Journal
CHEMICAL SCIENCE
Volume 6, Issue 2, Pages 1324-1333Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4sc03200d
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Funding
- NSF [CHE-1402753]
- Welch Foundation [F-1155]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1402753] Funding Source: National Science Foundation
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Ultraviolet photodissociation (UVPD) mass spectrometry is employed to investigate the structure of holo-myoglobin as well as its apo form transferred to the gas phase by native electrospray. UVPD provided insight into the stability of native structural elements of holo-myoglobin. The fragmentation yields from UVPD showed the greatest overall correlation with B-factors generated from the crystal structure of apomyoglobin, particularly for the more disordered loop regions. Solvent accessibility measurements also showed some correlation with the UVPD fragmentation of holo-myoglobin. Comparison of UVPD of holo-and apo-myoglobin revealed similarities in fragmentation yields, particularly for the lower charge states (8 and 9+). Both holo- and apo-myoglobin exhibited low fragmentation yields for the AGH helical core, whereas regions known to interact with the heme show suppressed fragmentation for holo-myoglobin. The fragment yields from HCD showed the lowest correlation with B-factor values and rather reflected preferential charge-directed backbone cleavages.
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