Article
Materials Science, Biomaterials
Li Zhang, Xueliang Xiu, Zhipeng Li, Rui Su, Xuemei Li, Shichao Ma, Fengsen Ma
Summary: Porous microneedles (pMNs) are a novel transdermal drug delivery modality that can carry drugs and vaccines and exert a synergistic effect through the release of drugs and adjuvants in the skin. In this study, biocompatible pMNs with good mechanical properties and high porosity were successfully prepared. The coated pMNs induced an efficient immune response in rats. This research suggests that pMNs have a promising prospect for future applications in preventing global epidemics like influenza.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Immunology
Eve Victoria Singleton, Chloe Jayne Gates, Shannon Christa David, Timothy Raymond Hirst, Justin Bryan Davies, Mohammed Alsharifi
Summary: In this study, the immunogenicity of gamma-irradiated influenza vaccine under different temperature conditions was compared, and it was found that regardless of temperature, the structural integrity and vaccine efficacy were well maintained. Interestingly, using higher temperature and lower radiation dose seemed to induce stronger immune responses. This suggests that simplifying irradiation protocols to manufacture effective irradiated vaccines may be possible.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Yonghong Liu, Shirin Strohmeier, Irene Gonzalez-Dominguez, Jessica Tan, Viviana Simon, Florian Krammer, Adolfo Garcia-Sastre, Peter Palese, Weina Sun
Summary: By generating and sequentially vaccinating mice with inactivated influenza B viruses displaying mosaic HAs, researchers have successfully induced long-lasting and cross-protective antibody responses. These findings represent a significant step towards the development of a universal influenza B virus vaccine.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Constantina Boikos, Lauren Fischer, Dan O'Brien, Joe Vasey, Gregg C. Sylvester, James A. Mansi
Summary: This study estimated the relative vaccine effectiveness (rVE) of cell-propagated inactivated quadrivalent influenza vaccine (ccIIV4) versus egg-derived inactivated quadrivalent influenza vaccine (eIIV4) in preventing influenza-related medical encounters in the 2018-2019 US season. The results showed a statistically significantly greater reduction in influenza-related medical encounters in individuals vaccinated with ccIIV4 compared to eIIV4, supporting ccIIV4 as a potentially more effective public health measure against influenza.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Richard I. Walker, A. Louis Bourgeois
Summary: Oral immunization is an effective strategy against mucosal enteric pathogens. Inactivated whole bacterial cells can also induce protective immunity. Developing a safe and simple delivery format with controlled immunogenic amounts of complex antigens is essential. Enhancing the efficacy of whole cell vaccines can be achieved through genetic engineering and the use of mucosal adjuvants. The development of ETVAX, a vaccine candidate against enterotoxigenic Escherichia coli (ETEC), has shown promising results.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Mahmoud Ameri, Yi Ao, Hayley Lewis
Summary: The high concentration trivalent influenza split vaccine was coated on transdermal microneedles. The vaccine formulation process successfully converted low HA concentration monovalent vaccine bulks into a viscous emulsion suitable for coating. The dry-coated trivalent vaccine delivered by transdermal microneedle system elicited both primary and recall antibody responses against all three antigen strains, demonstrating an attractive alternative for influenza vaccine delivery.
Article
Biochemistry & Molecular Biology
Keegan Braz Gomes, Sharon Vijayanand, Priyal Bagwe, Ipshita Menon, Akanksha Kale, Smital Patil, Sang-Moo Kang, Mohammad N. Uddin, Martin J. D'Souza
Summary: This study developed an influenza vaccine using polymeric nanoparticles delivered through dissolving microneedles. The vaccine particles effectively stimulated antigen-presenting cells, resulting in enhanced autophagy, nitric oxide, and antigen presentation. In mice, the vaccine induced the production of M2e-specific antibodies and significant expression of CD4(+) and CD8(+) populations. The study demonstrated that polymeric particulates delivered via fast-dissolving microneedles significantly enhanced the immunogenicity of a conserved influenza antigen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Chimuka Handabile, Toshiki Sekiya, Naoki Nomura, Marumi Ohno, Tomomi Kawakita, Masashi Shingai, Hiroshi Kida
Summary: Despite the use of vaccines, seasonal influenza remains a risk to public health. A study compared the immunological potency of a inactivated whole virus particle vaccine (WPV) and a split vaccine (SV) and found that WPV induced higher levels of antibodies against heterologous influenza viruses, providing better protection. Specifically, WPV induced neuraminidase-inhibition (NI) antibodies that recognized NA of divergent strains, contributing to its effectiveness in protecting against infection from heterologous influenza viruses.
Article
Immunology
Seo-jeong Park, Yong-Myung Kang, Hyun-Kyu Cho, Do-Young Kim, Sungyeop Kim, Youchan Bae, Jongho Kim, Gyeongyeob Kim, Youn-Jeong Lee, Hyun-Mi Kang
Summary: Two whole inactivated vaccines were developed against different lineages of the H9N2 virus and evaluated for their immunogenicity and protective efficacy in mice. Both vaccines provided 100% protection in homologous challenge, but only showed partial protection in heterologous challenge.
Article
Engineering, Marine
Po-Tsang Lee, Jing Huang, Fan-Hua Nan
Summary: This study reports the first case of lethal vibriosis infection in cultured O. punctatus and demonstrates the high level of protection provided by a vaccine against this infection.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Microbiology
Yassmin Moatasim, Ahmed Kandeil, Ahmed Mostafa, Omnia Kutkat, Mohamed El Sayes, Ahmed N. El Taweel, Maha AlKhazindar, Elsayed T. AbdElSalam, Rabeh El-Shesheny, Ghazi Kayali, Mohamed A. Ali
Summary: Research has shown that besides expressing the HA of H5N8, PR8 vaccine strains expressing other internal proteins from the H5N8 strain are unable to efficiently elicit virus-neutralizing antibodies, but can provide some protection to infected chickens six days post infection, possibly related to cell-based immunity.
Article
Biology
Zhegang Zhang, Zheng Jiang, Tao Deng, Jiayou Zhang, Bo Liu, Jing Liu, Ran Qiu, Qingmei Zhang, Xuedan Li, Xuanxuan Nian, Yue Hong, Fang Li, Feixia Peng, Wei Zhao, Zhiwu Xia, Shihe Huang, Shuyan Liang, Jinhua Chen, Changgui Li, Xiaoming Yang
Summary: In the development of influenza vaccines, MDCK cells offer advantages such as large-scale production and independence from eggs. This study cultured H5N1 virus in serum-free MDCK cell suspension and manufactured vaccines from the harvested virus. The vaccine's effectiveness, immunological responses, and protective effects were evaluated, showing that the vaccine induced strong immune responses and provided protection against clinical symptoms and virus titer in mice after challenge.
OPEN LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Meito Shibuya, Shigeyuki Tamiya, Atsushi Kawai, Yasuo Yoshioka
Summary: The study examines the use of different vaccination routes for priming and boosting to achieve protection in both the upper and lower respiratory tracts. Results show that intranasal priming followed by subcutaneous boosting induces both virus-specific IgG in plasma and IgA in nasal washes, providing protection against virus challenge in both regions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Immunology
Flora Carnet, Romain Paillot, Christine Fortier, Erika S. S. Hue, Laurie Briot, Frederic de Geoffroy, Pierre-Olivier Vidalain, Stephane Pronost
Summary: By stimulating the immune system with iPPVO after vaccination, the effectiveness of equine influenza virus (EIV) vaccines can be improved, leading to higher levels of antibodies produced. The study found that iPPVO could enhance the immune response and increase protective antibodies in horses receiving the EIV vaccine boost.
Article
Immunology
Masashi Shingai, Naoki Nomura, Toshiki Sekiya, Marumi Ohno, Daisuke Fujikura, Chimuka Handabile, Ryosuke Omori, Yuki Ohara, Tomohiro Nishimura, Masafumi Endo, Kazuhiko Kimachi, Ryotarou Mitsumata, Tomio Ikeda, Hiroki Kitayama, Hironori Hatanaka, Tomoyoshi Sobue, Fumihito Muro, Saori Suzuki, Cong Thanh Nguyen, Hirohito Ishigaki, Misako Nakayama, Yuya Mori, Yasushi Itoh, Marios Koutsakos, Brendon Y. Chua, Lorena E. Brown, David C. Jackson, Katherine Kedzierska, Kazumasa Ogasawara, Yoichiro Kino, Hiroshi Kida
Summary: The study showed that whole virus particle vaccines (WPVs) are more effective in priming immune responses and inducing innate immune responses compared to split vaccines (SVs), making them a potential alternative for seasonal influenza vaccines, especially for children.
Article
Instruments & Instrumentation
Erkan Azizoglu, Ozgen Ozer, Mark R. Prausnitz
Summary: The study developed dissolving microneedle patches for transdermal delivery of montelukast sodium, showing drug localization and selective loading can be achieved through solvent washing method, with demonstrated stability in storage and delivery efficiency.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Jie Tang, Richard Schutzman, Carlos A. Rodriguez, Joerg Lahann, Nair Rodriguez-Hornedo, Mark R. Prausnitz, Steven P. Schwendeman
Summary: This study aimed to develop an injectable poly(D,L-lactide) (PLA) microparticle for sustained release of contraceptive hormone, etonogestrel (ENG). The ENG-cs-MPs prepared by the coaxial electrospray method showed high drug loading, high encapsulation efficiency, and long-term stable release, suggesting their potential as a long-acting injectable contraceptive with improved pharmacokinetic profile.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Nanoscience & Nanotechnology
Wei Li, Jie Tang, Dennis Lee, Thomas R. Tice, Steven P. Schwendeman, Mark R. Prausnitz
Summary: Long-acting drug delivery formulations enable sustained and prolonged drug release, improving patient compliance. This review discusses FDA-approved long-acting drug delivery formulations, including different slow-release mechanisms and materials, and provides a comprehensive summary of their clinical applications and product information.
NATURE REVIEWS MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Rohan Murty, Abishek Sankaranarayanan, Isabella I. Bowland, Juan Mena-Lapaix, Mark R. Prausnitz
Summary: This study successfully localized antigen delivery to the epidermis using angled insertion of microneedles. Angled insertion significantly reduced microneedle insertion depth and increased epidermal localization of antigen delivery.
Article
Endocrinology & Metabolism
Kevin T. Nguyen, Nicole Y. Xu, Jennifer Y. Zhang, Trisha Shang, Ananda Basu, Richard M. Bergenstal, Kristin Castorino, Kong Y. Chen, David Kerr, Suneil K. Koliwad, Lori M. Laffel, Nestoras Mathioudakis, L. Kurt Midyett, Joshua D. Miller, James H. Nichols, Francisco J. Pasquel, Priya Prahalad, Mark R. Prausnitz, Jane Jeffrie Seley, Jennifer L. Sherr, Elias K. Spanakis, Guillermo E. Umpierrez, Amisha Wallia, David C. Klonoff
Summary: This article is the outcome of the Continuous Ketone Monitoring Consensus Panel, which consisted of 20 US-based experts. The panel reviewed medical literature on various topics related to continuous ketone monitoring and developed 30 conclusions unanimously. The report aims to support the development and application of this technology for the benefit of people with diabetes.
JOURNAL OF DIABETES SCIENCE AND TECHNOLOGY
(2022)
Article
Instruments & Instrumentation
Hagar Labouta, Robert Langer, Pieter R. Cullis, Olivia M. Merkel, Mark R. Prausnitz, Yasmine Gomaa, Sara S. Nogueira, Tushar Kumeria
Summary: The successful development of mRNA vaccines highlights the crucial role of drug delivery technologies in protecting billions of people from COVID-19. These vaccines, built upon years of scientific research in mRNA drug delivery, vaccines, and other biologicals, have proven to be safe and effective in preventing the disease.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Ophthalmology
Brandon G. Gerberich, Bailey G. Hannon, Dillon M. Brown, A. Thomas Read, Matthew D. Ritch, Elisa Schrader Echeverri, Lauren Nichols, Cahil Potnis, Sreesh Sridhar, Maya G. Toothman, Stephen A. Schwaner, Erin J. Winger, Hannah Huang, Gabby S. Gershon, Andrew J. Feola, Machelle T. Pardue, Mark R. Prausnitz, C. Ross Ethier
Summary: This study assessed the neuroprotective effects of scleral stiffening treatments in an experimental model of glaucoma. The results showed that neither global nor local scleral stiffening provided demonstrable neuroprotection in hypertensive eyes.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Brandon G. Gerberich, Amy J. Wood-Yang, Afsane Radmand, Lauren M. Nichols, Amir Hejri, Elisa Schrader Echeverri, Hannah G. Gersch, Mark R. Prausnitz
Summary: A computational model was developed to predict the crosslinking efficiency of visible/near infrared photosensitizers in the sclera for the treatment of myopia and glaucoma. The study validated photocrosslinking against riboflavin corneal crosslinking experimental studies and simulated the treatment using methylene blue administered by retrobulbar injection and irradiated with a transpupillary light beam. Optimal ranges for treatment parameters were determined and the sensitivity of crosslinking to various parameters was quantified, with oxygen concentration in the injection solution, scleral thickness, and injection volume being the most sensitive parameters.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Wei Li, Jonathan Yuxuan Chen, Richard N. Terry, Jie Tang, Andrey Romanyuk, Steven P. Schwendeman, Mark R. Prausnitz
Summary: There is a great need for a simple and long-acting contraception method. This study developed a core-shell microneedle patch that achieved zero-order release of a contraceptive hormone for 6 months, compared to a monolithic microneedle patch with a shorter release time. The core-shell design effectively controlled the burst release of the hormone.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Engineering, Biomedical
Andrey Romanyuk, Ruixue Wang, Alexander Marin, Benjamin M. M. Janus, Eric I. I. Felner, Dengning Xia, Yenny Goez-Gazi, Kendra J. J. Alfson, Abdul S. S. Yunus, Eric A. A. Toth, Gilad Ofek, Ricardo Carrion Jr, Mark R. R. Prausnitz, Thomas R. R. Fuerst, Alexander K. K. Andrianov
Summary: This study describes a novel MN technology that combines EBOV GP antigen with a polyphosphazene-based immunoadjuvant and vaccine delivery system. The prepared dissolvable EBOV GP MN patch vaccine showed superior antigenicity compared to a non-polyphosphazene polymer-based analog. Mice vaccinated with the polyphosphazene-based MN patches exhibited strong antibody responses against EBOV GP and complete protection against lethal challenge.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Youngeun Kim, Jae Hwan Jung, Andrew R. Tadros, Mark R. Prausnitz
Summary: Topical application of STAR particles with microneedle protrusions can create micropores on the skin, significantly increasing skin permeability even to water-soluble compounds and macromolecules. This study demonstrates the tolerability, acceptability, and reproducibility of STAR particles on human subjects at various pressures and repeated applications. The results show that one-time application of STAR particles at pressures between 40 and 80 kPa leads to skin microporation and erythema, with increased pressure directly correlating to these effects, and most subjects reported comfort with STAR particles. Repeated application for 10 consecutive days at 80 kPa showed similar skin microporation, erythema, and comfort, indicating the safety and reliability of STAR particles for enhancing cutaneous drug delivery.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Euna Oh, Seung Joon Choi, Sumin Han, Kyu Hyoung Lee, Hyo-Jick Choi
Summary: This study aims to improve the efficiency of alcohol-based disinfectants by adding a physical pathogen inactivation mechanism through salt recrystallization, preventing the emergence of alcohol tolerance. The experiment showed that additional salt crystallization facilitated stronger biocidal effects compared to alcohol-only formulations, regardless of the types of solid surfaces and pathogens.
Review
Pharmacology & Pharmacy
Sumin Han, Panjae Lee, Hyo-Jick Choi
Summary: Non-invasive vaccines have gained attention due to the limitations of conventional invasive vaccines. Various approaches for formulating and administrating non-invasive vaccines have emerged, but each faces challenges associated with vaccine bioavailability, toxicity, and other issues. Researchers have developed novel supplementary materials and delivery systems to overcome these limitations and this review article aims to provide up-to-date information and guide future research and development efforts in vaccine formulation for non-invasive vaccines.
Article
Food Science & Technology
Manika Chopra, Surjith Kumar Kumaran, Euna Oh, Seok Chan Park, Seong-O Choi, Dominic Sauvageau, Hyo-Jick Choi
Summary: This study developed a microfabricated probiotic formulation that can protect the activity of probiotics in digestive conditions and environmental storage. The technology enhances the efficacy of probiotics and reduces the required amount and costs.
ACS FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Sumin Han, Euna Oh, Erin Keltie, Jong Sung Kim, Hyo-Jick Choi
Summary: Developing highly effective and low-cost methods to protect against direct transmission of respiratory diseases is a major challenge during pandemic outbreaks. Current filtration in respirators and masks raises safety concerns. Reusability of masks has gained attention during the COVID-19 pandemic. Our group has developed antimicrobial coating technologies for respiratory devices and focuses on salt-coated polypropylene fibers.
ACCOUNTS OF MATERIALS RESEARCH
(2022)