Article
Chemistry, Analytical
Yan Bai, Yao Wang, Liping Cao, Yongjian Jiang, Yuanfang Li, Hongyan Zou, Lei Zhan, Chengzhi Huang
Summary: A new type of carbon quantum dots with mitochondria-targeting function was prepared in this study, showing excellent fluorescence turn-off response for accurate ONOO- imaging. The linear correlation between quenching efficiency and ONOO- concentration was demonstrated, with successful in situ visualization of endogenous ONOO- generated in live cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qingfeng Xia, Shumin Feng, Jiaxin Hong, Guoqiang Feng
Summary: A novel probe ONOO-LysopH has been developed to detect peroxynitrite and track lysosomal pH changes in live cells. The probe is based on a new rhodamine derivative with near-infrared emission and large Stokes shift, showing quick, sensitive, and selective responses to peroxynitrite and acid, providing a potential multifunctional tool for live systems.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Congcong Zhang, Haixiao Fang, Wei Du, Duoteng Zhang, Yunwei Qu, Fang Tang, Aixiang Ding, Kai Huang, Bo Peng, Lin Li, Wei Huang
Summary: In this study, a derivative of N,N-dimethyl-naphthalenamine was discovered to effectively detect MAO-A with high affinity and selectivity. It was also used for in situ imaging of MAO-A in living cells, enabling a comparison of the MAO-A content in human glioma and paracancerous tissues.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Qingyi Han, Dingding Zhang, Rui Zhang, Jianpu Tang, Keying Xu, Mingzheng Shao, Yuyan Li, Peiyao Du, Ruizhong Zhang, Dayong Yang, Libing Zhang, Xiaoquan Lu
Summary: A DNA-functionalized metal-organic framework (MOF)-based ratiometric fluorescent probe was developed for sensitive detection and imaging of miRNA-21 in living cells. The probe achieved picomolar limit detection without signal amplification and allowed imaging of intracellular miRNA-21 through strand-displacement reaction, enabling discrimination between cancer cells and normal cells. This work presents a new approach for intracellular molecular analysis and has significant implications for early disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Agriculture, Multidisciplinary
Huan Peng, Suna Kong, Xia Deng, Qirong Deng, Fengpei Qi, Changhui Liu, Ruiren Tang
Summary: Determining the concentration of SO2 derivatives is crucial for food safety and health. A ratiometric fluorescent probe was developed in this study, which can accurately detect the concentration of SO2 derivatives by colorimetric and ratiometric changes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Spectroscopy
Tiantian Jiang, Weiwei Bian, Jianfei Kan, Yanyan Sun, Ning Ding, Wenjing Li, Jin Zhou
Summary: A new probe RB-CR for detecting Cr3+ was constructed based on the conjugation between rhodamine B and phenylthiourea, showing selectively, sensitively and rapidly response to Cr3+ complexation. The fluorescence imaging study suggested its potential application as a surveillance probe in living cells with low cytotoxicity, providing feasibility for exploring the Cr3+ recognition process.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Mingtai Sun, Tian Wang, Xin Yang, Huan Yu, Suhua Wang, Dejian Huang
Summary: A new fluorescent probe BV-1 based on BODIPY group was synthesized and demonstrated to detect viscosity in living cells with high selectivity and sensitivity. The probe showed significant enhancement in fluorescence with redshift in emission wavelength under high viscosity conditions, providing efficient ways for the development of viscosity probes and monitoring of intracellular viscosity properties and functions.
Article
Chemistry, Applied
Hui Sun, Qingyu Xu, Chen Xu, Yukun Zhang, Jindong Ai, Mingguang Ren, Keyin Liu, Fangong Kong
Summary: In this study, a rhodamine polymer fluorescent probe (PEG-R) was designed and synthesized, with improved water solubility by the introduction of polyethylene glycol, enhancing its performance and broadening its application in the food field. The PEG-R exhibited high sensitivity, selectivity, and fast response to Cu2+, with a approximately 29-fold fluorescence enhancement and a limit of detection (LOD) of 1.295 x 10-6 M. This probe can be used for determining Cu2+ in living cells, zebrafish, white wine, and food products, and can be made into practical gels and test strips.
Article
Chemistry, Applied
Nansong Zhu, Xiaolei Guo, Yulei Chang, Zhan Shi, Long Yi Jin, Shouhua Feng
Summary: This study reports a fluorescent probe, RIC, that can detect mitochondrial dynamics and hypochlorous acid (HOCl) in real time with high selectivity and sensitivity. RIC is anchored in mitochondria and can reveal changes in mitochondrial dynamics while detecting HOCl in seconds. It has a low detection limit, is pH-insensitive, and can analyze both endogenous and exogenous HOCl. Furthermore, the study proves that the molecular design strategy provides a general synthetic method for constructing new multifunctional probes for HOCl.
Article
Chemistry, Analytical
Liu Yang, Guopan Liu, Qingxin Chen, Yingpeng Wan, Zhiyang Liu, Jie Zhang, Chen Huang, Zhiqiang Xu, Shengliang Li, Chun-Sing Lee, Liang Zhang, Hongyan Sun
Summary: Cellular senescence plays a significant role in various physiological processes, and the accumulation of senescent cells can lead to age-related diseases. In this study, a beta-gal-activated probe, MB-beta gal, was designed to detect and eliminate senescent cells, demonstrating fast response, high sensitivity, and high selectivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Jingcan Qin, Fei Kong, Yuanyuan Guo, Dongya Wang, Chuan Zhang, Yuehua Li
Summary: In this study, a novel ratiometric fluorescent probe based on a D-pi-A-A structure was developed. The probe displayed good selectivity and high sensitivity towards bisulfite in aqueous solution. It exhibited excellent two-photon fluorescence properties and was successfully used for fluorescence imaging of bisulfite in MCF-7 cells and rat liver tissues. Furthermore, the probe showed potential for practical application in measuring bisulfite content in real food samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yibin Zhang, Dilka Liyana Arachchige, Adenike Olowolagba, Rudy L. Luck, Haiying Liu
Summary: A near-infrared fluorescent probe was developed for specific detection of NADH in live cells. The probe utilizes a closed spironolactone switch to turn off fluorescence, but fluorescence is turned on when the probe is reduced by NADH.
Review
Chemistry, Inorganic & Nuclear
A. Afrin, P. Chinna Ayya Swamy
Summary: The development of fluorescent turn-on receptors for selective and sensitive detection of specific ions is an active research area. The recognition and monitoring of biologically important metal ions via fluorescence turn-on mechanism is important. Schiff base-based chemosensors have garnered attention due to their optical properties, thermal stability, and ease of synthesis. There are several fluorescent organic molecules that can be incorporated into Schiff bases to create effective chemosensors for specific metal ions. However, developing functionalized fluorescence probes with biological activity and multi-analyte response is challenging. This review provides an overview of fluorescent Schiff base probes for selective and sensitive detection of one-to-multi metal ions and their applications in cellular imaging, emphasizing the importance of monitoring multiple metal ions simultaneously.
COORDINATION CHEMISTRY REVIEWS
(2023)
Review
Chemistry, Multidisciplinary
Xiao Liu, Young-Tae Chang
Summary: This article outlines the concepts and mechanisms of different strategies for live cell discrimination and introduces their applications in cell-type discrimination. These strategies include protein-oriented, carbohydrate-oriented, DNA-oriented, gating-oriented, metabolism-oriented, and lipid-oriented approaches. Fluorescent probes can achieve live cell distinction by interacting with biomolecules, accumulating via transporters, and participating in metabolism.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Physics, Applied
Yonggang Yang, Yang Liu, Beidou Feng, Chaochao Qin, Tiantian Guan, Ge Wang, Hua Zhang, Yufang Liu
Summary: Transient absorption (TA) signals, with real-time multi-signals and high sensitivity, are less disturbed by the biological environment compared to single fluorescence signals. Intelligently regulated probes (1-VBM and 1-VBH) were designed and synthesized to utilize time-resolved excited-state absorption (ESA) and stimulated emission (SE) signals in TA spectroscopy. The ESA signals in TA spectroscopy are reliable markers for detecting viscosity in biological samples and can be a potential tool for clinical diagnosis.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Applied
Giulia De Soricellis, Francesco Fagnani, Alessia Colombo, Claudia Dragonetti, Dominique Roberto, Daniele Marinotto, David H. Hartnell, Mark J. Hackett, Massimiliano Massi, Bertrand Carboni, Veronique Guerchais
Summary: Three novel luminescent iridium(III) dyes with tryptophan amino acid and cyclometalated 2-phenylpyridines, 2-(2,4-difluorophenyl)pyridines or 2-phenylquinolines have been synthesized. They exhibit emissions at 522-561 nm with luminescence quantum yields ranging from 0.33 to 0.98. These dyes can effectively stain neuronal cells in rat cerebellum tissue, showing strong affinity for granule neurons. The complexes with cyclometalated 2-(2,4-difluorophenyl)pyridines or 2-phenylquinolines also exhibit good affinity for brain white matter. Among them, the dye with two cyclometalated 2-phenylquinolines has the highest luminescence quantum yield (0.98) and demonstrates the strongest affinity for purkinje neurons in cerebellum tissue.
Article
Chemistry, Organic
Daniel Pecorari, Emanuele Giuliani, Andrea Mazzanti, Stefano Stagni, Valentina Fiorini, Giulia Vigarani, Francesco Zinna, Gennaro Pescitelli, Michele Mancinelli
Summary: Bis-aryl carbazole borane derivatives with emissive properties and axial chirality were synthesized. The resolution of a thermally stable atropisomeric pair (compound 1b), which has a B-C chiral axis, was achieved using chiral stationary-phase high-performance liquid chromatography (CSP-HPLC). The complete photophysical properties of all compounds were measured and simulated using time-dependent density functional theory (TD-DFT) calculations, and circularly polarized luminescence spectra were obtained for the atropisomers of compound 1b, with their absolute configuration determined by a TD-DFT simulation of the electronic circular dichroism (ECD) spectra.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Review
Nanoscience & Nanotechnology
Peije Russell, Lars Esser, Christoph E. E. Hagemeyer, Nicolas H. H. Voelcker
Summary: COVID-19-induced thromboinflammation can lead to severe complications such as pulmonary embolisms, ischaemic stroke, and myocardial infarctions. The long-term risk of cardiovascular diseases also significantly increases in COVID-19 survivors. Nanomedicine shows promising potential in protecting pharmaceuticals and delivering targeted treatments. Developing nanomedicine for COVID-19-induced thromboinflammation, including dual-therapeutic approaches, may greatly aid in combating the ongoing pandemic.
NATURE NANOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ashley J. Mulder, Rhys D. Tilbury, Melissa V. Werrett, Phillip J. Wright, Payal Patel, Thomas Becker, Franca Jones, Stefano Stagni, Guohua Jia, Massimiliano Massi, Mark A. Buntine
Summary: This study reports on the effects of different N-donor ligands on the chemical composition and surface plasmon resonance (SPR) transition of copper nanoparticles (CuNPs) formed via laser ablation. Pyridines and alkylated tetrazoles showed minimal effects on the SPR transition, while the presence of tetrazoles resulted in a significant blue shift. This shift was attributed to the tetrazolate anions providing a reducing environment, preventing the formation of copper(II) oxides.
Article
Oncology
Jessica M. Logan, Ashley M. Hopkins, Carmela Martini, Alexandra Sorvina, Prerna Tewari, Sarita Prabhakaran, Chelsea Huzzell, Ian R. D. Johnson, Shane M. Hickey, Ben S. -Y. Ung, Joanna Lazniewska, Robert D. Brooks, Courtney R. Moore, Maria C. Caruso, Litsa Karageorgos, Cara M. Martin, Sharon O'Toole, Laura Bogue Edgerton, Mark P. Ward, Mark Bates, Stavros Selemidis, Adrian Esterman, Sheena Heffernan, Helen Keegan, Sarah Ni Mhaolcatha, Roisin O'Connor, Victoria Malone, Marguerite Carter, Katie Ryan, Andres Clarke, Nathan Brady, Sonja Klebe, Hemamali Samaratunga, Brett Delahunt, Michael J. Sorich, Kim Moretti, Lisa M. Butler, John J. O'Leary, Douglas A. Brooks
Summary: A novel biomarker panel (Appl1, Sortilin and Syndecan-1) was found to be a strong independent predictor for both clinical and biochemical recurrence outcomes, with higher prediction performance than traditional grading. The study also found that biomarker-assisted assessment had better predictive performance for evaluating the risk of biochemical recurrence and clinical recurrence in patients with prostate cancer.
Article
Pharmacology & Pharmacy
Ishdeep Kaur, Terence Tieu, Veerasikku G. Deepagan, Muhammad A. Ali, Fahad Alsunaydih, David Rudd, Maliheh A. Moghaddam, Laure Bourgeois, Timothy E. Adams, Kristofer J. Thurecht, Mehmet Yuce, Anna Cifuentes-Rius, Nicolas H. Voelcker
Summary: Despite the clinical benefits of chemotherapeutics, drug resistance remains a major challenge in breast cancer treatment. Nanomedicines, such as porous silicon nanoparticles (pSiNPs), offer a targeted and effective approach by delivering multiple therapeutics and minimizing drug resistance. In this study, breast cancer-targeted pSiNPs co-loaded with an anticancer drug and gold nanoclusters (AuNCs) were engineered. The combination of hyperthermia and chemotherapy using targeted pSiNPs showed higher cell-killing efficacy compared to monotherapy and a nontargeted system with combined therapeutics. These findings highlight the potential of targeted pSiNPs as a versatile platform for personalized medicine.
Article
Materials Science, Multidisciplinary
Muamer Dervisevic, Nicolas H. Voelcker
Summary: Transdermal electrochemical sensors based on microneedle array with integrated conductive microcavities were developed to protect the sensing layer during insertion and removal from the skin. The platforms retain their functionality and sensitivity even after multiple skin applications. This approach has great potential in advancing transdermal sensing technology and providing reliable and cost-effective sensing devices.
ACS MATERIALS LETTERS
(2023)
Article
Chemistry, Analytical
Ziqiu Tong, Lars Esser, Peter Galettis, Helmut Thissen, David Rudd, Christopher D. Easton, Azadeh Nilghaz, Bo Peng, Douer Zhu, Jennifer H. Martin, Nicolas H. Voelcker
Summary: This paper explores the use of a synthetic fluoropolymer, Teflon(TM) AF 2400, to functionalize organ-on-chip systems, reducing the absorption of hydrophobic compounds. Using CBD as a test compound, the researchers quantified CBD absorption into PDMS and found that surface modification with the fluoropolymer significantly increased CBD signals. The study also demonstrated the compatibility of fluoropolymer-modified microchannels for cell culture and CBD perfusion experiments.
Article
Chemistry, Multidisciplinary
Kartika Wardhani, Aviva Levina, Biyun Sun, Georges E. R. Grau, F. Richard Keene, J. Grant Collins, Peter A. Lay
Summary: Using a new photoluminescent polypyridylruthenium(II) stain, researchers have uncovered previously unknown interactions between extracellular vesicles (EVs) released from lipopolysaccharide-stimulated THP-1 monocytes and the blood-brain barrier (BBB). These findings shed light on the impact of bacteria-induced immune response on BBB and provide insights into human brain diseases.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Hao Zhe Yoh, Yaping Chen, Ali-Reza Shokouhi, Helmut Thissen, Nicolas H. Voelcker, Roey Elnathan
Summary: The advancement of nanofabrication technologies has enabled the exploration of new territories in nanoneedles applications, particularly in intracellular delivery. In this study, programmable polymeric nanotubes (NTs) were fabricated for efficient mRNA delivery into cells, and the role of actin cytoskeleton in this process was investigated.
Article
Chemistry, Inorganic & Nuclear
Liam J. J. Stephens, Elena Dallerba, Jenisi T. A. Kelderman, Aviva Levina, Melissa V. V. Werrett, Peter A. A. Lay, Massimiliano Massi, Philip C. C. Andrews
Summary: This study synthesized and characterized twelve Re(i) tricarbonyl diimine complexes with thiotetrazolato ligands. The tetrazolato ligand can bind to the Re(i) center through either the S atom or the N atom, with most complexes being bound to the N atom. In solution, the complexes exist as an equilibrating mixture of linkage isomers. The complexes exhibit photoluminescent properties, with emission wavelengths dependent on the nature of the diimine ligand.
DALTON TRANSACTIONS
(2023)
