Article
Biochemistry & Molecular Biology
Gisela Altes, Marta Vaquero, Sara Cuesta, Carlos Anerillas, Anna Macia, Carme Espinet, Joan Ribera, Saverio Bellusci, Ophir D. Klein, Andree Yeramian, Xavi Dolcet, Joaquim Egea, Mario Encinas
Summary: The Wolffian ducts (WD) play a crucial role in the development of the mammalian genitourinary tract. This study reveals that mutation of tyrosine 53 of Spry1 generates a dominant negative allele that leads to developmental defects in the caudal portions of the WD. These defects include ectopically branched seminal vesicles in males and persistent WD in females. The study also shows that these defects can be rescued by lowering Fgf10 levels.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pedro L. R. Guedes, Carolina P. F. Carvalho, Adriana A. F. Carbonel, Manuel J. Simoes, Marcelo Y. Icimoto, Jair A. K. Aguiar, Maria Kouyoumdjian, Marcos L. Gazarini, Marcia R. Nagaoka
Summary: Chondroitin sulfate (CS) has been found to have therapeutic effects in an experimental model of bile duct ligation. CS reduces collagen content and increases liver parenchyma content, and can alter the hepatic collagen III/I ratio. Additionally, CS reduces cell death indicators and promotes regeneration. Therefore, CS has potential hepatoprotective action in fibrogenesis induced by bile duct ligation.
Article
Biochemistry & Molecular Biology
Qiting Zhang, Ziyan Wang, Xinyuan Chen, Haoxiang Qiu, Yifan Gu, Ning Wang, Tao Wang, Ze Wang, Huabin Ma, Yufen Zhao, Bin Zhang
Summary: The compound 8a acts as an HDAC1 inhibitor with anti-proliferative and pro-apoptotic activities against leukemia cells, mainly entering the nucleus and inducing cell apoptosis through the mitochondrial pathway. It also affects the expression of DNMT1, leading to inhibition of proliferation and promotion of apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Maira Bianchi Rodrigues Alves, Laura Girardet, Celine Augiere, Kyeong Hye Moon, Camille Lavoie-Ouellet, Agathe Bernet, Denis Soulet, Ezequiel Calvo, Maria E. Teves, Charles Joly Beauparlant, Arnaud Droit, Alexandre Bastien, Claude Robert, Jinwoong Bok, Barry T. Hinton, Clemence Belleannee
Summary: Primary cilia are crucial for embryonic patterning and organogenesis through the Hedgehog signaling pathway. This study investigated the role of Hedgehog signaling and primary cilia in the development of male reproductive ductules, such as the epididymis. The findings suggest that primary cilia-dependent Hedgehog signaling acts as a master regulator of genes involved in Wolffian duct development, providing new insights into sexual differentiation and male infertility issues. Modulating primary ciliogenesis and downstream Hedgehog signaling may control the development of the Wolffian duct.
BIOLOGY OF REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Dongxing Shao, Cihang Liu, Yingying Wang, Jing Lin, Xiaolei Cheng, Pei Han, Zhen Li, Dongdong Jian, Junwei Nie, Mingyang Jiang, Yuanzhi Wei, Junyue Xing, Zhiping Guo, Wengong Wang, Xia Yi, Hao Tang
Summary: This study reveals the interplay between DNA methylation by DNMT1 and mRNA methylation by NSun2 in regulating osteosarcoma cell apoptosis. DNMT1 induces promoter methylation of NSun2, resulting in its suppressed expression. Additionally, DNMT1 and NSun2 regulate the expression of anti-apoptotic genes AXL, NOTCH2, and YAP1 through DNA and mRNA methylation. The findings highlight the importance of DNA and RNA cytosine methylations in determining osteosarcoma resistance to apoptosis during chemotherapy.
Article
Biochemistry & Molecular Biology
Hiroyuki Iio, Tadahiko Kikugawa, Yuichiro Sawada, Hiroshi Sakai, Shuhei Yoshida, Yuta Yanagihara, Aoi Ikedo, Noritaka Saeki, So-ichiro Fukada, Takashi Saika, Yuuki Imai
Summary: Dnmt1 plays a crucial role in skeletal muscle regeneration, as indicated by the significant decrease in muscle weight and cell number in Dnmt1 knockout mice. RNA sequencing analysis revealed alterations in gene expression related to cell adhesion and apoptosis in satellite cells from knockout mice.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Yuting Tang, Fangling Hong, Siyang Ding, Jiashu Yang, Ming Zhang, Yunfei Ma, Que Zheng, Dawei Yang, Yucui Jin, Changyan Ma
Summary: In this study, it was found that the upregulation of long noncoding RNA (lncRNA) IGFBP7-OT and its maternal gene, IGFBP7, in osteoarthritic cartilage were positively correlated. Overexpression of IGFBP7-OT inhibited chondrocyte viability, promoted chondrocyte apoptosis, and reduced extracellular matrix components, while knockdown of IGFBP7-OT had the opposite effects. IGFBP7-OT overexpression promoted cartilage degeneration and aggravated the monosodium iodoacetate-induced osteoarthritis phenotype in vivo. Mechanistic research revealed that IGFBP7-OT promoted osteoarthritis progression by upregulating IGFBP7 expression through inhibiting methylation of the IGFBP7 promoter by suppressing the occupancy of DNMT1 and DNMT3a. The upregulation of IGFBP7-OT in osteoarthritis was partially controlled by METTL3-mediated N6-methyladenosine (m6A) modification.
Article
Biochemistry & Molecular Biology
Longxiang Wu, Zhongqing Yang, Guoyu Dai, Benyi Fan, Junbin Yuan, Yalin Liu, Peihua Liu, Zhenyu Ou
Summary: This study reveals the increased expression of SOX5 in bladder cancer and its role in promoting cell growth and migration. It also shows that suppressing SOX5 inhibits the expression of DNMT1 and overexpressing DNMT1 alleviates the cell progress of bladder cancer inhibited by SOX5. The study suggests that SOX5 exerts its functions in bladder cancer cells by modulating the SOX5/DNMT1/p21 pathway.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Biology
Ying Zhu, Xinru Wang, Xiaoyun Zhou, Lexi Ding, Dan Liu, Huizhuo Xu
Summary: The study found that DNMT1-mediated PPAR alpha methylation promotes apoptosis and ROS levels in HRCPs, exacerbating retinal tissue damage in DR mice.
BIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ning Ding, Lin Xie, Fei Ma, Shengchao Ma, Jiantuan Xiong, Guanjun Lu, Huiping Zhang, Yideng Jiang
Summary: Elevated homocysteine (Hcy) levels were found to downregulate the expression of miR-30a-5p in glomerular podocytes, leading to podocyte apoptosis. miR-30a-5p was shown to directly target the FOXA1 gene and inhibit its expression. Additionally, DNA methylation of the miR-30a-5p promoter was observed, with DNA methyltransferase enzyme 1 (DNMT1) identified as the key regulator.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Endocrinology & Metabolism
Caixia Li, Bing Chen, Hai Yu, Yonggang Zhang
Summary: This study aims to investigate the role of DNM3OS in regulating the growth of hepatocellular carcinoma (HCC) cells through the DNMT1/FOXO3a pathway. LncRNAs related to liver cancer were identified through the LncRNADisease v2.0 database. DNM3OS was found to promote methylation of the FOXO3a promoter region and inhibit FOXO3a transcription, thereby acting as an oncogene in liver cancer progression. The findings provide new insights into the molecular mechanisms underlying HCC.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Oncology
Reiko Doi, Yuki Fukumura, Rong Lu, Kenichi Hirabayashi, Yuko Kinowaki, Yasuni Nakanuma, Yoshiakira Kanai, Jun Nakahodo, Noriko Sasahara, Tsuyoshi Saito, Takashi Yao
Summary: This study identified differences between type 1 and type 2 intraductal papillary neoplasms of the bile duct (IPNB) using multiple gene expression analysis and immunohistochemistry. Type 2 IPNB showed increased expression of DNMT1 protein and DNA methylation frequency compared to type 1. DNMT1 IHC may be useful in distinguishing between these two types.
ANTICANCER RESEARCH
(2022)
Article
Materials Science, Multidisciplinary
Ying Wang, Jiasheng Chen, Meng Duan, Weixin Zhao, Hui Cheng, Ming Yang, Meng Liu, Jianwen Huang, Guo Gao, Qiang Fu
Summary: In this study, a biomimetic proangiogenic scaffold was developed by modifying the ECM bioscaffolds with silk fibroin (SF) to improve mechanical properties and conjugated superparamagnetic iron oxide nanoparticles (Fe3O4 NP-VEGF) to promote angiogenesis. The proangiogenic scaffold supported cell adhesion and proliferation in vitro and significantly accelerated vascularization in a rat bladder augmentation model, leading to urothelium and smooth muscle regeneration.
NPG ASIA MATERIALS
(2022)
Article
Food Science & Technology
Shisan Xu, Huan Zhang, Cun-Zhao Li, Ping-Shan Lai, Guijiang Wang, Yu Suen Chan, Shuk Han Cheng, Xueping Chen
Summary: CBD extract accelerates fin regeneration and reduces apoptosis after amputation by modulating the inflammatory response and the IL-1 beta/Caspase 3/PARP signal pathway.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Surgery
Smita Khetarpal, Anil Khetarpal
Summary: During embryogenesis, hormonal influence causes the Mullerian duct system to develop into the female genital tract, while the Wolffian duct system may regress. Gartner's cysts, arising from remnants of the Wolffian duct, are rare and mostly asymptomatic, but can sometimes cause symptoms like dyspareunia and voiding disturbances.
INDIAN JOURNAL OF SURGERY
(2022)
Article
Multidisciplinary Sciences
Samuel Thomas, Christopher D. Dunn, Lewis J. Campbell, Douglas W. Strand, Chad M. Vezina, Dale E. Bjorling, Kristina L. Penniston, Lingjun Li, William A. Ricke, Tony L. Goldberg
Summary: There was a significant association found between the presence of JC virus in urine and a diagnosis of LUTS. Urine metabolomics analysis identified dysregulation of metabolites associated with key LUTS processes.
Article
Developmental Biology
Diya B. Joseph, Anne E. Turco, Chad M. Vezina, Douglas W. Strand
Summary: The prostate develops through epithelial budding and branching processes during fetal and postnatal stages, showing remarkable regenerative capacity. Benign prostatic hyperplasia (BPH) and prostate cancer (PCa) may involve androgen-independent epithelial progenitors. Multiple castration-insensitive epithelial cell types exist in the proximal area of the prostate, with not all acting as progenitors.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Karen K. Deal, Anoop S. Chandrashekar, M. Makenzie Beaman, Meagan C. Branch, Dennis P. Buehler, Simon J. Conway, E. Michelle Southard-Smith
Summary: Mouse models of Spina bifida have been useful in identifying genes, developmental processes, and environmental factors impacting neurulation and neural tube closure. The Pax3Sp-d mouse model shows altered bladder innervation in SB, which may provide insights into the developmental origins of bladder dysfunction in SB patients.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Anne E. Turco, Steven R. Oakes, Kimberly P. Keil Stietz, Cheryl L. Dunham, Diya B. Joseph, Thrishna S. Chathurvedula, Nicholas M. Girardi, Andrew J. Schneider, Joseph Gawdzik, Celeste M. Sheftel, Peiqing Wang, Zunyi Wang, Dale E. Bjorling, William A. Ricke, Weiping Tang, Laura L. Hernandez, Janet R. Keast, Adrian D. Bonev, Matthew D. Grimes, Douglas W. Strand, Nathan R. Tykocki, Robyn L. Tanguay, Richard E. Peterson, Chad M. Vezina
Summary: This study identified the origin of male LUTD in the intrauterine environment of developing males, showing that exposure to environmental contaminant TCDD during pregnancy can lead to hyperinnervation of the prostate and neuromuscular dysfunction in adulthood.
DISEASE MODELS & MECHANISMS
(2021)
Article
Oncology
Diya B. Joseph, Gervaise H. Henry, Alicia Malewska, Jeffrey C. Reese, Ryan J. Mauck, Jeffrey C. Gahan, Ryan C. Hutchinson, Venkat S. Malladi, Claus G. Roehrborn, Chad M. Vezina, Douglas W. Strand
Summary: Through single-cell RNA sequencing, different stromal cell types in the human prostate were identified, including three smooth muscle subtypes and two fibroblast subtypes. The research provides insight into the molecular identity and anatomy of discrete stromal cell types in the prostate, laying the foundation for new therapeutic targets in benign prostatic hyperplasia.
JOURNAL OF PATHOLOGY
(2021)
Article
Oncology
Diya B. Joseph, Gervaise H. Henry, Alicia Malewska, Jeffrey C. Reese, Ryan J. Mauck, Jeffrey C. Gahan, Ryan C. Hutchinson, James L. Mohler, Claus G. Roehrborn, Douglas W. Strand
Summary: Benign prostatic hyperplasia (BPH) is a common condition in aging men, treated with 5-alpha reductase inhibitors (SARI) to reduce prostate volume and improve lower urinary tract symptoms. However, the histologic response to SARI treatment is heterogeneous, with potential need for surgical intervention.
JOURNAL OF PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rhonda Bacher, Li-Fang Chu, Cara Argus, Jennifer M. Bolin, Parker Knight, James A. Thomson, Ron Stewart, Christina Kendziorski
Summary: It has been found that equalizing the concentration of cDNA libraries prior to pooling in single-cell RNA-sequencing experiments can improve gene detection rates, enhance biological signals, and reduce technical artifacts. Numerical and in vitro experiments provide evidence to support this finding.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Atul Deshpande, Li-Fang Chu, Ron Stewart, Anthony Gitter
Summary: The algorithm SINGE for gene regulatory network inference from ordered single cell gene expression data outperforms other contemporary algorithms in two mouse embryonic stem cell differentiation datasets, but also reveals caveats about poor performance for individual regulators and uninformative pseudotimes upon more detailed examination.
Review
Developmental Biology
Gregory B. Wiessner, Sakina A. Plumber, Tina Xiang, Cathy L. Mendelsohn
Summary: This Review provides an overview of the characteristics and functions of urothelial cells, including their regenerative capacity during homeostasis and injury repair, as well as their role in tumorigenesis. It also discusses the pathways and signaling mechanisms involved in urothelial differentiation and highlights the relevance of this knowledge to stem cell biology and tissue regeneration.
Article
Biotechnology & Applied Microbiology
Morten Seirup, Srikumar Sengupta, Scott Swanson, Brian E. McIntosh, Mike Collins, Li-Fang Chu, Zhang Cheng, David U. Gorkin, Bret Duffin, Jennifer M. Bolin, Cara Argus, Ron Stewart, James A. Thomson
Summary: In this study, the molecular changes associated with dedifferentiation of hepatocytes in culture were investigated using lineage tracing and comprehensive profiling. The findings revealed three distinct phases of dedifferentiation and drastic changes in chromatin accessibility during the early stages of culture. Furthermore, several candidate transcription factors potentially involved in the dedifferentiation process were identified through motif analysis of open chromatin regions.
Article
Physiology
Celeste M. Sheftel, Luma C. Sartori, Emily R. Hunt, Robbie S. J. Manuel, Autumn M. Bell, Rafael R. Domingues, Lella A. Wake, Brandon R. Scharpf, Chad M. Vezina, Julia F. Charles, Laura L. Hernandez
Summary: This study suggests that peripartum treatment with sertraline may be safer for maternal and offspring bone health compared to fluoxetine. Sertraline reduces maternal calcium concentrations and bone formation markers but does not have significant effects on maternal and offspring bone health.
PHYSIOLOGICAL REPORTS
(2022)
Article
Emergency Medicine
Lifang Chu, Guoying Qian
Summary: With the deepening of the medical and health system, the reform of nursing service model and the provision of holistic nursing care for patients have become the focus. This study found that psychological education intervention among nurses in the department of psychiatry can alleviate professional tiredness, improve psychological elasticity and happiness index, and promote physical and mental health development.
EMERGENCY MEDICINE INTERNATIONAL
(2022)
Article
Cell Biology
Zunyi Wang, Robert Spitz, Chad Vezina, Jianghui Hou, Dale E. Bjorling
Summary: Lower urinary tract symptoms (LUTS) are common among patients with various urological diseases, and incomplete bladder emptying is a typical symptom. The cause of LUTS is largely unknown, but bladder fibrosis seems to play a part in its development. The miR-29 family, known for its anti-fibrotic role, was found to be decreased in bladders of patients and animal models with outlet obstruction, indicating its potential contribution to impaired bladder function. Research on male mice lacking miR-29a/b1 expression showed severe urinary retention, increased voiding duration, reduced flow rate, and abnormal voiding patterns. The increased presence of collagens and elastin in their bladders suggests an important role of miR-29 in bladder homeostasis and its therapeutic potential for improving LUTS symptoms.
DISEASE MODELS & MECHANISMS
(2023)
Meeting Abstract
Ophthalmology
Kimberly L. Edwards, Li-Fang Chu, Yolana C. Martin, James A. Thomson, David M. Gamm
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Su-Tang Lo, Daniel Parrott, M. Veronica Clavijo Jordan, Diya Binoy Joseph, Douglas Strand, U-Ging Lo, Ho Lin, Anza Darehshouri, A. Dean Sherry
Summary: Fluorescent studies demonstrate that ZnT1 is the major zinc efflux transporter in prostate epithelial cells, and loss of ZnT1 via mRNA knockdown combined with lysosomal storage disruption leads to a significant increase in cytosolic zinc. Knockdown of ZnT1 dramatically reduces zinc efflux during glucose-stimulated zinc secretion. Electron microscopy shows that glucose stimulation increases lysosomal storage of zinc, and disruption of lysosomes via Baf or ZnT4 mRNA knockdown results in increased multi-vesicular body formation and cytosolic zinc levels. In human BPH tissue, only the luminal epithelial cells contain significant zinc storage granules.
MOLECULAR IMAGING AND BIOLOGY
(2021)
