Journal
POULTRY SCIENCE
Volume 91, Issue 10, Pages 2450-2453Publisher
POULTRY SCIENCE ASSOC INC
DOI: 10.3382/ps.2012-02375
Keywords
Riemerella anatipestifer; gyrB gene; polymerase chain reaction
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Funding
- Special Fund for Agro-scientific Research in the Public Interest [201003012]
- Agricultural Research System of China [CARS-43-8]
- National Science and Technology Support Program for Agriculture [2011BAD34B03]
- Changjiang Scholars and Innovative Research Team in University [PCSIRT0848]
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A pair of PCR primers was designed and synthesized to amplify a gyrB gene sequence from Riemerella anatipestifer (RA). A fragment of 194 bp was detected in RA-positive isolates, whereas other isolates were negative, which confirmed the high specificity of the primers and PCR conditions. As little as 1.6 x 10(4) cfu/mL of cultural liquid was required by this method. We compared a 16S rRNA sequence-based PCR method and a Biolog bacterial identification system used in the detection and identification of suspicious isolates of RA in clinical tests. The results showed that the gyrB-based PCR was consistent with the results of the Biolog identification system and was more specific. By applying the gyrB-PCR to detect RA strains in 56 duck livers, a positive rate of 46% (26/56) was observed, whereas the positive rate of 85 throat swabs from clinically healthy ducks was 11%. Thus, this method could be used for the epidemiological investigation and preliminary isolate identification of RA.
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