Article
Chemistry, Analytical
Goncalo J. Gouveia, Amanda O. Shaver, Brianna M. Garcia, Alison M. Morse, Erik C. Andersen, Arthur S. Edison, Lauren M. McIntyre
Summary: The study developed stable and sustainable reference materials (RM) using the iterative batch averaging method (IBAT), which was then applied in a metabolomics experiment to estimate the proportion of sample variation.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Trevor B. Romsdahl, Jean-Christophe Cocuron, Mackenzie J. Pearson, Ana Paula Alonso, Kent D. Chapman
Summary: This study introduces a stepwise targeted lipidomics approach to characterize polar and non-polar lipid classes. By using hydrophilic interaction chromatography and reverse phase chromatography, changes among different lipid molecular species were found, and potential metabolic explanations were discussed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Vincent Dufour, Laure Wiest, Sylvain Slaby, Francois Le Cor, Lucile Auger, Olivier Cardoso, Laurence Curtet, Laure Pasquini, Xavier Dauchy, Emmanuelle Vulliet, Damien Banas
Summary: Monitoring pesticides in small organisms in the environment requires sensitive analytical methods. This study developed a miniaturized extraction protocol for small biotic samples, allowing for the characterization of multiple pesticides and transformation products. The method showed high extraction rates and recoveries, making it an effective tool for analyzing polar pesticides in small organisms.
Article
Chemistry, Applied
Jose L. Hidalgo-Ruiz, Roberto Romero-Gonzalez, Jose Luis Martinez Vidal, Antonia Garrido Frenich
Summary: A single method was developed for determining polar pesticides and environmental contaminants in edible oils and nuts. The method included optimized extraction processes, clean-up steps, and analysis using LC-QqQ-MS/MS. The study successfully detected phosphonic acid in nuts at concentrations up to 4.6 mg kg(-1).
Review
Chemistry, Analytical
Artemis Lioupi, Maria Marinaki, Christina Virgiliou, Olga Begou, Helen Gika, Ian Wilson, Georgios Theodoridis
Summary: Metabolomics is an interdisciplinary field with applications in various areas, involving the analysis of metabolites in pathways such as glycolysis, amino acid metabolism, and the Krebs cycle. The determination of polar metabolites poses major challenges for chromatographic analysis, and existing methods are unable to comprehensively profile the polar metabolome. This review summarizes current approaches, advances, and trends in using liquid chromatography/mass spectrometry to address the instrumental and intellectual challenges involved in mapping the polar metabolome.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Siok-Geok Chong, Intan S. Ismail, Amalina Ahmad Azam, Shih-Jen Tan, Khozirah Shaari, Jen-Kit Tan
Summary: This study investigated the chemical profiles of non-organic and organic soybeans, and the changes in metabolites after fermentation. The results showed no significant difference in the chemical profiles between non-organic and organic soybeans. However, the metabolite profile of unfermented soybeans shifted to higher amino acid and organic acid content after fermentation, potentially enhancing their nutritional value.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Chemistry, Analytical
Sarju Adhikari, Jingzhi Tian, Abu M. Rustum, Boehringer Ingelheim
Summary: A comprehensive study on the degradation profile of firocoxib was conducted through force degradation studies, revealing its major degradation products and potential degradation pathways. The drug substance was subjected to various stress conditions, and six major degradation products were observed. The results provide valuable information for quality monitoring, stability profiling, and formulation development.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Emrah Gormez, Ozgur Golge, Bulent Kabak
Summary: A study quantifying six polar residues in pomegranate samples from Turkey found that phosphonic acid and fosetyl-Al were detected, with some samples exceeding the EU MRL for fosetyl-Al. Other polar residues were not detected in any samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
Joao P. P. Vieira, Filip Ottosson, Amra Jujic, Vladimir Denisov, Martin Magnusson, Olle Melander, Joao M. N. Duarte
Summary: Combining LC-MS and NMR techniques improves the discrimination of metabolic disease development, including those induced by a high-fat diet. The study confirms that metabolic pathways in mice affected by high-fat diet also impact diabetes in humans.
Article
Food Science & Technology
Ozgur Golge
Summary: A novel and sensitive chromatography method using modified QuPPe sample preparation was validated for determination of eight high polar pesticides in cherries. The method showed good linearity, low residuals, and average recoveries ranging from 70.2 to 105.1%. No high polar pesticides were found in 225 real samples of cherries analyzed using this method.
FOOD ANALYTICAL METHODS
(2021)
Article
Food Science & Technology
Bohai Li, Fan Hui, Zhang Yuan, Qingxiang Shang, Guo Shuai, Yingshu Bao, Yongfu Chen
Summary: This study aimed to investigate the biochemical mechanism of the lipid-lowering effect of consuming koumiss daily by analyzing the fecal metabolomes of hyperlipidemia patients before and after koumiss treatment. Ten significant differential metabolites were detected, showing increased concentrations at days 30 and 60, many of which were involved in lipid metabolism and transport. The metabolites identified were likely associated with the observed lipid-lowering effect.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Review
Chemistry, Analytical
Muhammad Waris, Engin Kocak, Ekrem Murat Gonulalan, L. Omur Demirezer, Sedef Kir, Emirhan Nemutlu
Summary: Plant-based metabolomics studies aim to discover new active compounds, determine vegetation period, investigate adulteration, evaluate correlation activity and metabolite levels, and monitor quality control of medicinal plants. However, the chemical differences of primary and secondary metabolites in complex natural products present challenges for analysis, ranging from sample collection to preparation, and from analysis to metabolite annotation and quantification. A comprehensive evaluation of current and new trends in plant metabolomics helps researchers in designing their studies holistically.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Environmental Sciences
Aikaterini A. Zompra, Styliani A. Chasapi, Matthew S. Twigg, Karina Salek, Ioannis Anestopoulos, Alex Galanis, Aglaia Pappa, Tony Gutierrez, Ibrahim M. Banat, Roger Marchant, Stephen R. Euston, Mihalis I. Panayiotidis, Georgios A. Spyroulias
Summary: Synthetic surfactants, commonly used in various industries, have a negative impact on the environment due to their synthetic preparation. As a result, there is a growing interest in exploring natural sources of surfactants, which can be produced through environmentally friendly processes. Microbial production of such chemicals, however, often results in crude materials composed of multiple complex bioproducts. Therefore, advanced analytical methods are required for the extraction, identification, and characterization of biosurfactants.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemical Research Methods
Yong Lu, Yu He, Xinran Wang, Haiyan Wang, Qianqian Qiu, Baojin Wu, Xianfu Wu
Summary: Recently, new types of illegal cosmetic additives have emerged in the market. These additives are often new drugs or analogues with similar structures to prohibited additives, making them difficult to identify solely by LC-MS. To address this, a new strategy combining chromatographic separation with NMR structural identification is proposed. Suspected samples were screened using UPLC-Q-TOF-MS, followed by purification and extraction through silica-gel column chromatography and preparative HPLC. The extracts were then identified using NMR as bimatoprost and latanoprost, which were found to be new illegal additives in eyelash serums in China. Furthermore, bimatoprost and latanoprost were quantified using HPLC-QQQ-MS/MS, demonstrating good linearity, accuracy, precision, and reproducibility.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Atiah H. Almalki
Summary: Lung cancer is the leading cause of cancer-related death worldwide. This review presents recent trends in the analytical techniques used for metabolome mapping of lung cancer, and discusses the potential applications of these techniques in the analysis of biological samples, providing new avenues for treatment strategies and monitoring drug responses.
Article
Environmental Sciences
R. Almeda, R. Rodriguez-Torres, S. Rist, M. H. S. Winding, P. Stief, B. H. Hansen, T. Gissel Nielsen
Summary: The study revealed that Arctic sea ice has high concentrations of microplastics, and the reduction of sea ice in the Arctic opens up new opportunities for the oil and maritime industries, increasing the risk of oil pollution in the region. Copods exposed to microplastics and crude oil may exhibit behavioral stress responses, such as feeding suppression.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Raymond Nepstad, Bjorn Henrik Hansen, Jorgen Skancke
Summary: Offshore oil and gas platforms discharge produced water containing PAHs into the ocean, impacting the early life stages of Atlantic Cod in the North Sea. A numerical model was used to study the fate and uptake of PAHs, showing significant differences between predicted internal concentrations and discharge concentrations of PAHs over multiple years of simulation.
MARINE ENVIRONMENTAL RESEARCH
(2021)
Article
Environmental Sciences
Trond Nordtug, Anders J. Olsen, Per-Arvid Wold, Iurgi Salaberria, Ida Beathe overjordet, Dag Altin, Elin Kjorsvik, Bjom Henrik Hansen
Summary: This study demonstrates that the timing of exposure and the presence of oil micro droplets can affect the uptake and survival of PAH in pelagic Atlantic cod eggs exposed to oil dispersions. Although the dissolved fraction is the main driver of toxicity in cod embryos, crude oil micro droplets also contribute to increased mortality.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Sofia Soloperto, Dag Altin, Anna Hallmann, Elise Skottene, Bjorn H. Hansen, Bjorn M. Jenssen, Tomasz M. Ciesielski
Summary: The copepod Calanus finmarchicus responds to deep-sea petroleum pollution mainly through lipid peroxidation, with significantly higher glutathione S-transferase activity and malondialdehyde concentrations in the exposed group compared to the control group.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Fisheries
Arne M. M. Malzahn, Antonio Sarno, Andreas Hagemann, Julia Farkas, Luciana Alves Musialek, Elin Kjorsvik, Bjorn Henrik Hansek
Summary: This study analyzed the egg quality of ballan wrasse and identified lipid species that are related to physiological and morphological egg quality markers. These lipid species can be proposed as key indicators for selecting high quality egg batches in hatcheries in the aquaculture industry.
Article
Environmental Sciences
Bjorn Henrik Hansen, Trond Nordtug, Ida Beathe Overjordet, Dag Altin, Julia Farkas, Per S. Daling, Kristin Rist Sorheim, Liv-Guri Faksness
Summary: This study assessed the potential toxicity of chemical herders to cold-water marine organisms. The results showed that herders are relatively non-toxic and do not significantly increase the toxicity when mixed with oil.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Oscar Velasco-Rodriguez, Mariana Fil, Tonje M. B. Heggeset, Kristin F. Degnes, David Becerro-Recio, Katarina Kolsakova, Tone Haugen, Malene Jonsson, Macarena Toral-Martinez, Carlos Garcia-Estrada, Alberto Sola-Landa, Kjell D. Josefsen, Havard Sletta, Carlos Barreiro
Summary: This study found that bacterial diversity in decaying wood was higher than fungal heterogeneity, with 194 different cultivable bacterial isolates mainly belonging to Bacillaceae, Streptomycetaceae, Paenibacillaceae, and Microbacteriaceae, and 343 fungal strains mainly belonging to Aspergillaceae, Hypocreaceae, and Coniochaetaceae. The results also suggest that decaying wood is an ecologically rich niche and a promising source of biotechnologically interesting microorganisms.
Article
Fisheries
Bjorn Henrik Hansen, Elin Kjorsvik, Arne M. Malzahn, Antonio Sarno, Ole Martin Kulild, Julia Farkas, Trond Nordtug, Rebecca Rye, Bjarne Kvaestad, Ingrid Lein, Andreas Hagemann
Summary: Wild-caught lumpfish have higher egg quality and hatching success compared to captive lumpfish, with no significant differences in other biological variables. The lipid composition of lumpfish eggs can be used to differentiate between eggs from wild-caught females of different origins and captive broodstock with different diets, and these lipid profiles can also predict the quality of the eggs.
Article
Environmental Sciences
Bjorn Henrik Hansen, Trond Nordtug, Ida Beathe Overjordet, Lisbet Sorensen, Bjarne Kvaestad, Emlyn John Davies, Sonnich Meier, Tania Gomes, Steven Brooks, Julia Farkas
Summary: Fish embryos can be used as sentinels for environmental biomonitoring, but their fragility and seasonality limit their use. In this study, caged lumpfish eggs were successfully used for in situ biomonitoring of organic contaminants. The eggs showed high survival rates in different environments. Organic contaminants were effectively accumulated in eggs in polluted areas, but no evidence of toxicity was found in terms of larval morphometry.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Laura Garcia-Calvo, Raquel Rodriguez-Castro, Ricardo Ullan, Silvia M. Albillos, Marta Fernandez-Aguado, Claudia M. Vicente, Kristin F. Degnes, Havard Sletta, Carlos Barreiro
Summary: This study identified a potential lignocellulolytic enzyme producer, Penicillium rubens, which overexpressed two genes (PrFaeA and PrFaeB) with feruloyl esterase (FAE) activity. Enzyme extracts from PrFaeB-enriched cultures showed optimal FAE activity at pH 5.0 and 50-55 degrees C, as confirmed by proteome analysis and mass spectrometry. The study also analyzed other lignocellulolytic activities, untargeted metabolites, and scaled up FAE production in stirred tank reactors. These findings highlight the potential of P. rubens as a promising lignocellulolytic enzyme producer.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Fisheries
Arne M. Malzahn, Deni Ribicic, Bjorn Henrik Hansen, Antonio Sarno, Elin Kjorsvik, Anna Sigrid Norberg Aase, Luciana Alves Musialak, Laura Garcia-Calvo, Andreas Hagemann
Summary: The use of cleaner fish, specifically ballan wrasse, is crucial for controlling sea lice in salmonid cage farming. However, large-scale cultivation of ballan wrasse faces challenges due to the difficulty of the initial feeding phase and the lack of feeding protocols. In an experiment, feeding larvae copepods as their first diet resulted in higher survival rates and better growth compared to other feeding regimes.
Article
Biotechnology & Applied Microbiology
Susann Schulz, Havard Sletta, Kristin Flogstad Degnes, Sergii Krysenko, Alicia Williams, Silje Malene Olsen, Kai Vernstad, Agnieszka Mitulski, Wolfgang Wohlleben
Summary: FK-506 is a potent immunosuppressive macrocyclic polyketide produced by Streptomyces tsukubaensis, but its biotechnological production is limited due to low levels synthesized by the wild-type strain. This study investigated the global regulatory aspects of FK-506 biosynthesis, particularly focusing on the role of the Crp regulator. Crp overexpression led to a two-fold increase in FK-506 production, which was successfully transferred to industrial scale using fermentors.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Lisbet Sorensen, Julia Farkas, Ida Beathe Overjordet, Bjorn Henrik Hansen
Summary: This study demonstrates the migration and potential harm of plastics- and rubber-derived chemicals in the environment. A novel biomonitoring platform using caged lumpfish eggs combined with gas chromatography tandem mass spectrometry analysis was used to detect the target chemicals. Monitoring in Trondheim harbor and off the coast of Trondelag in Norway revealed the accumulation of various chemicals in deployed eggs.
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
(2023)
Article
Multidisciplinary Sciences
David R. Williamson, Trond Nordtug, Frode Leirvik, Bjarne Kvaestad, Bjorn Henrik Hansen, Martin Ludvigsen, Emlyn John Davies
Summary: Microscopic imaging and morphometric measurement of fish embryos and larvae are crucial for environmental monitoring and aquaculture. Our approach allows for fast imaging and analysis of millimetre-scale ichthyoplankton, producing results comparable to traditional microscopy while saving time and effort.
Article
Multidisciplinary Sciences
Bjarne Kvaestad, Bjorn Henrik Hansen, Emlyn Davies
Summary: Measurement of morphometric parameters on fish larvae is important for environmental research and cultivation industry. The use of Mask R-CNN for instance segmentation helps to automate parameter acquisition, saving time and improving accuracy. This technique has been successfully tested on various species of organisms.
