Article
Chemistry, Analytical
Bolin Jing, Lei Yan, Jiajia Li, Piaopiao Luo, Xiaoni Ai, Pengfei Tu
Summary: A high-throughput human renal proximal tubule model based on an integrated biomimetic array chip (iBAC) demonstrated improved epithelial polarization and membrane transporter activity, offering a more accurate prediction of nephrotoxicity.
Review
Cell Biology
Min Chen, Xiangchen Gu
Summary: Endocytosis plays a crucial role in kidney diseases, particularly in renal fibrosis, and is affected by other diseases and factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Urology & Nephrology
Lihe Chen, Chung-Lin Chou, Chin-Rang Yang, Mark A. Knepper
Summary: This study investigated sex differences in kidney physiology and pathophysiology using RNA-seq, ATAC-seq, and protein mass spectrometry methods. The results revealed significant differences in gene expression and chromatin accessibility in the proximal tubules between males and females. Many of the differentially expressed genes are involved in metabolic processes and transport functions. The study also identified several transcription factors and proteins that contribute to sex differences. The findings provide valuable insights into the regulatory mechanisms underlying sex differences in kidney function.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Physiology
Molly A. Bingham, Kim Neijman, Chin -Rang Yang, Angel Aponte, Angela Mak, Hiroaki Kikuchi, Hyun Jun Jung, Brian G. Poll, Viswanathan Raghuram, Euijung Park, Chung -Lin Chou, Lihe Chen, Jens Leipziger, Mark A. Knepper, Margo Dona
Summary: The circadian variability in kidney function is often ignored in physiological experiments. This study used transcriptomics and proteomics to identify genes and proteins in the kidney that show circadian rhythms. These data have been made available as user-friendly web resources.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Cell Biology
Shiting Liang, Youliang Wang, Meixia Kang, Juan Deng, Liting Chen, Xizhen Hong, Fan Fan Hou, Fujian Zhang
Summary: Protein reabsorption in renal proximal tubules is crucial for maintaining nutrient balance. We developed a new mouse model, AMN (CreERT2) knock-in mice, which express a fusion protein of Cre recombinase and estrogen receptor under the control of the AMN gene promoter specifically in renal proximal tubules. This model allows for the conditional knockout of genes in renal proximal tubules, providing valuable insights into their physiological function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Chemistry, Medicinal
Muyao Ye, Ming Yang, Wenni Dai, Hao Li, Xun Zhou, Yinyin Chen, Liyu He
Summary: Obesity-related glomerulopathy (ORG) is a secondary kidney damage caused by obesity, and there is currently a lack of effective strategies to prevent and delay its development. This article reviews the pathological characteristics of ORG, describes the roles of lipid metabolism disorders and mitochondrial oxidative stress in its development, and suggests potential therapeutic targets for ORG treatment.
Article
Biochemistry & Molecular Biology
Ramkumar Thiyagarajan, Mary Taub
Summary: Cystinosis is a genetic disease that causes renal failure. Study using human-induced pluripotent stem cells found that the differentiation ability of cystinotic cells is impaired, which may lead to the malfunctioning of renal proximal tubule.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shreya T. Mukherji, Luca Brambilla, Kailey B. Stuart, Isabella Mayes, Laura C. Kutz, Yiliang Chen, Leandro A. Barbosa, Ibrahim Elmadbouh, Jeff P. McDermott, Steven T. Haller, Michael F. Romero, Manoocher Soleimani, Jiang Liu, Joseph I. Shapiro, Gustavo V. Blanco, Zijian Xie, Sandrine V. Pierre
Summary: Through ATP-dependent ion pumping, basolateral Na/K-ATPase (NKA) generates a Na+ gradient that drives apical Na+ reabsorption in the renal proximal tubule (RPT), while NKA signaling function triggers cellular redistribution and decreases Na+ reabsorption. Knockdown of RPT NKA leads to increased membrane NHE3 and NBCe1A, resulting in decreased urine output and Na+ excretion, elevated blood pressure, and increased RPT Na+ reabsorption. NKA signaling plays an important role in regulating Na+ reabsorption in RPT, functioning dominantly over NKA ion pumping.
Review
Cell Biology
Zahraa S. Hotait, Julia N. Lo Cascio, Elijah N. D. Choos, Blythe D. Shepard
Summary: The renal proximal tubule plays a crucial role in solute reabsorption and glucose metabolism. Understanding these processes helps unravel the mechanisms underlying renal diseases and explore potential therapeutic approaches.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Physiology
Hao Liu, Qi Sun, Zheng Ding, Wensen Shi, Wen-Hui Wang, Chengbiao Zhang
Summary: This study found that adenosine activates the basolateral K+ channels in the proximal tubules of mice through adenosine A1 receptor, and the mechanism of action is mediated by the PLC-PKC signaling pathway.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Medicine, General & Internal
Xiaohong Xiang, Jiefu Zhu, Gang Zhang, Zhengwei Ma, Man J. Livingston, Zheng Dong
Summary: The study demonstrates that p53 plays a critical role in kidney injury associated with cold storage/transplantation, and inhibiting p53 can improve the histology and function of transplanted kidneys, showing potential as a therapeutic target for kidney transplantation.
FRONTIERS IN MEDICINE
(2021)
Article
Medical Laboratory Technology
Ali C. M. Johnson, Richard A. Zager
Summary: Cardiorenal syndrome I (CRS-1) is a state of acute kidney injury in acute heart failure (AHF), induced by isoproterenol (Iso) in mouse model showed iron-mediated cortical abnormalities with oxidative stress and pro-inflammatory reactions, despite no lasting renal injury. Iso affects both hemodynamics and tubular cell toxicity, potentially contributing to a systemic inflammatory state through renal cytokine production.
TRANSLATIONAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sindhu Naik, Andrew R. Wood, Mate Ongenaert, Paniz Saidiyan, Edo D. Elstak, Henriette L. Lanz, Jan Stallen, Richard Janssen, Elizabeth Smythe, Kai S. Erdmann
Summary: Research demonstrates that in a proximal tubule organ on chip model replicating characteristics of Lowe syndrome/Dent II disease, proximal tubule cells lacking OCRL expression upregulate markers associated with epithelial-mesenchymal transition (EMT) and exhibit increased collagen expression and deposition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Marianne K. Vormann, Jelle Vriend, Henriette L. Lanz, Linda Gijzen, Angelique van den Heuvel, Simon Hutter, Jos Joore, Sebastiaan J. Trietsch, Christiaan Stuut, Tom T. G. Nieskens, Janny G. P. Peters, Daniela Ramp, Michaela Caj, Frans G. M. Russel, Bjoern Jacobsen, Adrian Roth, Shuyan Lu, Joseph W. Polli, Anita A. Naidoo, Paul Vulto, Rosalinde Masereeuw, Martijn J. Wilmer, Laura Suter-Dick
Summary: The Nephroscreen platform, using a combination of cell viability, LDH, and miRNA release, proved to be a robust and predictive method for detecting drug-induced kidney injury. It is compatible with automated pipetting, suitable for long-term experiments, and offers a valuable tool for replacing animal testing and supporting the 3Rs principles.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Benjamin Koch, Dominik C. Fuhrmann, Ralf Schubert, Helmut Geiger, Thimoteus Speer, Patrick C. Baer
Summary: Inflammation is closely related to the development of diabetic kidney disease. Gliflozins, as SGLT-2 inhibitors, have been shown to reduce inflammation and slow down the progression of diabetic nephropathy. In this study using human renal proximal tubular epithelial cells, gliflozins were found to have an anti-inflammatory effect by inhibiting the expression of pro-inflammatory factors and the adhesion molecule ICAM-1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)