Review
Biotechnology & Applied Microbiology
Yun-Feng Zhou, Di Zhang, Wan-Ting Yan, Kai Lian, Zheng-Zheng Zhang
Summary: This article reviews the therapeutic potential of multipotent stromal cells (MSCs) in repairing meniscal injuries and discusses the avenues for utilizing these cells. The current progress on biomaterial implants is also reviewed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
S. Marsh, T. Constantin-Teodosiu, V. Chapman, V. Sottile
Summary: The increasing prevalence of joint disease, especially osteoarthritis, requires new treatment strategies. Mesenchymal stem cells (MSCs) show promise for tissue repair, but their functional integration into host tissue may be affected by the inflammatory environment. This study evaluates the effects of inflammatory mediators on MSCs derived from bone marrow and provides insight into the impact of factors driving osteoarthritis pathology on the therapeutic potential of MSCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell & Tissue Engineering
Hesham Soliman, Marine Theret, Wilder Scott, Lesley Hill, Tully Michael Underhill, Boris Hinz, Fabio M. Rossi
Summary: MSCs are essential for the development, maintenance, function, and regeneration of most tissues. They play a crucial role in responding to injury, promoting regeneration through secretion of trophic factors and ECM molecules, and contributing to fibrotic reparative processes. Through fate mapping and single cell omics, tissue-specific MSC identities and functions are being elucidated, providing unprecedented insights into the secret lives of tissue-resident MSCs.
Review
Cell Biology
Vadim I. Chechekhin, Konstantin Yu. Kulebyakin, Romesh I. Kokaev, Pyotr A. Tyurin-Kuzmin
Summary: Adipose tissue relies on the regulation of multipotent mesenchymal stromal cells (MSCs), which serve as both precursors for new adipocytes and key paracrine regulators of adipose tissue homeostasis. The activity of MSCs is tightly controlled, regulating aspects such as proliferation, differentiation, and signal molecule production through modulation of hormonal sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Makram Merimi, Karolien Buyl, Dhouha Daassi, Robim M. Rodrigues, Rahma Melki, Philippe Lewalle, Tamara Vanhaecke, Hassan Fahmi, Vera Rogiers, Laurence Lagneaux, Joery De Kock, Mehdi Najar
Summary: This study investigates the response of AT-MSCs to inflammation and cell-passaging, revealing that inflammation leads to upregulation of specific cytokines and TLR, while cell-passaging increases the expression of other genes. These findings provide insights into the characteristics of AT-MSCs and potential therapeutic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Sofia Avnet, Silvia Lemma, Margherita Cortini, Gemma Di Pompo, Francesca Perut, Maria Veronica Lipreri, Laura Roncuzzi, Marta Columbaro, Costantino Errani, Alessandra Longhi, Nicoletta Zini, Dominique Heymann, Massimo Dominici, Giulia Grisendi, Giulia Golinelli, Lorena Consolino, Dario Livio Longo, Cristina Nanni, Alberto Righi, Nicola Baldini
Summary: The study validated the correlation between tumor glycolysis/acidosis and metastasis, and investigated the role of acidity-induced inflammation in the development of metastasis in osteosarcoma.
Article
Cell Biology
Janina Burk, Michaela Melzer, Alina Hagen, Katrin Susanne Lips, Katja Trinkaus, Ariane Nimptsch, Jenny Leopold
Summary: This study investigated the applicability of lipid phenotyping for MSC characterization and discrimination, finding differences in phospholipid species diversity between MSC from different species, and identified potentially suitable lipid markers across culture conditions. Phospholipid profiling could be a promising approach for MSC characterization, allowing for some heterogeneity within MSC while still achieving clear discrimination from fibroblasts.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
A. Hagen, H. Lehmann, S. Aurich, N. Bauer, M. Melzer, J. Moellerberndt, V. Patane, C. L. Schnabel, J. Burk
Summary: This study aimed to establish a scalable protocol for equine platelet lysate (ePL) production and to test its effectiveness in equine MSC culture. Platelet concentrates prepared using the buffy coat method showed increased platelet and reduced white blood cell content, and were used successfully as a supplement in MSC culture. Further research into the functionality of equine MSC with ePL as a culture medium should be conducted.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Polymer Science
Irena Vackova, Eliska Vavrinova, Jana Musilkova, Vojtech Havlas, Yuriy Petrenko
Summary: Clinical application of tissue-engineered grafts requires compliance with regulatory requirements in cell processing, biomaterial scaffold selection, and quality control analysis. This study investigates the effects of low-temperature storage on the functional properties of cells in 3D constructs using Wharton's jelly multipotent mesenchymal stromal cells (WJ-MSCs) and the clinically-acceptable HyaloFast scaffold.
Article
Chemistry, Multidisciplinary
Jonathan O. Martinez, Michael Evangelopoulos, Ava A. Brozovich, Guillermo Bauza, Roberto Molinaro, Claudia Corbo, Xuewu Liu, Francesca Taraballi, Ennio Tasciotti
Summary: The study introduces a versatile multicomponent delivery platform that combines MSC tropism with MSV-mediated payload delivery, demonstrating increased drug release and reduced inflammation, resulting in improved outcomes in experimental models.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Hematology
Elien Sergeant, Malicorne Buysse, Timothy Devos, Ben Sprangers
Summary: Bone marrow-derived multipotent mesenchymal stromal cells have the potential to modulate immune responses and promote immune tolerance in kidney transplant recipients. However, caution is needed regarding their clinical use, as more detailed analysis of the mechanisms for transplant tolerance induction and factors affecting their functionality is required.
Review
Biochemistry & Molecular Biology
Gyuzyal Tabeeva, Denis Silachev, Polina Vishnyakova, Alexandra Asaturova, Timur Fatkhudinov, Antonina Smetnik, Madina Dumanovskaya
Summary: Disruption of endometrial regeneration, fibrosis formation, and intrauterine adhesions are common causes of thin endometrium and/or Asherman's syndrome, leading to infertility and adverse obstetric outcomes. Current methods do not restore the regenerative properties of the endometrium. Cell therapy using MMSCs has shown promising results in tissue damage repair, particularly through paracrine effects of EVs secreted by MMSCs, which stimulate progenitor cells and exert cytoprotective, antiapoptotic, and angiogenic effects. This review provides information on the regulatory mechanisms of endometrial regeneration, associated pathological conditions, and the role of MMSCs and EVs in endometrial repair and reproductive processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Shiqi Xiang, Zixuan Lin, Meagan J. J. Makarcyzk, Kanyakorn Riewruja, Yiqian Zhang, Xiurui Zhang, Zhong Li, Karen L. Clark, Eileen Li, Silvia Liu, Tingjun Hao, Madalyn R. R. Fritch, Peter G. G. Alexander, Hang Lin
Summary: This study elucidates the difference between hiMPCs and hMSCs in response to TGF beta and presents a robust method for obtaining low hypertrophy hiMPCs-derived cartilage. The results also indicate the importance of activating both Smad2/3 and Smad1/5 in the initiation of chondrogenesis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Sadovskaya, Nataliya Petinati, Nina Drize, Igor Smirnov, Olga Pobeguts, Georgiy Arapidi, Maria Lagarkova, Alexander Belyavsky, Anastasia Vasilieva, Olga Aleshina, Elena Parovichnikova
Summary: In patients with AML, the properties of MSCs are modified, reducing their ability to maintain normal hematopoiesis. This study analyzed the secretomes of MSCs from AML patients and healthy donors to understand the role of MSCs in supporting leukemia cells and restoring normal hematopoiesis. The analysis showed that there were significant differences in the secretomes of AML patient MSCs compared to healthy donors, with changes in proteins related to ossification, transport, and immune response. Despite remission, the impaired functions of MSCs remained, indicating irreversible changes caused by AML.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Yuko Nitahara-Kasahara, Mutsuki Kuraoka, Yuki Oda, Hiromi Hayashita-Kinoh, Shin'ichi Takeda, Takashi Okada
Summary: The study focused on the anti-inflammatory properties of IL-10 and investigated the potential benefits of IL-10-expressing MSCs. Results showed that local injection of IL-10-MSCs maintained long-term engraftment and facilitated tissue repair, while repeated systemic administration protected muscles from damage and improved muscle dysfunction in DMD. The paracrine effects of IL-10, especially involving SDF-1, offer potential benefits for DMD therapy.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Orthopedics
Helga Joos, Frank Leucht, Jana Riegger, Cathrin Hogrefe, Joerg Fiedler, Lutz Duerselen, Heiko Reichel, Anita Ignatius, Rolf E. Brenner
AMERICAN JOURNAL OF SPORTS MEDICINE
(2015)
Article
Cell & Tissue Engineering
Elisa Maria Amann, Markus Thomas Rojewski, Sinja Rodi, Daniel Fuerst, Joerg Fiedler, Annette Palmer, Sonja Braumueller, Markus Huber-Lang, Hubert Schrezenmeier, Rolf Erwin Brenner
Article
Rheumatology
E. M. Schneider, W. Du, J. Fiedler, J. Hoegel, K. P. Guenther, H. Brenner, R. E. Brenner
ANNALS OF THE RHEUMATIC DISEASES
(2011)
Article
Engineering, Biomedical
Joerg Fiedler, Burcin Oezdemir, Jochen Bartholomae, Alfred Plettl, Rolf E. Brenner, Paul Ziemann
Article
Engineering, Biomedical
Joerg Fiedler, Andreas Kolitsch, Bernhard Kleffner, Dietmar Henke, Steffen Stenger, Rolf E. Brenner
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
(2011)
Article
Medicine, Research & Experimental
Joerg Fiedler, Juergen Groll, Erika Engelhardt, Peter Gasteier, Claudia Dahmen, Horst Kessler, Martin Moeller, Rolf E. Brenner
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2011)
Article
Engineering, Biomedical
Ludwika Kreja, Astrid Liedert, Heiter Schlenker, Rolf E. Brenner, Joerg Fiedler, Benedikt Friemert, Lutz Duerselen, Anita Ignatius
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2012)
Article
Engineering, Biomedical
Carl Neuerburg, Stefan Recknagel, Joerg Fiedler, Juergen Groll, Martin Moeller, Kristina Bruellhoff, Heiko Reichel, Anita Ignatius, Rolf E. Brenner
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2013)
Article
Biochemistry & Molecular Biology
Nina-Emily Hengartner, Joerg Fiedler, Anita Ignatius, Rolf E. Brenner
MOLECULAR MEDICINE
(2013)
Article
Multidisciplinary Sciences
Julia V. Woelfle, Joerg Fiedler, Lutz Duerselen, Judith Reichert, Dieter Scharnweber, Anne Foerster, Bernd Schwenzer, Heiko Reichel, Anita Ignatius, Rolf E. Brenner
Article
Multidisciplinary Sciences
Annika Kasten, Tamara Naser, Kristina Bruellhoff, Joerg Fiedler, Petra Mueller, Martin Moeller, Joachim Rychly, Juergen Groll, Rolf E. Brenner
Article
Chemistry, Physical
J. Woelfle-Roos, Katmer B. Amet, J. Fiedler, H. Michels, G. Kappelt, A. Ignatius, L. Duerselen, H. Reichel, R. E. Brenner
Article
Materials Science, Multidisciplinary
Fiedler Joerg, Katmer Amet Betuel, Michels Heiner, Kappelt Gerhard, Brenner Rolf Erwin
Article
Cell Biology
Hanna Tritschler, Konrad Fischer, Jochen Seissler, Joerg Fiedler, Rebecca Halbgebauer, Markus Huber-Lang, Angelika Schnieke, Rolf E. Brenner
Summary: Transplantation of xenogenic porcine chondrocytes for the treatment of human articular cartilage defects face challenges due to humoral and cellular rejection triggered by xenogenic epitopes. Genetic modifications in pigs, including knockout of genes responsible for xenogenic epitope biosynthesis and transgenic expression of human complement inhibitors, have shown promise in preventing hyperacute xenograft rejection and improving chondrocyte viability.
