Article
Biochemistry & Molecular Biology
Dingding Lu, Ping Xu, Chengxiang Hou, Ruilin Li, Congwu Hu, Xijie Guo
Summary: In this study, the differential proteomes of silkworm in response to B. bassiana infection were analyzed using iTRAQ technology, revealing a large number of differentially expressed proteins. The lysozyme precursor, which was up-regulated at both the mRNA level and the protein level, played a crucial role in defending against B. bassiana infection.
MOLECULAR IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
T. Geng, F. Lu, H. Wu, D. Lou, N. Tu, F. Zhu, S. Wang
Summary: Insects use antifungal innate immunity as a defense mechanism against entomogenous fungi, with the Toll, Jak/STAT, and IMD signaling pathways being activated by the fungus Beauveria bassiana in silkworms. Inhibition of these pathways results in reduced antifungal activity in silkworm hemolymph. The study identified specific antifungal peptides regulated by each pathway, showing that antimicrobial peptide genes in B. mori have evolved through expansion and selection of existing genes to adapt to fungal challenges. This insight aids in understanding insect immune regulation mechanisms.
INSECT MOLECULAR BIOLOGY
(2021)
Article
Engineering, Chemical
Xiaoke Ma, Qi Ge, Rehab Hosny Taha, Keping Chen, Yi Yuan
Summary: The BbRib gene is involved in the infection process of B. bassiana and assists pathogenic fungi in escaping the insect host immune defense system. The study shows that the BbRib protein affects the growth and development of silkworms, leading to poisoning and physiological changes. This research provides insights into the potential molecular mechanism of BbRib protein toxicity towards insects and offers a foundation for the development of novel insecticidal toxins.
Article
Microbiology
Xueqin Mao, Dongxu Xing, Die Liu, Haoran Xu, Luyu Hou, Ping Lin, Qingyou Xia, Ying Lin, Guanwang Shen
Summary: In this study, the egt gene was successfully transformed into B. bassiana, leading to increased efficacy and coverage against silkworm larvae. These findings demonstrate the feasibility of improving entomopathogenic fungi using genetic engineering.
Article
Food Science & Technology
Nada Krasevec, Anastasija Panevska, Spela Lemez, Jaka Razinger, Kristina Sepcic, Gregor Anderluh, Marjetka Podobnik
Summary: Fungi are common pathogens of insects, with aegerolysin proteins playing a role in attack and defense processes, often linked to MACPF-like genes. Research has shown that BlyA interacts with insect-specific membrane lipids, potentially enhancing the entomopathogenic and antagonistic activity of Beauveria bassiana.
Article
Immunology
Gabriel Moura Mascarin, Natasha Sant'Anna Iwanicki, Jose Luis Ramirez, Italo Delalibera, Christopher A. Dunlap
Summary: Culturing Beauveria bassiana under high glucose concentrations and high aeration leads to a shift in fungal development towards blastospore production, with associated differential gene expression related to antioxidant, calcium transport, conidiation, and osmosensor-related genes. The highly expressed Hyd1 gene suggests involvement in spore coat assembly, and upregulation of HOG signaling pathway genes points to potential mechanisms behind blastospore development for industrial pest control applications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Zhaoming Dong, Qingyou Xia, Ping Zhao
Summary: Silkworms spin silk fibers to protect the pupa and have evolved various antimicrobial proteins in the cocoon. These proteins, including protease inhibitors and seroins, show stable structures and activities. While protease inhibitors play antifungal roles, seroins have broad-spectrum antimicrobial activities against bacteria, fungi and viruses. In addition, the silkworm cocoon also contains non-protein antimicrobial components. Understanding these antimicrobial cocoon components will help improve cocoon processing technology and expand their applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Lei Yu, Si-Yuan Xu, Xin-Cheng Luo, Sheng-Hua Ying, Ming-Guang Feng
Summary: The Rad4-Rad23-Rad33 complex plays a crucial role in nucleotide excision repair in budding yeast and has been rarely studied in filamentous fungi. Previous research identified that Rad23 is highly efficient in the photoreactivation of UV-impacted conidia in Beauveria bassiana due to its interaction with Phr2. This study discovered that Rad4A interacts with Rad23 in the nucleus and has no other role in B. bassiana's lifecycle, while Rad4B is functionally redundant. These findings shed light on the molecular basis of fungal adaptation to solar UV irradiation.
Article
Microbiology
Si-Yuan Xu, Lei Yu, Xin-Cheng Luo, Sheng-Hua Ying, Ming-Guang Feng
Summary: The white collar proteins WC1 and WC2 play a role in regulating conidiation capacity and conidial resistance to UVB irradiation in the insect-pathogenic fungus Beauveria bassiana. The null mutants of wc1 and wc2 showed reduced conidial yield, UVB resistance, and photoreactivation rate, but no significant impact on other lifecycle-related phenotypes. Transcriptomic analysis revealed overlapping roles of WC1 and WC2 in regulating fungal gene networks.
Article
Multidisciplinary Sciences
Chunlan Bu, Rui Zheng, Gaiqun Huang, Jianmei Wu, Gang Liu, Marion L. Donald, Tingfa Dong, Xiao Xu
Summary: The pairing of silkworms and mulberry trees based on their sex can affect the quality of cocoons and silk, with different feeding combinations leading to different outcomes.
Article
Microbiology
Spiridon Mantzoukas, Ioannis Lagogiannis, Dionusia Mpousia, Aristeidis Ntoukas, Katerina Karmakolia, Panagiotis A. Eliopoulos, Konstantinos Poulas
Summary: The endophytic fungus B. bassiana can successfully colonize V. vinifera plants and significantly impact root growth without causing harm to the plant.
Article
Microbiology
Yao Wang, Qi Fan, Dong Wang, Wei-Qiu Zou, De-Xiang Tang, Preeyanat Hongthong, Hong Yu
Summary: Beauveria is an important fungal resource and has economic and ecological value. Through surveying, 15 Beauveria species were collected and identified, including three new species. The research showed that some Beauveria isolates have potential in biocontrol of certain insects, while different isolates exhibited variations in pathogenicity and host specificity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Deepak Gola, Pankaj Kumar Tyagi, Nitin Chauhan, Anushree Malik, Sunil Kumar Srivastava
Summary: The presence of toxic pollutants in wastewater, such as dyes and heavy metals, poses risks to the environment and health. This study showed the potential of using the fungus Beauveria bassiana for removing Chromium and Indanthane blue, with higher efficiency in dye removal compared to Cr uptake.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Agronomy
Xu Qin, Xin Zhao, Shuaishuai Huang, Juan Deng, Xuebing Li, Zhibing Luo, Yongjun Zhang
Summary: Specific strains of Beauveria bassiana can be introduced as endophytes into host plants, promoting host plant growth, increasing resistance to microbial pathogens, and enhancing resistance to insect pests.
PEST MANAGEMENT SCIENCE
(2021)
Editorial Material
Microbiology
Almudena Ortiz-Urquiza
Summary: Fungal pathogenicity towards insects has evolved independently multiple times, resulting in specialist and generalist pathogens with varied lifestyles. The fungus Beauveria bassiana, with its diverse lifestyles as a saprophyte, pathogen, and symbiont, provides a unique system for studying host-pathogen interactions and fungal development. Addressing knowledge gaps and scrutinizing key players in ecological interactions involving B. bassiana will enhance our understanding of environmental microbiology and aid in developing new pest control methods.
Article
Entomology
Chenchen Lu, Zhiqing Li, Li Chang, Zhaoming Dong, Pengchao Guo, Guanwang Shen, Qingyou Xia, Ping Zhao
Article
Entomology
Lin Ye, Yan Zhang, Zhaoming Dong, Pengchao Guo, Dongchao Zhao, Haoyun Li, Hang Hu, Xiaofang Zhou, Haiqin Chen, Ping Zhao
Summary: The 30K proteins in silkworms play a crucial role in anti-fungal immunity by participating in cellular immune responses and recruiting hemocytes to the fungal surface, ultimately enhancing immune effectiveness.
Article
Biochemistry & Molecular Biology
Xiaolu Zhang, Yuhui Ni, Kaiyu Guo, Zhaoming Dong, Yuqing Chen, Hongtao Zhu, Qingyou Xia, Ping Zhao
Summary: Domestication of silkworm has altered gene expression patterns in the cocoon, with many protease inhibitors downregulated in domestic silkworms. The SPI51 gene structures and sequences differ between domestic and wild silkworms, affecting the function of protease inhibitors. Mutated forms of BmoSPI51 showed stronger antifungal activity, suggesting that active site and tail sequences play a key role in antifungal mechanisms.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Yutian Lei, Kaiyu Guo, Yan Zhang, Xiaolu Zhang, Lixia Qin, Xin Wang, Hongtao Zhu, Yuanyuan Guo, Wenxin Yang, Benchi Li, Qingyou Xia, Ping Zhao, Zhaoming Dong
Summary: This study reports the full-length sequence and structure of silkworm egg glue protein, revealing its adhesive properties and mechanism, as well as identifying two transglutaminases contributing to its high adhesion in the colleterial gland.
ACTA BIOMATERIALIA
(2021)
Article
Entomology
Zhaoming Dong, Lin Ye, Yan Zhang, Zhiyong Chen, Benchi Li, Tao Zhang, Ping Zhao
Summary: This study analyzed the glycosylation of serum proteins in silkworms and identified many glycoproteins involved in proteolysis and carbohydrate metabolic processes, providing important insights into the function of N-linked glycosylation in metabolism, immunity, and metamorphosis.
JOURNAL OF INSECT SCIENCE
(2021)
Article
Immunology
Zhaoming Dong, Lingna An, Mengyao Lu, Muya Tang, Haiqin Chen, Xuan Huang, Yong Hou, Guanwang Shen, Xiaolu Zhang, Yan Zhang, Qingyou Xia, Ping Zhao
Summary: SPINK7 plays a significant role in regulating immune defense in silkworms by recognizing fungal PAMP and promoting encapsulation, melanization, and other defense responses by hemocytes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Hong-Lei Li, Lin Wu, Zhaoming Dong, Yusong Jiang, Sanjie Jiang, Haitao Xing, Qiang Li, Guocheng Liu, Shuming Tian, Zhangyan Wu, Bin Wu, Zhexin Li, Ping Zhao, Yan Zhang, Jianmin Tang, Jiabao Xu, Ke Huang, Xia Liu, Wenlin Zhang, Qinhong Liao, Yun Ren, Xinzheng Huang, Qingzhi Li, Chengyong Li, Yi Wang, Baskaran Xavier-Ravi, Honghai Li, Yang Liu, Tao Wan, Qinhu Liu, Yong Zou, Jianbo Jian, Qingyou Xia, Yiqing Liu
Summary: This study presents a high-quality, chromosome-scale reference genome of the ginger 'Zhugen' assembled from PacBio long reads, Illumina short reads, and Hi-C reads. The genome was phased into two haplotypes, demonstrating excellent gene pair collinearity and differential expression between alleles. The study also identified a biosynthetic pathway and transcription factor networks for gingerol analogs biosynthesis.
HORTICULTURE RESEARCH
(2021)
Article
Materials Science, Biomaterials
Xin Wang, Xiaoyin Tan, Qingsong Liu, Yi Li, Xinning Li, Zhaoming Dong, Haonan Dong, Qingyou Xia, Ping Zhao
Summary: The mechanism of silk fiber formation in silkworms is still not fully understood, but studies have shown that V-ATPase plays a key role in maintaining the pH gradient and fine structure of the silk gland, as well as regulating the mechanical properties of silk fibers. Inhibiting V-ATPase activity can improve the mechanical properties of silk fibers.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Correction
Plant Sciences
Hong-Lei Li, Lin Wu, Zhaoming Dong, Yusong Jiang, Sanjie Jiang, Haitao Xing, Qiang Li, Guocheng Liu, Shuming Tian, Zhangyan Wu, Bin Wu, Zhexin Li, Ping Zhao, Yan Zhang, Jianmin Tang, Jiabao Xu, Ke Huang, Xia Liu, Wenlin Zhang, Qinhong Liao, Yun Ren, Xinzheng Huang, Qingzhi Li, Chengyong Li, Yi Wang, Baskaran Xavier-Ravi, Honghai Li, Yang Liu, Tao Wan, Qinhu Liu, Yong Zou, Jianbo Jian, Qingyou Xia, Yiqing Liu
HORTICULTURE RESEARCH
(2021)
Article
Engineering, Biomedical
Xin Wang, Xiaoqian Xie, Kang Xie, Qingsong Liu, Yi Li, Xiaoyin Tan, Haonan Dong, Xinning Li, Zhaoming Dong, Qingyou Xia, Ping Zhao
Summary: Chitin plays an important role in the spinning duct of silkworm, where it co-locates with cuticle proteins to form a multilayer structure in the cuticular layer. Knocking down the expression of cuticle proteins leads to disruption of the fine structure of the cuticular layer and negative impacts on the mechanical properties of silk.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Kaiyu Guo, Xiaolu Zhang, Dongchao Zhao, Lixia Qin, Wenchao Jiang, Wenbo Hu, Xiao Liu, Qingyou Xia, Zhaoming Dong, Ping Zhao
Summary: This study reports a novel sericin, sericin5 (Ser5), and describes its sequence, structure, and translation products in Bombyx mori. The research compares and analyzes the amino acid composition, repeat regions, hydrophilicity, and adhesive performances of sericins from cocoon silk and non-cocoon silk.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Zhaoming Dong, Qingyou Xia, Ping Zhao
Summary: Silkworms spin silk fibers to protect the pupa and have evolved various antimicrobial proteins in the cocoon. These proteins, including protease inhibitors and seroins, show stable structures and activities. While protease inhibitors play antifungal roles, seroins have broad-spectrum antimicrobial activities against bacteria, fungi and viruses. In addition, the silkworm cocoon also contains non-protein antimicrobial components. Understanding these antimicrobial cocoon components will help improve cocoon processing technology and expand their applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Lixia Qin, Jing Li, Kaiyu Guo, Mengyao Lu, Yan Zhang, Xiaolu Zhang, Yanqiong Zeng, Xin Wang, Qingyou Xia, Ping Zhao, Ai-bing Zhang, Zhaoming Dong
Summary: Animals use minerals and organic components to construct biomaterials. The cocoon of the oriental moth contains mineral crystals, silk fibers, and other organic matter. It is mainly composed of calcium oxalates and Asx/Ser/Gly-rich cocoon proteins. The findings provide important references for understanding the formation mechanism and mechanical properties of the oriental moth cocoon.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yueting Sun, Wenyu Shi, Quan Zhang, Haiqiong Guo, Zhaoming Dong, Ping Zhao, Qingyou Xia
Summary: In this study, the anti-inflammatory mechanism of sericin protein was investigated. It was found that the anti-inflammatory ability of sericin was positively correlated with the purity of sericin 1 protein. RNA-seq analysis showed that sericin inhibited the activation of PRRs signaling pathways, such as TLRs and NLRs, and suppressed the MyD88/NF-κB pathway, thereby reducing the expressions of inflammatory cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dong Cheng, Zhaoming Dong, Ping Lin, Guanwang Shen, Qingyou Xia
Summary: This study reveals the crucial role of acetylation modification and chromatin accessibility in the transcriptional activation of ecdysone-responsive genes during insect development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)