Article
Biology
Belen Garcia Pascual, Jan M. Nordbotten, Iain G. Johnston
Summary: Mitochondria and plastids rely on nuclear-encoded genes, but retain small subsets of oDNA. The energetic demands imposed by an organism's changing environment may influence the number of oDNA genes it retains. A mathematical model coupling cell processes and environmental dynamics predicts that species in dynamic environments retain more organelle genes, while those in less dynamic environments retain fewer. This is supported by oDNA data across eukaryotic taxa.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Rotimi Fasimoye, Wentao Dong, Raja S. Nirujogi, Eshaan S. Rawat, Miharu Iguchi, Kwamina Nyame, Toan K. Phung, Enrico Bagnoli, Alan R. Prescott, Dario R. Alessi, Monther Abu-Remaileh
Summary: The Golgi is a crucial organelle involved in protein and lipid biosynthesis, as well as sorting and secretion. Dysfunctional Golgi is associated with various diseases. This study presents a Golgi immunoprecipitation technique to isolate intact Golgi mini-stacks for analysis. The analysis pipeline reveals the molecular features of the human Golgi, including proteome, metabolome, and lipidome profiles. This comprehensive molecular map provides a powerful method to study the Golgi in health and disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Jason E. Cournoyer, Sarah D. Altman, Yang-le Gao, Catherine L. Wallace, Dianwen Zhang, Guo-Hsuen Lo, Noah T. Haskin, Angad P. Mehta
Summary: The evolutionary origin of photosynthetic eukaryotes had a significant impact on the evolution of complex lifeforms and global ecology. The endosymbiotic theory suggests that photosynthetic eukaryotes evolved through endosymbiosis between non-photosynthetic host cells and photosynthetic cyanobacterial or algal endosymbionts. However, our understanding of this important evolutionary transformation is limited. In this study, the authors engineered artificial, genetically tractable photosynthetic endosymbiosis between cyanobacteria and yeast, which can be used for evolutionary studies and synthetic biology applications.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Lin-Lin Hu, Hong-Ge Li, Bi-Yun Liao, Yi Xu, Shao-Chen Sun, Jun-Li Wang
Summary: In this study, the effects of NP exposure on oocyte quality were investigated using a mouse in vivo model. The results showed that NP exposure caused changes in organelle distribution and function, leading to decreased oocyte development ability.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biology
Eric D. Salomaki, Kristina X. Terpis, Sonja Rueckert, Michael Kotyk, Zuzana Kotykova Varadinova, Ivan Cepicka, Christopher E. Lane, Martin Kolisko
Summary: Apicomplexan lineages exhibit diverse functional restructuring of mitochondrial metabolism, likely driven by adaptations to parasitism and anaerobiosis. Our findings suggest that apicomplexans have evolved convergent adaptations, sharing characteristics such as morphology, energy metabolism, and intracellular lifestyle.
Article
Biochemical Research Methods
Akila Wijerathna-Yapa, Elke Stroeher, Ricarda Fenske, Lei Li, Owen Duncan, A. Harvey Millar
Summary: Autophagy is a catabolic process that plays a crucial role in degrading cytoplasmic proteins and organelles in a lysosome or vacuole-dependent manner. Proteomic studies have helped uncover the protein regulatory mechanisms of autophagy, including the identification of receptor and cargo proteins and protein-protein interactions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Alexander G. Bury, Angela Pyle, Fabio Marcuccio, Doug M. Turnbull, Amy E. Vincent, Gavin Hudson, Paolo Actis
Summary: Intracellular heterogeneity is important for cellular physiology and diseases. This study developed a subcellular biopsy technology to isolate organelles, such as mitochondria, from human tissue. The technology was shown to be able to isolate organelles beyond the operational limit of laser capture microdissection (LCM) in human tissue.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Si Si Liew, Xiaofei Qin, Jia Zhou, Lin Li, Wei Huang, Shao Q. Yao
Summary: This review summarizes recent developments and strategies in the smart design of nanocarriers for mitochondria-targeted therapeutics, including subcellular targeting, stimuli-responsive nanocarriers, and synergistic therapy, while also discussing existing challenges and unexplored therapeutic opportunities.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Materials Science, Biomaterials
Naoto Yoshinaga, Keiji Numata
Summary: Mitochondria are crucial for cellular metabolism and energy production, and mutations in mitochondrial DNA can lead to inherited diseases. Strategies targeting mitochondria for gene therapy have been developed, but further research and optimization are needed.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Pharmacology & Pharmacy
Han Wang, Guiqian Fang, Huimin Chen, Maomao Hu, Yajuan Cui, Boyang Wang, Yudong Su, Yu Liu, Bo Dong, Xintian Shao
Summary: The study developed a new lysosome biosensor CNN for tracking lysosomes in living cells under both physiological and pathological conditions. High glucose conditions induced stress in lysosomes and altered the morphology of mitochondria.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Varsha Mathur, Eric D. Salomaki, Kevin C. Wakeman, Ina Na, Waldan K. Kwong, Martin Kolisko, Patrick J. Keeling
Summary: In this study, the evolution of cryptic plastids in apicomplexans, chrompodellids, and squirmids was investigated. The loss of plastid genome was found to be common, and lineage- and species-specific plastid proteins were unexpectedly discovered, suggesting the presence of evolutionary innovations and new functional and metabolic capabilities.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Review
Cell Biology
T. Bradley Willingham, Peter T. Ajayi, Brian Glancy
Summary: Mitochondria exhibit heterogeneity in form and function across different cell types and even within single cells. Distinct subpopulations of mitochondria have been identified in skeletal muscle cells, but the mechanisms by which this specialization contributes to energy homeostasis in working muscles remain unclear. Advances in cellular imaging technologies have opened up new potential avenues for further investigation into mitochondrial heterogeneity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yufei Wang, Yi-Feng Wang, Xianlei Li, Yuqing Wang, Qianqian Huang, Xiaowei Ma, Xing-Jie Liang
Summary: This study demonstrates the controllable regulation of mitochondria by gold nanoparticles, highlighting the importance of understanding the intrinsic capability of nanoparticles in biological processes. The nanoparticles induce size-dependent mitochondrial fission without causing detectable cell injury, providing practical guidance for nanolevel regulation.
Article
Chemistry, Multidisciplinary
Rashmi Yadav, Subrata Munan, Mudassar Ali, Koyeli Mapa, Animesh Samanta
Summary: A PET-based fluorescent probe, PM-C2, was developed for monitoring pH dynamics during mitochondrial dysfunctions. Compared to the other derivatives, PM-C2 showed higher fluorescence intensity and stronger pH sensitivity, making it suitable for tracking mitochondrial damage-induced pH alterations. The probe demonstrated biocompatibility, photostability, and mitochondria-specific targeting. The research was rated 8 out of 10.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Review
Cell Biology
Paul Dowling, Dieter Swandulla, Kay Ohlendieck
Summary: This review discusses the main bioanalytical methods used in the proteomic characterization of skeletal muscle tissues and the impact of skeletal muscle proteomics on whole-body physiology. Mass spectrometric studies have greatly improved our understanding of protein diversity and muscle tissue, which can be integrated with other omics-type methods to establish a systems biology view of skeletal muscle function.
Article
Microbiology
Fumika Mi-ichi, Akira Nozawa, Hiroki Yoshida, Yuzuru Tozawa, Tomoyoshi Nozaki
Article
Parasitology
Josephine E. Siregar, Genji Kurisu, Tamaki Kobayashi, Motomichi Matsuzaki, Kimitoshi Sakamoto, Fumika Mi-ichi, Yoh-ichi Watanabe, Makoto Hirai, Hiroyuki Matsuoka, Din Syafruddin, Sangkot Marzuki, Kiyoshi Kita
PARASITOLOGY INTERNATIONAL
(2015)
Article
Multidisciplinary Sciences
Fumika Mi-ichi, Tomofumi Miyamoto, Shouko Takao, Ghulam Jeelani, Tetsuo Hashimoto, Hiromitsu Hara, Tomoyoshi Nozaki, Hiroki Yoshida
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biochemistry & Molecular Biology
Herbert J. Santos, Kenichiro Imai, Yuki Hanadate, Yoshinori Fukasawa, Toshiyuki Oda, Fumika Mi-ichi, Tomoyoshi Nozaki
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2016)
Article
Parasitology
Sharmina Deloer, Risa Nakamura, Fumika Mi-ichi, Keishi Adachi, Seiki Kobayashi, Shinjiro Hamano
PARASITOLOGY INTERNATIONAL
(2016)
Review
Microbiology
Fumika Mi-ichi, Hiroki Yoshida, Shinjiro Hamano
Article
Biochemistry & Molecular Biology
Fumika Mi-ichi, Tomofumi Miyamoto, Hiroki Yoshida
MOLECULAR MICROBIOLOGY
(2017)
Article
Multidisciplinary Sciences
Honglian Tong, Yasunobu Miyake, Fumika Mi-ichi, Yoichiro Iwakura, Hiromitsu Hara, Hiroki Yoshida
Article
Multidisciplinary Sciences
Shinsuke Yasukawa, Yoshiyuki Miyazaki, Chika Yoshii, Mako Nakaya, Naoko Ozaki, Shuji Toda, Etsushi Kuroda, Ken-ichi Ishibashi, Tomoharu Yasuda, Yohei Natsuaki, Fumika Mi-ichi, Ei'ichi Iizasa, Takeshi Nakahara, Masanori Yamazaki, Kenji Kabashima, Yoichiro Iwakura, Toshiyuki Takai, Takashi Saito, Tomohiro Kurosaki, Bernard Malissen, Naohito Ohno, Masutaka Furue, Hiroki Yoshida, Hiromitsu Hara
NATURE COMMUNICATIONS
(2014)
Article
Immunology
Fumika Mi-ichi, Yasunobu Miyake, Vo Kha Tam, Hiroki Yoshida
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Fumika Mi-ichi, Hiroki Yoshida
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Infectious Diseases
Fumika Mi-ichi, Takeshi Ishikawa, Vo Kha Tam, Sharmina Deloer, Shinjiro Hamano, Tsuyoshi Hamada, Hiroki Yoshida
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Chemistry, Medicinal
Noriko Shinjyo, Hideyuki Nakayama, Kanji Ishimaru, Kenji Hikosaka, Fumika Mi-ichi, Kazumi Norose, Hiroki Yoshida
JOURNAL OF NATURAL MEDICINES
(2020)
Article
Parasitology
Eman Abdelazeem Abuelwafa Mousa, Miako Sakaguchi, Risa Nakamura, Osama Hussein Abdella, Hiroki Yoshida, Shinjiro Hamano, Fumika Mi-ichi
Article
Microbiology
Fumika Mi-ichi, Kazutaka Ikeda, Hiroshi Tsugawa, Sharmina Deloer, Hiroki Yoshida, Makoto Arita
Summary: This study identified dihydroceramides (Cer-NDSs) containing very long N-acyl chains (C-26 to C-30) as a key metabolite for Entamoeba encystation through state-of-the-art untargeted lipidomics. These Cer-NDSs are critical for generating membrane impermeability, enabling the parasite to exhibit dormancy as a cyst that resists substances and prevents water loss. Ceramide metabolism is essential for Entamoeba to maintain its parasitic lifestyle.