☆ 4.6 Article

Genome Wide Association Mapping for Arabinoxylan Content in a Collection of Tetraploid Wheats

PLOS ONE (2015)

Journal

PLOS ONE

Volume 10, Issue 7, Pages -

Publisher

PUBLIC LIBRARY SCIENCE

DOI: 10.1371/journal.pone.0132787

Keywords

Funding

Ministero dell'Istruzione, dell'Universita' e della Ricerca ['PON-01_01145]
BBSRC [BB/J014869/1]
Australian Research Council
South Australia-Puglia Awards scheme of South Australia
BBSRC [BB/L026317/1, BB/J014869/1] Funding Source: UKRI
Biotechnology and Biological Sciences Research Council [BB/L026317/1, BB/J014869/1] Funding Source: researchfish

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Abstract

Background Arabinoxylans (AXs) are major components of plant cell walls in bread wheat and are important in bread-making and starch extraction. Furthermore, arabinoxylans are components of soluble dietary fibre that has potential health-promoting effects in human nutrition. Despite their high value for human health, few studies have been carried out on the genetics of AX content in durum wheat. Results The genetic variability of AX content was investigated in a set of 104 tetraploid wheat genotypes and regions attributable to AX content were identified through a genome wide association study (GWAS). The amount of arabinoxylan, expressed as percentage (w/w) of the dry weight of the kernel, ranged from 1.8% to 5.5% with a mean value of 4.0%. The GWAS revealed a total of 37 significant marker-trait associations (MTA), identifying 19 quantitative trait loci (QTL) associated with AX content. The highest number of MTAs was identified on chromosome 5A (seven), where three QTL regions were associated with AX content, while the lowest number of MTAs was detected on chromosomes 2B and 4B, where only one MTA identified a single locus. Conservation of synteny between SNP marker sequences and the annotated genes and proteins in Brachypodium distachyon, Oryza sativa and Sorghum bicolor allowed the identification of nine QTL coincident with candidate genes. These included a glycosyl hydrolase GH35, which encodes Gal7 and a glucosyltransferase GT31 on chromosome 1A; a cluster of GT1 genes on chromosome 2B that includes TaUGT1 and cisZog1; a glycosyl hydrolase that encodes a CelC gene on chromosome 3A; Ugt12887 and TaUGT1 genes on chromosome 5A; a (1,3)-beta-D-glucan synthase (Gsl12 gene) and a glucosyl hydrolase (Cel8 gene) on chromosome 7A. Conclusions This study identifies significant MTAs for the AX content in the grain of tetraploid wheat genotypes. We propose that these may be used for molecular breeding of durum wheat varieties with higher soluble fibre content.

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Genome-Wide Association Study of Arabinoxylan Content from a 562 Hexaploid Wheat Collection

Myoung Hui Lee, Jinhee Park, Kyeong-Hoon Kim, Kyeong-Min Kim, Chon-Sik Kang, Go Eun Lee, Jun Yong Choi, Jiyoung Shon, Jong-Min Ko, Changhyun Choi

Summary: This study identified 8 candidate genes that are highly correlated with high arabinoxylan (AX) levels in wheat. These genes include those encoding F-box domain-containing proteins, disease resistance protein RPM1, and bZIP transcription factor 29. The adenine allele in these genes, particularly in East Asian wheat genotypes, showed a high correlation with AX levels, indicating their potential as selectable markers for high AX content.

PLANTS-BASEL (2023)