Article
Ecology
Xiaojun Lin, Chaonan Zhang, Ruiqi Han, Shoupeng Li, Huishi Peng, Xiao Zhou, Lu Huang, Yanbin Xu
Summary: Horizontal gene transfer (HGT) is the most important pathway for the introduction of antibiotic resistance genes (ARGs), which pose serious threats to human health and biological security. This study developed a visual conjugative model to investigate the potential for HGT and the role of exogenous ARGs in the intestinal microbiome. The results showed that ARG transfer could occur more easily under stressors, and the viability of intestinal bacteria played a key role in conjugation efficiency. Furthermore, the study revealed the mechanisms of ARG transfer under stress and identified the predominant recipient species.
Article
Multidisciplinary Sciences
Varnica Khetrapal, Liyana Ayub Ow Yong, Sylvester J. M. Lim, Swaine L. Chen
Summary: This study introduces the concept of mass allelic exchange (MAE) in Escherichia coli, which allows for classical linkage analysis that connects genotypes to phenotypes. The utility of MAE is demonstrated through virulence-related gain-of-function screens, providing insights into the natural allelic variation in E. coli and potentially other bacteria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Infectious Diseases
Andrew J. Fratoni, Lindsay M. Avery, David P. Nicolau, Tomefa E. Asempa
Summary: This study assesses the bacteriostatic effect of ledaborbactam against ceftibuten-resistant Enterobacterales and determines the required exposure.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Adriana Belas, Catia Marques, Juliana Menezes, Luis Telo da Gama, Patricia Cavaco-Silva, Constanca Pomba
Summary: This study aimed to compare ESBL/pAmpC-producing E. coli strains causing community-acquired UTI in companion animals and humans. The study found that companion animals and humans shared two multidrug-resistant high-risk clonal lineages: ST131 and ST648. The study also reported two newly discovered clonal lineages in humans with community-acquired UTI in Portugal.
Article
Infectious Diseases
Juan Li, Yuqin Song, Jianping Deng, Zhaoran Wang, Nai-Kei Wong, Chao Wang, Gang Zhang, Yang Wang, Shan Lu, Jie Che, Xiaofei Zhao, Zhengdong Zhang, Hong Wang, Ling Zhang, Yunfei Zhang, Xuemei Bai, Min Yuan, Xia Chen, Wen Zhang, Yanwen Xiong, Biao Kan, Jie Feng
Summary: This study explores the dynamics of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) transmission between food-producing animals and humans. The findings suggest that transmission primarily occurs through the high exposure group, which plays a crucial role as a medium, and the carriage rates of shared ARGs are higher in this group compared to the low exposure group.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Michaela Tutone, Truls E. Bjerklund Johansen, Tommaso Cai, Shazad Mushtaq, David M. Livermore
Summary: Urinary tract infections (UTIs) are common worldwide, especially among women. A study investigated the susceptibility of current urinary isolates to fosfomycin and other antibiotics, and found that Escherichia coli was the most common uropathogen isolated and highly susceptible to fosfomycin, nitrofurantoin, and mecillinam.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Biotechnology & Applied Microbiology
Mohammad Taha Ebrahimi, Manouchehr Ahmadi Hedayati, Rima Fanaei Pirlar, Newsha Mortazavi, Mohsen Nazari, Amjad Ahmadi, Jaber Hemmati, Yousef Erfani
Summary: This study investigates the biofilm formation ability and the presence of fimH, afa, and kpsMSTII genes in clinical isolates of ExPEC ST131. The results show that these genes are prevalent in ST131 strains and there are significant differences in biofilm formation ability between clinical E. coli ST131 and non-ST131 isolates. Therefore, suppressing fimH, afa, and kpsMSTII genes may be crucial in treating biofilm infections caused by drug-resistant ST131 strains.
JOURNAL OF APPLIED GENETICS
(2023)
Article
Biology
Qingqing Fang, Yu Feng, Alan McNally, Zhiyong Zong
Summary: Fang et al. identified two previously unidentified phages that can inhibit the growth and decrease the virulence of carbapenem-resistant Klebsiella pneumoniae (CRKP). They also found that CRKP can develop phage resistance but can still be eliminated in a mouse intestinal colonization model, highlighting the potential of phage therapy as a treatment against drug-resistant pathogens.
COMMUNICATIONS BIOLOGY
(2022)
Article
Microbiology
Younjung Kim, Maura Carrai, Marcus H. Y. Leung, Jaime Chin, Jun Li, Patrick K. H. Lee, Julia A. Beatty, Dirk U. Pfeiffer, Vanessa R. Barrs
Summary: The study found that the feline bladder microbiome is typically sparse, with various phyla, families, and genera identified. Different urotypes were associated with disease status, with the Escherichia-Shigella-predominant urotype being common in CKD cases. The microbial composition of CKD cases was more similar to that of E. coli PUC cases than to controls, indicating potential implications for the development of clinical infections.
Article
Microbiology
Sandra J. Aedo, Juechun Tang, Mark P. Brynildsen
Summary: The study showed that stationary-phase Escherichia coli are not susceptible to nitrofurantoin (NIT), but supplementation with glucose under conditions where essential nutrients were absent can increase susceptibility. This insensitivity is mainly due to a low abundance of reducing equivalents, while metabolites can resensitize the bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Jacob Hogins, Ethan Fan, Zheyar Seyan, Sam Kusin, Alana L. Christie, Philippe E. Zimmern, Larry Reitzer
Summary: Urinary tract infections (UTIs) are common in women, especially postmenopausal women, and can be difficult to treat. Escherichia coli is the main cause, but its virulence mechanisms and host-pathogen interactions are complex and varied. Bacterial growth in urine shows significant individual variations.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Qingyuan Yi, Jiaxin Liu, Yufeng Zhang, Hanzhen Qiao, Fang Chen, Shihai Zhang, Wutai Guan
Summary: This study demonstrated that dietary supplementation of anethole can improve the growth performance of weaned piglets challenged with enterotoxigenic Escherichia coli K88 by attenuating intestinal barrier disruption and inflammation.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Haixiu Wang, Eric Cox, Bert Devriendt
Summary: Enterotoxigenic Escherichia coli (ETEC) strains recognize and respond to factors secreted by gut epithelial cells, leading to transcriptional modulation of key ETEC virulence genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Flavia Ivana Mansilla, Cecilia Aristimuno Ficoseco, Maria Hortencia Miranda, Edoardo Puglisi, Maria Elena Fatima Nader-Macias, Graciela Margarita Vignolo, Cecilia Alejandra Fontana
Summary: This study investigated the impact of lactic acid bacteria probiotics on shaping the fecal bacterial community structure of feedlot cattle, showing that probiotics were able to modulate the fecal microbiota and promote health and growth benefits in the animals. The research identified specific bacterial families associated with these benefits and highlighted the potential of probiotics as antibiotic alternatives for commercial feedlot operations.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
J. L. Halleran, B. J. Callahan, M. E. Jacob, H. J. Sylvester, T. Prange, M. G. Papich, D. M. Foster
Summary: The study found that danofloxacin has high intestinal penetration in beef cattle, with significant differences in MIC values for certain bacteria at selected time points between high and low dosing groups. Treatment led to decrease in certain bacterial phyla over time, with a higher number of isolates with clinically relevant resistance found in the low dose group.
SCIENTIFIC REPORTS
(2021)
Article
Ecology
Sam Abraham, Roy N. Kirkwood, Tanya Laird, Sugiyono Saputra, Tahlia Mitchell, Mohinder Singh, Benjamin Linn, Rebecca J. Abraham, Stanley Pang, David M. Gordon, Darren J. Trott, Mark O'Dea
Article
Immunology
Guillaume Le Baut, Claire O'Brien, Paul Pavli, Maryline Roy, Philippe Seksik, Xavier Treton, Stephane Nancey, Nicolas Barnich, Madeleine Bezault, Claire Auzolle, Dominique Cazals-Hatem, Jerome Viala, Matthieu Allez, Jean-Pierre Hugot, Anne Dumay
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Article
Environmental Sciences
Buddhie S. Nanayakkara, Claire L. O'Brien, David M. Gordon
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2019)
Review
Pharmacology & Pharmacy
Muhammad Shamoon, Natalia M. Martin, Claire L. O'Brien
PHARMACOLOGICAL RESEARCH
(2019)
Article
Environmental Sciences
Buddhie S. Nanayakkara, Claire L. O'Brien, David M. Gordon
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2019)
Article
Multidisciplinary Sciences
Carla Camprubi-Font, Paula Bustamante, Roberto M. Vidal, Claire L. O'Brien, Nicolas Barnich, Margarita Martinez-Medina
SCIENTIFIC REPORTS
(2020)
Article
Genetics & Heredity
Marie Touchon, Amandine Perrin, Jorge Andre Moura de Sousa, Belinda Vangchhia, Samantha Burn, Claire L. O'Brien, Erick Denamur, David Gordon, Eduardo Pc Rocha
Review
Nutrition & Dietetics
Georgina M. Williams, Linda C. Tapsell, Claire L. O'Brien, Susan M. Tosh, Eden M. Barrett, Eleanor J. Beck
Summary: Cereal fiber intake can influence gut microbiome composition, diversity, short-chain fatty acid production, and risk factors for metabolic syndrome, but the effects are varied. Further research with better study quality and dietary data is needed to establish a clearer link between cereal fiber intake, microbiome modulation, and metabolic outcomes.
Article
Medicine, General & Internal
Astrid-Jane Williams, Ramesh Paramsothy, Nan Wu, Simon Ghaly, Steven Leach, Sudarshan Paramsothy, Crispin Corte, Claire O'Brien, Catherine Burke, Gabrielle Wark, Dorit Samocha-Bonet, Kelly Lambert, Golo Ahlenstiel, Valerie Wasinger, Shoma Dutt, Paul Pavli, Michael Grimm, Daniel Lemberg, Susan Connor, Rupert Leong, Georgina Hold
Summary: Crohn's disease and ulcerative colitis are common chronic inflammatory bowel diseases with unclear mechanisms. The Australian IBD Microbiota (AIM) Study aims to identify microbial signals and correlations to disease activity over time. By collecting extensive demographic, clinical, lifestyle and dietary data, the study seeks to understand the interaction between IBD patients and their microbiota.
Article
Obstetrics & Gynecology
Jim Parker, Claire O'Brien, Felice L. Gersh
Summary: PCOS is likely inherited as a result of developmental programming of normal gene variants, which can be amplified by in-utero androgen exposure and activated by postnatal lifestyle factors. Endocrine disrupting chemicals may influence the developmental programming of PCOS susceptibility genes.
AUSTRALIAN & NEW ZEALAND JOURNAL OF OBSTETRICS & GYNAECOLOGY
(2021)
Article
Environmental Sciences
Mahboobeh Behruznia, Claire L. O'Brien, David M. Gordon
Summary: The study showed that temperature can explain some of the variation in E. coli isolates recovered per septic tank, while pH is an important factor affecting E. coli diversity. Household size has a significant impact on E. coli population structure, and intraspecific competition may affect the distribution of strains.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Gastroenterology & Hepatology
Rasel Barua, Paul Pavli, David M. Gordon, Claire L. O'Brien
Summary: This study investigates the genomic and phenotypic differences of Escherichia coli strains isolated from different locations in the human gut. While there is no significant evidence suggesting the site-specificity of strains at the genomic level, some phenotypic studies suggest possible site-specificity in the lower gut.
Review
Obstetrics & Gynecology
Jim Parker, Claire O'Brien, Jason Hawrelak
Summary: Diet-induced gastrointestinal dysbiosis may play a significant role in stimulating increased gastrointestinal permeability and systemic inflammation in women with PCOS. Studies have found dysbiosis in most women with PCOS, and preliminary research suggests the involvement of certain substances in the pathophysiological mechanisms of increased intestinal permeability. Treatment with prebiotics, probiotics, and synbiotics appears to have beneficial effects on metabolic and biochemical profiles in PCOS.
OBSTETRICS & GYNECOLOGY SCIENCE
(2022)
Article
Environmental Sciences
Jim Parker, Claire O'Brien, Jason Hawrelak, Felice L. Gersh
Summary: PCOS is increasingly recognized as a complex metabolic disorder in genetically susceptible women following negative exposures to nutritional and environmental factors related to contemporary lifestyle. The mismatch between ancient genetic survival mechanisms and modern lifestyle practices may be responsible for the diverse phenotypes associated with PCOS.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Gastroenterology & Hepatology
Christopher John Kiely, Paul Pavli, Claire Louise O'Brien