Review
Oncology
Kunishige Onuma, Masahiro Inoue
Summary: Apico-basal polarity in epithelial cells is a fundamental property that plays a critical role in cell connection, barrier function, and direction determination. Loss of polarity is associated with cancer progression, while dysregulated polarity is observed in differentiated carcinomas.
Article
Cell Biology
Tingting Cai, Juan Peng, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama-Diagne
Summary: The cytoskeleton plays a crucial role in cellular polarity, and septins are a family of GTPases that bind to membranes and recruit cytoskeletal elements to perform their biological functions. However, the role of septins in apico-basal polarity and their membrane-binding ability have not been well investigated.
Article
Biology
Marina Goncalves Antunes, Matthieu Sanial, Vincent Contremoulins, Sandra Carvalho, Anne Plessis, Isabelle Becam
Summary: This study reveals how the hedgehog (HH) morphogen regulates the subcellular localization and activity of the oncogenic G-protein-coupled receptor (GPCR) Smoothened (SMO), thereby affecting the morphogenesis of epithelial structures. The authors provide evidence that HH promotes the recycling of internalized SMO, leading to its stabilization. This effect relies on the action of protein kinase A, casein kinase I, and Fused (FU) kinase. Additionally, at high HH levels, FU leads to the local enrichment of SMO in the basal domain of the cell membrane.
Article
Biochemistry & Molecular Biology
Mayumi Mori, Julie Ruer-Laventie, Wandrille Duchemin, Philippe Demougin, Tohnyui Ndinyanka Fabrice, Matthias P. Wymann, Jean Pieters
Summary: Coronin 1 protein plays a critical role in the survival of naive T cells in the periphery by maintaining the activity of PI3K delta and suppressing apoptosis. Furthermore, it functions independently of pMHC:TCR and IL-7 signaling, but is essential for peripheral T cell survival.
Article
Pediatrics
Jing Yin, Jijun Ma, Jingyue Xia, Yang Cao, Chongwei Li
Summary: This study reports a case of APDS1 with a unique clinical phenotype presenting as SLE and SS-like features. The patient achieved remission after treatment with medication.
FRONTIERS IN PEDIATRICS
(2022)
Article
Biochemistry & Molecular Biology
Aneta Manda-Handzlik, Agnieszka Mroczek, Weronika Kuzmicka, Adrianna Cieloch, Zuzanna Homoncik, Angelika Muchowicz, Urszula Demkow, Malgorzata Wachowska
Summary: The aim of this study was to investigate the role of p110 delta in neutrophil antimicrobial functions, activation status, and cytokine production. The results showed that p110 delta is not a key player in most neutrophil functions in mice. Further research is recommended to correlate these in vitro results with in vivo observations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Fernanda Scopelliti, Laura Mercurio, Caterina Cattani, Valentina Dimartino, Cristina Albanesi, Gianfranco Costanzo, Concetta Mirisola, Stefania Madonna, Andrea Cavani
Summary: PI3K p110 delta plays a crucial role in regulating the functions of human dendritic cells, and selective inhibition with seletalisib can reduce the maturation and antigen-presenting functions of DCs, leading to a decreased immune response and increased cell migration.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Hematology
Matthew S. Davids, Owen A. O'Connor, Wojciech Jurczak, Felipe Samaniego, Timothy S. Fenske, Pier Luigi Zinzani, Manish R. Patel, Nilanjan Ghosh, Bruce D. Cheson, Enrico Derenzini, Danielle M. Brander, James A. Reeves, Wanda Knopinska-Posluszny, John N. Allan, Tycel Phillips, Paolo F. Caimi, Ewa Lech-Maranda, John M. Burke, Richy Agajanian, Ruth Pettengell, Lori A. Leslie, Chan Y. Cheah, Gustavo Fonseca, James Essell, Julio C. Chavez, John M. Pagel, Jeff P. Sharman, Yanzhi Hsu, Hari P. Miskin, Peter Sportelli, Michael S. Weiss, Ian W. Flinn
Summary: Umbralisib, a dual inhibitor of PI3K delta and CK1 epsilon, showed favorable long-term tolerability in the treatment of lymphoid malignancies. Common adverse events include diarrhea, nausea, and fatigue, with low rates of serious adverse events of special interest.
Article
Immunology
Anna Cutrina-Pons, Aloka De Sa, David J. Fear, Hannah J. Gould, Faruk Ramadani
Summary: Phosphoinositide 3-kinase (PI3K) p110d signaling negatively regulates the production of mouse IgE. However, its role in the production of human IgE is yet to be determined. In this study, the effect of PI3K p110d inhibition on human IgE production was investigated using stimulated human B cells. It was found that PI3K p110d inhibition significantly reduced IgE production and affected the differentiation of plasma cells.
Article
Medicine, Research & Experimental
Lijun Dong, Haote Han, Xionggao Huang, Gaoen Ma, Dong Fang, Hui Qi, Zhuo Han, Luping Wang, Jingkui Tian, Bart Vanhaesebroeck, Guoming Zhang, Shaochong Zhang, Hetian Lei
Summary: The specific inhibitor Idelalisib has shown potential in preventing proliferative vitroretinopathy (PVR) by inhibiting Akt activation, fibronectin expression, and collagen gel contraction in human RPE cells and an animal model.
LABORATORY INVESTIGATION
(2022)
Article
Multidisciplinary Sciences
Keyur Thummar, Chozha Vendan Rathinam
Summary: This study identifies the crucial role of the regulatory subunits p85 alpha and p85 beta in the differentiation and distribution of dendritic cells (DCs). Deficiency of p85 leads to increased differentiation of specific DC subsets in the spleen, while decreasing the numbers of DCs in the bone marrow, thymus, and lymph nodes. Molecular studies reveal altered signal transduction pathways in p85 mutant DC subsets, indicating the importance of p85 in DC development and maintenance.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Ghislain Gillard, Gemma Girdler, Katja Roper
Summary: This study reveals the mechanism by which non-centrosomal microtubule arrays are crucial for tube formation in Drosophila salivary glands, involving Katanin-mediated release of microtubules, Patronin recruitment, and array formation.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Qing Wang, Tian Xie, Zhonghua Ning, Cong Chen, Ying Man, Baoshan Cui
Summary: Enhancing lateral hydrological connectivity in river systems can increase material and energy exchange, improve species diversity, and benefit plant re-establishment in bare areas. This study explored the effects of enhancing lateral hydrological connectivity on plant life history process and found that it significantly improved seed retention, emergence, and seedling survival.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Sicong Wang, Chien-Wei Lin, Amber E. Carleton, Chari L. Cortez, Craig Johnson, Linnea E. Taniguchi, Nikola Sekulovski, Ryan F. Townshend, Venkatesha Basrur, Alexey Nesvizhskii, Peng Zou, Jianping Fu, Deborah L. Gumucio, Mara C. Duncan, Kenichiro Taniguchi
Summary: Critical early steps in human embryonic development involve the polarization of the inner cell mass, followed by the formation of an expanded lumen called the epiblast cavity. Recent studies have shown that 3D human pluripotent stem cell-derived cyst structures can replicate these processes. Using APEX2-based proximity biotinylation and quantitative mass spectrometry, researchers identified previously unannotated proteins in apical and basolateral membrane territories of these cysts. Functional experiments confirmed the importance of apically enriched proteins in cyst morphogenesis, highlighting the role of AP-1 clathrin adaptor complex in expanding apical membrane domains during the formation of the lumen. This study demonstrates the effectiveness of proximity labeling in discovering novel regulators of epithelial morphogenesis in 3D stem cell-based models.
Article
Biochemistry & Molecular Biology
Marzia Di Donato, Pia Giovannelli, Antimo Migliaccio, Antonio Bilancio
Summary: Breast cancer, the most common cancer worldwide, remains the leading cause of cancer-related deaths despite advancements in early detection and treatment. This study explores the efficacy of PI3K-selective inhibitors in reducing migration and proliferation of breast cancer cells, suggesting their potential as therapeutic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Wan J. Gan, Michael Zavortink, Christine Ludick, Rachel Templin, Robyn Webb, Richard Webb, Wei Ma, Philip Poronnik, Robert G. Parton, Herbert Y. Gaisano, Annette M. Shewan, Peter Thorn
JOURNAL OF CELL SCIENCE
(2017)
Correction
Biochemistry & Molecular Biology
Sang-Ho Kwon, Sekyung Oh, Marisa Nacke, Keith E. Mostov, Joshua H. Lipschutz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Multidisciplinary Sciences
Alvaro Roman-Fernandez, Julie Roignot, Emma Sandilands, Marisa Nacke, Mohammed A. Mansour, Lynn McGarry, Emma Shanks, Keith E. Mostov, David M. Bryant
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Wei Yu, Wallace F. Marshall, Ross J. Metzger, Paul R. Brakeman, Leonardo Morsut, Wendell Lim, Keith E. Mostov
Review
Cell Biology
Jing Yang, Parker Antin, Geert Berx, Cedric Blanpain, Thomas Brabletz, Marianne Bronner, Kyra Campbell, Amparo Cano, Jordi Casanova, Gerhard Christofori, Shoukat Dedhar, Rik Derynck, Heide L. Ford, Jonas Fuxe, Antonio Garcia de Herreros, Gregory J. Goodall, Anna-Katerina Hadjantonakis, Ruby J. Y. Huang, Chaya Kalcheim, Raghu Kalluri, Yibin Kang, Yeesim Khew-Goodall, Herbert Levine, Jinsong Liu, Gregory D. Longmore, Sendurai A. Mani, Joan Massague, Roberto Mayor, David McClay, Keith E. Mostov, Donald F. Newgreen, M. Angela Nieto, Alain Puisieux, Raymond Runyan, Pierre Savagner, Ben Stanger, Marc P. Stemmler, Yoshiko Takahashi, Masatoshi Takeichi, Eric Theveneau, Jean Paul Thiery, Erik W. Thompson, Robert A. Weinberg, Elizabeth D. Williams, Jianhua Xing, Binhua P. Zhou, Guojun Sheng
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Tianfang Wang, Sang-Ho Kwon, Xiao Peng, Severine Urdy, Zefu Lu, Robert J. Schmitz, Stephen Dalton, Keith E. Mostov, Shaying Zhao
Article
Pathology
Ankur Pandita, Matias Ekstrand, Sara Bjursten, Zhiyuan Zhao, Per Fogelstrand, Kristell Le Gal, Lars Ny, Martin O. Bergo, Joakim Karlsson, Jonas A. Nilsson, Levent M. Akyurek, Malin C. Levin, Jan Boren, Andrew J. Ewald, Keith E. Mostov, Max Levin
Summary: Intussusceptive angiogenesis, characterized by intravascular pillars, may play a role in the growth of human melanoma metastases. Inhibition of matrix metalloproteinase 9 (MMP9) could potentially block pillar formation and serve as a promising anti-angiogenic drug target for metastatic melanoma.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jonas Honer, Katie M. Niemeyer, Christian Fercher, Ana L. Diez Tissera, Noushin Jaberolansar, Yohaann M. A. Jafrani, Chun Zhou, Julio J. Caramelo, Annette M. Shewan, Benjamin L. Schulz, Jeffrey L. Brodsky, Lucia F. Zacchi
Summary: This study investigated the impact of ER redox homeostasis on post-translational modifications (PTMs) and protein biogenesis using torsinA as a model substrate. It highlighted the role of a specific protein disulfide isomerase, Pdi1, in affecting the folding and N-linked glycosylation of torsinA and torsinA Delta E, providing insights into the sensitive nature of early torsinA folding intermediates to perturbed interactions between Cys residues and the quality control machinery.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Correction
Cell Biology
Jing Yang, Parker Antin, Geert Berx, Cedric Blanpain, Thomas Brabletz, Marianne Bronner, Kyra Campbell, Amparo Cano, Jordi Casanova, Gerhard Christofori, Shoukat Dedhar, Rik Derynck, Heide L. Ford, Jonas Fuxe, Antonio Garcia de Herreros, Gregory J. Goodall, Anna-Katerina Hadjantonakis, Ruby Y. J. Huang, Chaya Kalcheim, Raghu Kalluri, Yibin Kang, Yeesim Khew-Goodall, Herbert Levine, Jinsong Liu, Gregory D. Longmore, Sendurai A. Mani, Joan Massague, Roberto Mayor, David McClay, Keith E. Mostov, Donald F. Newgreen, M. Angela Nieto, Alain Puisieux, Raymond Runyan, Pierre Savagner, Ben Stanger, Marc P. Stemmler, Yoshiko Takahashi, Masatoshi Takeichi, Eric Theveneau, Jean Paul Thiery, Erik W. Thompson, Robert A. Weinberg, Elizabeth D. Williams, Jianhua Xing, Binhua P. Zhou, Guojun Sheng
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ying Yang, Pekka Paivinen, Chang Xie, Alexis Leigh Krup, Tomi P. Makela, Keith E. Mostov, Jeremy F. Reiter
Summary: The study shows that ciliary Hedgehog signaling patterns the circumferential smooth muscle to generate radial mechanical forces that activate YAP and elongate the gut.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Pavel Nedvetsky, Ivo Cornelissen, Thomas Mathivet, Claire Bouleti, Phalla Ou, Pieter Baatsen, Xiaocheng Zhao, Frans Schuit, Fabio Stanchi, Keith E. Mostov, Holger Gerhardt
Summary: Endothelial cAMP-dependent protein kinase A (PKA) plays a crucial role in vascular development and homeostasis. Inhibition of PKA in endothelial cells leads to excessive angiogenesis and severe systemic effects, including edema, hypoalbuminemia, hypoglycemia and premature death. Specifically, PKA inhibition causes disorganization of liver vasculature and abnormal angiogenic response in multiple tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Meeting Abstract
Cell Biology
A. Roman-Fernandez, J. Roignot, E. Sandilands, M. Nacke, M. Mansour, L. McGarry, E. Shanks, K. E. Mostov, D. M. Bryant
MOLECULAR BIOLOGY OF THE CELL
(2018)
Meeting Abstract
Cell Biology
Y. Yang, P. Paivinen, K. E. Mostov, T. P. Makela, J. F. Reiter
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Developmental Biology
Lei Gao, Zhufeng Yang, Chitkale Hiremath, Susan E. Zimmerman, Blake Long, Paul R. Brakeman, Keith E. Mostov, David M. Bryant, Katherine Luby-Phelps, Denise K. Marciano
Article
Cell Biology
Anirban Datta, Emma Sandilands, Keith E. Mostov, David M. Bryant
CELLULAR SIGNALLING
(2017)