remove dye from aqueous solution. The experiments determined that the optimal conditions for removal efficiency were pH 6.5, 50 mg/l dye concentration, 1.5 g/l biosorbent dosage, 110 rpm agitation, and 35 degrees C temperature. The adsorption isotherm analysis indicated that the biosorption process followed the Freundlich model, and it was exothermic and spontaneous.
ALEXANDRIA ENGINEERING JOURNAL
(2022)
Article
Thermodynamics
Archana Dash, Rintu Banerjee
Summary: In this study, lipase was produced using Solid State Fermentation and immobilized on activated celite for transesterification, resulting in high fatty acid methyl ester (FAME) conversions under optimized conditions. The spent lipase showed potential for cyclic use, maintaining almost 90% residual activity after multiple cycles.
Article
Chemistry, Physical
Azhar A. Najjar, Elhagag A. Hassan, Nidal M. Zabermawi, Saad B. Almasaudi, Mohammed Moulay, Steve Harakeh, Mohamed Abd El-Aal
Summary: This study successfully converted cooking oil wastes into biodiesel using immobilized lipase on Fe3O4/cellulose nanocomposite, demonstrating improved stability and reusability of the catalyst. The produced biodiesel met the standards and can be utilized as diesel fuel in daily life.
Article
Environmental Sciences
Wannapawn Watsuntorn, Nuttha Chuengcharoenphanich, Poompat Niltaya, Cheryanus Butkumchote, Monnat Theerachat, Chompunuch Glinwong, Wei Qi, Zhongming Wang, Warawut Chulalaksananukul
Summary: The study identified a novel Saccharomyces cerevisiae CU-TPD4 isolate from coconut waste residues in Thailand, which was confirmed to be oleaginous yeast with high lipid content. Through optimized growth conditions, the lipid yield of CU-TPD4 was significantly increased, making it a promising candidate for biodiesel production.
Article
Biochemistry & Molecular Biology
Abir Ben Bacha, Mona Alonazi, Mona G. Alharbi, Habib Horchani, Imen Ben Abdelmalek
Summary: This study successfully produced biodiesel with high yield using waste cooking oil through enzymatic transesterification reaction, and employed immobilized lipases as environmentally friendly catalysts, which exhibited superior stability and activity compared to free lipases. The produced biodiesel complied with international specifications and possessed desirable properties.
Article
Biochemistry & Molecular Biology
Kunio Nakatsukasa, Munetaka Fujisawa, Xiaotan Yang, Tomoyuki Kawarasaki, Fumihiko Okumura, Takumi Kamura
Summary: This study found that Tgl4 is a stable protein that can be inactivated through dephosphorylation, playing an important regulatory role in the cell cycle.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
