Article
Virology
Jessica Morel, Laura Sedano, Nathalie Lejal, Bruno Da Costa, Eric Batsche, Christian Muchardt, Bernard Delmas
Summary: The interaction between the N-third domain of PB2 and RPB4 plays a critical role in the cap-snatching activity of FluPol during influenza virus transcription. This study suggests that FluPol recognizes multiple domains of RNA polymerase II to ensure efficient cap-snatching and transcription of viral genomic RNA.
Article
Biochemistry & Molecular Biology
Karli A. Lipinski, Jing Chi, Xin Chen, Aaron A. Hoskins, David A. Brow
Summary: U6 small nuclear RNA (snRNA) is a highly conserved component of spliceosomes. It has been traditionally thought that U6 snRNA is synthesized by RNA polymerase III (RNAP III). However, this study demonstrates that U6 snRNA can also be synthesized by RNA polymerase II (RNAP II). The ability to synthesize U6 snRNA with RNAP II provides more flexibility in controlling U6 levels. Furthermore, the formation of U6 snRNPs containing the Sm complex suggests that U6 snRNA synthesized by RNAP II is functional in vivo.
Article
Cell Biology
Stephen Richard, Lital Gross, Jonathan Fischer, Keren Bendalak, Tamar Ziv, Shira Urim, Mordechai Choder
Summary: Rpb4/7 binds RNA Pol II transcripts co-transcriptionally and regulates mRNA life cycle stages by undergoing over 100 combinations of post-translational modifications. Temporal PTMs of Rpb4/7 mediate interactions with key regulators, impacting mRNA synthesis and decay balance, as well as the cell's response to the environment.
Article
Biochemistry & Molecular Biology
Izabela Rudzinska, Malgorzata Ciesla, Tomasz W. Turowski, Alicja Armatowska, Ewa Lesniewska, Magdalena Boguta
Summary: The study showed that the rpc128-1007 mutant disrupts Pol III assembly, affecting mRNA expression levels, particularly through modulation of the general transcription factor Gcn4. Additionally, regardless of suppressor genes, the rpc128-1007 mutation leads to a slight increase in the expression of ribosome biogenesis genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Laura J. Hsieh, Muryam A. Gourdet, Camille M. Moore, Elise N. Munoz, Nathan Gamarra, Vijay Ramani, Geeta J. Narlikar
Summary: The INO80 chromatin remodeling complex plays a critical role in transcription by preferentially mobilizing hexasomes over nucleosomes. It does so by dislodging an H2A-H2B dimer, allowing nucleosomes to transiently resemble hexasomes. This versatile mechanism allows INO80 to regulate chromatin architecture during transcription, replication, and repair.
Article
Biochemistry & Molecular Biology
Tao Wang, Yumei Chang, Kai Zhao, Qing Dong, Jun Yang
Summary: Loss of RCL1 impairs 18S pre-rRNA processing and translation efficiency, leading to abnormal embryo differentiation and storage reserve synthesis during maize kernel development.
Article
Cell Biology
Jia Fei, Jun Xu, Ziwei Li, Kevin Xu, Dong Wang, George A. Kassavetis, James T. Kadonaga
Summary: NDF is a transcription elongation factor that binds to Pol II and enhances gene expression elongation by a different mechanism than TFIIS.
GENES & DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
A. I. Garrido-Godino, I. Gupta, F. Gutierrez-Santiago, A. B. Martinez-Padilla, A. Alekseenko, L. M. Steinmetz, J. E. Perez-Ortin, V. Pelechano, F. Navarro
Summary: The study identified the molecular mechanisms mediated by Rpb4 in regulating posttranscriptional events of mRNA imprinting and stability. Rpb4 interacts with a large population of mRNAs and collaborates with Puf3 to affect the stability and imprinting of a common group of mRNAs. This suggests that Rpb4 may play a key role in coordinating mRNA synthesis, imprinting, and stability in cooperation with RNA binding proteins.
Article
Biochemistry & Molecular Biology
Mahtab Peyambari, Shengxi Guan, Marilyn J. Roossinck
Summary: Experimental findings show that RdRp proteins of partitiviruses have reverse transcriptase (RT) function, can be active at lower temperatures, sparking researchers to question why these viruses have RT capabilities.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Multidisciplinary Sciences
Trevor van Eeuwen, Tao Li, Hee Jong Kim, Jose J. Gorbea Colon, Mitchell Parker, Roland L. Dunbrack, Benjamin A. Garcia, Kuang-Lei Tsai, Kenji Murakami
Summary: During transcription initiation, TFIIH marks RNA polymerase II by phosphorylating Ser5 of Rpb1's CTD, followed by modifications related to transcription elongation, mRNA processing, and histone dynamics. The structure of TFIIK helps extend the proposed model of the CTD path to TFIIH's active site.
Article
Biology
Shu-Hao Liou, Sameer K. Singh, Robert H. Singer, Robert A. Coleman, Wei-Li Liu
Summary: Liou et al. report a 4.6 angstrom resolution structure of the human p53/RNA polymerase II assembly, using single particle cryoelectron microscopy. This study suggests that p53's functional domains regulate the DNA binding activity of RNA polymerase II, providing insights into p53-regulated gene expression.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ana I. Garrido-Godino, Abel Cuevas-Bermudez, Francisco Gutierrez-Santiago, Maria del Carmen Mota-Trujillo, Francisco Navarro
Summary: Rtr1 plays a critical role in RNA pol II assembly and mRNA decay regulation by mediating the association between RNA pol II and Rpb4/7. This study reveals new mechanisms in gene regulation and the crosstalk between mRNA synthesis and decay.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Feilong Chen, Beibei Liu, Hao Zhou, Jiafu Long
Summary: The study reveals a previously uncharacterized multistep pathway in which the Paf1 complex is involved in regulating the RNAPII pool in response to DNA damage. Dephosphorylation of Spt5 weakens the interaction between Rtf1 and Spt5, which is a key step in initiating the degradation of Rpb1. Rad26 is loaded onto stalled RNAPII to replace the Spt4/Spt5 complex and recruits more PAF1C to DNA lesions, leading to RNAPII removal. The intact PAF1C coordinates with Rad26 to localize the Elongin-Cullin complex on stalled RNAPII, thereby inducing RNAPII removal.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Ruth Q. Jacobs, Kaila B. Fuller, Stephanie L. Cooper, Zachariah Carter, Marikki Laiho, Aaron L. Lucius, David A. Schneider
Summary: This study evaluates the specificity of the compound BMH-21 on transcription by Pols I, II, and III. The results show that Pol I is more sensitive to inhibition by BMH-21 compared to Pols II and III. These findings support the ongoing development of BMH-21 and its derivatives as potential therapeutic agents.
Article
Multidisciplinary Sciences
Sandra Schilbach, Haibo Wang, Christian Dienemann, Patrick Cramer
Summary: This study presents an atomic model for the yeast preinitiation complex (PIC) with core mediator, revealing the molecular details of RNA polymerase II (Pol II) transcription initiation. The structure of the mediator middle module was optimized, and the missing subunit Med1 was identified. The study found that the flexible C-terminal repeat domain (CTD) of Pol II forms defined interactions with the mediator head and middle modules, as well as other regions of the mediator. The binding mode between the yeast mediator and Pol II differs from the human counterpart.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)