Article
Microbiology
Sean N. Avedissian, Gwendolyn Pais, Jiajun Liu, J. Nicholas O'Donnell, Thomas P. Lodise, Michael Neely, Walter C. Prozialeck, Peter C. Lamar, Leighton Becher, Marc H. Scheetz
Summary: The study identified that in a rat model, both the dosage and frequency of vancomycin administration significantly influenced the risk of kidney injury, with exposure-response relationships observed between pharmacokinetic exposure and kidney injury molecule-1 levels.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Chemistry, Analytical
Leonidas Mavroudakis, Kyle D. Duncan, Ingela Lanekoff
Summary: A new method incorporating crown ether molecules in the solvent for nano-DESI MSI allows for quantitative imaging of alkali metal ions and endogenous lipids/metabolites. Applied to an ischemic stroke model, this method revealed dynamic tissue sodium and potassium concentrations, along with changes in lipids, neurotransmitters, and amino acids.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Kasper K. Krestensen, Ron M. A. Heeren, Benjamin Balluff
Summary: Mass spectrometry imaging (MSI) has evolved from a niche technique to a widely used spatial biology tool. This review provides an overview of state-of-the-art MSI applications in both established and emerging biomedical fields, serving as a reference framework for new researchers in the field.
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Daojiang Yu, Peng Lai, Tao Yan, Kai Fang, Lei Chen, Shuyu Zhang
Summary: MMP2 plays a crucial role in biological processes, but its spatial analysis is challenging due to its large molecular weight and low content. Researchers have developed a specific MMP2 probe to accurately measure its expression in tissues, which may help study the impact of proteases on malignancy.
FRONTIERS IN CHEMISTRY
(2021)
Article
Plant Sciences
Wenxiang Fan, Yuangui Yang, Linnan Li, Linhong Fan, Zhengtao Wang, Li Yang
Summary: This study comprehensively revealed the chemical diversity and dynamic transformation pattern of Panax notoginseng during processing, providing insights into the distinct bioactivities between raw and processed Panax notoginseng. The proposed approach is valuable for quality evaluation and control of natural product processing.
Article
Multidisciplinary Sciences
G. Guo, M. Papanicolaou, N. J. Demarais, Z. Wang, K. L. Schey, P. Timpson, T. R. Cox, A. C. Grey
Summary: Spatial proteomics using MALDI-MSI is a powerful tool for direct detection of protein distribution in tissues but faces challenges in peptide quantification and identification. The development of HIT-MAP provides an open-source bioinformatics workflow for accurate protein annotation and spatial mapping from MALDI-MSI data.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Bingbing Liu, Xuanlin Meng, Kun Li, Jinggong Guo, Zongwei Cai
Summary: This study used MALDI-MSI to investigate the spatial distribution of lipids in cottonseeds, revealing heterogeneous distribution patterns of different lipid species within the seeds. The roles of these lipids in metabolic pathways were analyzed, and the relationship between their spatial distribution and functions was studied. The unique distribution patterns of lipid metabolites revealed by MSI may offer new insights into the spatial compartmentation of lipid metabolism in plants and can potentially aid genetic engineering efforts when combined with transcriptomics and proteomics.
Article
Chemistry, Analytical
Bo-Jhang Lin, Tien-Chueh Kuo, Hsin-Hsiang Chung, Ying-Chen Huang, Ming-Yang Wang, Cheng-Chih Hsu, Po-Yang Yao, Yufeng Jane Tseng
Summary: Mass spectrometry imaging (MSI) is a powerful tool for investigating the spatial distribution of molecules in samples. However, analyzing complex biological systems often requires multiple analytical techniques. Image registration is a critical step for combining MSI with other imaging techniques, but it can be challenging due to differences in spatial resolution and image characteristics. In this study, a web service called MSI Registrar (MSIr) was developed for automatic registration between MSI and histology, providing a more objective and efficient way to analyze molecular components in tissues.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
M. Sejalon-Cipolla, P. Bruyat, S. Bregant, C. Malgorn, L. Devel, G. Subra, S. Cantel
Summary: MALDI-MS is crucial for detecting and identifying molecules involved in biological pathways, but many biomarkers remain undetectable due to various limitations. To overcome these challenges, labeling strategies and activity-based probes have been developed. Matrix-free derivatization techniques are currently gaining attention.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Qiuqin Zhou, Annabelle Fueloep, Carsten Hopf
Summary: MALDI-MSI is a rapidly growing technique for visualizing the distribution of biomolecules in tissue sections, with challenges such as sensitivity and specificity. Recent developments in small organic MALDI matrices and on-tissue chemical derivatization reagents play a crucial role in improving image quality and expanding application areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Nannan Li, Shuqin Li, Qirou Wang, Shuyu Yang, Yinuo Hou, Yan Gao, Xiangyang Zhang, Min Zhang, Haixia Chen
Summary: This study applied MALDI-TOF MSI and QTOF LC-MS/MS techniques to investigate the spatial distribution changes of low molecular weight compounds in garlic tissue during Laba garlic processing. It was found that some bioactive compounds were transformed or leached out during the processing, while new compounds related to pigments emerged. This study provides a basis for understanding the spatial distributions and changes of compounds in garlic tissue during Laba garlic processing, which may affect the bioactivities of garlic.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biophysics
Xian-Na Wang, Yahui Song, Weiwei Tang, Ping Li, Bin Li
Summary: Microfluidic technology provides an ideal platform for in situ screening of enzyme inhibitors and activators from natural products. This study developed a surface-modified ITO glass-PDMS hybrid microfluidic chip, which integrated fluorescence imaging and MALDI MSI for evaluating thrombin interaction with potential inhibitors. The results showed that luteolin, cynaroside, and baicalin have good thrombin inhibitory activity, making them promising candidates for further research in developing inhibitors for thrombin-related diseases.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Pharmacology & Pharmacy
Qun Wang, Ya Shi, Zengguang Wu, Xinli Song, Jinfang Luo, Hong Yang, Xiaolan Chen, Xingde Liu
Summary: The study aimed to investigate the effects of HOD on diabetic nephropathy and the tissue distribution of its active ingredients in normal and DN mouse models. Treatment with HOD in DN mice led to improved renal function and decreased key markers of DN. The tissue distribution of HOD active ingredients differed in mice with DN compared to normal mice, indicating a potential impact of the pathological state of diabetic nephropathy.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Kaitlin C. Lesco, Steven M. Rowland, S. Kim Ratanathanawongs Williams, Lieve M. L. Laurens
Summary: This study used matrix-assisted laser desorption ionization imaging mass spectrometry to investigate the localization of lipids in immobilized Spirulina filaments. Optimization of matrix selection and deposition methods improved signal abundance and spatial resolution, and allowed for identification of distinct lipid composition regions. This work provides a methodology for micro-ecological untargeted molecular phenotyping.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Biochemical Research Methods
Ekta Patel, Malcolm R. Clench, Andy West, Peter S. Marshall, Nathan Marshall, Simona Francese
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2015)
Editorial Material
Biochemical Research Methods
Laura M. Cole, Malcolm R. Clench
PROTEOMICS CLINICAL APPLICATIONS
(2015)
Article
Chemistry, Analytical
Cristina Russo, Neil Brickelbank, Catherine Duckett, Steve Mellor, Stephen Rumbelow, Malcolm R. Clench
ANALYTICAL CHEMISTRY
(2018)
Article
Chemistry, Applied
E. E. L. Lewis, M. R. T. Barrett, L. Freeman-Parry, R. A. Bojar, M. R. Clench
INTERNATIONAL JOURNAL OF COSMETIC SCIENCE
(2018)
Article
Biochemical Research Methods
Bryn Flinders, Josie Morrell, Peter S. Marshall, Lisa E. Ranshaw, Malcolm R. Clench
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Karol Jelonek, Monika Pietrowska, Malgorzata Ros, Adam Zagdanski, Agnieszka Suchwalko, Joanna Polanska, Michal Marczyk, Tomasz Rutkowski, Krzysztof Skladowski, Malcolm R. Clench, Piotr Widlak
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2014)
Article
Biochemical Research Methods
Laura M. Cole, Joanne E. Bluff, Vikki A. Carolan, Martyn N. Paley, Gillian M. Tozer, Malcolm R. Clench
Article
Medicine, Legal
R. Bradshaw, S. Bleay, M. R. Clench, S. Francese
Article
Multidisciplinary Sciences
Eylan Yutuc, Roberto Angelini, Mark Baumert, Natalia Mast, Irina Pikuleva, Jillian Newton, Malcolm R. Clench, David O. F. Skibinski, Owain W. Howell, Yuqin Wang, William J. Griffiths
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Chemistry, Analytical
Lucy E. Flint, Gregory Hamm, Joseph D. Ready, Stephanie Ling, Catherine J. Duckett, Neil A. Cross, Laura M. Cole, David P. Smith, Richard J. A. Goodwin, Malcolm R. Clench
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Oana M. Voloaca, Calum J. Greenhalgh, Laura M. Cole, Malcolm R. Clench, Amy J. Managh, Sarah L. Haywood-Small
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2020)
Article
Biochemical Research Methods
Bryn Flinders, Josie Morrell, Peter S. Marshall, Lisa E. Ranshaw, Ron M. A. Heeren, Malcolm R. Clench
Summary: A straightforward method to generate 3D MALDI-MS images of selected molecules in lung tissue was presented, providing a deeper understanding of the distribution of molecules related to different anatomical features throughout the entire organ.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Oana M. Voloaca, Malcolm R. Clench, Gunda Koellensperger, Laura M. Cole, Sarah L. Haywood-Small, Sarah Theiner
Summary: This study reports the use of laser ablation-inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS) to analyze human malignant pleural mesothelioma (MPM) samples at the cellular level for the first time. The technique allows for simultaneous detection of asbestos fibers, metallome monitoring, and biomarker identification with high spatial resolution. The results provide insights into the pathology and potential toxic elements associated with MPM.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Marie-Claude Djidja, Emmanuelle Claude, Peter Scriven, David W. Allen, Vikki A. Carolan, Malcolm R. Clench
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2017)
