Article
Biochemistry & Molecular Biology
Sonia Eligini, Marco Munno, Daphne Atlas, Cristina Banfi
Summary: In this study, the effects of small-molecular-weight redox molecules on collagen-induced platelet aggregation were tested. N-acetylcysteine amide (AD4/NACA), a thiol antioxidant with improved lipophilicity and bioavailability compared to N-acetylcysteine (NAC), and various thioredoxin-mimetic (TXM) peptides were used. All compounds significantly inhibited platelet aggregation induced by collagen, with TXM peptides and AD4 being more effective than NAC. These findings suggest that these small-molecular-weight redox peptides might be useful for preventing or treating oxidative stress conditions associated with platelet activation.
Article
Neurosciences
Kristina Xiao Liang, Guro Helen Vatne, Cecilie Katrin Kristiansen, Oleksandr Ievglevskyi, Elena Kondratskaya, Joel C. Glover, Anbin Chen, Gareth John Sullivan, Laurence A. Bindoff
Summary: The study successfully generated functional dopaminergic neurons using human induced pluripotent stem cells, which replicated the molecular and biochemical changes found in post-mortem brain samples of POLG patients. POLG-DA neurons exhibited mitochondrial dysfunction, loss of mtDNA and complex I compared to disease-free DA neurons, and NACA showed promising therapeutic effects on this deficit.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ahmed Fareed Alkandari, Sampath Madhyastha, Muddanna S. Rao
Summary: Oxidative stress and depletion of glutathione play a crucial role in the onset and progression of Alzheimer's disease. N-Acetylcysteine amide (NACA), a form of glutathione precursor, has extended bioavailability and shows neuroprotective effects in AD animal models. This study investigates the potential neuroprotective effects of NACA against AD-like pathology induced by Aβ1-42 peptide in rats.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Peng Dong, Qingshan Shi, Ruqun Peng, Yingzi Yuan, Xiaobao Xie
Summary: By modifying chitosan oligosaccharide (COS) with the Eschweiler-Clarke reaction, a derivative called DMCOS was synthesized. Analysis revealed that DMCOS had converted primary amines into tertiary amines after methylation. DMCOS showed less thermal stability than COS, but exhibited stronger antimicrobial activity against bacterial and yeast strains, while also displaying lower antioxidant activity and causing mitochondrial damage in C. albicans cells.
CARBOHYDRATE POLYMERS
(2023)
Article
Oncology
Elena Obrador, Rosario Salvador-Palmer, Rafael Lopez-Blanch, Maria Oriol-Caballo, Paz Moreno-Murciano, Jose M. Estrela
Summary: Malignant melanoma, a cancer derived from melanocytes, can develop on skin, mucous membranes, and other locations. N-acetylcysteine (NAC), an antioxidant drug, may play a role in preventing cancer, but high doses could potentially cause metastatic spread of melanoma. Antioxidant properties of NAC and other promoters of glutathione synthesis should be used cautiously in cancer patients to avoid promoting metastatic growth.
Article
Behavioral Sciences
Durmus Ali Aslanlar, Emin Fatih Visneci, Mehmet Oz, K. Esra Nurullahoglu Atalik
Summary: Mood disorders caused by chemotherapy have become more important as cancer patients' survival increases. This study used methotrexate to induce mood disorders in rats and found that treatment with the antioxidant N-acetylcysteine (NAC) can alleviate anxiety and depression-like behaviors, increase antioxidant capacity, reduce oxidative stress and inflammatory response, and regulate brain chemistry. The findings suggest that NAC treatment could be an effective strategy in revising the treatment for individuals suffering from chemotherapy-induced mood disorders.
BEHAVIOURAL BRAIN RESEARCH
(2024)
Article
Multidisciplinary Sciences
Jianying Zhang, Feng Li, Tyler Augi, Kelly M. Williamson, Kentaro Onishi, MaCalus Hogan, Matthew D. Neal, James H-C Wang
Summary: Platelet-rich plasma (PRP) containing PLT HMGB1 plays a crucial role in tendon wound healing by reducing inflammation, increasing local HMGB1 levels, and recruiting stem cells to the injured area. The efficacy of PRP treatment for tendon injuries in clinics may be dependent on the presence of PLT HMGB1 in PRP preparations.
Article
Environmental Sciences
Li Cao, Jie Zhao, Jingru Xu, Lei Zhu, Sajid Ur Rahman, Shibin Feng, Yu Li, Jinjie Wu, Xichun Wang
Summary: The study demonstrates that N-acetylcysteine (NAC) can alleviate the damage of Sertoli cells induced by Zearalenone (ZEA) and Deoxynivalenol (DON) by reducing oxidative damage and apoptosis.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Medicine, Research & Experimental
Shyng-Shiou F. Yuan, Yun-Ming Wang, Leong-Perng Chan, Amos C. Hung, Hieu D. H. Nguyen, Yuk-Kwan Chen, Stephen Chu-Sung Hu, Steven Lo, Yen-Yun Wang
Summary: IL-1RA promotes the development of oral squamous cell carcinoma (OSCC) by regulating mitochondrial metabolism-mediated EGFR/JNK activation and SOX2 expression. Inhibition of this mitochondrial metabolic pathway may be a potential therapeutic strategy in OSCC.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Food Science & Technology
Sirao Hai, Jie Zhao, Chuangjiang Chen, Chenlong Wang, Li Ma, Sajid Ur Rahman, Chang Zhao, Shibin Feng, Jinjie Wu, Xichun Wang
Summary: This study reveals the mechanism of Zearalenone-induced apoptosis in porcine endometrial stromal cells, involving the activation of the JNK signaling pathway and mitochondrial division. These findings provide a conceptual foundation for the prevention and control of Zearalenone.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Cell Biology
Jing-Jie Xiao, Qing Liu, Ying Li, Fang-Fang Peng, Shan Wang, Zhihan Zhang, Hui Liu, Hong Yu, Shengxiang Tao, Bai-Fang Zhang
Summary: The study found that RCAN1 is upregulated in I/R and cisplatin-induced AKI models as well as in HR models. RCAN1 deficiency reduces kidney damage, mitochondrial dysfunction, and cell apoptosis, while RCAN1 overexpression leads to the opposite phenotypes. Further mechanistic exploration reveals that RCAN1 activates the JNK/Mff signaling pathway, promoting mitochondrial fission in renal TECs.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Muhammad Umar Ijaz, Sabahat Shahzadi, Asma Ashraf, Shahid Mahboob, K. A. Al-Ghanim, Mian N. Riaz, F. Al-Misned, Salma Sultana
Summary: Baicalin protects against MTX-induced mitochondrial damage in rat testicular tissues, reducing oxidative stress and preserving mitochondrial function.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sonia Eligini, Benedetta Porro, Giancarlo Aldini, Susanna Colli, Cristina Banfi
Summary: N-acetylcysteine (NAC) inhibits platelet aggregation by restoring mercaptoalbumin (HMA) and reducing levels of oxidized albumin, which are associated with diseases related to oxidative stress. This study also shows that cysteine can have similar effects as NAC in inhibiting platelet aggregation by restoring HMA.
Article
Biochemistry & Molecular Biology
Yelei Cen, Guohua Lou, Jinjin Qi, Minwei Li, Min Zheng, Yanning Liu
Summary: This study aimed to investigate the beneficial effects and potential therapeutic mechanisms of adipose-derived mesenchymal stem cell (AMSC) therapy on acetaminophen-induced liver injury (AILI). The results showed that AMSC therapy effectively ameliorated AILI by inhibiting the JNK-ATM mitochondrial retrograde pathway, improving APAP-induced mitochondrial dysfunction and liver injury.
Article
Cell Biology
Winston Tse-Hou Kwok, Haejin Angela Kwak, Ana Cristina Andreazza
Summary: Mitochondrial Complex I dysfunction and oxidative stress are implicated in various diseases, and understanding cellular responses and adaptations to these dysfunctions is crucial for the development of mitochondria-targeted therapeutic strategies. This study investigated the effects of low doses of rotenone, an inhibitor of mitochondrial Complex I, on THP-1 cells, and evaluated the potential of N-acetylcysteine (NAC) in mitigating rotenone-induced mitochondrial dysfunction. The results demonstrate that rotenone exposure increased mitochondrial superoxide, cell-free mitochondrial DNA, and protein levels of the NDUFS7 subunit in THP-1 cells. NAC pre-treatment attenuated the increase of cell-free mitochondrial DNA and NDUFS7 protein levels, but not mitochondrial superoxide. Additionally, rotenone exposure induced glutathionylation of the NDUFV1 subunit. In conclusion, NAC may have a protective effect on Complex I and maintain mitochondrial function in THP-1 cells.
Article
Cell Biology
Feifei Cheng, Xuejuan Wang, Yi-Shiou Chiou, Chuyu He, Hui Guo, Yan Qin Tan, Basappa Basappa, Tao Zhu, Vijay Pandey, Peter E. Lobie
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a deadly cancer with poor prognosis. Elevated expression of trefoil factor 3 (TFF3) in PDAC is associated with worse patient survival. TFF3 promotes PDAC progression by activating the WNT pathway. Combination therapy targeting TFF3 and WNT signaling attenuates PDAC xenograft growth and enhances the efficacy of gemcitabine.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Barbara Guerra, Thomas K. Doktor, Sabrina B. Frederiksen, Kumar Somyajit, Brage S. Andresen
Summary: This study identifies the protein kinase CK2 alpha as critical for maintaining replisome integrity and optimizing ATR-CHK1 signaling activation upon replication stress.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Xi Zhang, Peng Huang, Liqiong Wang, Shu Chen, Basappa Basappa, Tao Zhu, Peter E. Lobie, Vijay Pandey
Summary: Loss of PTEN in endometrial carcinoma cells increases phosphorylation of BADSer99 (BADS99). Inhibition of BADS99 phosphorylation enhances apoptosis and reduces cell viability in PTEN-deficient EC cells. Combined inhibition of BADS99 phosphorylation and PARP improves the prognosis of recurrent EC patients by promoting apoptosis and DNA damage.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
S. S. Mahalingam, S. Jayaraman, N. Bhaskaran, E. Schneider, F. Faddoul, A. Paes da Silva, M. M. Lederman, R. Asaad, K. Adkins-Travis, L. P. Shriver, P. Pandiyan
Summary: By comparing protein expression, transcriptome, and salivary metabolome profiles of uninfected and HIV+ individuals, it was found that HIV infection leads to perturbations in polyamine metabolism in the oral mucosa. Further studies using an in vitro human tonsil organoid infection model revealed that HIV infection of T cells enhances polyamine synthesis, which is dependent on the activities of caspase-1, IL-1 beta, and ornithine decarboxylase-1. Blockade of caspase-1 and polyamine synthesis intermediates reversed the dysfunctional T cell phenotype, indicating the direct role of the polyamine pathway in altering T cell functions during HIV-1 infection.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Basappa Basappa, Lisha K. Poonacha, Zhang Xi, Divakar Vishwanath, Ji-Rui Yang, Omantheswara Nagaraja, Ananda Swamynayaka, Mahendra Madegowda, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Doddahosuru Mahadevappa Gurudatt, Vijay Pandey, Nanjundaswamy Shivananju, Kwang Seok Ahn, Gautam Sethi, Peter E. Lobie, Priya Babu Shubha
Summary: Small molecules are used to inhibit CDK enzymes in cancer treatment. Pyranopyrazoles were identified as CDK inhibitors synthesized by nano-zirconium dioxide via multicomponent reaction. The bioactive pyranopyrazoles target CDK1 in human breast cancer cells.
Article
Biotechnology & Applied Microbiology
Akshay Ravish, Rashmi Shivakumar, Zhang Xi, Min Hee Yang, Ji-Rui Yang, Ananda Swamynayaka, Omantheswara Nagaraja, Mahendra Madegowda, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Vijay Pandey, Gautam Sethi, Kwang Seok Ahn, Peter E. E. Lobie, Basappa Basappa
Summary: This study explored the design of imidazopyridine-tethered pyrazolines as a de novo drug strategy for inhibiting STAT3 phosphorylation in human BC cells. Compound 3f with 2,3-dichlorophenyl substitution was recognized among the tested series as a lead structure that inhibited the viability of MCF-7 cells. It effectively inhibited STAT3 phosphorylation in MCF-7 and T47D cells, indicating that these structures may be an alternative synthon to target STAT3 signaling in BC.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Na Young Kim, Divakar Vishwanath, Zhang Xi, Omantheswara Nagaraja, Ananda Swamynayaka, Keshav Kumar Harish, Shreeja Basappa, Mahendra Madegowda, Vijay Pandey, Gautam Sethi, Peter E. Lobie, Kwang Seok Ahn, Basappa Basappa
Summary: A JNK-targeting compound has been developed for HER2-positive breast cancer, which selectively inhibits cell proliferation and induces DNA damage and apoptosis. Further studies showed that the compound could interact with JNK and enhance its phosphorylation. These findings contribute to the development of new JNK-targeting compounds for HER2-positive breast cancer.
Article
Biochemistry & Molecular Biology
Basappa Basappa, Young Yun Jung, Akshay Ravish, Zhang Xi, Ananda Swamynayaka, Mahendra Madegowda, Vijay Pandey, Peter E. Lobie, Gautam Sethi, Kwang Seok Ahn
Summary: Nuclear factor kappa beta (NF-kappa B) plays a crucial role in cancer cell proliferation and inhibiting its activity can be beneficial in cancer therapy. In this study, a novel compound, 2-(((1-(3,4-dichlorophenyl)-1H-1,2,3-triazol-4-yl)methyl)thio)-5-(4-methoxybenzyl)-1,3,4-oxadiazole (4c), was synthesized via click chemistry. Compound 4c showed reduced viability of MCF-7 cells and caused loss of cell viability in chronic myelogenous leukemia (CML) cells. It also inhibited the activation of NF-kappa B in CML cells and induced apoptosis. The compound targeted COX-2 and may serve as a potential drug candidate.
Article
Medicine, Research & Experimental
Vignesh Ramesh, Paradesi Naidu Gollavilli, Luisa Pinna, Mohammad Aarif Siddiqui, Adriana Martinez Turtos, Francesca Napoli, Yasmin Antonelli, Aldo Leal-Egana, Jesper Foged Havelund, Simon Toftholm Jakobsen, Elisa Le Boiteux, Marco Volante, Nils Joakim Faergeman, Ole N. Jensen, Rasmus Siersbaek, Kumar Somyajit, Paolo Ceppi
Summary: This study found an inverse association between short-chain fatty acids and EMT in non-small cell lung cancer patients. In vitro experiments showed that propionate treatment enhanced the epithelial transcriptional program and reduced the EMT phenotype in lung cancer cell lines. Animal experiments also confirmed that propionate can reduce lung cancer metastasis and lymph node spread. Further mechanistic investigation revealed that propionate treatment caused chromatin remodeling through p300-mediated histone acetylation.
EMBO MOLECULAR MEDICINE
(2023)
Article
Chemistry, Inorganic & Nuclear
Sabiha A. Shaikh, Satish S. Bhat, Vidyanand K. Revankar, S. Naveen, N. K. Mahesha, N. K. Loknath, Vijay Kumbar
Summary: Mononuclear later first-row transition metal complexes incorporating 1,8-naphthalimide based ligand were designed and their photophysical properties and antibacterial activities were studied. Ni(II) and Cu(II) complexes exhibited good activity against certain bacterial strains.
JOURNAL OF COORDINATION CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Divakar Vishwanath, Zhang Xi, Akshay Ravish, Arunkumar Mohan, Shreeja Basappa, Niranjan Pattehalli Krishnamurthy, Santosh L. Gaonkar, Vijay Pandey, Peter E. Lobie, Basappa Basappa
Summary: Based on our study, we identified two new compounds, 5h and 6l, that can inhibit the proliferation of human breast cancer cells and have the potential to be developed as new drugs for cancer treatment.
Article
Chemistry, Physical
Akshay Ravish, Tejaswini P. Siddappa, Zhang Xi, Divakar Vishwanath, Arunkumar Mohan, Shreeja Basappa, Niranjan Pattehalli Krishnamurthy, Peter E. Lobie, Vijay Pandey, Basappa Basappa
Summary: Researchers have synthesized novel drug-like compounds using electrochemical and conventional methods and demonstrated their ability to inhibit the growth of breast cancer cells. Further analysis revealed that these compounds can target VEGFR-2, thereby inhibiting angiogenesis signaling.
Article
Chemistry, Multidisciplinary
Divakar Vishwanath, Anita Shete-Aich, Manjunath. B. B. Honnegowda, Mahesh Padukudru Anand, Saravana Babu Chidambaram, Gajanan Sapkal, Basappa Basappa, Pragya. D. D. Yadav
Summary: The COVID-19 pandemic has severely impacted global health and challenged public health systems worldwide. Researchers have identified synthetic compounds that can inhibit the replication of SARS-CoV-2 without toxic effects on host cells. This study provides encouraging results for further preclinical research.
Article
Cell Biology
Melissa Leriche, Clara Bonnet, Jagannath Jana, Gita Chhetri, Sabrina Mennour, Sylvain Martineau, Vincent Pennaneach, Didier Busso, Xavier Veaute, Pascale Bertrand, Sarah Lambert, Kumar Somyajit, Patricia Uguen, Stephan Vagner
Summary: RNA-binding protein 53BP1 is found to directly interact with Okazaki fragments and its recruitment is affected by RNA enzyme processing. RNA primers contribute to the recruitment of 53BP1 at replication forks, and depletion of 53BP1 leads to the accumulation of S-phase poly(ADP-ribose).
Article
Chemistry, Multidisciplinary
Sabiha A. Shaikh, Satish S. Bhat, Vidyanand K. Revankar, S. Naveen, Karthik Kumara, N. K. Lokanath, Ray J. Butcher, Vijay Kumbar, Kishore Bhat
Summary: A novel 1,8-naphthalimide-containing ligand and its three mononuclear complexes were synthesized and characterized. The ligand exhibited a non-planar structure with π···π stacking interaction, while the complexes showed a seesaw geometry. The complexes exhibited DNA binding in an intercalative mode and demonstrated improved cytotoxicity towards cancer cells.
NEW JOURNAL OF CHEMISTRY
(2022)
