Journal
PRENATAL DIAGNOSIS
Volume 32, Issue 10, Pages 933-942Publisher
WILEY-BLACKWELL
DOI: 10.1002/pd.3936
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Funding
- IWT [SBO 60848]
- FWO [G.0320.07]
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Objective Our knowledge about miscarriages mainly concerns pregnancies of at least 8?weeks gestation. Information about the morphology and the genetic determinants of early aborted embryos remains limited. In addition, it is known that aneuploidies account for less than half of recurrent spontaneous abortions. We hypothesized that (recurrent) early pregnancy losses might have other genetic causes. Method Products of conception from 51 couples with at least one previous miscarriage were collected by hystero-embryoscopy. The extracted DNA was analyzed by low resolution array comparative genomic hybridization and high resolution single nucleotide polymorphism arrays to detect aneuploidies, polyploidies, submicroscopic copy number variants or copy neutral loss of heterozygosity. Results Chromosomal aberrations were identified in 65.6% (21/32) of miscarriages and in 89% (8/9) of anembryonic cases. Interestingly, 4/11 chromosomally euploid embryos contained regions of loss of heterozygosity >5?Mb, suggesting the miscarriages might be due to an underlying lethal recessive disease. Conclusion Hystero-embryoscopic biopsy followed by array comparative genomic hybridization is a valuable diagnostic tool for early and recurrent miscarriages. Genome-wide high resolution single nucleotide polymorphism microarray analysis of a larger group of miscarriages could provide more insight into the genetic causes of recurrent spontaneous abortion. (c) 2012 John Wiley & Sons, Ltd.
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