Article
Multidisciplinary Sciences
Lin Zhu, Jingyi Li, Yanmei Dong
Summary: The study demonstrates that mesoporous bioactive glass (MBG) can release Si and Ca ions, has strong mineralization activity, and co-culture with human dental pulp cells can promote the expression of dentin matrix protein and alkaline phosphatase. After 4 weeks of subcutaneous implantation in nude mice, the formation of hard tissue with a structure similar to dentin tubules is observed.
Article
Biotechnology & Applied Microbiology
Min Wang, Yi Guo, Zexing Deng, Peng Xu
Summary: In this paper, a novel organic-inorganic composite hydrogel was designed, which exhibited excellent mechanical properties and promoted stem cell osteogenic differentiation, showing potential application in bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Edward J. Calabrese, Evgenios Agathokleous, Gaurav Dhawan, Rachna Kapoor, Vittorio Calabrese
Summary: This study represents the first assessment of hormetic dose responses by human dental pulp stem cells, focusing on cell renewal and differentiation. The study found that hormetic dose responses are commonly observed in this model, involving a wide range of chemicals including pharmaceuticals, dietary supplements/extracts from medicinal plants, and endogenous agents. The study evaluated the mechanistic foundations, study design considerations, and therapeutic implications of the hormetic dose responses in hDPSCs.
AGEING RESEARCH REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Lysann M. Kroschwald, Felix Allerdt, Anne Bernhardt, Sandra Rother, Kai Zheng, Iram Maqsood, Norbert Halfter, Christiane Heinemann, Stephanie Moeller, Matthias Schnabelrauch, Michael C. Hacker, Stefan Rammelt, Aldo R. Boccaccini, Vera Hintze
Summary: The study demonstrates that aECM containing BGN can promote osteogenic differentiation of hMSC while delaying cellular growth, with a stimulating effect on ALP activity and calcium deposition. Some donors show a synergistic effect between sGAG and BGN, contributing to bone regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Danijela Marovic, Havard J. Haugen, Visnja Negovetic Mandic, Matej Par, Kai Zheng, Zrinka Tarle, Aldo R. Boccaccini
Summary: Experimental dental resin composites incorporating copper-doped mesoporous bioactive glass nanospheres were found to have good mechanical properties and photopolymerization, with Cu-MBGN composite with 1% and 5% concentrations showing the highest values for flexural strength, flexural modulus, microhardness, and degree of conversion after 28 days. The addition of Cu-MBGN did not significantly affect flexural strength and microhardness of the composite materials with silica fillers, indicating potential for clinical implementation.
Article
Biotechnology & Applied Microbiology
Nunthawan Nowwarote, Stephane Petit, Francois Come Ferre, Florent Dingli, Victor Laigle, Damarys Loew, Thanaphum Osathanon, Benjamin P. J. Fournier
Summary: The study identified the matrisome of ECM proteins derived from human DPSCs and found that it can regulate mineralization, even in cells which do not respond to mineralization assaults like GF.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Rosanna Di Tinco, Ugo Consolo, Alessandra Pisciotta, Giulia Orlandi, Giulia Bertani, Milena Nasi, Jessika Bertacchini, Gianluca Carnevale
Summary: This study evaluated the effects of three different bone substitute biomaterials (BSBs) on human dental pulp stem cells (hDPSCs). The results showed that the BSBs did not alter cell proliferation, stemness markers expression, or induce any inflammatory responses. Furthermore, the formulation of the BSBs also influenced the biological properties of the stem cells.
Article
Biochemistry & Molecular Biology
Wang Wang, Ming Yan, Ghazal Aarabi, Ulrike Peters, Marcus Freytag, Martin Gosau, Ralf Smeets, Thomas Beikler
Summary: The study shows that the new cryopreservation method is effective in preserving hDPSCs without compromising their capabilities and stemness. The cells maintain excellent proliferation and differentiation abilities, which are crucial for cell therapy and dental bank establishment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Fabian Westhauser, Simon Decker, Qaisar Nawaz, Felix Rehder, Sebastian Wilkesmann, Arash Moghaddam, Elke Kunisch, Aldo R. Boccaccini
Summary: Mesoporous bioactive glass nanoparticles (MBGNs) have been studied for their potential in bone tissue engineering, with 5Zn-MBGNs showing the most favorable pro-osteogenic properties in terms of cellular differentiation, ECM formation, and calcification.
Article
Cell & Tissue Engineering
Wen Kang, Yiwen Wang, Jiaying Li, Weige Xie, Dan Zhao, Li Wu, Hongwei Wang, Sijing Xie
Summary: This study found that TAS2R was significantly upregulated in inflammatory dental pulp tissues, and the expression of TAS2R subtypes increased in hDPSCs under stimulation. TAS2R promoted odontoblastic differentiation of hDPSCs by mediating the increase in intracellular Ca2+.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Anastasia Kotova, Arseniy A. Lobov, Julia A. Dombrovskaya, Valentina Y. Sannikova, Nadezhda A. Ryumina, Polina Klausen, Alexey L. Shavarda, Anna B. Malashicheva, Natella Enukashvily
Summary: Dental stem cells, derived from neural crest stem cells, exhibit differences in functional and biological properties, including neural markers, cell morphology, proliferation rate, and osteogenic differentiation capabilities. Dental pulp stem cells and periodontal ligament stem cells show distinctions in neural markers, nuclear protein expression, proliferation rate, etc.
Article
Cell Biology
Francesca Santilli, Jessica Fabrizi, Stefano Martellucci, Costantino Santacroce, Egidio Iorio, Maria Elena Pisanu, Mattea Chirico, Loreto Lancia, Fanny Pulcini, Valeria Manganelli, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This study analyzed the lipid composition of human Dental Pulp-Derived Stem Cells (DPSCs) and investigated the role of lipid rafts during multilineage differentiation. The results showed that mono-unsaturated fatty acids were the most representative species in the acyl chains of DPSCs. Moreover, disrupting lipid rafts prevented AKT activation and lineage-specific mRNA expression during multilineage differentiation, indicating the importance of lipid raft integrity in DPSCs differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Chiyu Li, Fanqi Meng, Zhijie Yang, Jiang Peng, Yuwei Guo, Jing Na, Qiusheng Shi, Yu Liu, Yu Wang, Lisha Zheng, Yubo Fan
Summary: A micropattern-based system is developed to train dental pulp stem cells and enhance their stemness and differentiation potential. Micropatterns remodel cellular structures, induce immature mitochondrial state, and activate specific signaling pathways to enhance stemness. In vivo implantation proves the effectiveness of this approach in promoting nerve regeneration.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
L. Yao, F. Li, C. Yu, H. Wang, Y. Wang, L. Ye, F. Yu
Summary: The influence of chronological and replicative senescence on CD51(+)/PDGFR-alpha(+) hDPSCs was investigated using various experimental methods. Chronological senescence led to decreased CD51(+)/PDGFR-alpha(+) hDPSCs, impaired self-renewal, and higher ossificatory differentiation. Replicative senescence resulted in decreased CD51 expression, upregulated PDGFR-alpha expression, and weakened self-renewal and osteogenic differentiation. These findings highlight the distinct outcomes and core dysfunction of chronological and replicative senescence in CD51(+)/PDGFR-alpha(+) hDPSCs.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Kangrui Zeng, Weiping Li, Qiongyi Kang, Yutong Li, Qing Cheng, Wenwei Xia
Summary: This study revealed that miR-342-5p inhibits the odonto/osteogenic differentiation of hDPSCs by interfering with Wnt/beta-catenin signaling via targeting Wnt7b.
Review
Pharmacology & Pharmacy
Chinmaya Mahapatra, Ruda Lee, Manash K. Paul
Summary: This article discusses the interactions between nanomaterials and different types of organoids, covering various aspects such as organoid matrices, cargo delivery, bioprinting, therapeutic implications, etc.
DRUG DISCOVERY TODAY
(2022)
Article
Chemistry, Physical
T. Yadav, A. K. Vishwkarma, G. Brahmachari, I. Karmakar, P. Yadav, S. Kumar, C. Mahapatra, J. Chowdhury, R. Kumar, G. N. Pandey, P. K. Tripathi, A. Pathak
Summary: In this study, optimization of a bio-relevant molecule and its monohydrate cluster was performed, resulting in the identification of three stable conformers. The influence of a water molecule on the energy and vibrational modes of the target molecule was investigated, and the electronic structures and vibrational spectra of the conformers were computed. The obtained theoretical results were validated by comparing them with experimental data. Additionally, Raman spectra were calculated for all conformers, and electronic structures, bond energies, and other properties were determined through NBO calculations.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Cell Biology
Huong Thu Vu, Ji-Young Yoon, Jae-Hee Park, Hae-Hyoung Lee, Khandmaa Dashnyam, Hae-Won Kim, Jung-Hwan Lee, Ji-Sun Shin, Jong-Bin Kim
Summary: This study found that hGF-CM can significantly enhance the proliferation, migration capability, and cell viability of DPSC, and promote odontogenic differentiation. The ERK pathway was identified as the key mechanism involved.
Article
Pharmacology & Pharmacy
Min-Kyung Jung, So-Chung Park, Yu-Jin Kim, Jong-Tae Park, Jonathan C. Knowles, Jeong-Hui Park, Khandmaa Dashnyam, Soo-Kyung Jun, Hae-Hyoung Lee, Jung-Hwan Lee
Summary: A calcium silicate root canal sealer reinforced with bioactive glass nanoparticles was developed and analyzed in terms of its physicochemical features and biological effects. The results showed that the sealer met the standards for root canal sealers, exhibited antimicrobial activity, and promoted osteogenic differentiation.
Article
Nanoscience & Nanotechnology
Xin Zheng Liu, Zhi Juan Wen, Yun Meng Li, Wan Ru Sun, Xiao Qian Hu, Jia Zhi Zhu, Xin Yu Li, Ping Yu Wang, Jose Luis Pedraz, Jung-Hwan Lee, Hae-Won Kim, Murugan Ramalingam, Shuyang Xie, Ranran Wang
Summary: In this study, a drug-free bacteria-derived outer membrane vesicle (OMV) functionalized Fe3O4-MnO2 (FMO) nanoplatform was developed for neutrophil-mediated targeted delivery and photothermally enhanced cancer immunotherapy. The modified OMVs enhanced the accumulation of FMO NPs at the tumor site and induced immunogenic cell death (ICD) and regulated the tumor hypoxia environment. Additionally, OMVs effectively activated immune cells and the photothermal therapy (PTT) caused by MnO2 and Fe3O4 stimulated systemic immunity and promoted the enrichment of neutrophil-equipped nanoparticles. This multi-modal treatment system with targeted delivery capability achieved effective tumor immunotherapy and prevention of tumor recurrence.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Materials Science, Multidisciplinary
Chung-Sung Lee, Rajendra K. Singh, Hee Sook Hwang, Na-Hyun Lee, Amal George Kurian, Jung-Hwan Lee, Hye Sung Kim, Min Lee, Hae-Won Kim
Summary: Severe injuries and diseases of bone tissue pose challenges for complete recovery. Materials-based nanotherapeutics can address issues related to therapeutic molecule delivery, ultimately improving effectiveness and reducing off-target concerns.
PROGRESS IN MATERIALS SCIENCE
(2023)
Article
Engineering, Environmental
Yu-Meng Li, Kapil D. Patel, Yoon-Koo Han, Suk-Min Hong, Yu-Xuan Meng, Hae-Hyoung Lee, Jeong Hui Park, Jonathan C. Knowles, Jung Keun Hyun, Jung-Hwan Le, Hae-Won Kim
Summary: Diseased or injured nervous systems have limited self-repair and regenerative capability, leading to lifelong disability and even death. Biomaterial scaffolds with specific properties, such as electroconductivity, can guide the selective differentiation of neural stem/progenitor cells (NSPCs) into neurons and oligodendrocytes. Electroconductive and mechano-competent scaffolds made of shape-memory polyurethane-polycaprolactone (PUCL) nanofibers and carbon nanotubes (CNTs) were prepared and showed promising potential for neural tissue regeneration.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Soo-Jin Chang, Yu-Jin Kim, Huong Thu Vu, Ji-Myung Choi, Jeong-Hui Park, Seong-Jin Shin, Khandmaa Dashnyam, Jonathan C. Knowles, Hae-Hyoung Lee, Soo-Kyung Jun, Mi-Ran Han, Joon-Haeng Lee, Jong-Soo Kim, Ji-Sun Shin, Jong-Bin Kim, Jung-Hwan Lee
Summary: This study investigated the effects of different viscosities of silicone oil on the properties of a sodium iodide paste. Six different paste groups were created and evaluated using various parameters. The D30L group showed superior outcomes compared to the conventional iodoform group, including reduced osteoclast formation. The optimized viscosity of the sodium iodide paste (D30L) may lead to favorable outcomes when used in primary teeth.
Article
Pharmacology & Pharmacy
Yu-Ji Jang, Yu-Jin Kim, Huong Thu Vu, Jeong-Hui Park, Seong-Jin Shin, Khandmaa Dashnyam, Jonathan C. C. Knowles, Hae-Hyoung Lee, Soo-Kyung Jun, Mi-Ran Han, Joon-Haeng Lee, Jong-Soo Kim, Jong-Bin Kim, Jung-Hwan Lee, Ji-Sun Shin
Summary: Calcium silicate-based cement (CSC) is widely used in dentistry due to its excellent biocompatibility, sealing ability, and antibacterial activity. This study compared four commercial CSCs and found that premixed CSCs had improved physical properties, with ECPR exhibiting the highest antibacterial properties. However, there were no significant differences in biological properties at 12.5% dilution. Further investigation is needed for clinical situations.
Article
Materials Science, Multidisciplinary
Dilip Kumar Chandra, Awanish Kumar, Chinmaya Mahapatra
Summary: This article reports the rapid production method of cerium oxide microtubes (CeO2-MT) and their cytocompatibility with C2C12 cells for the first time. The results show that these microtubes have high antioxidant activity and can be used as novel biomaterials for potential muscle tissue regeneration.
EMERGENT MATERIALS
(2023)
Article
Chemistry, Physical
T. Yadav, A. K. Vishwkarma, M. Mandal, I. Karmakar, A. Pathak, G. Brahmachari, P. K. Tripathi, A. K. Maddheshiya, N. P. Yadav, C. Mahapatra
Summary: The structural and spectroscopic analysis of a warfarin derivative was performed, and low-lying energy conformations of this compound were found through one-dimensional potential energy scanning. The potential energy distributions and vibrational spectra of the most stable conformer were calculated and compared with experimental results. This investigation provides crucial information for further studies on similar compounds.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Environmental Sciences
Dilip Kumar Chandra, Awanish Kumar, Chinmaya Mahapatra
Summary: This study presents a simple approach for producing high-performance biodegradable plastic from plant-based resources using in situ lignin regeneration. The cellulose-chitosan slurry is formed by the deconstruction of leaves collected from plants, and cellulose bioplastic materials are produced by hydrogen bonding. The fabricated bioplastics exhibit excellent mechanical properties, water stability, and thermal stability. Additionally, lignocellulosic bioplastic has a lower environmental impact due to its simple recycling or secure biodegradability.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Chemistry, Multidisciplinary
Sung-Min Park, Jung-Hwan Lee, Kwang Sung Ahn, Hye Won Shim, Ji-Young Yoon, Jeongeun Hyun, Jun Hee Lee, Sunyoung Jang, Kyung Hyun Yoo, Yoon-Kwan Jang, Tae-Jin Kim, Hyun Kyu Kim, Man Ryul Lee, Jun-Hyeog Jang, Hosup Shim, Hae-Won Kim
Summary: Cyclic-stretching enhances the efficiency of cellular reprogramming by activating mechanosensitive molecules and inducing epigenetic modifications.
Review
Materials Science, Multidisciplinary
Dilip Kumar Chandra, Rui L. Reis, Subhas C. Kundu, Awanish Kumar, Chinmaya Mahapatra
Summary: Carbon nanotubes (CNTs) offer significant potential in antifungal drug delivery. Their unique physicochemical properties and the development of functionalized CNTs and CNT-based hybrid materials (CNT-HMs) enable precise drug delivery, bio-barrier penetration, controlled release, and improved antifungal efficacy against fungal cells. This review examines CNTs and CNT-HMs in antifungal drug delivery, covering functionalization, drug loading, efficacy, and toxicity mitigation.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Cell & Tissue Engineering
Yongwen Guo, Mengting He, Peiqi Wang, Ding Bai, Jeong-Hui Park, Khandmaa Dashnyam, Jung-Hwan Lee, Olivier Huck, Nadia Benkirane-Jessel, Hae-Won Kim, Murugan Ramalingam
Summary: The combined use of dental follicle cells, treated dentin matrix (TDM), and Hertwig's epithelial root sheath (HERS) cells showed promising results in regenerating periodontal structures in a nondental microenvironment.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)