Article
Multidisciplinary Sciences
Hugo O. Valdivia, Priyaleela Thota, Greys Braga, Leonila Ricopa, Keare Barazorda, Carola Salas, Danett K. Bishop, Christie A. Joya
Summary: Gazelle, a novel rapid malaria diagnostic device, showed comparable diagnostic accuracy to light microscopy for detecting P. vivax infections and outperformed two rapid diagnostic tests, even in cases of low parasitemia infections.
Article
Biochemical Research Methods
Juxin Yin, Jizhi Tong, Jiale Li, Guangye Shao, Bo Xie, Jianjian Zhuang, Gang Bi, Ying Mu
Summary: We designed a portable and high-throughput nucleic acid detection device for rapid diagnosis. This device is small and lightweight, capable of running 16 samples simultaneously and providing stable and accurate temperature control. The results showed good linearity and coefficient of variation, and the device can detect as low as 10 copies of nucleic acid. Therefore, this portable device has advantages in high-throughput nucleic acid detection in the field, especially under resource-limited conditions.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Computer Science, Information Systems
Hyusim Park, Younghun Park, Shanthala Lakshminarayana, Hyun-Min Jung, Min-Yeong Kim, Kyu Hwan Lee, Sungyong Jung
Summary: This paper presents an all-in-one electroanalytical device (AED) which is a cost-effective, field-ready, and miniaturized electronic POC device integrated with the most commonly used electroanalytical techniques. The AED can analyze multiple electrochemical sensors simultaneously and has a small size and user-friendly interface.
Article
Chemistry, Analytical
Harpreet Singh, Nirmalya Halder, Baldeep Singh, Jaskaran Singh, Shrey Sharma, Yosi Shacham-Diamand
Summary: Precision agriculture is vital for food security in a growing global population, and assessing nutrients is crucial for data-driven agriculture. Insufficient soil nutrient assessment can threaten agricultural viability, but existing testing procedures are expensive and time-consuming. This research aims to assess soil nutrient levels by fabricating a novel colorimeter to provide critical information on restoring optimal soil fertility.
Review
Cell Biology
Yinxu Bian, Tao Xing, Kerong Jiao, Qingqing Kong, Jiaxiong Wang, Xiaofei Yang, Shenmin Yang, Yannan Jiang, Renbing Shen, Hua Shen, Cuifang Kuang
Summary: As the demand for bio-medical mobile workstations increases, there is a growing need for lightweight, low-cost microscopic imaging devices. High-performance computational portable microscopes with small sizes and low prices are suitable for fast, timely, and large-scale deployment.
Article
Biophysics
Fan-En Chen, Pei-Wei Lee, Alexander Y. Trick, Joon Soo Park, Liben Chen, Kushagra Shah, Heba Mostafa, Karen C. Carroll, Kuangwen Hsieh, Tza-Huei Wang
Summary: POC-CRISPR is a single-step CRISPR-Cas assisted assay that simplifies sample preparation through magnetic-based nucleic acid concentration and transport, enabling rapid detection of SARS-CoV-2. In clinical evaluation, POC-CRISPR successfully detected 27 positive samples, with 20 samples detecting high loads of SARS-CoV-2 within 20 minutes.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Biomedical
Yaw-Jen Chang, Shang-Fen Yeh, Pin-Jyun Chen
Summary: This paper presents a portable POCT device for simultaneous testing of ABO and Rh(D) blood typing and hemophilia diagnosis using only a small amount of whole blood sample. The device measures the degree of red blood cell agglutination and the concentration of factor VIII to determine the blood type and hemophilia. The device does not require expensive equipment and is suitable for quantitative testing in emergency situations or natural disasters.
BIOMEDICAL MICRODEVICES
(2023)
Article
Biotechnology & Applied Microbiology
Pei-Wei Lee, Marissa Totten, Liben Chen, Fan-En Chen, Alexander Y. Trick, Kushagra Shah, Hoan Thanh Ngo, Mei Jin, Kuangwen Hsieh, Sean X. Zhang, Tza-Huei Wang
Summary: Candida auris is an emerging multidrug-resistant fungal pathogen that has spread widely. Researchers have developed a POC device based on Droplet magnetofluidics technology to quickly detect the presence of C. auris.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
G. Papadakis, A. K. Pantazis, N. Fikas, S. Chatziioannidou, V Tsiakalou, K. Michaelidou, V Pogka, M. Megariti, M. Vardaki, K. Giarentis, J. Heaney, E. Nastouli, T. Karamitros, A. Mentis, A. Zafiropoulos, G. Sourvinos, S. Agelaki, E. Gizeli
Summary: A device for real-time colorimetric LAMP was developed, combining the accuracy of lab-based quantitative analysis with the simplicity of point-of-care testing, featuring rapid analysis, high quantification, crude sample-compatibility, low detection limit, smartphone operation, fast prototyping, and dye selection capabilities.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Electrical & Electronic
Vandana Jain, Raghvendra Kumar Dwivedi, K. Muralidhar
Summary: Electrowetting-on-Dielectric (EWOD) is a powerful technology for biological and chemical diagnostics. This study developed a closed EWOD device integrated with thermal cycling and optical diagnostics. The device achieves precise thermal cycling needed in clinical and medical diagnostics, and has demonstrated effectiveness in glucose detection and liquid drop transportation. It shows reliability and potential for low-cost point-of-care applications.
SENSORS AND ACTUATORS A-PHYSICAL
(2022)
Article
Metallurgy & Metallurgical Engineering
Guo Dong, Li Gen, Miao Jia-qi, Shen Ya-jing
Summary: This paper presents a smartphone-based portable urinalysis system that significantly improves the accuracy and stability of traditional colorimetric analysis methods for urine examination results. The system is easy to operate and can be used for rapid routine check-ups.
JOURNAL OF CENTRAL SOUTH UNIVERSITY
(2021)
Article
Chemistry, Analytical
Manpreet Kaur, Dorin Harpaz, Evgeni Eltzov
Summary: A gelatin-based colorimetric assay was developed for the rapid detection of Bacillus cereus in food. The assay is low-cost, simple, and portable, and has a comparable detection range to existing methods.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Antony Jesuraj, Umer Hassan
Summary: A portable 3D-printed multispectral spectrophotometer has been developed for real-time monitoring of enzyme molecular activity. The device shows significant correlation with measurements from a standard spectrophotometer. It has potential applications in absorbance spectroscopy-based point-of-care biomedical and healthcare.
Article
Biochemical Research Methods
Cyril Soliman, Dandan Tu, Samuel Mabbott, Gerard Cote, Kristen Maitland
Summary: This study developed a portable multi-modal spectroscopic platform capable of performing Raman and fluorescence measurements from a single sample site, which has potential in improving the sensitivity and robustness of biomarker detection.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Article
Chemistry, Analytical
Juan Garcia-Bernalt Diego, Pedro Fernandez-Soto, Sergio Marquez-Sanchez, Daniel Santos Santos, Begona Febrer-Sendra, Beatriz Crego-Vicente, Juan Luis Munoz-Bellido, Moncef Belhassen-Garcia, Juan M. Corchado Rodriguez, Antonio Muro
Summary: Nucleic acid amplification diagnostics, although sensitive and specific, lack speed, robustness, affordability, and user-friendliness. This study combines loop-mediated isothermal amplification technology with a handheld portable device (SMART-LAMP) to enable real-time nucleic acid amplification reactions through simple colorimetric measurements. The device is controlled by a dedicated smartphone app and can be used for diagnosing different infectious diseases, including Schistosomiasis, Strongyloidiasis, and COVID-19. The combination of long-term stabilized LAMP master mixes and the SMART-LAMP device improves point-of-care diagnosis of infectious diseases in low-resource settings, and can be easily adapted for diagnosing other infectious diseases.
Article
Biochemistry & Molecular Biology
Thirumal D. Kumar, Nikita Jain, Udhaya S. Kumar, Prangya Paramita Jena, Siva Ramamoorthy, George Priya C. Doss, Hatem Zayed
Summary: This study utilized computational pipelines to analyze missense mutations in the GALC gene, identifying R396L and R396W as the most deleterious and destabilizing to GALC. The impact of these mutations on the chaperone alpha lobeline was validated using molecular docking, showing R396W to have minimal binding affinity compared to R396L and native GALC.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Review
Chemistry, Medicinal
Emanuel L. Peter, Prakash B. Nagendrappa, Anita Kaligirwa, Patrick Engeu Ogwang, Crispin Duncan Sesaazi
Summary: Momordica charantia L. fruit and seed extracts have shown significant effects in reducing blood glucose levels and providing hepato-renal protective effects in animal models of type 2 diabetes mellitus. However, there is a call for future research to focus on standardizing extracts based on chemical markers and improving the overall quality of preclinical studies.
PHYTOTHERAPY RESEARCH
(2021)
Article
Plant Sciences
Mariadoss Alphonse, Rajasekaran Chandrasekaran, Siva Ramamoorthy, Devanand P. Fulzele, Ravindra Raina, Kalaivani Thiagarajan
Summary: The roots and shoots of Gentiana kurroo Royle are rich sources of gentiopicroside (GPD). The plant is traditionally used for curing many metabolic diseases. In vitro propagation may offer an alternative source of G. kurroo, and this study developed an effective in vitro shoot multiplication system with optimized medium compositions.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Oncology
Jayasindu Mathiyazhagan, Ramamoorthy Siva, Rama Jayaraj, Harishkumar Madhyastha, Gothandam Kodiveri Muthukaliannan
Summary: The study found that the Zingiber officinale-Terminalia chebula extract (ZOTC) has a suppressive effect on tumor progression in DMBA-induced breast cancer rats, potentially through the inhibition of the mTOR pathway.
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Sujithra Vijayakumar, Siva Ramamoorthy
Summary: A bright yellow pigment-producing endophytic fungus strain was isolated from the meristems of Delonix regia, identified as Fusarium solani. Analysis showed that the crude pigments were toxic and elevated cholesterol and glucose levels in rats. Rats treated with the pigments also showed damaged liver and kidney tissues, with an LD50 value higher than 1000 mg/kg.
INDIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Plant Sciences
Ressin Varghese, C. George Priya Doss, Raju Suresh Kumar, Abdulrahman Almansour, Natarajan Arumugam, Thomas Efferth, Siva Ramamoorthy
Summary: Phytopigments exhibit pleiotropic cardioprotective effects through various signaling pathways to alleviate cardiovascular diseases, suggesting their potential as novel and effective therapeutic candidates. More randomized, placebo-controlled clinical studies are recommended to establish the clinical efficacy of phytopigments.
Review
Plant Sciences
Janani Manochkumar, Anuma Singh, Thomas Efferth, Siva Ramamoorthy
Summary: Carotenoids have the potential to prevent and treat respiratory diseases by activating certain pathways and inhibiting others, resulting in anti-inflammatory and anti-tumor effects. In addition, epidemiological studies have shown that dietary intake of carotenoids can reduce the risk of lung diseases.
Review
Biochemistry & Molecular Biology
Raghul Senthilnathan, Ilamathi Ilangovan, Milind Kunale, Nalini Easwaran, Siva Ramamoorthy, Ashokkumar Veeramuthu, Gothandam Kodiveri Muthukaliannan
Summary: Gene editing techniques, especially CRISPR, have revolutionized the field of Genetic engineering due to their simplicity and effectiveness in editing a wide range of organisms. This review focuses on assessing the mutagenesis mediated by Cas12 systems and elucidating their editing efficacy in various species. Furthermore, it highlights the experimental benefits and applications of Cas12 systems.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Leepica Kapoor, S. Udhaya Kumar, Sourav De, Sujithra Vijayakumar, Nitin Kapoor, S. K. Ashok Kumar, C. George Priya Doss, Siva Ramamoorthy
Summary: This study evaluated the potential of bixin as an anti-skin aging agent and investigated its inhibitory mechanism on skin aging-related enzymes. Through a series of experiments, it was found that bixin possesses antioxidant activity and inhibitory effects on enzyme activity, while also having the ability to combat UVA-induced photoaging. Furthermore, the findings of this study lay a foundation for exploring the pharmaceutical applications of bixin in other biochemical pathways.
Review
Plant Sciences
Ressin Varghese, Thomas Efferth, Siva Ramamoorthy
Summary: Lung cancer, a leading malignancy worldwide, has been mitigated through the use of phytopigments such as carotenoids. While in vitro and animal studies have shown promising results, clinical trials have yielded contradictory outcomes, highlighting the need for further verification.
Review
Biochemistry & Molecular Biology
Ressin Varghese, Aswani Kumar Cherukuri, Nicholas H. Doddrell, C. George Priya Doss, Andrew J. Simkin, Siva Ramamoorthy
Summary: Improving photosynthesis is a promising approach to enhance food security, and machine learning can aid in analyzing large-scale photosynthetic data to increase crop yield by correlating hyperspectral data with photosynthetic parameters.
Article
Biochemistry & Molecular Biology
N. Madhana Priya, C. George Priya Doss, R. Magesh
Summary: This study focuses on discovering small compounds from natural compound libraries for the treatment of drug-resistant tuberculosis. Using pharmacophore-based virtual screening and molecular docking experiments, potential inhibitors of inhA and katG were identified. Molecular dynamics simulations confirmed the stability and compactness of the identified inhibitors.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Public, Environmental & Occupational Health
Leepica Kapoor, Siva Ramamoorthy
Summary: This study shows that Bixin has a significant impact on the body composition of photoaged mice, increasing lean mass and reducing fat mass. Therefore, Bixin may be used as an antiadipaging nutraceutical.
CLINICAL EPIDEMIOLOGY AND GLOBAL HEALTH
(2022)
Proceedings Paper
Materials Science, Multidisciplinary
Phani Kumar Pullela, Swathi Korrapati, K. Sharan Teja Reddy, Vijayalakshmi Uthirapathy
Summary: This article presents a zero-cost method for concentrating gold nanoparticle solution using sodium polyacrylate beads. It effectively reduces the cost of lateral flow technologies by achieving nanoparticle concentration with minimal expense.
MATERIALS TODAY-PROCEEDINGS
(2022)
Proceedings Paper
Materials Science, Multidisciplinary
M. Chandrappa, Korrapati Swathi, S. Girish Kumar, Phani Kumar Pullela
Summary: Quinolines are used as important chemical entities in medicinal chemistry, particularly as a last line of therapy for bacterial and viral infections. The use of Fe3O4@SiO2 nanoparticles enhances the yield of quinoline synthesis, especially for heterocyclic compounds.
MATERIALS TODAY-PROCEEDINGS
(2021)