Journal
POULTRY SCIENCE
Volume 91, Issue 12, Pages 3202-3208Publisher
ELSEVIER
DOI: 10.3382/ps.2012-02552
Keywords
Riemerella anatipestifer; adhesion; invasion; pathogenesis; duck embryo fibroblast
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Funding
- Changjiang Scholars and Innovative Research Team in University [PCSIRT0848]
- China Agricultural Research System [CARS-43-8]
- Agro-scientific Research in the Public Interest [201003012]
- National Science and Technology Support Program for Agriculture [2011BAD34B03]
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Here, we investigated adhesion and invasion of Riemerella anatipestifer (RA) to primary duck embryo fibroblast (DEF) cells. The ability of RA to adhere to, and more importantly, to invade DEF cells was demonstrated by using a gentamicin invasion assay and was confirmed by transmission electron microscopy (TEM). Adhesion of RA could be found by TEM after 1 h of inoculation. Both apoptosis and necrocytosis of DEF were indicated by TEM after 10 h of incubation, which suggested a complex mechanism of DEF cell death induced by RA. Our results showed that internalized RA had the ability to leave the DEF cells. Inhibition studies indicated that RA proteins play a role in adhesion. Moreover, invasion of RA to DEF cells was shown to require rearrangement of actin microfilaments and microtubular cytoskeletal elements. Because the adhesion and invasion ability of RA to DEF cells could be demonstrated in vitro, similar processes might occur in vivo, where DEF cells play a crucial role in the diffusion of RA in ducks.
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