Article
Biochemistry & Molecular Biology
Qianhong Li, Chandrashekhar Dasari, Ding Li, Asma Arshia, Ahmed Muaaz Umer, Mohamed Riad Abdelgawad Abouzid, Yiru Guo, Roberto Bolli
Summary: The study found that HO-1 significantly influences the proliferation and DNA synthesis of CPCs, with Nrf2 promoting nuclear translocation possibly being the key regulator of antioxidant genes by HO-1. Upregulation of Ec-SOD by HO-1 in CPCs benefits the survival and reparative ability of CPCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Jin-Ock Kim, Kwang-Hyeok Kim, Eun Ji Baek, Bomi Park, Min Kyung So, Byoung Joon Ko, Han-Jik Ko, Sang Gyu Park
Summary: The study developed an anti-c-Kit antibody-drug conjugate, NN2101-DM1, which showed potent growth-inhibitory activities against c-Kit-positive cancer cell lines, especially in cases of TKI resistance. In mouse xenograft models, NN2101-DM1 exhibited strong growth-inhibitory activities against imatinib-resistant GIST and SM cells, and a significantly higher anti-cancer effect than carboplatin/etoposide against SCLC cells. Combination of NN2101-DM1 with imatinib in imatinib-sensitive GIST cells induced complete remission in mouse xenograft models, suggesting its potential as a therapeutic agent for wild-type and mutant c-Kit-positive cancers.
MOLECULAR ONCOLOGY
(2022)
Article
Cell Biology
Suneel Rallapalli, Soma Guhathakurta, Purna S. Korrapati
Summary: The discovery of CPCs has raised hopes for heart cell therapy, but there are still issues with defining CPC types and validating methods for generating high-quality CPC populations. Long-term culture may alter CPC immunophenotypes, highlighting the need for validated molecular signatures to assess the impact of ex vivo expansion on cell therapy products.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Dong Mei Xie, Yang Chen, Yan Liao, Wanwen Lin, Gang Dai, Di Han Lu, Shuanghua Zhu, Ke Yang, Bingyuan Wu, Zhihong Chen, Chaoquan Peng, Mei Hua Jiang
Summary: The study found that CD51 can serve as a marker for resident cardiac MSCs, and CD51(+)cMSC therapy enhances cardiac repair through promoting angiogenesis and a mechanism mediated by SCF.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Hideki Kobayashi, Shugo Tohyama, Hideaki Kanazawa, Hajime Ichimura, Shuji Chino, Yuki Tanaka, Yota Suzuki, Jian Zhao, Naoko Shiba, Shin Kadota, Kazumasa Narita, Takafumi Naito, Tatsuichiro Seto, Koichiro Kuwahara, Yuji Shiba, Keiichi Fukuda
Summary: Advancements in stem cell biology have allowed for cardiac regeneration through the use of human pluripotent stem cell-derived cardiomyocytes (PSC-CMs). However, current transplantation methods involving PSC-CMs have limitations such as poor engraftment and the need for invasive procedures. This study investigated the feasibility of intracoronary transplantation of PSC-CMs in cynomolgus monkeys. The results showed low efficacy of this approach, with no coronary embolism or graft survival, and scar formation due to cardiac ischemic injury.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2023)
Review
Engineering, Biomedical
Brian Kato, Gary Wisser, Devendra K. Agrawal, Tim Wood, Finosh G. Thankam
Summary: Advanced three-dimensional bioprinting technology shows promise in aiding cardiac tissue repair and potentially offering an alternative to organ transplantation. Despite facing challenges, there is still hope for the future.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2021)
Review
Cell & Tissue Engineering
Teng Li, Chengxin Luo, Jiasi Zhang, Ling Wei, Wei Sun, Qin Xie, Yan Liu, Yongli Zhao, Shuangnian Xu, Lihua Wang
Summary: The co-infusion of mesenchymal stem cells (MSCs) in allogeneic hematopoietic stem cell transplantation (allo-HSCT) has shown improvement in engraftment and reduced risk of chronic graft-versus-host disease (cGVHD). However, the effects varied among different age and disease groups, suggesting potential benefits for certain populations.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Medicine, General & Internal
Louyi Shen, Guan Fan, Guoliang Yang, Zhijie Yang, Chun Gui
Summary: This study aimed to investigate the effects of differentially expressed miRNAs on the function of CPCs under hypoxia. It was found that miR-210-3p was significantly increased in hypoxia-treated CPCs and it enhanced the angiogenic capability of CPCs by regulating the secretion of angiogenic factors. This suggests that miR-210-3p may play a crucial role in promoting angiogenesis in ischemic heart disease.
ANNALS OF MEDICINE
(2023)
Review
Zoology
Jiao Ma
Summary: Myocardial cell loss is hypothesized to be irreversible, but recent research suggests that human cardiac stem cells (hCSCs) may have therapeutic potential for cardiac repair. The properties and regulatory mechanisms of hCSC development have yet to be fully explored. Different types of cardiac stem cells with distinct molecular phenotypes and differentiation potentials have been discovered and studied. Factors such as paracrine and autocrine factors, extracellular matrixes, and genetic factors can regulate the proliferation and differentiation of cardiac stem cells, which play a significant role in repairing heart injuries. Cytokines and growth factors have been found to enhance endogenous cardiac stem cell proliferation and migration capacity. Combining these factors with cardiac stem cells has shown promise in improving the efficiency of heart injury repair.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Wannhsin Chen, Chun-Hsiang Hou, Yi-Ling Chen, Hsin-Hsin Shen, Chen-Hsuan Lin, Cheng-Yi Wu, Meng-Hsueh Lin, Chih-Ching Liao, Jun-Jae Huang, Chi-Yu Yang, Yi-Chen Li, Hon-Kan Yip
Summary: This study aimed to test the safety and effectiveness of early intracoronary injection of human bone marrow-derived mesenchymal stem cells (OmniMSCs) in pigs with acute ST-segment elevation myocardial infarction. The results showed that OmniMSCs treatment effectively reduced the infarct size and preserved left ventricular function.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Review
Physiology
Maria Cimini, Raj Kishore
Summary: New insights into the cellular and extra-cellular composition of scar tissue after myocardial infarction have identified a heterogeneous podoplanin-expressing cell population associated with fibrogenic and inflammatory responses during scar formation. Podoplanin, mainly expressed by cardiac lymphatic endothelial cells in adult mouse hearts, is unexpectedly acquired by different types of cells after MI, suggesting an activation of progenitor cells during post-ischemic myocardial wound repair. Inhibition of the interaction between podoplanin-expressing cells and podoplanin-binding cells using podoplanin-neutralizing antibodies showed reduced inflammation post-MI and improved heart function and scar composition.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Kylie Crompton, Iona Novak, Michael Fahey, Nadia Badawi, Katherine J. Lee, Francoise Mechinaud-Heloury, Priya Edwards, Paul Colditz, Trisha Soosay Raj, Janet Hough, Xiaofang Wang, Simon Paget, Kuang-Chih Hsiao, Peter Anderson, Dinah Reddihough
Summary: This study investigated the safety and preliminary efficacy of sibling cord blood cell infusion in children with cerebral palsy. The findings suggest that sibling cord blood cell infusion is relatively safe for children with cerebral palsy when conducted in appropriate facilities, but the improvement in motor function and upper extremity skills is limited.
Article
Cell & Tissue Engineering
David Brett Erasmus, Nisha Durand, Francisco A. Alvarez, Tathagat Narula, David O. Hodge, Abba C. Zubair
Summary: This study demonstrates the therapeutic effects of intravenous infusion of low-dose bone marrow-derived MSCs in lung transplant patients. The results show that this treatment can slow the progression of chronic lung allograft syndrome in some patients and stabilize lung function decline.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Ayesha M. Yusuf, Rizwan Qaisar, Abaher O. Al-Tamimi, Manju Nidagodu Jayakumar, James R. Woodgett, Walter J. Koch, Firdos Ahmad
Summary: The loss of cardiomyocyte-GSK-3 beta promotes cardiomyocyte and resident CPC proliferation post-myocardial infarction, potentially regulated by dysregulated growth factors post-MI. Inhibiting cardiomyocyte-GSK-3 beta could be a helpful strategy for in situ cardiac regeneration post-ischemic injury.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Siyuan Ma, Junyu Yan, Lu Chen, Yingqi Zhu, Kaitong Chen, Cankun Zheng, Mengjia Shen, Yulin Liao
Summary: Recent research suggests that cardiac regeneration has the potential to treat heart failure. The field is rapidly developing, with key topics including cardiomyocyte proliferation, stem cells, exosomes, cardiac reprogramming, and macrophages. The United States and Harvard University are leading in research output in this area.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Editorial Material
Cardiac & Cardiovascular Systems
Kendra J. Grubb, Gaetano Paone
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Jonathan R. Zurcher, Kendra J. Grubb
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2023)
Article
Biochemistry & Molecular Biology
Mark A. Doll, Andrew R. Ray, Raul A. Salazar-Gonzalez, Parag P. Shah, Alexis A. Vega, Sophia M. Sears, Austin M. Krueger, Kyung U. Hong, Levi J. Beverly, David W. Hein
Summary: Arylamine N-acetyltransferase 1 (NAT1) is frequently upregulated in breast cancer. Inhibition or reduction of NAT1 in breast cancer cells does not consistently decrease cell growth, migration, or invasion in vitro. However, in an in vivo xenograft model, NAT1 knockout (KO) breast cancer cells produce smaller primary tumors due to increased apoptosis, but do not show alterations in lung metastasis frequency.
MOLECULAR CARCINOGENESIS
(2022)
Article
Cardiac & Cardiovascular Systems
Keti Vitanova, Syed Zaid, Gilbert H. L. Tang, Tsuyoshi Kaneko, Vinayak N. Bapat, Thomas Modine, EXPLANT TAVR Investigators
Summary: In this study, the outcomes of AVR and root replacement after TAVR explantation were compared. The results showed no differences in short-term mortality and morbidities between the two groups, although they had different clinical characteristics.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2023)
Article
Oncology
Kyung U. Hong, Afi H. Tagnedji, Mark A. Doll, Kennedy M. Walls, David W. Hein
Summary: This study observed the upregulation of cytidine deaminase (CDA) in multiple breast cancer cell lines due to NAT1 deletion. It was found that NAT1 KO cells showed increased sensitivity to 5fdC, which depended on their increased CDA activity. These findings provide a new therapeutic strategy for treating breast cancer with elevated NAT1 expression.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Toxicology
Kyung U. Hong, Raul A. Salazar-Gonzalez, Kennedy M. Walls, David W. Hein
Summary: This study demonstrates, for the first time, that human NAT2 is transcriptionally regulated by glucose and insulin in liver cancer cell lines and that the gene expression pattern of NAT2 is similar to that of genes involved in lipid metabolism and transport.
TOXICOLOGICAL SCIENCES
(2022)
Article
Peripheral Vascular Disease
Alok R. Amraotkar, Ugochukwu S. Owolabi, Marina Malovichko, Sana Majid, Robert M. Weisbrod, Emelia J. Benjamin, Jessica L. Fetterman, Glenn A. Hirsch, Sanjay Srivastava, Ram Poudel, Rose Marie Robertson, Aruni Bhatnagar, Naomi M. Hamburg, Rachel J. Keith
Summary: This study aimed to assess the association between e-cigarette use and vascular health. The results showed that dual use of e-cigarettes and combustible cigarettes was associated with higher levels of endothelial origin circulating angiogenic cells (CACs), indicating vascular injury. Sole use of e-cigarettes was associated with higher levels of endothelial and inflammatory CACs, suggesting ongoing systemic injury. Therefore, CACs may serve as informative biomarkers for evaluating the effects of tobacco product use on vascular health.
Article
Cardiac & Cardiovascular Systems
Daniel O'Hair, Steven J. Yakubov, Kendra J. Grubb, Jae K. Oh, Saki Ito, G. Michael Deeb, Nicolas M. Van Mieghem, David H. Adams, Tanvir Bajwa, Neal S. Kleiman, Stanley Chetcuti, Lars Sondergaard, Hemal Gada, Mubashir Mumtaz, John Heiser, William M. Merhi, George Petrossian, Newell Robinson, Gilbert H. L. Tang, Joshua D. Rovin, Stephen H. Little, Renuka Jain, Sarah Verdoliva, Tim Hanson, Shuzhen Li, Jeffrey J. Popma, Michael J. Reardon
Summary: This study aimed to evaluate the 5-year incidence and clinical outcomes of hemodynamic structural valve deterioration (SVD) in patients undergoing self-expanding transcatheter aortic valve implantation (TAVI) or surgery. The results showed a lower rate of SVD in the TAVI group and its association with worse clinical outcomes.
Article
Multidisciplinary Sciences
Kyung U. Hong, Jonathan Q. Gardner, Mark A. Doll, Marcus W. Stepp, Daniel W. Wilkey, Frederick W. Benz, Jian Cai, Michael L. Merchant, David W. Hein
Summary: Arylamine N-acetyltransferase 1 (NAT1) is frequently upregulated in breast cancer. In this study, proteomic analysis of NAT1 knockout cells compared to parental breast cancer cells revealed proteins associated with MHC major histocompatibility complex I-mediated antigen presentation as significantly upregulated, while multiple processes involved in mitochondrial functions were collectively downregulated. This dataset provides valuable information for further exploring the role of NAT1 in breast cancer.
Editorial Material
Medicine, General & Internal
Alok R. Amraotkar, Rachel J. Keith, Kenneth E. Palmer, Aruni Bhatnagar
AMERICAN JOURNAL OF MEDICINE
(2023)
Article
Toxicology
Kennedy M. Walls, Kyung U. Hong, David W. Hein
Summary: Heterocyclic amines (HCAs) are mutagenic substances that can be consumed through cooked meat, and recent studies have found a significant association between dietary HCA exposure and insulin resistance and type II diabetes. This study investigated the effects of three HCAs commonly found in cooked meat on insulin signaling and glucose production in human hepatocytes, and found that HCA exposure decreases insulin signaling and promotes hepatic glucose production.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kyung U. Hong, David W. Hein
Summary: A novel NAT2 haplotype composed of seven non-coding variants has been linked to dyslipidemia and bladder cancer. The risk alleles of this haplotype are associated with different levels of NAT2 gene expression, suggesting a role in modifying the risk of these diseases.
PHARMACOGENETICS AND GENOMICS
(2023)
Review
Pharmacology & Pharmacy
Kyung U. Hong, Kennedy M. Walls, David W. Hein
Summary: Arylamine N-acetyltransferase 2 (NAT2) is an important metabolic enzyme involved in the metabolism of aromatic amines and hydrazines. Genetic variants in the coding region of NAT2 can affect enzyme activity and protein stability, leading to different acetylator phenotypes. Recent genome wide association studies have discovered non-coding or intergenic variants of NAT2 that are associated with elevated plasma lipid and cholesterol levels, suggesting a new role of NAT2 in lipid and cholesterol homeostasis.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Toxicology
Kennedy M. Walls, Kyung U. Hong, David W. Hein
Summary: Heterocyclic amines (HCAs) found in cooked meat have been associated with insulin resistance and type II diabetes. HCAs induce insulin resistance and glucose production in human hepatocytes, and this effect may be more pronounced in individuals with the rapid NAT2 acetylator phenotype.
TOXICOLOGY LETTERS
(2023)
Review
Biochemistry & Molecular Biology
Noel Verjan Garcia, Kyung U. Hong, Nobuyuki Matoba
Summary: The endoplasmic reticulum (ER) plays a vital role in cell homeostasis and disruptions to its functions can lead to diseases. Dysregulated ER stress and UPR are linked to various human diseases, including inflammatory bowel disease (IBD). A recent study has shown that EPICERTIN can induce a protective UPR in colon epithelial cells, promoting mucosal healing in IBD models.