Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 39, Issue 1, Pages 11-19Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826060802589460
Keywords
2,4-D; Herbicides; Microbial biosensors; Pseudomonas putida; Screen printed electrodes
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Amperometric biosensors using Pseudomonas putida cells as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). After the adaptation process of Pseudomonas putida to 2,4-D, cells were immobilized onto the screen printed graphite electrodes (SPG) as well as Clark oxygen probe by gelatin and glutaraldehyde. Optimum pH, temperature, and stability of the biosensor were investigated. Substrate specificities for various phenolic compounds were also searched. In repeatability studies, variation coefficients and standard deviations for both type of systems were calculated; SPG and Clark electrodes were calculated and results are given as a comparison of two systems. Finally, the biosensors were applied to 2,4-D determination in a real herbicide sample.
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