Article
Food Science & Technology
Christian Pinna, Angelo Sala
Summary: The study found that hesperidin and diosmin have regulatory effects on human umbilical vein vascular tone, with hesperidin showing stronger vasorelaxation. The vasorelaxant response to hesperidin is influenced by endothelium-dependent reactions, while the vasorelaxation effect of diosmin is associated with NO synthase.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Cardiac & Cardiovascular Systems
Hosam Alden Baksamawi, Alessio Alexiadis, Daniele Vigolo, Alexander Brill
Summary: This article introduces a novel microfluidics vein-on-a-chip model with moving valve leaflets to simulate the hydrodynamics in a vein, combined with a Human Umbilical Vein Endothelial Cell (HUVEC) monolayer. The experiments used a pulsatile flow pattern typical for veins. It was found that unstimulated human platelets accumulated at the luminal side of the leaflet tips proportionally to the leaflet flexibility. Platelet accumulation was induced by thrombin activation, and surprisingly, blockade of glycoprotein (GP) IIb-IIIa slightly increased platelet accumulation. However, blocking the interaction between platelet GPIba and the A1 domain of von Willebrand factor completely abolished platelet deposition. Stimulation of the endothelium with histamine promoted platelet accrual at the basal side of the leaflets.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Nutrition & Dietetics
Lujain A. Almousa, Andrew M. Salter, Marcos Castellanos, Sean T. May, Simon C. Langley-Evans
Summary: Vascular endothelial cells are sensitive to changes in magnesium concentration, which can affect key cellular processes such as cellular organization, apoptosis, protein binding, signal transduction, and metabolism. The study also suggests that magnesium-induced changes may indirectly impact endothelial cell function through other ions.
Article
Hematology
Christopher J. Ng, Alice Liu, Sujatha Venkataraman, Katrina J. Ashworth, Christopher D. Baker, Rebecca O'Rourke, Rajeev Vibhakar, Kenneth L. Jones, Jorge Di Paola
Summary: Using single-cell RNA sequencing and siRNA experiments, we identified altered VWF packaging and release in ECFCs from individuals with low VWF levels, and identified multiple potential regulators of VWF.
Article
Immunology
Archana Joon, Shipra Chandel, Sujata Ghosh
Summary: This study found that EAEC infection can induce an inflammatory response in intestinal epithelial cells through the activation of EGFR pathway and the mediation of downstream effectors.
MICROBES AND INFECTION
(2023)
Article
Hematology
Christine Hough, Colleen Notley, Aomei Mo, Barbara Videl, David Lillicrap
Summary: Substantial phenotypic heterogeneity exists in endothelial cells, and there is a reciprocal relationship between levels of FVIII and VWF. In some endothelial cells, high levels of FVIII co-localize with VWF and are released together.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Cell Biology
Iva Marinovic, Maria Bartosova, Rebecca Herzog, Juan Manuel Sacnun, Conghui Zhang, Robin Hoogenboom, Markus Unterwurzacher, Thilo Hackert, Aurelio A. Teleman, Klaus Kratochwill, Claus Peter Schmitt
Summary: In vitro studies are crucial in pre-clinical research. However, there is limited knowledge on specific characteristics of commonly used mesothelial and endothelial cell lines. In this study, RNA sequencing was performed to comprehensively profile these cell lines and identify distinct gene expression patterns and biological processes. The findings provide important information for the appropriate use of these cell lines in research.
Article
Biochemistry & Molecular Biology
Zhihao Wang, Biao Li, Haiyan Jiang, Yuchen Ma, Yanni Bao, Xiangyu Zhu, Hongguang Xia, Yong Jin
Summary: Alcoholic fatty liver disease (AFLD) is liver damage caused by chronic heavy drinking, with IL-8 potentially playing a role in its pathogenesis. Studies have shown that hIL-8 exacerbates alcohol-induced fatty liver disease in mice through the Akt/HIF-1 alpha pathway, highlighting a potential target for clinical treatment.
Article
Cell Biology
Archana Joon, Shipra Chandel, Sujata Ghosh
Summary: This study evaluated the role of Toll-like receptors (TLRs) in epidermal growth factor receptor (EGFR)-mediated IL-8 secretion by EAEC-infected human small intestinal and colonic epithelial cells. It was found that EAEC infection could upregulate the transcripts of TLR2, TLR4 and TLR5 in these cells, with the highest level observed in cells infected with EAEC-T8. These TLRs contributed significantly to EGFR activation and were associated with downstream effectors and transcription factors of the EGFR-mediated signal transduction pathways. Additionally, these TLRs were also involved in IL-8 secretion by both types of infected cells.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biotechnology & Applied Microbiology
Yuxiang Ge, Yixuan Li, Zixu Wang, Lan Li, Huajian Teng, Qing Jiang
Summary: The study found that early application of dynamic compression had a suppressive effect on the expression of chondrocyte-specific markers, while late application of dynamic compression promoted cartilage matrix gene expression and suppressed hypertrophic development. Dynamic mechanical compression loading not only promotes chondrogenic differentiation of SMSCs, but also plays a vital role in maintaining the cartilage phenotype.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuchen Ma, Yanni Bao, Lifang Wu, Yijun Ke, Lina Tan, Huan Ren, Jue Song, Qian Zhang, Yong Jin
Summary: Liver fibrosis is characterized by excessive accumulation of extracellular matrix (ECM) due to chronic liver injury. Hepatic stellate cells (HSCs) and interleukin-8 (IL-8) play significant roles in the development and progression of liver fibrosis through ECM synthesis and inflammation promotion.
MOLECULAR IMMUNOLOGY
(2022)
Article
Dermatology
Shunsuke Miura, Sandra Garcet, Xuan Li, Inna Cueto, Charissa Salud-Gnilo, Norma Kunjravia, Kazuhiko Yamamura, Juana Gonzalez, Mika Murai-Yamamura, Darshna Rambhia, James G. Krueger
Summary: LL37, produced by skin injury and bacterial infection, is involved in the early stages of psoriasis. The intracellular receptors TLR3, TLR7, TLR8, and TLR9 are thought to play a role in the pathogenesis of psoriasis in conjunction with LL37. However, the interaction between TLR7/8 and LL37 in keratinocytes remains unclear. This study aimed to clarify this relationship and the involvement of TLR7/8 and LL37 in psoriasis.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Immunology
Burcak Yesildag, Joan Mir-Coll, Aparna Neelakandhan, Claire B. Gibson, Nikole R. Perdue, Chantal Rufer, Maria Karsai, Adelinn Biernath, Felix Forschler, Patricia Wu Jin, Patrick M. Misun, Alexandra Title, Andreas Hierlemann, Frederik F. Kreiner, Johnna D. Wesley, Matthias G. von Herrath
Summary: In this study, three in-vitro islet-immune injury models were established to investigate the immunopathogenesis of type 1 diabetes. The results showed that liraglutide protected pancreatic cells from immune attack.
CLINICAL IMMUNOLOGY
(2022)
Article
Surgery
Guillermo Monux, Mariano de la Serna-Soto, Ferran Pla-Sanchez, Jose J. Zamorano-Leon, Antonio Segura, Rodrigo Rial, Gala Freixer, Khaoula Zekri-Nechar, Carlos Hugo-Martinez, Javier Serrano, Antonio Lopez-Farre
Summary: Compression stocking therapy can increase the levels of biomarkers associated with endothelial functionality, inflammation, oxidative stress, and coagulation in the varicose vein wall.
JOURNAL OF VASCULAR SURGERY-VENOUS AND LYMPHATIC DISORDERS
(2021)
Article
Multidisciplinary Sciences
Arezoo Beig Parikhani, Kowsar Bagherzadeh, Rada Dehghan, Alireza Biglari, Mohammad Ali Shokrgozar, Farhad Riazi Rad, Sirous Zeinali, Yeganeh Talebkhan, Soheila Ajdary, Reza Ahangari Cohan, Mahdi Behdani
Summary: A rational computational approach was used to develop novel triple-mutant IL-2 variants for improving IL-2-based immunotherapy. The mutants showed lower affinity to IL-2Ra compared to wild-type IL-2, but did not significantly disturb the interaction of IL-2 with IL-2Rβγc subunits.