Article
Multidisciplinary Sciences
Stephanie Schumacher, Dirk Dedden, Roberto Vazquez Nunez, Kyoko Matoba, Junichi Takagi, Christian Biertuempfel, Naoko Mizuno
Summary: The interaction mechanism between Integrin alpha(5)beta(1) and fibronectin was studied, revealing significant conformational changes upon complex formation. Additionally, the resting state of the integrin adopts an incompletely bent conformation, challenging the existing model.
Article
Hematology
Emily Janus-Bell, Alexandra Yakusheva, Cyril Scandola, Nicolas Receveur, Usman Muhammad Ahmed, Clarisse Mouriaux, Catherine Bourdon, Cecile Loubiere, Anita Eckly, Yotis A. Senis, Mikhail A. Panteleev, Christian Gachet, Pierre H. Mangin
Summary: The study demonstrates that platelet integrin alpha 5 beta 1 plays a key role in fibrillar cellular fibronectin, but is dispensable in hemostasis and arterial thrombosis.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Chemistry, Multidisciplinary
Seungkuk Ahn, Upnishad Sharma, Krishna Chaitanya Kasuba, Nico Strohmeyer, Daniel J. J. Muller
Summary: This study introduces a simple and scalable method to engineer biomimetic 3D fibrillar fibronectin matrices and characterizes how fibroblasts sense and adhere to these matrices. The findings demonstrate that fibroblasts accelerate adhesion initiation and strengthening to fibrillar fibronectin matrices compared to globular fibronectin. This immediate sensing and adhesion to fibrillar fibronectin also guide migration speed, persistency, and proliferation range in fibroblasts.
Article
Biochemistry & Molecular Biology
Balik Dzhambazov, Tsvetelina Batsalova, Patrick Merky, Franziska Lange, Rikard Holmdahl
Summary: This study investigates the impact of different types of fibroblasts on the pathogenesis of rheumatoid arthritis (RA). The results show that only NIH/3T3 fibroblasts can activate T-cell hybridomas specific for the RA-associated autoantigen COL2, while other types of fibroblasts cannot. This activation occurs through contact-dependent intercellular interactions and the T-cell receptor (TCR) complex.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Seong Hee Kang, Dong-Ho Bak, Seung Sik Lee, Hyoung-Woo Bai, Byung Yeoup Chung, Bo Sun Kang
Summary: The study demonstrated that centipedegrass extract (CGE) exhibited significant cytoprotective effects against H2O2-induced cell damage, reducing ROS levels and apoptotic protein expression. Additionally, CGE showed protective effects against intracellular ROS and radiation exposure, upregulating anti-apoptotic signals and downregulating pro-apoptotic signals. These results suggest that CGE may be an effective candidate for treating oxidative stress-related diseases and providing radioprotection.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Engineering, Multidisciplinary
Katarina McGarry, Eelya Sefat, Taylor C. Suh, Kiran M. Ali, Jessica M. Gluck
Summary: Polymer scaffolds are widely used in tissue engineering to improve the repair and regeneration of damaged tissue. Natural polymers have better cellular adhesion and proliferation, while synthetic polymers are highly tunable. An evaluation of the cellular response to collagen-based and PLA scaffolds showed that NIH3T3 cells preferred collagen-based scaffolds. Incorporating cellular binding mechanisms from natural polymers into biodegradable synthetics offers a promising direction for biomaterials to become biomimetic.
Article
Polymer Science
Diana Querido, Tania Vieira, Jose Luis Ferreira, Celia Henriques, Joao Paulo Borges, Jorge Carvalho Silva
Summary: Hybrid scaffolds obtained by combining two or more biopolymers are studied in the context of tissue regeneration. This study produced a new type of hybrid polycaprolactone (PCL)/chitosan (CS) electrospun mat by controlling the deposition of CS flakes between PCL fibers. The morphology of the resulting fibers confirmed the presence of CS flakes. In vitro cell culture results showed that the addition of CS reduced cell adhesion and proliferation.
Article
Biochemistry & Molecular Biology
Marketa Havrdova, Iztok Urbancic, Katerina Barton Tomankova, Lukas Malina, Janez Strancar, Athanasios B. Bourlinos
Summary: The study reveals that QCDs can penetrate the cell nucleus and exhibit concentration-dependent effects internally. QCDs have different impacts on NIH/3T3 and L929 cells, with the former experiencing changes in cell cycle profile and the latter leading to cellular death. Additionally, the presence of QCDs in the nucleus affects the intranuclear environment and causes fluorescence blue shifts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Iruthayapandi Selestin Raja, Seok Hyun Lee, Moon Sung Kang, Suong-Hyu Hyon, Aravindha Raja Selvaraj, Kandasamy Prabakar, Dong-Wook Han
Summary: Wettability has a greater influence on cellular behaviors on nanofibrous scaffolds compared to surface morphology.
MATERIALS & DESIGN
(2022)
Article
Chemistry, Physical
Maria I. Echeverria I. Molina, Chi-An Chen, Jeniree Martinez, Perry Tran, Kyriakos Komvopoulos
Summary: Fully functional skin regeneration is still difficult after decades of research. Scaffold engineering, a leading field in skin substitutes, can optimize the biomolecular exchange to promote the formation of high-quality tissue. This study designed and evaluated a new class of three-dimensional fibrous scaffolds that induce keratinocyte differentiation through calcium leaching, demonstrating their potential for tissue engineering.
Article
Materials Science, Biomaterials
Yunxi Gao, Anthony Callanan
Summary: The study found that scaffolds with small surface depressions exhibited higher cell viability and DNA content for HepG2 cells, promoted gene expression of albumin, and showed higher ultimate tensile strength, indicating that small depressions may be preferred by HepG2 cells and have the potential to tailor liver cell responses.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Allen Sam Titus, Harikrishnan Venugopal, Mereena George Ushakumary, Mingyi Wang, Randy T. Cowling, Edward G. Lakatta, Shivakumar Kailasam
Summary: The study revealed the crucial role of fibronectin in regulating cardiac fibroblast function, mediated by DDR2 and Integrin-beta 1 signaling pathways. This complex mechanism involves collagen type I and fibronectin interacting with their receptors, offering insights into potential therapeutic targets for myocardial injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Arsalan D. Badaraev, Dmitrii Sidelev, Anna Kozelskaya, Evgeny N. Bolbasov, Tuan-Hoang Tran, Alexey Nashchekin, Anna B. Malashicheva, Sven Rutkowski, Sergei Tverdokhlebov
Summary: In this study, polymer scaffolds were fabricated and surface-modified by sputtering titanium targets, resulting in increased hydrophilicity and cell adhesion.
Article
Biochemistry & Molecular Biology
Kyle Selcer, Barathi Balasubramonian, Dylan Miller, Jade Kerr, Mia DiFrancesco, Sanjana Ojha, Rachel Urbano
Summary: The study characterized STS activity in mouse fibroblasts, revealing its distribution in cells and regulation by hormones like cortisol. Further research could shed light on the function of STS in fibroblasts and its relationship with local estrogen levels.
Article
Multidisciplinary Sciences
Wichurat Sakulpaptong, Isabelle A. Clairmonte, Britani N. Blackstone, Binnaz Leblebicioglu, Heather M. Powell
Summary: The goal of this study was to develop a low-cost, high-fidelity keratinized human gingival model for studying the implant-tissue interface. Electrospun collagen scaffolds were found to provide a scalable and cost-effective lamina propria and 3D engineered gingival that mimics the native tissue structure. The engineered tissue was used to investigate peri-implant soft tissue attachment to different dental materials.
Article
Construction & Building Technology
Jessica A. Rosewitz, Habibeh Ashouri Choshali, Nima Rahbar
CEMENT & CONCRETE COMPOSITES
(2019)
Article
Engineering, Biomedical
Sina Askarinejad, Joshua E. Johnson, Nima Rahbar, Karen L. Troy
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2019)
Article
Biophysics
Zachary E. Goldblatt, Habibeh Ashouri Choshali, Heather A. Cirka, Vivian Liang, Qi Wen, Dannel McCollum, Nima Rahbar, Kristen L. Billiar
BIOPHYSICAL JOURNAL
(2020)
Article
Construction & Building Technology
Shuai Wang, Rajib B. Mallick, Nima Rahbar
CONSTRUCTION AND BUILDING MATERIALS
(2020)
Article
Engineering, Biomedical
Emmanuel O. Onche, Salifu T. Azeko, John D. Obayemi, Oluwaseun K. Oyewole, Nneka B. Ekwe, Nima Rahbar, Winston O. Soboyejo
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2020)
Article
Engineering, Multidisciplinary
Sina Askarinejad, Faezeh Shalchy, Nima Rahbar
Summary: Nacre's exceptional mechanical properties are attributed to its multilayered structure, nanoscale features, and the role of interfaces. Understanding the interphase properties is crucial for enhancing material toughness.
COMPOSITES PART B-ENGINEERING
(2021)
Article
Nanoscience & Nanotechnology
T. Bond, A. Badmos, R. A. Ahmed, J. D. Obayemi, A. Salifu, N. Rahbar, W. O. Soboyejo
Summary: This paper presents a study on the indentation size effects in structural metals/alloys at the micron scale, including 6061 aluminum alloy, titanium, and Ti-6Al-4V. The size dependence of indentation hardness is explained using strain gradient plasticity theories. The study compares the resulting material length scales with microstructures and previously reported values, and discusses the implications of the results for plasticity and hardness modeling at the micron scale.
MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING
(2022)
Article
Biophysics
Habibeh Ashouri Choshali, Kristen L. Billiar, Nima Rahbar
Summary: In this study, the stress fields within cells were investigated using computational and experimental methods, revealing the presence of anisotropy. It was found that substantial stress concentration occurs in the central region, contradicting the assumption of uniform anisotropy. A more realistic non-uniform anisotropy model was introduced based on experimental observations, effectively eliminating stress concentration. The study provides a physics-based mechanism to explain the low alignment of stress fibers in cells and potentially explains certain biological phenomena.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Article
Engineering, Biomedical
Alycia Abbott, Mattea E. Gravina, Mobin Vandadi, Nima Rahbar, Jeannine M. Coburn
Summary: This study explored the impact of modifying lyophilization primary drying parameters on the properties of protein-based porous scaffolds. It was found that changing the drying duration and temperature can result in scaffolds with tunable properties. Additionally, the use of different concentrations of the protein solution led to different effects on in vitro cell culturing.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Materials Science, Biomaterials
Mobin Vandadi, Kenan P. Fears, Manoj K. Kolel-Veetil, Nima Rahbar
Summary: Biomaterials serve as an important inspiration for the development of strong and tough materials. A novel class of nanomaterials, synthesized through side-chain to-side-chain polymerization of cyclic beta-peptide rings, exhibits outstanding mechanical properties. Molecular dynamics simulations reveal that polymerized peptides can withstand stress and have high strain-to-failure values, compared to brittle behavior of unpolymerized peptides. Additionally, the strength of cyclic peptides is higher in water than in a vacuum.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Mobin Vandadi, Kenan P. Fears, Manoj K. Kolel-Veetil, Nima Rahbar
Summary: Biomaterials serve as an important inspiration for the development of strong and tough materials, leading to the recent development of improved synthetic materials. Through the side-chain-to-side-chain polymerization of cyclic beta-peptide rings, a new class of nanomaterials with exceptional mechanical properties has been introduced. Molecular dynamics simulations are used to explore the mechanics of this polymerization process and demonstrate the differences in strength between polymerized and unpolymerized cyclic peptides. The simulations are conducted in both aqueous and vacuum environments to investigate the influence of water on the mechanical properties. The results reveal that unpolymerized peptides behave as brittle materials, while polymerized ones exhibit a significant strain-to-failure after initial failure. Moreover, the strength of cyclic peptides is higher in water compared to a vacuum.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Shuai Wang, Suzanne Scarlata, Nima Rahbar
Summary: The article describes a method that allows for the rapid curing of enzymatic construction materials under ambient conditions using low-power lasers and the incorporation of nanoparticles, supporting on-site manufacturing capability for construction materials.
CELL REPORTS PHYSICAL SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Shuai Wang, Suzanne F. Scarlata, Nima Rahbar
Summary: This paper introduces a novel method to create a negative-emission enzymatic construction material (ECM) with self-healing capabilities, by catalyzing the condensation of CO2 and water to form calcium carbonate crystals. The resulting ECM has high strength and modulus, providing a new direction for developing environmentally friendly and low-cost construction materials.
Editorial Material
Materials Science, Multidisciplinary
Nima Rahbar
Article
Materials Science, Multidisciplinary
Daniel R. Williams, Daniel J. Nurco, Nima Rahbar, Kristie J. Koski