In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

Mapping DNA polymerase errors by single-molecule sequencing

(2016) David F. Lee et al.   NUCLEIC ACIDS RESEARCH

Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost

(2016) Roger M. Benoit et al.   PLoS One

REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering

(2016) Ashutosh Trehan et al.   Scientific Reports

IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly

(2016) Javier García-Nafría et al.   Scientific Reports

Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer

(2015) Edward M. Kennedy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli

(2015) Ana Paula Jacobus et al.   PLoS One

Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

(2015) Maxim Kostylev et al.   PLoS One

A universal cloning method based on yeast homologous recombination that is simple, efficient, and versatile

(2014) Tammy M. Joska et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Rapid assembly of multiple DNA fragments through direct transformation of PCR products into E. coli and Lactobacillus

(2014) Pinghua Cao et al.   PLASMID

Total Synthesis of a Functional Designer Eukaryotic Chromosome

(2014) N. Annaluru et al.   SCIENCE

SLiCE: a novel bacterial cell extract-based DNA cloning method

(2012) Yongwei Zhang et al.   NUCLEIC ACIDS RESEARCH

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

(2011) Chaokun Li et al.   BMC BIOTECHNOLOGY

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids

(2010) Anton Bryksin et al.   BIOTECHNIQUES

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

(2008) D. G. Gibson et al.   SCIENCE