High-Throughput Ligand Discovery Reveals a Sitewise Gradient of Diversity in Broadly Evolved Hydrophilic Fibronectin Domains

Library preparation methods for next-generation sequencing: Tone down the bias

(2014) Erwin L. van Dijk et al.   EXPERIMENTAL CELL RESEARCH

Tracking Molecular Recognition at the Atomic Level with a New Protein Scaffold Based on the OB-Fold

(2014) John D. Steemson et al.   PLoS One

Potent and Specific Inhibition of Glycosidases by Small Artificial Binding Proteins (Affitins)

(2014) Agustín Correa et al.   PLoS One

Anti-Idiotypic Monobodies Derived from a Fibronectin Scaffold

(2013) Mark A. Sullivan et al.   BIOCHEMISTRY

What Makes a Protein Fold Amenable to Functional Innovation? Fold Polarity and Stability Trade-offs

(2013) Eynat Dellus-Gur et al.   JOURNAL OF MOLECULAR BIOLOGY

Tolerance of the archaeal Sac7d scaffold protein to alternative library designs: characterization of anti-immunoglobulin G Affitins

(2013) G. Behar et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Protein-protein interactions: General trends in the relationship between binding affinity and interfacial buried surface area

(2013) Jieming Chen et al.   PROTEIN SCIENCE

Design, construction, and characterization of a second-generation DARPin library with reduced hydrophobicity

(2013) Markus A. Seeger et al.   PROTEIN SCIENCE

Protein folding and de novo protein design for biotechnological applications

(2013) George A. Khoury et al.   TRENDS IN BIOTECHNOLOGY

Length and GC-biases during sequencing library amplification: A comparison of various polymerase-buffer systems with ancient and modern DNA sequencing libraries

(2012) Jesse Dabney et al.   BIOTECHNIQUES

PANDAseq: paired-end assembler for illumina sequences

(2012) Andre P Masella et al.   BMC BIOINFORMATICS

Construction of a Stability Landscape of the CH3 Domain of Human IgG1 by Combining Directed Evolution with High Throughput Sequencing

(2012) Michael W. Traxlmayr et al.   JOURNAL OF MOLECULAR BIOLOGY

Deep Sequencing of Systematic Combinatorial Libraries Reveals β-Lactamase Sequence Constraints at High Resolution

(2012) Zhifeng Deng et al.   JOURNAL OF MOLECULAR BIOLOGY

Combinatorial Design of an Anticalin Directed against the Extra-Domain B for the Specific Targeting of Oncofetal Fibronectin

(2012) Michaela Gebauer et al.   JOURNAL OF MOLECULAR BIOLOGY

Generation, characterization and structural data of chymase binding proteins based on the human Fyn kinase SH3 domain

(2012) Daniel Schlatter et al.   mAbs

Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing

(2012) Timothy A Whitehead et al.   NATURE BIOTECHNOLOGY

Designed hydrophilic and charge mutations of the fibronectin domain: towards tailored protein biodistribution

(2012) B. J. Hackel et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Protease-Resistant Peptide Ligands from a Knottin Scaffold Library

(2011) Jennifer A. Getz et al.   ACS Chemical Biology

Highly Stable Binding Proteins Derived from the Hyperthermophilic Sso7d Scaffold

(2011) Nimish Gera et al.   JOURNAL OF MOLECULAR BIOLOGY

HuCAL PLATINUM, a Synthetic Fab Library Optimized for Sequence Diversity and Superior Performance in Mammalian Expression Systems

(2011) Josef Prassler et al.   JOURNAL OF MOLECULAR BIOLOGY

Synthetic Antibodies Designed on Natural Sequence Landscapes

(2011) Wenwu Zhai et al.   JOURNAL OF MOLECULAR BIOLOGY

Teaching an Old Scaffold New Tricks: Monobodies Constructed Using Alternative Surfaces of the FN3 Scaffold

(2011) Akiko Koide et al.   JOURNAL OF MOLECULAR BIOLOGY

A De Novo Protein Binding Pair By Computational Design and Directed Evolution

(2011) John Karanicolas et al.   MOLECULAR CELL

Isoform-specific monobody inhibitors of small ubiquitin-related modifiers engineered using structure-guided library design

(2011) R. N. Gilbreth et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational Design of Proteins Targeting the Conserved Stem Region of Influenza Hemagglutinin

(2011) S. J. Fleishman et al.   SCIENCE

Stability and CDR Composition Biases Enrich Binder Functionality Landscapes

(2010) Benjamin J. Hackel et al.   JOURNAL OF MOLECULAR BIOLOGY

The functional capacity of the natural amino acids for molecular recognition

(2010) Sara Birtalan et al.   Molecular BioSystems

Coevolution of PDZ domain–ligand interactions analyzed by high-throughput phage display and deep sequencing

(2010) Andreas Ernst et al.   Molecular BioSystems

Ribosome Display Selection of a Murine IgG1 Fab Binding Affibody Molecule Allowing Species Selective Recovery Of Monoclonal Antibodies

(2010) Sebastian Grimm et al.   MOLECULAR BIOTECHNOLOGY

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain

(2010) John Wojcik et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

By-passing in vitro screening—next generation sequencing technologies applied to antibody display and in silico candidate selection

(2010) U. Ravn et al.   NUCLEIC ACIDS RESEARCH

An improved yeast transformation method for the generation of very large human antibody libraries

(2010) Lorenzo Benatuil et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Adnectins: engineered target-binding protein therapeutics

(2010) D. Lipovsek   PROTEIN ENGINEERING DESIGN & SELECTION

The Importance of Being Tyrosine: Lessons in Molecular Recognition from Minimalist Synthetic Binding Proteins

(2009) Shohei Koide et al.   ACS Chemical Biology

Highly avid magnetic bead capture: An efficient selection method for de novo protein engineering utilizing yeast surface display

(2009) Margaret Ackerman et al.   BIOTECHNOLOGY PROGRESS

Stability effects of mutations and protein evolvability

(2009) Nobuhiko Tokuriki et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Accelerating phage-display library selection by reversible and site-specific biotinylation

(2009) A. Koide et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The Intrinsic Contributions of Tyrosine, Serine, Glycine and Arginine to the Affinity and Specificity of Antibodies

(2008) Sara Birtalan et al.   JOURNAL OF MOLECULAR BIOLOGY

Picomolar Affinity Fibronectin Domains Engineered Utilizing Loop Length Diversity, Recursive Mutagenesis, and Loop Shuffling

(2008) Benjamin J. Hackel et al.   JOURNAL OF MOLECULAR BIOLOGY