Journal
ONCOLOGY LETTERS
Volume 9, Issue 3, Pages 1207-1212Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2015.2877
Keywords
circulating miRNA; breast cancer; let-7c; predictive factor; receiver operating characteristic analysis
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Funding
- National Natural Science Foundation [31371321, 31440061, 81200601]
- Taishan Scholars program of Shandong Province
- Shandong Science and Technology Committee [ZR2012HQ035]
- Foundation of Shandong Educational Committee [J11LC01]
- [NCET-10-0919]
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Circulating microRNAs (miRNAs) are important in the diagnosis of a number of diseases, since serum or plasma miRNAs are more stable compared with miRNA isolated from blood samples. The aim of the present study was to investigate the association between the expression levels of serum let-7c miRNA and the clinical diagnosis of breast cancer (BC). The circulating let-7c levels of 90 BC patients and 64 healthy controls were determined by performing a reverse transcription-quantitative polymerase chain reaction assay. The results demonstrated that let-7c expression was downregulated in the BC tissues compared with the paracarcinoma control tissues. In addition, the let-7c expression in the serum of BC patients was significantly lower compared with the healthy controls (P<0.01). Using a cutoff value of 0.374x10(3) copies/ml, the serum expression levels of let-7c exhibited 87.5% sensitivity and 78.9% specificity for distinguishing BC patients from healthy controls (area under the receiver operating characteristic curve, 0.848; 95% confidence interval, 0.785-0.911). Furthermore, the results demonstrated that the serum expression levels of let-7c were significantly higher in premenopausal compared with postmenopausal patients (P<0.05), supporting the hypothesis that postmenopausal status may affect the serum expression levels of let-7c. However, no statistically significant differences were detected in the serum levels of let-7c between ER (or PR)-positive and -negative patients. Therefore, the current study hypothesized that serum let-7c may be used as a novel and valuable biomarker for the diagnosis of BC.
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