Journal
PLOS ONE
Volume 11, Issue 3, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0147506
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Funding
- National Institutes of Health (NIH) Career Award [K25-AI65459]
- NIH [R15-GM094713]
- National Science Foundation Major Research Instrumentation [CHE-0722759]
- Maine Technology Institute MTAF [1106, 2061]
- University of Maine Vice President for Research
- Maine Economic Improvement Fund
- NIH Career Award [K25-AI65459, NIH R15-GM094713]
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Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of individual molecules over time. This information can allow for a dramatic increase in the number of different species simultaneously imaged in a sample, and can create super-resolution maps showing how single molecule emission spectra vary with position and time in a sample.
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