Article
Biotechnology & Applied Microbiology
Luciano dos Santos Bersot, Neila Rita Carbonera, Carolina Dias Rodrigues Valcanaia, Cibeli Viana, Luis Augusto Nero
Summary: This study revealed the high prevalence of Salmonella Heidelberg in poultry processing facilities and the widespread multidrug resistance of S. enterica. Cross-contamination and persistence of Salmonella Heidelberg were observed, along with high resistance to cephalosporins and extended-spectrum b-lactamases production in some isolates. These findings highlight the potential public health consequences of multidrug-resistant Salmonella contamination in the poultry chain.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Food Science & Technology
Xianying Zeng, Suling Lv, Cong Qu, Lan Lan, Dongmei Tan, Xiugui Li, Li Bai
Summary: The study investigated the serotype, antimicrobial resistance, and molecular typing of 843 Salmonella isolates recovered from diarrheic patients in Guangxi, China from 2014 to 2017. The results highlighted the diversity of Salmonella serotypes and the high prevalence of emerging multidrug-resistant (MDR) Salmonella in Guangxi, emphasizing the need for enhanced surveillance of these MDR isolates to protect public health.
Article
Public, Environmental & Occupational Health
Shaojun Wang, Xinmeng Liao, Zhiying Xiong, Qijie Lin, Junping Wen, Chenggang Xu, Xiaoyun Qu, Kaifeng Chen, Jianmin Zhang
Summary: The study found that the predominant Salmonella enterica serotype Kentucky ST314 in China carries multidrug resistance, primarily mediated by plasmid-borne quinolone resistance genes. These strains can transmit across different regions and hosts, promoting the spread of ciprofloxacin resistance.
ZOONOSES AND PUBLIC HEALTH
(2021)
Article
Microbiology
Yasser M. Sanad, Nesreen H. Aljahdali, Bijay K. Khajanchi, Rajesh Nayak, Ashraf Khan, Steven L. Foley
Summary: Salmonella causes a large number of infections and deaths in the U.S. Javiana serotype strains from Arkansas show high antimicrobial resistance. Genetic analysis reveals the diversity and distribution of the strains. Salmonella persist in human populations for multiple years.
Article
Biochemical Research Methods
Amber N. Rogers, Maya K. Mastronardo, Tsion G. Mekonnen, Ana Maria Soto
Summary: RNA research is an active field that often requires large amounts of purified RNA. One common purification method involves using electroelution to recover RNA from gel pieces. In this study, two electroeluters with different chamber designs were compared and both were found to effectively recover comparable amounts of purified RNA.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Christoph J. Burckhardt, John D. Minna, Gaudenz Danuser
Summary: Co-immunoprecipitation is widely used in studying protein-protein interactions, but requires considerable optimization, and results are often presented non-quantitatively. This protocol offers an optimized workflow using semi-quantitative immunoblot densitometry for increased reliability and reproducibility.
Article
Agriculture, Dairy & Animal Science
Larissa Di Marzo, Joice Pranata, David M. Barbano
Summary: The study compared the estimation of milk casein percentage using SDS-PAGE and Kjeldahl methods, finding that Kjeldahl underestimated the proteolytic damage to casein in milk.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Polymer Science
Hiroyuki Ohshima
Summary: This study develops a general theory for the time-dependent transient electrophoresis of a weakly charged spherical colloidal particle in a polymer gel medium. The Laplace transform of the transient electrophoretic mobility is derived by considering the hydrodynamic interaction between the particle and the gel medium based on the Brinkman-Debye-Bueche model. The theory covers both transient gel electrophoresis and transient free-solution electrophoresis, with the relaxation time for the former being shorter and decreasing as the Brinkman screening length decreases.
Article
Engineering, Environmental
Dongqi Wang, Yueyun Li, Helen A. Cope, Xiaoxiao Li, Peisheng He, Cong Liu, Guangyu Li, Sheikh M. Rahman, Nicholas B. Tooker, Charles B. Bott, Annalisa Onnis-Hayden, Jyoti Singh, Alistair Elfick, Ricardo Marques, Henning J. Jessen, Adrian Oehmen, April Z. Gu
Summary: This study demonstrated the use of single-cell Raman spectroscopy (SCRS) for characterizing intracellular polyPs in PAOs within complex environmental samples like EBPR systems. SCRS revealed distinct distribution patterns of polyP length in different PAO types and configurations of EBPR systems, as well as distinctive polyP features among PAO phenotypic sub-groups, indicating its potential for microdiversity-level analysis within EBPR communities.
Article
Multidisciplinary Sciences
Renato Lima Senra, Camilo Jose Ramirez-Lopez, Marcos Jorge Magalhaes-Junior, Joao Gabriel da Silva Neves, Edvaldo Barros, Bruna Waddington, Simone Eliza Facioni Guimaraes, Jose Domingos Guimaraes, Maria Cristina Baracat-Pereira
Summary: This study found that kallikrein proteoforms are involved in the seasonality of horse reproduction. During the breeding season, increased abundance and different types of kallikreins were observed, along with lower sperm motility, increased semen volume, and higher concentrations of glucose and cholesterol.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Ying Guan, Yanwan Li, Jin Li, Zhishuang Yang, Dekang Zhu, Renyong Jia, Mafeng Liu, Mingshu Wang, Shun Chen, Qiao Yang, Ying Wu, Shaqiu Zhang, Qun Gao, Xumin Ou, Sai Mao, Juan Huang, Di Sun, Bin Tian, Anchun Cheng, Xinxin Zhao
Summary: In this study, Salmonella isolates from waterfowl samples in Sichuan, China were systematically examined for serotypes, antimicrobial resistance profiles, and genotypic characteristics. The results showed diverse serotypes and high rates of multidrug resistance among the isolates. The study highlights the potential risks to public health posed by Salmonella prevalent in waterfowl farms in Sichuan.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Mate Sagi-Kazar, Eva Sarvari, Barnabas Cseh, Levente Illes, Zoltan May, Csaba Hegedus, Attila Barocsi, Sandor Lenk, Katalin Solymosi, Adam Solti
Summary: The uptake of iron by chloroplasts does not solely rely on photosynthetic functions, as demonstrated by the ability of etioplasts to acquire iron even in the absence of photosynthetic activity. This likely occurs through the internal reducing capacity of etioplasts supporting the reduction-based iron uptake.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
N. Al-Gallas, N. Khadraoui, H. Hotzel, H. Tomaso, H. El-Adawy, H. Neubauer, K. Belghouthi, K. Ghedira, H. K. Gautam, B. Kumar, D. Laouini, S. Zarrouk, M. S. Abbassi, R. B. Aissa
Summary: The study revealed the presence of quinolone multidrug-resistant Salmonella in humans and animals in Tunisia, with the first report of S. Typhimurium ST34 in Africa and qnrB19 in Tunisia. The research not only described the epidemiological situation of quinolone resistance in S. Kentucky and Typhimurium in Tunisia, but also analyzed the genetic resistance determinants and molecular mechanisms associated with phenotypic antibiotic resistance.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Immunology
Seema Irfan, Mohammad Zeeshan, Salima Rattani, Joveria Farooqi, Sadia Shakoor, Rumina Hasan, Afia Zafar
Summary: From 2013 to 2018, although there was an increase in localized extensively drug-resistant Salmonella Typhi strains in organ infections in Pakistan, there was no increase in the proportion of such isolates compared to non-extensively drug-resistant isolates.
EMERGING INFECTIOUS DISEASES
(2021)
Review
Chemistry, Analytical
Laszlo Hajba, Sunkyung Jeong, Doo Soo Chung, Andras Guttman
Summary: This review provides an overview of the fundamental principles, methodologies, strengths, and weaknesses of capillary gel electrophoresis of proteins. It covers the historical background, new developments, and applications in biopharmaceutical, biomedical, food, and agriculture fields. Different subsets of the method and relevant protein labeling techniques are critically discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Jesper Tessin, Judith Rohde, Arne Jung, Nicole Kemper, Jochen Schulz
Summary: In this study, samples from poultry house environment and culled birds were collected, and E. cecorum was successfully isolated. The principle of isolating the pathogen by streaking on a chromogenic agar may motivate researchers to investigate transmission routes and optimize biosecurity measures.
Article
Agriculture, Dairy & Animal Science
Junqin Zhang, Zimin Xie, Yanlin Pan, Zuoxin Chen, Yunzhen Huang, Linlin Li, Jiawen Dong, Yong Xiang, Qi Zhai, Minhua Sun, Xingying Li, Shujian Huang, Ming Liao
Summary: In recent years, there has been an increase in the occurrence of fowl adenovirus 2 (FAdV-2) in China, posing a significant threat to the poultry industry. This study investigated the epidemiology, phylogenetic relationship, genomic characteristics, and pathogenicity of FAdV-2. The results showed that FAdV-2 was the most commonly detected serotype among various FAdV serotypes. Genetic analysis revealed that these FAdV-2 strains formed a distinct branch within the FAdV-D group and had a high nucleotide similarity with reference strains. Recombination analysis identified recombination events between FAdV-2 and FAdV-11 in some strains. Pathogenicity experiments demonstrated that a recombinant FAdV-2 strain caused a high mortality rate and more severe disease in chickens. These findings contribute to our understanding of FAdV-2 and provide a basis for vaccine development.
Article
Agriculture, Dairy & Animal Science
Ebtsam Iraqi, Ali Abdel Hady, Nadia Elsayed, Hanaa Khalil, Amina El-Saadany, Karim El-Sabrout
Summary: Thermal manipulation during egg incubation, particularly at 12 to 18 days, has positive effects on embryonic development, hatching characteristics, chick quality, as well as posthatch thermotolerance and performance.
Article
Agriculture, Dairy & Animal Science
Yong Liu, Mingxin Song, He Bai, Chunhua Wang, Fei Wang, Qi Yuan
Summary: Dietary curcumin supplementation can significantly improve egg quality of quails in the late laying period, primarily by improving lipid metabolism and selectively regulating the intestinal microbial community.
Article
Agriculture, Dairy & Animal Science
Lei Zhang, Zhixuan Song, Peiyao Li, Xingju Song, Xinming Tang, Dandan Hu
Summary: This study evaluated the vaccine potential of recombinant proteins for avian coccidiosis caused by Eimeria. The results showed that the rEten5-B protein can trigger immune protection against E. tenella and may be a potential and effective subunit vaccine for the control of coccidiosis in poultry.
Article
Agriculture, Dairy & Animal Science
Milan Kumar Sharma, Amit Kumar Singh, Doyun Goo, Venkata Sesha Reddy Choppa, Hanseo Ko, Hanyi Shi, Woo Kyun Kim
Summary: The study investigates the effect of Eimeria infection on gastrointestinal physiology, growth performance, and egg production of Hy-Line W-36 laying hens during peak production. Results show that Eimeria infection negatively affects body weight, feed intake, immune response, and nutrient transporter gene expression. Egg production temporarily ceases in hens infected with high and medium-high dosages of Eimeria.
Article
Agriculture, Dairy & Animal Science
Elwy A. Ashour, Mahmoud Kamal, Hayman A. A. Altaie, Ayman A. Swelum, Gamaleldin M. Suliman, Guillermo Tellez-Isaias, Mohamed E. Abd El-Hack
Summary: This study evaluated the effects of CP and ME on the performance and nutritional value of Japanese quail lay chicken. The results showed that protein and energy levels significantly influenced all productive outcomes except feed conversion ratio. The combination of dietary energy and protein levels had a substantial effect on all egg quality trials evaluated. It is recommended to feed layer Japanese quail between the ages of 8 and 20 weeks during the summer with a dietary energy content of 2,900 kcal ME/kg and 20% CP.
Article
Agriculture, Dairy & Animal Science
J. E. Doornweerd, R. F. Veerkamp, B. de Klerk, M. van der Sluis, A. C. Bouwman, E. D. Ellen, G. Kootstra
Summary: Tracking group-housed individual broilers using video can provide valuable information on their health, welfare, and performance. This study conducted a comprehensive analysis on broiler tracking on video and potential tracking errors, highlighting the potential and challenges of phenotyping broiler locomotion. The results emphasized the importance of addressing tracking errors, potential algorithm improvements, and the need for an external animal identification system for efficient locomotion phenotyping.
Article
Agriculture, Dairy & Animal Science
Jiayu Wu, Yanan Wang, Yu An, Changyu Tian, Lingfeng Wang, Zuhong Liu, Desheng Qi
Summary: This study compared the gene expression differences in liver tissues of laying hens at different ages using RNA sequencing, and found that the transport and metabolism of amino acids changed during the growing period, which influenced the growth and development of the hens.
Article
Agriculture, Dairy & Animal Science
J. Raabe, G. Raveendran, W. Otten, K. Homeyer, T. Bartels
Summary: This study investigated the effects of different flicker frequencies on the behavior, performance, and stress response in male turkeys. The results showed that flicker frequencies of 165 Hz or higher had no detrimental effect on growth performance, injurious pecking, or endocrine stress response in the turkeys.
Article
Agriculture, Dairy & Animal Science
Adamu Mani Isa, Yanyan Sun, Yuanmei Wang, Yunlei Li, Jingwei Yuan, Aixin Ni, Hui Ma, Lei Shi, Hailai Hagos Tesfay, Yunhe Zong, Panlin Wang, Pingzhuang Ge, Jilan Chen
Summary: This study sequenced mRNAs and lncRNAs in the ovary stroma of specific chicken breeds and their reciprocal crossbreds to understand the molecular mechanism of heterosis for egg production. Results showed that genes with nonadditive expression were related to follicle growth and atresia as well as oxidative stress in the crossbred hens.
Article
Agriculture, Dairy & Animal Science
Zhepeng Wang, Qiu Chen, Yiwei Wang, Yulu Wang, Ruifang Liu
Summary: The study investigates the functional variants associated with chicken eggshell color in the SLCO1B3 gene by measuring the eggshell color of Lueyang black-boned chickens and analyzing the genetic data. It identifies 15 variants in SLCO1B3 that are significantly associated with eggshell color. However, the functional analysis shows that one specific variant, 1B3_SNP108, does not have a significant effect on promoter activity. This study highlights the importance of SLCO1B3 and its variants in breeding eggshell color.
Article
Agriculture, Dairy & Animal Science
P. Yin, Q. Tong, B. M. Li, W. C. Zheng, Y. Wang, H. Q. Peng, X. L. Xue, S. Q. Wei
Summary: This study investigated the welfare and health of laying hens in a multitier system. The results showed that the spatial distribution of hens changed with increasing age, and lower-ranking hens preferred to stay in lower tiers to avoid competition but still had access to resources. This improved the welfare and health of the hens near the end of the laying cycle.
Article
Agriculture, Dairy & Animal Science
Wenwen Liang, Siqiong Jie, Yiqin Wang, Xiaowei Wen, Yanling Xiong, Xiaoxi Lin, Zhengrong Lv
Summary: This study examined the effect of risk perceptions on preventive behavior among vendors in the live poultry wholesale market and the live poultry retail market in Guangdong Province using the health belief model and structural equation modeling. The results showed that perceived severity and perceived benefits positively influenced vendors' self-efficacy in the wholesale market, while perceived barriers negatively affected self-efficacy in both the wholesale and retail markets. Moreover, cues to action mediated the relationship between perceived severity, perceived benefits, and self-efficacy, and also mediated the effect of perceived susceptibility among wholesale market vendors.
Article
Agriculture, Dairy & Animal Science
Shreeya Sharma, Raveendra R. Kulkarni, Shayan Sharif, Hosni Hassan, Mohammadali Alizadeh, Scott Pratt, Khaled Abdelaziz
Summary: The study investigates the impact of exposing chick embryos to antigens via in ovo delivery of poultry-specific lactobacilli on the expression of genes associated with early bursal development and maturation. Results indicate that the supplementation of poultry-specific lactobacilli to chick embryos can accelerate the development and immunological maturation of the bursa of Fabricius.