Article
Veterinary Sciences
Sang-Ik Oh, Suk-Han Jung, Han-Kyoung Lee, Changyong Choe, Tai-Young Hur, Kyoung-Min So
Summary: Giardia duodenalis infection was found in 5.6% of Korean native calves, with higher prevalence in calves aged >= 1 month and experiencing watery diarrhea. The majority of positive samples belonged to assemblage E, while a few belonged to zoonotic assemblage A, emphasizing the importance of continuous surveillance of genetic mutations in G. duodenalis.
VETERINARY SCIENCES
(2021)
Article
Immunology
Ho Yin Pekkle Lam, Tina Tu-Wen Chen, Yu-Chuan Tseng, Kai-Chih Chang, Ting-Hua Yang, Shih-yi Peng
Summary: The first epidemiological investigation of G. duodenalis infection in animals in Hualien, Taiwan was conducted, revealing infection rates of 19.87% for cattle and 4.26% for pigs. Assemblage A was detected in pigs, indicating potential zoonotic transmission.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Veterinary Sciences
Zhao-Jun Heng, Jian-Fa Yang, Xin-Yan Xie, Cui-Rong Xu, Jun-Rong Chen, Jun Ma, Jun-Jun He, Hua-Ming Mao
Summary: This study investigated the occurrence and genetic diversity of Giardia duodenalis in Holstein cattle in Yunnan Province, China. The results showed a high infection rate in preweaned calves, with assemblage E being the predominant group. Mixed infection of assemblages A and E was also detected in three samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Zhaohui Cui, Qilin Wang, Xiyao Huang, Jiayi Bai, Bingyang Zhu, Bingchen Wang, Xiaohang Guo, Meng Qi, Junqiang Li
Summary: The prevalence and genetic diversity of G. duodenalis in captive alpine musk deer were investigated in this study. The overall prevalence was 19.3% with two genetic assemblages identified. Six novel assemblage A multilocus genotypes were discovered through multilocus genotyping analysis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gianluca Marucci, Ilaria Zullino, Lucia Bertuccini, Serena Camerini, Serena Cecchetti, Agostina Pietrantoni, Marialuisa Casella, Paolo Vatta, Alex D. Greenwood, Annarita Fiorillo, Marco Lalle
Summary: This study conducted high-throughput sequencing of four GLV strains from Giardia isolates of human and animal origin, and identified a new, unclassified viral sequence unrelated to Giardiavirus. The experimental results challenge the current knowledge on GLV, revealing the presence of at least two GLV subtypes with different phenotypes and transmissibility.
Article
Microbiology
Judith Diaz-Garcia, Ana Gomez, Luis Alcala, Elena Reigadas, Carlos Sanchez-Carrillo, Ana Perez-Ayala, Elia Gomez-Garcia de la Pedrosa, Fernando Gonzalez-Romo, Paloma Merino-Amador, Maria Soledad Cuetara, Coral Garcia-Esteban, Inmaculada Quiles-Melero, Nelly Daniela Zurita, Maria Munoz-Algarra, Isabel Sanchez-Romero, Maria Teresa Duran-Valle, Aida Sanchez-Garcia, Eva Alcoceba, Patricia Munoz, Pilar Escribano, Jesus Guinea
Summary: This study monitored antifungal resistance in Candida parapsilosis isolates from inpatients at hospitals in the Madrid metropolitan area over a three-year period. The findings reveal the spread of fluconazole-resistant genotypes of C. parapsilosis across multiple hospitals in Madrid, Spain.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Parasitology
Ajanta Ghosal, Sanjib K. Sardar, Tapas Haldar, Maimoon Maruf, Yumiko Saito-Nakano, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly
Summary: The genetic diversity of Giardia duodenalis in eastern India was studied and two distinct assemblages were found, with prevalence rates of 30.8% and 63.5% respectively. The study emphasizes the importance of implementing control measures due to the high prevalence of the parasite, especially among children. The presence of numerous unique genotypes in this region provides valuable data for future research.
PARASITOLOGY RESEARCH
(2023)
Article
Pediatrics
Carolina Hernandez-Castro, Alejandro Dashti, Amoolya Vusirikala, Sooria Balasegaram, Pamela Carolina Koster, Begona Bailo, Elena Imana, Andrea Lopez, Maria Teresa Llorente, David Gonzalez-Barrio, Sergio Sanchez, David Carmena
Summary: Giardia duodenalis and Blastocystis sp. were commonly found in toddlers attending day-care centres in Central Spain. This study identified the genetic diversity and temporal dynamics of these two eukaryotic parasites, and found that they can persist in some children for a long period of time.
EUROPEAN JOURNAL OF PEDIATRICS
(2023)
Article
Agriculture, Dairy & Animal Science
Fu-Rong Zhao, Ning Zhang, Wen-Yuan Miao, Ran Wu, Lin-Lin Cui, Cui-Qin Huang, Dong-Hui Zhou
Summary: This study investigated the prevalence and genotypes of G. duodenalis in pigs in Fujian Province, China. The findings revealed that the pigs in Fujian Province mainly carried the E genotype of G. duodenalis, and highlighted the potential zoonotic risk of G. duodenalis infection from pigs to humans in this area.
Article
Veterinary Sciences
Penglin Wang, Ling Zheng, Linke Liu, Fuchang Yu, Yichen Jian, Rongjun Wang, Sumei Zhang, Longxian Zhang, Changshen Ning, Fuchun Jian
Summary: The study revealed that sheep and goats could be potential reservoir hosts for three gastrointestinal pathogens. The research provided a platform for further detailed genotyping or subtyping of intestinal pathogens to enhance understanding of their risks and modes of transmission.
BMC VETERINARY RESEARCH
(2022)
Article
Microbiology
Pamela Carolina Koster, Antonio F. Malheiros, Jeffrey J. Shaw, Sooria Balasegaram, Alexander Prendergast, Heloise Lucaccioni, Luciana Melhoranca Moreira, Larissa M. S. Lemos, Alejandro Dashti, Begofia Bailo, Arlei Marcili, Herbert Sousa Soares, Solange Maria Gennari, Rafael Calero-Bernal, David Gonzalez-Barrio, David Carmena
Summary: Little information is available on the occurrence and genetic variability of the diarrhoea-causing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey found that G. duodenalis infection rates were positively linked to younger age and tribe in Tapirape people in the Brazilian Amazon. Children were the main group infected, showing high genetic diversity.
Article
Agriculture, Dairy & Animal Science
Pamela Carolina Koster, Begona Bailo, Alejandro Dashti, Carolina Hernandez-Castro, Rafael Calero-Bernal, Francisco Ponce-Gordo, David Gonzalez-Barrio, David Carmena
Summary: In remote or resource-limited areas where the cold chain cannot be maintained, it is challenging to store and preserve biological samples like faecal specimens. This study evaluated the suitability of three commercially available filter cards for long-term storage of faecal samples containing common diarrhoea-causing enteric protozoan parasites, showing that these cards can be compatible with molecular methods for up to six months at room temperature.
Article
Food Science & Technology
Maria Trelis, Sandra Saez-Duran, Pablo Puchades, Nicole Castro, Ana Miquel, Monica Gozalbo, Marius Vicent Fuentes
Summary: The study assessed the occurrence of Giardia duodenalis and Cryptosporidium spp. cysts in green leafy vegetables marketed in Valencia, Spain, and found a higher level of contamination in organic vegetables, highlighting the need for extreme hygiene measures.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Parasitology
Huan Tang, Yonggang Ye, Runmin Kang, Jifeng Yu, Ye Cao
Summary: This study investigated the infection status of Giardia duodenalis in rabbits from Shaanxi Province, PR China, with a total prevalence of 3.54%. Two predominant assemblages, B and E, were identified, with B being the main assemblage. There were no significant differences related to breeds, farms, and age groups.
Article
Parasitology
Yang Zou, Xiao-Dong Li, Yu-Meng Meng, Xiao-Long Wang, Hao-Ning Wang, Xing-Quan Zhu
Summary: This study investigated the prevalence and genotypes of Giardia duodenalis in zoo animals in three cities in China. Results showed that region and animal category were risk factors for infection, and multiple genotypes of G. duodenalis were identified in the animals, indicating genetic variability within zoo populations and a potential source of human infection.
PARASITOLOGY RESEARCH
(2022)
Letter
Chemistry, Multidisciplinary
Francisco M. Fernandez
Summary: This comment argues that the He-Laplace variational iteration method is equivalent to finding the Taylor series of a solution to a partial differential equation, and that using the textbook power series method is simpler and more efficient. Additionally, it suggests that the time series of a solution is not suitable for analyzing nonlinear problems in chemical kinetics and population dynamics, which require expressions valid for sufficiently large time. Furthermore, it criticizes the application of the method to tailor-made toy problems with known exact solutions, which do not seem to have any real-world physical applications.
JOURNAL OF MATHEMATICAL CHEMISTRY
(2022)
Letter
Chemistry, Multidisciplinary
Francisco M. Fernandez
Summary: The recent application of the Nikiforov-Uvarov (NU) method to an N-dimensional anharmonic oscillator with a central-field sextic potential-energy function is analyzed. Errors and incorrect interpretations in the derived equations by the author are pointed out. Exact particular solutions to the Schrodinger equation are obtained using the Frobenius method and compared with those obtained from the NU method.
JOURNAL OF MATHEMATICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Francisco M. Fernandez
Summary: We analyze the distribution of eigenvalues of the quantum-mechanical rotating harmonic oscillator using the Frobenius method. A suitable approach leads to a three-term recurrence relation for expansion coefficients, and truncation of the series provides exact analytical forms of some particular eigenvalues and eigenfunctions. The former can be organized to obtain useful information about the entire spectrum of the model.
JOURNAL OF MATHEMATICAL CHEMISTRY
(2022)
Editorial Material
Multidisciplinary Sciences
Francisco M. Fernandez
Summary: It is argued in this paper that recent conclusions about a doubly anharmonic oscillator are incorrect, and that there are bound states when the angular velocity of the rotating reference frame is null, contrary to what the authors claimed. The radial eigenvalue equation is conditionally solvable, and the derived analytical eigenvalues for states with polynomial solutions are affected by the approach used to obtain them.
PROCEEDINGS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
(2022)
Article
Infectious Diseases
Antonio Rivero-Juarez, Alejandro Dashti, Monica Santin, Pamela C. Koster, Pedro Lopez-Lopez, Maria A. Risalde, Ignacio Garcia-Bocanegra, Jose Carlos Gomez-Villamandos, Javier Caballero-Gomez, Mario Frias, Begona Bailo, Sheila Ortega, Aly Salimo Muadica, Rafael Calero-Bernal, David Gonzalez-Barrio, Antonio Rivero, Veronica Briz, David Carmena
Summary: The study demonstrates that pigs infected with Cryptosporidium spp. have a higher prevalence of HEV, indicating a potential correlation between certain enteroparasites and HEV infection. Moreover, animals carrying E. bieneusi appear to be more susceptible to HEV infection. In addition, the presence of intracellular versus extracellular enteroparasites in swine appears to have variable effects on the risk of HEV acquisition.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Editorial Material
Microbiology
Pamela C. Koester, David Gonzalez-Barrio, David Carmena
Article
Veterinary Sciences
Pamela C. Koster, Eva Martinez-Nevado, Andrea Gonzalez, Maria T. Abello-Poveda, Hugo Fernandez-Bellon, Manuel de la Riva-Fraga, Bertille Marquet, Jean-Pascal Guery, Tobias Knauf-Witzens, Annika Weigold, Alejandro Dashti, Begona Bailo, Elena Imana, Aly S. Muadica, David Gonzalez-Barrio, Francisco Ponce-Gordo, Rafael Calero-Bernal, David Carmena
Summary: This study assessed the occurrence, genetic diversity, and zoonotic potential of several intestinal protist species in captive non-human primates and their zookeepers from European zoological gardens. The results showed the presence of these protist species in both NHP and human samples, with significant variation in occurrence rates among different host species, sampling periods, and zoo institutions.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Ana M. Figueiredo, Alejandro Dashti, Monica Santin, Pamela C. Koster, Rita T. Torres, Carlos Fonseca, Atle Mysterud, Joao Carvalho, Pedro Sarmento, Nuno Neves, Dario Hipolito, Josman D. Palmeira, Daniela Teixeira, Catia Lima, Rafael Calero-Bernal, David Carmena
Summary: The occurrence and genetic diversity of Enterocytozoon bieneusi in wildlife and domestic animals in Portugal were investigated. It was found that wild carnivores and ungulates may act as reservoirs of zoonotic genotypes of E. bieneusi, posing a potential risk of infection to humans and domestic animals. This study is the first and largest molecular-based epidemiology survey carried out in Portugal to date.
Article
Public, Environmental & Occupational Health
Laura Rego, Sabrina Castro-Scholten, Carmen Cano, Debora Jimenez-Martin, Pamela C. Koster, Javier Caballero-Gomez, Begona Bailo, Alejandro Dashti, Carolina Hernandez-Castro, David Cano-Terriza, Fatima Vioque, Jenny G. Maloney, Monica Santin, Ignacio Garcia-Bocanegra, David Carmena, David Gonzalez-Barrio
Summary: Wild lagomorphs, such as rabbits and hares, can carry bacterial and parasitic diseases that can be transmitted to humans. In this study, fecal samples from wild rabbits and hares in southern Spain were examined for the presence of eukaryotic pathogens. The results showed that Giardia duodenalis was the most prevalent, followed by Cryptosporidium spp., Blastocystis sp., and Encephalitozoon intestinalis. These findings suggest a potential public health risk.
ZOONOSES AND PUBLIC HEALTH
(2023)
Article
Agriculture, Dairy & Animal Science
Sergio Santos-Silva, Danny Franciele da Silva Dias Moraes, Pedro Lopez-Lopez, Josman D. Palmeira, Rita T. Torres, Maria Sao Jose Nascimento, Alejandro Dashti, David Carmena, Antonio Rivero-Juarez, Joao R. Mesquita
Summary: The study found the presence of potentially zoonotic protozoa and Hepatitis E virus (HEV) in wild boar populations in Portugal. Parasites such as Cryptosporidium scrofarum, Balantioides coli, and Blastocystis ST5, as well as HEV genotype 3, were detected. This highlights the need for enhanced surveillance and prevention measures targeting wild boar to prevent the spread of these pathogens to humans.
Article
Microbiology
Jenny G. Maloney, Aleksey Molokin, Raimundo Segui, Pablo Maravilla, Fernando Martinez-Hernandez, Guiehdani Villalobos, Anastasios D. Tsaousis, Eleni Gentekaki, Carla Munoz-Antoli, Debora R. Klisiowicz, Camila Y. Oishi, Rafael Toledo, J. Guillermo Esteban, Pamela C. Koster, Aida de Lucio, Alejandro Dashti, Begona Bailo, Rafael Calero-Bernal, David Gonzalez-Barrio, David Carmena, Monica Santin
Summary: Three recent studies found four novel sequences in mammalian hosts infected with Blastocystis, with B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. This study used Nanopore MinION sequencing to obtain full-length reference sequences for each of the new subtypes, and confirmed their validity through phylogenetic analysis and pairwise distance comparisons. The proposed subtype designations for the novel sequences in this study are ST35-ST38, which will aid in future accurate sequence descriptions and studies of Blastocystis epidemiology and subtype diversity.
Article
Infectious Diseases
Mercedes Chozas, Alejandro Dashti, Laura Prieto-Perez, Ramon Perez-Tanoira, Elena Cobo, Begona Bailo, Marta del Palacio, Carolina Hernandez-Castro, David Gonzalez-Barrio, David Carmena, Pamela C. Koster
Summary: Microsporidia are common parasites in immunocompromised individuals, such as HIV-infected patients. In this study, we found two microsporidian pathogens, Enterocytozoon bieneusi and Encephalitozoon intestinalis, in HIV-positive patients in Madrid. In addition, we also identified various protist infections, including Blastocystis spp., Giardia duodenalis, and Cryptosporidium spp. These findings highlight the need to consider microsporidia and protist infections in the diagnosis of diarrhea in HIV-positive patients.
Article
Veterinary Sciences
Ehab Kotb Elmahallawy, Pamela C. Koster, Alejandro Dashti, Samia Qasem Alghamdi, Amira Saleh, Ahmed Gareh, Barakat M. Alrashdi, Carolina Hernandez-Castro, Begona Bailo, Maha S. Lokman, Eman A. A. Hassanen, David Gonzalez-Barrio, David Carmena
Summary: This study investigated the occurrence of microeukaryotic gut parasites in dromedary camels in Egypt. The most common parasites found were Giardia duodenalis and Cryptosporidium spp. The presence of zoonotic Cryptosporidium parvum suggests potential health risks to livestock and humans.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Infectious Diseases
Muyideen K. K. Tijani, Pamela C. C. Koster, Isabel Guadano-Procesi, Imo S. S. George, Elizabeth Abodunrin, Adedamola Adeola, Alejandro Dashti, Begona Bailo, David Gonzalez-Barrio, David Carmena
Summary: This study in Ibadan, Nigeria, examines the occurrence and molecular diversity of G. duodenalis and other intestinal parasites in apparently healthy children. G. duodenalis was found to be the most prevalent parasite, followed by Entamoeba spp., Ascaris lumbricoides, and Taenia sp. Most of the G. duodenalis positive samples were confirmed using qPCR and genotyped as assemblage B, indicating human transmission.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Pediatrics
Carolina Hernandez-Castro, Alejandro Dashti, Amoolya Vusirikala, Sooria Balasegaram, Pamela Carolina Koster, Begona Bailo, Elena Imana, Andrea Lopez, Maria Teresa Llorente, David Gonzalez-Barrio, Sergio Sanchez, David Carmena
Summary: Giardia duodenalis and Blastocystis sp. were commonly found in toddlers attending day-care centres in Central Spain. This study identified the genetic diversity and temporal dynamics of these two eukaryotic parasites, and found that they can persist in some children for a long period of time.
EUROPEAN JOURNAL OF PEDIATRICS
(2023)