Journal
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
Volume 281, Issue 1795, Pages -Publisher
ROYAL SOC
DOI: 10.1098/rspb.2014.1422
Keywords
ammonia excretion; sodium uptake; oxygen consumption; gill development; rainbow trout (Oncorhynchus mykiss) larvae; ionoregulatory hypothesis
Categories
Funding
- NSERC (Natural Sciences and Engineering Research Council of Canada) Discovery grants
- NSERC Canada Graduate Scholarship
- Canada Research Chair Program
Ask authors/readers for more resources
Post-hatch fishes lack a functional gill and use cutaneous surfaces for exchange with the surrounding environment. The ionoregulatory hypothesis posits that ionoregulation is the first physiological process to be limited by cutaneous exchange, necessitating its shift to the gills. We hypothesized that the ontogeny of branchial ammonia excretion (J(amm)) is coupled to Na+ uptake (J(in)(Na)) in accordance with the current model for Na+= NH4+ exchange in freshwater. Using divided chambers, branchial and cutaneous J(amm), J(in)(Na) and oxygen consumption (MO2) by larval rainbow trout were assessed. Following hatch, the skin accounted for 97% and 86% of total Jamm and JNa in, respectively. J(amm) and JNa in shifted to the gills simultaneously at 15 days post-hatch (dph) and were highly correlated (R-2 = 0.951) at the gills, but not the skin, over development. Contrastingly, MO2 shifted significantly later at 27 dph, in agreement with the ionoregulatory hypothesis. Moreover, the mRNA expression and/ or enzymatic activity of Rhesus proteins, Na+/H+-exchanger, H+-ATPase, Na+/K+-ATPase and carbonic anhydrase, all key components of the Na+= NH4+-exchange system, increased in the gills over larval development. We propose that the ontogeny of branchial JNa in occurs as Na+= NH4+ exchange and provide evidence for a novel element to the ionoregulatory hypothesis, the excretion of potentially lethal metabolic ammonia.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available