Review
Biochemistry & Molecular Biology
H. R. Kaback
Summary: Bacterial cytoplasmic membrane vesicles serve as a unique experimental system for studying active transport, providing a clear definition of the reactions involved in the process. Experimental observations show that respiratory energy in membrane vesicles from Escherichia coli is primarily converted into work in the form of a solute concentration gradient driven by a proton electrochemical gradient, as proposed by Peter Mitchell's chemiosmotic theory.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Article
Biochemistry & Molecular Biology
Chen Wang, Qingjie Xiao, Huaichuan Duan, Jinhong Li, Jiying Zhang, Qisheng Wang, Li Guo, Jianping Hu, Bo Sun, Dong Deng
Summary: Nucleoside homeostasis mediated by transporters and channels is essential for life, and NupG in Escherichia coli serves as a prototype for nucleoside transport. The crystal structures of NupG and its uridine binding site provide a framework for understanding the mechanism of nucleoside transporters in the NHS family.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Jeff Abramson, Ernest M. Wright
Summary: Active transport of sugars into bacteria occurs through symporters driven by ion gradients, with well-studied proton sugar symporter LacY and characterized sodium sugar symporter vSGLT as the main examples. Despite structural differences, they operate through similar mechanisms driven by respective ion electrochemical potential gradients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jiancun Zhao, Yan Liang, Susu Zhang, Zhenshang Xu
Summary: S. thermophilus strains have strain-specific utilization of galactose, and multiple genetic factors can affect their sugar utilization phenotype.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Irena Roterman, Katarzyna Stapor, Leszek Konieczny
Summary: Proteins transporting ions or molecules across the cell membrane play vital roles in maintaining stability and performing biological functions. The hydrophobicity distribution of structures in In and Out conformations affects their stability and conformational changes.
Article
Biochemistry & Molecular Biology
Andre Bazzone, Laura Tesmer, Derya Kurt, H. Ronald Kaback, Klaus Fendler, M. Gregor Madej
Summary: This study used solid-supported membrane-based electrophysiology to investigate the kinetic parameters of sugar translocation by bacterial transporters, and observed real-time sugar binding and transport. It was found that sugar induced conformational changes in the transporters, and the effects of different sugar substrates on conformational states were analyzed. Additionally, the study of mutants revealed the kinetic mechanism of glucose binding and transport.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nina Blaimschein, Parameswaran Hariharan, Selen Manioglu, Lan Guan, Daniel J. Muller
Summary: In this study, single-molecule force spectroscopy was used to investigate the substrate-induced structural changes of MelB from Salmonella typhimurium. The results showed that in the absence of substrate, MelB equally populated two different states, with one state showing higher mechanical structural stability. However, in the presence of melibiose or a coupling Na+-cation, MelB increasingly populated a mechanically less stable state. In the presence of both substrate and co-substrate, this mechanically less stable state of MelB was predominant. These findings provide mechanistic insights into the symport action of galactosides and cations catalyzed by MelB.
Article
Biochemistry & Molecular Biology
Artem Stetsenko, Pavlo Stehantsev, Natalia O. Dranenko, Mikhail S. Gelfand, Albert Guskov
Summary: The study reports the functional and structural analysis of a membrane protein CmaX from a pathogenic Pseudomonas aeruginosa bacterium, which has a signature motif deviating from the canonical one. Despite the difference in composition, CmaX shows no changes in substrate selectivity or transport, suggesting that deviations from the canonical motif can easily alter during evolution.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Sophie Claire de Valk, Robert Mans
Summary: The study successfully altered the substrate specificity of the transport protein Mal11 through laboratory evolution, revealing that three amino acid residue mutations can lead to a disaccharide-insensitive phenotype.
Article
Chemistry, Multidisciplinary
Sandip Chattopadhayay, Anupam Ghosh, Titas Kumar Mukhopadhyay, Rashmi Sharma, Ayan Datta, Pinaki Talukdar
Summary: This study reports a series of self-assembled ion channels with distinctive ion transport ability. The ion transport experiments and electrophysiology experiments confirm their transport activity and selective co-transporting functions. Molecular dynamics simulations reveal the formation of supramolecular channels and the ion selectivity pattern.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Engineering, Chemical
Zhixing Zhao, Bailing Tang, Xiaosheng Yan, Xin Wu, Zhao Li, Philip A. Gale, Yun-Bao Jiang
Summary: Synthetic crown ether-thiourea conjugates can act as a cation/anion symporter with high activity in transporting Na+ and Cl-, but the activity decreases in the presence of high concentrations of K+ ions.
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Janine Lux, Lalaina Holivololona, Raquel San Millan Gutierrez, Markus Hilty, Alban Ramette, Manfred Heller, Lucy J. Hathaway
Summary: Streptococcus pneumoniae is able to sense the presence of Klebsiella pneumoniae peptides in the environment and interacts with them through its substrate binding proteins. This interaction leads to the suppression of pneumococcal growth, with the effect depending on the length of the peptides.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Pornparn Kongpracha, Pattama Wiriyasermkul, Noriyoshi Isozumi, Satomi Moriyama, Yoshikatsu Kanai, Shushi Nagamori
Summary: Membrane proteins play crucial roles in cellular processes, and proteomics is a key method for exploring them. In this study, a membrane enrichment method using urea washing was found to significantly improve the identification of membrane proteins, especially transmembrane proteins.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Plant Sciences
Yazhen Zhang, Kang Wei, Lingling Guo, Yuping Lei, Hao Cheng, Changsong Chen, Liyuan Wang
Summary: This study identified eight members of purine permeases (PUPs) in tea plants and found their significant roles in caffeine metabolism. Further experiments showed that three of these PUPs played a crucial role in caffeine transport and were located in various parts of cells. Functional assays in yeast and Arabidopsis confirmed their roles. This study provides a theoretical basis for further research on PUP genes and new insights into caffeine metabolism in tea plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Marc Teufel, Carlo A. Klein, Maurice Mager, Patrick Sobetzko
Summary: CRISPR SWAPnDROP is a novel genome editing technology that enables large-scale in-vivo DNA transfer between bacterial species. It offers a modular platform and a multi-color scarless co-selection system, facilitating genome editing in various species.
NATURE COMMUNICATIONS
(2022)