Article
Biochemistry & Molecular Biology
Vendula Horackova, Lubos Voleman, Kari D. Hagen, Marketa Petru, Martina Vinopalova, Filip Weisz, Natalia Janowicz, Lenka Markova, Alzbeta Motyckova, Vladimira Najdrova, Pavla Tumova, Scott C. Dawson, Pavel Dolezal
Summary: This study demonstrated successful genome editing of G. intestinalis using CRISPR/Cas9 technology, achieving effective gene knockout and providing important tools for further research on parasitic protists.
Article
Public, Environmental & Occupational Health
Tanja E. Peters, Benno Kreuels, Marylyn M. Addo, Egbert Tannich, Camilla Rothe
Summary: This study analyzed 339 cases of returning travellers with confirmed giardiasis between 2007 and 2016 in Hamburg, Germany, finding a significantly higher risk of treatment failure with metronidazole for patients returning from South Asia. Second-line treatment regimens showed inconsistency and unsatisfactory results, suggesting a need for reevaluation of treatment strategies for giardiasis. Prospective trials are urgently needed to assess new first-line treatment options and improve second-line treatment strategies.
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2021)
Article
Virology
Leticia F. Baumbach, Ana Cristina S. Mosena, Raquel S. Alves, Laura J. Camargo, Juliana C. Olegario, Leonardo R. Lobraico, Joao Marcos N. Costa, Mauro R. Borba, Fernando V. Bauermann, Matheus N. Weber, Claudio W. Canal
Summary: Pestiviruses, which cause economic losses to the cattle industry, have been well described in some regions of Brazil. However, there is a lack of information about pestiviruses in the Amazon Region. This study investigated the prevalence and genetic variability of ruminant pestiviruses in northern Brazil and found a high prevalence of all bovine pestiviruses, including HoBiPeV.
Article
Virology
Luiz V. L. Moreira, Andreza P. Malheiros, Kemere M. Barbosa, Pedro E. B. Freitas, Andrea L. Silva, Ana C. R. Cruz, Heloisa M. Nunes, Andre A. C. das Chagas, Alex J. S. de Souza
Summary: This study retrospectively evaluated the serological and molecular prevalence of HAV infection in patients with acute hepatitis in the Brazilian Eastern Amazon. The results showed that males and children under 10 years old are at a higher risk of HAV infection. Genotyping revealed that HAV subgenotype IA was the most common.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Microbiology
Malgorzata Smogula, Roland Wesolowski, Marta Pawlowska, Celestyna Mila-Kierzenkowska
Summary: Giardia intestinalis is a common food-borne protozoan, and 22 selected factors were found to have an impact on its survival assessment and detection. Microscopic method showed significant influence compared to real-time PCR. Prevention of infection is crucial, and further research and epidemiological observation are needed.
Article
Infectious Diseases
Josileide Araujo da Silva, Alessandra Scofield, Flavia de Nazare Barros, Diana Maria de Farias, Gabriela Riet-Correa, Pedro Soares Bezerra Junior, Tiago Felipe Souza Santos, Gabriel Savio Fernandes Tavares, Leonardo Carreira Trevelin, Giselle Souza da Paz, Valiria Duarte Cerqueira
Summary: This study investigated natural H. capsulatum infection in bats from forested and urban areas in the state of Para, Brazil. Two out of 100 bats analysed were confirmed to be positive for H. capsulatum. DNA sequencing revealed a 100% match with H. capsulatum DNA, indicating the presence of the fungus in the bat populations of this region.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Immunology
Kristyna Brozova, Milan Jirku, Zuzana Lhotska, Dana Kvetonova, Oldriska Kadlecova, Christen Rune Stensvold, Peter Samas, Klara J. Petrzelkova, Katerina Jirku
Summary: This study investigated the presence and epidemiology of Giardia in healthy volunteers in the Czech Republic and compared different diagnostic methods. The results showed that qPCR is the most sensitive method for detecting Giardia, and the prevalence of infection in asymptomatic individuals was 7%. Contact with animals was found to be the only factor significantly impacting the incidence of Giardia in healthy humans.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Medicine, General & Internal
Narjara Campos de Araujo, Orivaldo Florencio de Souza, Mauro Jose de Deus Morais, Francisco Naildo Cardoso Leitao, Italla Maria Pinheiro Bezerra, Luiz Carlos de Abreu, Luciano Miller Reis Rodrigues
Summary: Musculoskeletal disorders are gradually affecting workers worldwide, compromising their occupational health and quality of life. A study in Brazil found that motorcycle taxi drivers in Rio Branco, Acre, have a high prevalence of musculoskeletal symptoms, particularly in the lumbar region, impacting their sleep and causing headaches.
Article
Immunology
Zhiqiang Li, Dimitra Peirasmaki, Staffan Svard, Magnus Abrink
Summary: The proteoglycan serglycin plays a regulatory role in intestinal immune responses by influencing the expression levels of chemokines and cytokines, leading to delayed weight gain in young SG(-/-) mice infected with Giardia intestinalis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Pavla Tumova, Lubos Voleman, Andreas Klingl, Eva Nohynkova, Gerhard Wanner, Pavel Dolezal
Summary: The presence of mitochondria is a distinguishing feature between prokaryotic and eukaryotic cells. Eukaryotes control mitochondrial inheritance mainly due to the presence of the genetic component, but little is known about the segregation of mitochondria to daughter cells during cell division. Anaerobic eukaryotic microbes evolved genomeless mitochondria-related organelles (MROs) providing a distinct mechanistic basis for maintenance of stable mitochondrial numbers.
Article
Immunology
Olivier Duron, Rachid Koual, Lise Musset, Marie Buysse, Yann Lambert, Benoit Jaulhac, Denis Blanchet, Kinan Drak Alsibai, Yassamine Lazrek, Loic Epelboin, Pierre Deshuillers, Celine Michaud, Maylis Douine
Summary: A case of unusual human anaplasmosis in the Amazon rainforest of French Guiana is reported. Molecular typing revealed that the pathogen is a novel Anaplasma species, distinct from all known species, and more genetically related to recently discovered Anaplasma spp. causing infections in rainforest wild fauna of Brazil.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Medicine, General & Internal
Aleksandra Lanocha, Natalia Lanocha-Arendarczyk, Dominika Wilczynska, Barbara Zdziarska, Danuta Kosik-Bogacka
Summary: This study aimed to evaluate the frequency of gastrointestinal protozoan infection in patients with hematological malignancies undergoing intensive treatment and to determine the biological factors influencing intestinal parasite infection. The results showed a higher prevalence of parasitic infections in patients with hematological malignancies undergoing treatment.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jana Caudet, Maria Trelis, Susana Cifre, Gabriela Tapia, Jose M. Soriano, Regina Rodrigo, Juan F. Merino-Torres
Summary: The diagnosis of obesity and the development of insulin resistance and metabolic comorbidities are linked. Enteric eukaryotic unicellular parasites may play an important role in modulating the antioxidant defenses of an obese host, thus potentially beneficial in the development of systemic metabolic disorders.
Article
Microbiology
Ricardo Ishak, Marluisa de Oliveira Guimaraes Ishak, Isabella Nogueira Abreu, Luiz Fernando Almeida Machado, Sandra Souza Lima, Maria Alice Freitas Queiroz, Izaura Maria Cayres-Vallinoto, Joao Farias Guerreiro, Antonio Carlos Rosario Vallinoto
Summary: HTLV-2 has been prevalent in Brazilian indigenous populations for over 30 years, mainly transmitted through sexual intercourse and mother-to-child transmission. Five selected publications showed the presence of HTLV-2 in 24 out of 41 communities with a prevalence rate of up to 41.2% among the Kayapo villages. Two high endemicity pockets were identified in Para state, indicating the epicenter of HTLV-2 in the Amazon region of Brazil. The prevalence rates among the Kayapo have been declining over the years, especially among females, suggesting the influence of sociocultural and behavioral factors, as well as targeted public health policies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Xingang Yu, Hongcai Wang, Yilong Li, Xuanru Mu, Kaijian Yuan, Anfeng Wu, Jianchao Guo, Yang Hong, Haoji Zhang
Summary: The occurrence of Blastocystis spp., Enterocytozoon bieneusi, and Giardia duodenalis infections in Leizhou black goats in China was reported. The study found that Blastocystis spp., E. bieneusi, and G. duodenalis infections were common in Leizhou black goats, and they carried zoonotic genotypes, indicating a potential risk of zoonotic transmission.
Article
Geography, Physical
Dario Alejandro Ramirez, Mariana Fabra, Samanta Xavier, Alena Mayo Iniguez
Summary: Experimental paleoparasitological approaches were used to investigate the impact of dehydration and local soil on parasite egg preservation in ancient fecal samples. The study found that desiccation and soil mixing significantly decreased the number of surviving eggs in experimental coprolites. This suggests that the climate and soil conditions in which archaeological fecal samples are found can greatly influence the preservation of parasite evidence from past populations.
Article
Veterinary Sciences
Polyanna A. A. Bacelar, Lauren H. Jaeger, Deiviane A. Calegar, Jessica P. Santos, Beatriz Coronato-Nunes, Elis R. C. Reis, Marcio N. Boia, Kerla J. L. Monteiro, Filipe A. Carvalho-Costa
Summary: This study investigated the species composition and genetic diversity of Metastrongylus infection in pigs in impoverished rural communities in northeastern Brazil. The results showed that M. salmi exhibited low genetic diversity in these pig populations.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2022)
Article
Microbiology
Deiviane A. Calegar, Beatriz C. Nunes, Kerla J. L. Monteiro, Polyanna A. A. Bacelar, Brenda B. C. Evangelista, Mayron M. Almeida, Jurecir Silva, Jessica P. Santos, Marcio N. Boia, Lauren H. Jaeger, Filipe A. Carvalho-Costa
Summary: Subclinical giardiasis is endemic in Brazilian communities in different biogeographic regions, showing high genetic diversity and heterogeneous genotypic distribution.
Article
Public, Environmental & Occupational Health
Polyanna A. A. Bacelar, Kerla J. L. Monteiro, Jessica P. dos Santos, Denilson de A. Silva, Daniella N. N. Leal, Mayron M. M. Almeida, Brenda B. C. Evangelista, Francisco M. de Oliveira-Neto, Filipe A. A. Carvalho-Costa
Summary: This study presents the morphological characterization of Strongylida larvae recovered from humans and pigs living in close proximity, exploring possible zoonotic cycles. The findings reveal the presence of various Strongylida species in human and pig fecal samples, highlighting the importance of integrated approaches in studying intestinal helminthiasis.
JOURNAL OF TROPICAL MEDICINE
(2022)
Article
Entomology
Andres Gomez-Palacio, Sebastian Pita, Fernando Abad-Franch, Yoman Monsalve, Omar Cantillo-Barraza, Fernando A. Monteiro, Marcio G. Pavan, Walter S. Santos, Alejandra Panzera, Gabriela Burgueno-Rodriguez, Francisco Panzera
Summary: Through genetic and cytogenetic studies, interpopulation genetic diversity and phylogenetic affinities of T. maculata were investigated. The findings suggest that T. maculata may consist of a complex of at least three sibling taxa.
MEDICAL AND VETERINARY ENTOMOLOGY
(2023)
Article
Virology
Gabriela A. Garcia, Anton R. Lord, Lilha M. B. Santos, Tharanga N. Kariyawasam, Mariana R. David, Dinair Couto-Lima, Aline Tatila-Ferreira, Marcio G. Pavan, Maggy T. Sikulu-Lord, Rafael Maciel-de-Freitas
Summary: With the increasing transmission of dengue and Zika viruses worldwide, the establishment of an efficient arbovirus surveillance system is crucial for early warning systems and preparedness. Near Infrared Spectroscopy (NIRS) is a promising technique for detecting arbovirus infection in mosquitoes due to its cost and time effectiveness, reagent-free nature, and non-invasive characteristics. The study demonstrates that NIRS can accurately differentiate Ae. aegypti mosquitoes infected with either ZIKV or DENV, and those coinfected with ZIKV/DENV from uninfected ones. The results suggest that NIRS should be further tested in field settings to assess its potential role in monitoring natural infection in field-caught mosquitoes.
Article
Immunology
Gabriel Carvalho de Macedo, Wanessa Teixeira Gomes Barreto, Carina Elisei de Oliveira, Filipe Martins Santos, Grasiela Edith de Oliveira Porfirio, Samanta Cristina das Chagas Xavier, Fernanda Moreira Alves, Alanderson Rodrigues da Silva, Gisele Braziliano de Andrade, Andreza Castro Rucco, William Oliveira de Assis, Ana Maria Jansen, Andre Luiz Rodrigues Roque, Heitor Miraglia Herrera
Summary: The aim of this study was to investigate the occurrence of Leishmania infantum in South American coatis inhabiting two forest fragments in Campo Grande, Brazil. The results showed that 33 out of 110 coatis were infected with L. infantum, and the infection rate was significantly higher in the VBA area. The study suggested that the higher infection rate in VBA may be attributed to the presence of resident dogs and chickens, a denser population of coatis, and physical barriers in the surroundings.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biodiversity Conservation
Cecilia S. Andreazzi, Luis A. Martinez-Vaquero, Gisele R. Winck, Thiago S. Cardoso, Bernardo R. Teixeira, Samanta C. C. Xavier, Rosana Gentile, Ana Maria Jansen, Paulo S. D'Andrea
Summary: Land use changes and biodiversity loss disrupt ecosystem functioning and drive infectious disease outbreaks. Using a large database on small mammal infection by Trypanosoma cruzi in Brazil, we found that allometric traits and rodent abundance were key predictors of infection risk, along with variables related to landscape environmental quality. Changes in natural vegetation cover and biodiversity dimensions indirectly reduced infection by affecting host community composition. Integrating social inclusion and human welfare into biodiversity conservation and restoration efforts could help regulate T. cruzi prevalence and reduce transmission risk.
Article
Virology
Martha Thieme Petersen, Dinair Couto-Lima, Gabriela Azambuja Garcia, Marcio Galvao Pavan, Mariana Rocha David, Rafael Maciel-de-Freitas
Summary: This study investigates the effects of Wolbachia infection and dengue-1 virus DENV-1 on the life-history traits of Ae. aegypti. The results show that Wolbachia infection has no significant impact on mosquito survival and fecundity, but decreases oviposition success. DENV-1 has a slight protective effect on the viability of stored eggs.
Article
Multidisciplinary Sciences
Raphael Testai, Marinez Ferreira de Siqueira, Diogo Souza Bezerra Rocha, Andre Luiz Rodrigues Roque, Ana Maria Jansen, Samanta Cristina das Chagas Xavier
Summary: Ecological Niche Modeling was applied to investigate the niche of Didelphis aurita and the infection of Trypanosoma cruzi in the Brazilian Atlantic Rainforest. The presence of two common vector species, Triatoma vitticeps and Panstrongylus megistus, was used to predict the occurrence of D. aurita. The Ecoland method revealed the varying impact of D. aurita on the enzootic cycle of T. cruzi in different locations.
Article
Virology
Livia Mara Silva, Lorena Rodrigues Riani, Juliana Brovini Leite, Jessica Mara de Assis Chagas, Laura Silva Fernandes, Romario Costa Fochat, Carmen Gomide Pinto Perches, Thiago Cesar Nascimento, Lauren Hubert Jaeger, Marcelo Silva Silverio, Olavo dos Santos Pereira-Junior, Frederico Pittella
Summary: The detection of SARS-CoV-2 variants through RT-qPCR can be affected. The Omicron variant exhibits a higher affinity for the upper respiratory system and causes symptoms similar to bronchitis, unlike other SARS-CoV-2 variants that cause pneumonitis-like conditions. This characteristic increases the viscosity of clinical samples, leading to control gene expression failure. A rapid nucleic acid extraction step was optimized to restore gene expression and detect the presence of the Omicron variant in clinical samples, improving the quality of RT-qPCR results.
Review
Chemistry, Multidisciplinary
Olivia B. O. Moreira, Bruna M. Berlande, Jessica C. Q. de Souza, Joao Marcos B. Candido, Lauren H. Jaeger, Luiz Henrique C. Adriano, Marco Antonio D. A e Brito, Maria Patricia do Nascimento, Patricia A. Geraldo, Paula R. Chellini, Marcone Augusto L. de Oliveira
Summary: Capillary electromigration techniques are used to separate neutral compounds, solvated ions, and ionized species by exploiting their differential migration when subjected to an electrical field in a capillary column. It is a reliable method for quantitatively analyzing a wide range of compounds in bodily fluids. Capillary electrophoresis (CE) is an advantageous strategy for enhancing traditional clinical diagnostic methods and developing new ones, particularly in the field of precision medicine. This review discusses traditional methods for analyzing blood, urine, saliva, feces, and sweat, and highlights how CE can enable faster, automated, and cost-effective analysis, leading to improved diagnostic possibilities for patients.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2023)
Article
Parasitology
Polyanna Araujo Alves Bacelar, Kerla Joeline Lima Monteiro, Deiviane Aparecida Calegar, Jessica Pereira dos Santos, Beatriz Coronato-Nunes, Elis Regina Chaves dos Reis, Marcio Neves Boia, Lauren Hubert Jaeger, Filipe Anibal Carvalho-Costa
Summary: This study aimed to explore the species composition and genetic diversity of Oesophagostomum spp. infecting pigs in close contact with humans in the state of Piaui, Brazil. Through molecular analysis and parasitological tests, it was found that O. dentatum, O. quadrispinulatum, and O. columbianum were the species present in pigs. O. dentatum and O. quadrispinulatum were closely related and formed a monophyletic group with O. aculeatum.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2022)