Article
Cell Biology
Marcin Czop, Karolina Gasinska, Ewa Kosior-Jarecka, Dominika Wrobel-Dudzinska, Janusz Kocki, Tomasz Zarnowski
Summary: The expression levels of miRNA in the aqueous humor of PEXG patients were evaluated using expression microarray method. Twenty new miRNA molecules potentially associated with the development or progression of PEXG were identified. Functional and enrichment analysis revealed that these miRNAs may regulate mechanisms related to extracellular matrix imbalance, cell apoptosis (possibly retinal ganglion cells), autophagy, and elevated calcium cation levels in PEXG. However, further research is needed to understand the exact molecular basis of PEXG.
Article
Multidisciplinary Sciences
Prity Sahay, Shweta Reddy, Birendra Kumar Prusty, Rahul Modak, Aparna Rao
Summary: The study found that in PXG patients, the protein expression of TGF beta 1, MMP-9, and FN1 was upregulated in tears, tenon's capsule, and AH samples, while MMP-9 protein activity was downregulated. Additionally, certain cytokines showed significant differences between PXG and PXF, suggesting that alterations in the expression of these molecules in tears could serve as a signal for the onset of glaucoma.
Article
Cell Biology
Karolina Gasinska, Marcin Czop, Ewa Kosior-Jarecka, Dominika Wrobel-Dudzinska, Janusz Kocki, Tomasz Zarnowski
Summary: In this study, we analyzed the levels of snoRNA expression in the aqueous humor of patients with pseudoexfoliation glaucoma (PEXG) and identified potential snoRNA-dependent mechanisms contributing to the disease. We found significantly different expression levels of seven snoRNAs in the PEXG and control groups. Five snoRNAs were downregulated in the PEXG group, while two snoRNAs were upregulated. We also discovered similarities between PEX-related genes and factors associated with two snoRNAs.
Article
Ophthalmology
Fuat Yavrum, Ufuk Elgin, Zeynep Adiyaman Kocer, Vildan Fidanci, Emine Sen
Summary: The study compared the clusterin levels in aqueous humor and serum of PEX, PEXG, POAG patients, and controls. The study found higher clusterin levels in the anterior chamber of PEXG patients, with a possible association in POAG as well.
Article
Ophthalmology
Jieying Guan, Zhidong Li, Aizezi Wumaier, Yuncheng Ma, Lingling Xie, Heping Wu, Rongxin Chen, Yingting Zhu, Yehong Zhuo
Summary: This study investigated the role of N6-methyladenosine (m6A) modification in the pathogenesis of pseudoexfoliation glaucoma (PXG). The researchers found significantly higher levels of m6A in the aqueous humor of PXG patients compared to patients with age-related cataract (ARC). They also identified upregulated m6A-related enzymes and observed m6A-modified gene transcripts related to extracellular matrix formation and histone deacetylation. The results suggest that m6A methylation may regulate extracellular matrix composition in PXG, with METTL3 playing a pivotal role.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Diana Anna Dmuchowska, Karolina Pietrowska, Pawel Krasnicki, Tomasz Kowalczyk, Magdalena Misiura, Emil Tomasz Grochowski, Zofia Mariak, Adam Kretowski, Michal Ciborowski
Summary: Pseudoexfoliation syndrome (XFS) is a stress- or inflammation-induced elastosis that affects approximately 60-70 million people worldwide, with its pathogenesis not fully elucidated. Analysis of aqueous humor from XFS patients undergoing cataract surgery revealed lower levels of amino acids and derivatives, along with higher levels of certain metabolites.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Ophthalmology
Do Young Park, Moohyun Kim, Soon Cheol Cha
Summary: Both TGF-beta and inflammatory cytokines were highly expressed in the aqueous humor of patients with exfoliation glaucoma, potentially playing a role in intraocular pressure elevation in XFG.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Health Care Sciences & Services
Karny Shouchane-Blum, Alon Zahavi, Noa Geffen, Yoav Nahum, Eitan Livny, Irit Rosenblatt, Ruti Sella, Irit Bahar, Amir Sternfeld, Dan Gaton
Summary: This study aimed to investigate the effect of cataract extraction on the detection of pseudoexfoliation syndrome (PXF) by ophthalmologists. A comparative study was conducted on 31 patients undergoing cataract surgery. Pre-operatively, 12 patients were diagnosed with PXF, and post-operatively, the diagnosis of PXF did not differ significantly. However, the detection of anterior capsular deposits, Sampaolesi lines, and pupillary ruff deposits were significantly lower after surgery.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Medicine, General & Internal
Carlos Oribio-Quinto, Barbara Burgos-Blasco, Pilar Perez-Garcia, Laura Espino-Paisan, Beatriz Sarria, Jose Ignacio Fernandez-Vigo, Julian Garcia-Feijoo
Summary: This study compared the cytokine concentrations in the aqueous humor of patients with primary congenital glaucoma (PCG), primary open-angle glaucoma (POAG), and a control group. It was found that PCG patients had lower concentrations of multiple cytokines in their aqueous humor compared to POAG patients and those without glaucoma.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Ophthalmology
Kexin Li, Xue Feng, Kenji Hikosaka, Kazumi Norose
Summary: A murine model of primary acquired ocular toxoplasmosis was successfully established through peroral infection with Toxoplasma gondii. After infection, various immune mediators in the aqueous humor showed significant increase, providing new insights into the immunopathogenesis of ocular toxoplasmosis.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Hyun-Kyung Cho, Hyemin Seong, Changwon Kee, Dae Hyun Song, Seong Jae Kim, Seong Wook Seo, Sang Soo Kang
Summary: By analyzing the levels of different miRNAs in aqueous humor samples, we found differences in miRNA expression between PEX glaucoma and NTG patients, suggesting a possible role of miRNAs in the pathogenesis of glaucoma and implying different mechanisms for different types of glaucoma.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Peixing Wan, Erping Long, Zhidong Li, Yingting Zhu, Wenru Su, Yehong Zhuo
Summary: Glaucoma, affecting over 70 million individuals worldwide, is primarily caused by circulatory disturbances of aqueous humor. A study identified GDF7 hypomethylation as a crucial factor in the onset of AH outflow disorders in glaucoma patients. GDF7 neutralization therapy was developed to inhibit TM fibrosis and significantly improve outflow facility, controlling IOP and protecting nerve fibers.
Article
Oncology
Afsaneh Naderi Beni, Zahra Imani, Heshmatollah Ghanbari
Summary: The study found significant differences in OCT-A parameters among normal and glaucomatous eyes, with significant differences in peripapillary VD and macular vessel density between POAG and PXG eyes. PXG eyes exhibited more severe damage in superficial and deep macular vessel densities.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Anton W. Roodnat, Breedge Callaghan, Chelsey Doyle, Megan Henry, Katarzyna Goljanek-Whysall, David A. Simpson, Carl Sheridan, Sarah D. Atkinson, Colin E. Willoughby
Summary: Pseudoexfoliation glaucoma is an aggressive disease that affects about 30 million people worldwide. TGF-beta 1 plays a role in its pathogenesis. Through RNA sequencing, we identified several differentially expressed genes and determined important pathways and molecular functions. This cellular model provides insights into the disease mechanism and supports the development of novel therapies.
Review
Medicine, General & Internal
Martyna Tomczyk-Socha, Wojciech Tomczak, Weronika Winkler-Lach, Anna Turno-Krecicka
Summary: Pseudoexfoliation syndrome (XFS) is a condition characterized by the accumulation of excess material in both the anterior chamber and various body tissues. The frequency of XFS varies significantly depending on location and examination method. Environmental risk factors include exposure to sunlight, proximity to the equator, dietary factors, alcohol consumption, UV exposure, and outdoor work. XFS can be identified by the presence of white material on the lens capsule and other anterior chamber components, as well as the presence of a Sampaolesi line during gonioscopy. Systemic alterations indicative of XFS have been observed in various tissues throughout the body. XFS is the most common cause of secondary open-angle glaucoma, known as pseudoexfoliative glaucoma, which is more severe than primary open-angle glaucoma. The development of pseudoexfoliation syndrome may be influenced by a combination of environmental factors and genetic alterations, but further research is needed.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Ophthalmology
Hamid Ahmadieh, Ramin Nourinia, Ali Hafezi-Moghadam, Hamideh Sabbaghi, Shintaro Nakao, Souska Zandi, Mehdi Yaseri, Zahra Tofighi, Shadi Akbarian
BRITISH JOURNAL OF OPHTHALMOLOGY
(2019)
Article
Ophthalmology
Justus G. Garweg, Souska Zandi, Isabel Pfister, Robert Rieben, Magdalena Skowronska, Christoph Tappeiner
ACTA OPHTHALMOLOGICA
(2019)
Article
Biochemistry & Molecular Biology
Aliaa Barakat, Shintaro Nakao, Souska Zandi, Dawei Sun, Ruth Schmidt-Ullrich, K. C. Hayes, Ali Hafezi-Moghadam
Article
Multidisciplinary Sciences
Souska Zandi, Isabel B. Pfister, Peter G. Traine, Christoph Tappeiner, Alain Despont, Robert Rieben, Magdalena Skowronska, Justus G. Garweg
Article
Ophthalmology
Souska Zandi, Florentina Freiberg, Veronika Vaclavik, Isabel B. Pfister, Peter G. Traine, Cagdas Kaya, Stephan Michels, Justus G. Garweg
JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS
(2020)
Article
Biochemistry & Molecular Biology
Narsis Daftarian, Souska Zandi, Golbarg Piryaie, Mahin Nikougoftar Zarif, Ehsan Ranaei Pirmardan, Muneo Yamaguchi, Qurban Behzadian Nejad, Hossein Hasanpour, Shahram Samiei, Isabel B. Pfister, Zahra-Soheila Soheili, Shintaro Nakao, Aliaa Barakat, Justus G. Garweg, Hamid Ahmadieh, Ali Hafezi-Moghadam
Article
Endocrinology & Metabolism
Simon Leonhard April-Monn, Tabea Wiedmer, Magdalena Skowronska, Renaud Maire, Marco Schiavo Lena, Mafalda Trippel, Annunziata Di Domenico, Francesca Muffatti, Valentina Andreasi, Gabriele Capurso, Claudio Doglioni, Corina Kim-Fuchs, Beat Gloor, Maria Chiara Zatelli, Stefano Partelli, Massimo Falconi, Aurel Perren, Ilaria Marinoni
Summary: This study established a novel in vitro 3-dimensional human primary PanNET culture system as a preclinical model for personalized therapy selection. The platform allows multicenter sample collection and drug screening, showing that primary cells isolated from PanNET patients can form islet-like tumoroids in vitro and display varying therapeutic response profiles. The model is believed to be suitable for personalized drug testing in a larger patient cohort, aiding in identifying novel markers predicting treatment response and refining PanNET therapy.
NEUROENDOCRINOLOGY
(2021)
Article
Ophthalmology
Isabel B. Pfister, Souska Zandi, Christin Gerhardt, Jan Spindler, Natalie Reichen, Justus G. Garweg
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2020)
Article
Cell Biology
Lucia Saucedo, Isabel B. Pfister, Souska Zandi, Christin Gerhardt, Justus G. Garweg
Summary: The study analyzed the concentrations of TGF-beta 1, MMP-3, and TIMP-1 in patients with different stages of diabetic retinopathy (DR), finding significant changes in these cytokines which may play a crucial role in the progression of the disease.
MEDIATORS OF INFLAMMATION
(2021)
Review
Medicine, General & Internal
Arthur Baston, Christin Gerhardt, Souska Zandi, Justus G. Garweg
Summary: Analysis of 14 publications and one case report showed that immediate anti-VEGF treatment after symptom onset in MNV secondary to SFD resulted in favorable responses. Visual acuity worsened in some cases post-treatment, while others improved significantly or remained stable.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Souska Zandi, Isabel B. Pfister, Justus G. Garweg
Summary: The study investigated the relationship between preoperative concentrations of cytokines and chemokines in vitreous fluid and the de novo development of postoperative PVR, revealing CXCL5 as a potential strong biomarker for preventing PVR after surgery.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Lucia Saucedo, Isabel B. B. Pfister, Christin Schild, Souska Zandi, Justus G. G. Garweg
Summary: An imbalance of plasma apolipoproteins has been linked to diabetic retinopathy (DR). This study found that the concentrations of apolipoproteins in the aqueous humor (AH) increased in advancing stages of DR, suggesting their role in the pathogenesis of DR.
MEDIATORS OF INFLAMMATION
(2022)
Article
Ophthalmology
Souska Zandi, Yuebing Li, Laura Jahnke, Anelia Schweri-Olac, Keijiro Ishikawa, Iori Wada, Shintaro Nakao, Martin S. Zinkernagel, Volker Enzmann
Summary: Subretinal fibrosis, which occurs in neovascular age-related macular degeneration (nAMD), leads to progressive deterioration of vision in AMD patients. Intravitreal anti-vascular endothelial growth factor (VEGF) injections can reduce choroidal neovascularization (CNV), but have limited effect on subretinal fibrosis. Therefore, we developed an animal model of subretinal fibrosis without active CNV to investigate the impact of anti-fibrotic compounds on fibrosis.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Ophthalmology
Peter G. Traine, Isabel B. Pfister, Souska Zandi, Jan Spindler, Justus G. Garweg
OPHTHALMOLOGY RETINA
(2019)