Article
Medicine, Research & Experimental
Xiuhui Chen, Wen Zhong, Yue Chang, Tiefang Song, Botong Liu, Xianchao Kong, Qingfei Kong
Summary: This study investigated the potential effects of mitochondria derived from menstrual stem cells (MenSCs) on ovarian cancer. Proteomic analysis revealed that 592 proteins were increased in MenSC-derived mitochondria, mainly enriched in metabolism-associated oxidative phosphorylation pathway. Further analysis showed similar increasing trends for certain proteins in the oxidative phosphorylation pathway. These findings suggest that mitochondria from MenSCs may enhance ovarian cancer progression through the enrichment of oxidative phosphorylation-associated pathways.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jingshu Liu, Jiangfeng Qiu, Zhiqi Zhang, Lei Zhou, Yunzhe Li, Dongyan Ding, Yang Zhang, Dongling Zou, Dong Wang, Qi Zhou, Tingyuan Lang
Summary: Overexpression of SOX4 promotes stemness of colorectal cancer cells by upregulating CSC markers and inducing proteome changes, including activation of HDAC1 as a key mechanism. The SOX4-HDAC1 axis is conserved in multiple types of cancer, suggesting HDAC1 is a potential drug target for eliminating SOX4-driven human CSCs.
CELL AND BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Anastasia Kotova, Arseniy A. Lobov, Julia A. Dombrovskaya, Valentina Y. Sannikova, Nadezhda A. Ryumina, Polina Klausen, Alexey L. Shavarda, Anna B. Malashicheva, Natella Enukashvily
Summary: Dental stem cells, derived from neural crest stem cells, exhibit differences in functional and biological properties, including neural markers, cell morphology, proliferation rate, and osteogenic differentiation capabilities. Dental pulp stem cells and periodontal ligament stem cells show distinctions in neural markers, nuclear protein expression, proliferation rate, etc.
Review
Oncology
Ying Yang, Wen-Jian Meng, Zi-Qiang Wang
Summary: Gastric cancer is a common malignancy with poor prognosis. Gastric cancer stem cells (GCSCs) are closely associated with drug resistance, recurrence, and metastasis, indicating their potential as therapeutic targets for gastric cancer. However, the relationship and mechanism between GCSCs and gastric cancer still require further exploration.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Masayuki Komatsu, Hitoshi Ichikawa, Fumiko Chiwaki, Hiromi Sakamoto, Rie Komatsuzaki, Makoto Asaumi, Kazuhisa Tsunoyama, Takeo Fukagawa, Hiromichi Matsushita, Narikazu Boku, Keisuke Matsusaki, Fumitaka Takeshita, Teruhiko Yoshida, Hiroki Sasaki
Summary: Frequent gene fusions of ARHGAP6/ARHGAP26 are found in peritoneally-metastasized gastric and pancreatic cancer, leading to downregulation of RhoA-ROCK-MLC2 signaling and cell death. The findings suggest the tumor-suppressive nature of ARHGAP-RhoA signaling and may provide a new avenue for drug discovery against this refractory cancer.
Article
Medicine, General & Internal
Hugo Osorio, Catia Silva, Marta Ferreira, Irene Gullo, Valdemar Maximo, Rita Barros, Fernando Mendonca, Carla Oliveira, Fatima Carneiro
Summary: This study characterized the proteomic profile of gastric cancer in patients with type 2 diabetes mellitus, revealing differentially expressed proteins associated with various biological processes and diseases. The results supported the association between diabetes, metabolic disorders, and related diseases, providing valuable insights into potential protein targets for further investigation.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Maddalen Otaegi-Ugartemendia, Ander Matheu, Estefania Carrasco-Garcia
Summary: Therapy resistance is a major obstacle in cancer cure, and gastric cancer stem cells (gCSCs) play a crucial role in this resistance. They develop protective mechanisms such as quiescence, enhanced detoxification and drug efflux activity, which make them resistant to current treatments. Although several regulators have been identified, there is currently no effective therapeutic strategy targeting gCSCs.
Article
Pharmacology & Pharmacy
Peiheng Cai, Gaofan Sheng, Shiqin Jiang, Daifei Wang, Zhongxiang Zhao, Min Huang, Jing Jin
Summary: This study revealed the molecular mechanism of how Cryptotanshinone (CTS) enhances the sensitivity of lung cancer H1975 cells to gefitinib using a proteomic approach. The results demonstrated that CTS mainly enhances the efficacy of gefitinib by impacting the redox process and fatty acid metabolism in H1975 cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Xionghui Rao, Chaojun Zhang, Huixing Luo, Jianbao Zhang, Zhehong Zhuang, Zhihao Liang, Xiaobin Wu
Summary: Gastric cancer is the fourth deadliest cancer globally, mainly due to metastasis, recurrence, and chemotherapy resistance. Cancer stem cells play a key role in these processes, and targeting gastric cancer stem cells is considered an effective treatment method. Genes and noncoding RNAs are important regulatory factors for GCSCs, and regulating these factors can target the stemness of gastric cancer, providing new directions for clinical treatment.
Review
Oncology
Ying Yang, Wen-Jian Meng, Zi-Qiang Wang
Summary: This review examines the effects of the tumor microenvironment and cancer stem cells on the progression and therapy resistance of gastric cancer, highlighting the potential for developing successful anti-tumor therapy by targeting the tumor microenvironment and cancer stem cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemical Research Methods
Benjamin C. Orsburn
Summary: Mutations in the KRAS gene are common drivers of cancer in humans. This study discovered a promising compound, MRTX1133, that inhibits the activity of KRAS(G12D) mutant proteins in pancreatic cancer cells. Through proteomics and metabolomics analysis, it was found that after 48 hours of treatment, the compound showed different levels of effectiveness in reducing the abundance of KRAS protein in different cell populations.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Cell Biology
Ruihong Zhang, Luguang Liu, Fengqin Wang, Weizhu Zhao, Kai Liu, Hang Yu, Siwei Zhao, Botao Xu, Xiaoli Zhang, Jie Chai, Jing Hao
Summary: AKAP-8L is an upregulated gene in chemoresistant gastric cancer (GC) tissues. Higher AKAP-8L expression is associated with lower survival rate in GC patients. Overexpression of AKAP-8L enhances the stemness and chemoresistance of GC cells. SCD1 plays a critical role in mediating AKAP-8L's function in GC cell stemness and chemoresistance.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Zeyang Wang, Zhi Lv, Qian Xu, Liping Sun, Yuan Yuan
Summary: This study identified 137 differentially expressed proteins in EBVaGC tissue using EBER ISH and DIA mass spectrometry, constructed a protein-protein interaction network, and identified potential hub genes. Gene enrichment analysis indicated involvement in energy and biochemical metabolism pathways. The highly associated protein GBP5 with EBVaGC was validated and further analysis suggested its association with clinicopathological parameters and EBV infection in GC.
CANCER CELL INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Giovanni Chiappetta, Tania Gamberi, Fiorella Faienza, Xhesika Limaj, Salvatore Rizza, Luigi Messori, Giuseppe Filomeni, Alessandra Modesti, Joelle Vinh
Summary: This study investigated the effects of Auranofin (AF) on protein expression and protein oxidation in A2780 cancer cells using a strategy based on simultaneous expression proteomics and redox proteomics determinations. The results demonstrated that AF affected the cellular redox state, mitochondrial functions, and induced ER stress, which were extensively validated. The study suggests that AF exerts its effects through a multitarget mechanism, mainly affecting redox metabolism and mitochondrial functions.
Review
Oncology
Martina Addeo, Giuseppina Di Paola, Henu Kumar Verma, Simona Laurino, Sabino Russi, Pietro Zoppoli, Geppino Falco, Pellegrino Mazzone
Summary: Gastric cancer stem cells play a crucial role in the initiation and progression of gastric cancer, with features like self-renewal capability and resistance to current anticancer therapies. Targeting metabolic pathways of cancer stem cells could be a promising strategy in cancer therapy.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Anna Kakehashi, Arpamas Chariyakornkul, Shugo Suzuki, Napaporn Khuanphram, Kumiko Tatsumi, Shotaro Yamano, Masaki Fujioka, Min Gi, Rawiwan Wongpoomchai, Hideki Wanibuchi
Summary: In this study, novel early molecular biomarkers of liver neoplasms in non-alcoholic steatohepatitis (NASH) were discovered using the STAM mouse model. The protein CACHD1 was found to be significantly elevated in hepatocellular carcinomas, suggesting its potential as a biomarker and molecular target in NASH-associated hepatocarcinogenesis involving cell proliferation, autophagy, and apoptosis. The research highlights CACHD1 as an early biomarker of liver preneoplastic and neoplastic lesions in the context of NASH, with implications for potential therapeutic targets in diabetes mellitus/NASH-associated hepatocarcinogenesis.
Article
Ophthalmology
Mizuki Tagami, Anna Kakehashi, Atsushi Sakai, Norihiko Misawa, Atsuko Katsuyama-Yoshikawa, Hideki Wanibuchi, Atsushi Azumi, Shigeru Honda
Summary: This study investigated thrombospondin-1 expression in conjunctival squamous cell carcinoma and found it to be associated with tumor progression and patient prognosis. Thrombospondin-1 may serve as a potential molecular target and prognostic factor in conjunctival SCC.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Article
Oncology
Anna Kakehashi, Shugo Suzuki, Masayuki Shiota, Nina Raymo, Min Gi, Taro Tachibana, Vasily Stefanov, Hideki Wanibuchi
Summary: Protein canopy homolog 2 (CNPY2) plays a crucial role in controlling neurite outgrowth and is significantly associated with cell cycle progression, proliferation, survival, and invasion in liver cancer cells. Its elevation in HCV-associated HCC patients is positively correlated with survival, suggesting it as a potential prognostic marker for liver cancer.
Article
Multidisciplinary Sciences
Mizuki Tagami, Anna Kakehashi, Atsuko Katsuyama-Yoshikawa, Norihiko Misawa, Atsushi Sakai, Hideki Wanibuchi, Atsushi Azumi, Shigeru Honda
Summary: This study examined the role of regulatory T cell activity in tumor immunity in conjunctival squamous cell carcinoma (SCC) and found that FOXP3 and the CXCR4/FOXP3 axis are important pathological and prognostic factors. The findings suggest that the tumor microenvironment should be considered in future treatment options for conjunctival SCC.
Article
Oncology
Taisuke Matsue, Min Gi, Masayuki Shiota, Hirokazu Tachibana, Shugo Suzuki, Masaki Fujioka, Anna Kakehashi, Tomoki Yamamoto, Minoru Kato, Junji Uchida, Hideki Wanibuchi
Summary: In this study, the functional association of carbonic anhydrases (CA) with the development and invasion of urothelial carcinoma (UC) was investigated. It was found that overexpression of CA2 is associated with invasion and progression of UC. Treatment with the CA inhibitor acetazolamide (Ace) significantly decreased the incidence of UC induced by N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) and inhibited muscle invasion. The inhibition of CA2 by Ace also suppressed migration and invasion capabilities of UC cells. These findings confirm CA2 as a potential therapeutic target for bladder cancer.
Article
Oncology
Tomoki Yamamoto, Min Gi, Satoshi Yamashita, Shugo Suzuki, Masaki Fujioka, Arpamas Vachiraarunwong, Runjie Guo, Guiyu Qiu, Anna Kakehashi, Minoru Kato, Junji Uchida, Hideki Wanibuchi
Summary: In this study, aberrant DNA methylation and gene expression changes were identified in DMAV-induced rat bladder urothelial carcinoma. The findings suggest that these alterations occurred in the early stages of arsenic-induced bladder carcinogenesis, highlighting the importance of aberrant DNA methylation in this process. Further investigation into the functions of these genes will enhance our understanding of the role of DNA methylation in arsenic-related bladder cancers and aid in the identification of potential therapeutic targets.
Review
Oncology
Anna Kakehashi, Shugo Suzuki, Hideki Wanibuchi
Summary: This review focuses on recent research in the field of non-alcoholic fatty liver disease (NAFLD) or metabolic dysfunction-associated steatotic liver disease (MASLD) and steatohepatitis (NASH)-associated liver cancer. The authors aim to highlight recent omics research on novel genetic and protein contributions to NAFLD/NASH and discuss mainstream molecular pathways and potential biomarkers and molecular targets. Special attention is given to the role of mTOR in the progression from NASH to hepatocellular carcinoma (HCC).
Meeting Abstract
Oncology
Masaki Fujioka, Min Gi, Shugo Suzuki, Yuji Oishi, Guiyu Qiu, Anna Kakehashi, Hideki Wanibuchi
Article
Pathology
Shugo Suzuki, Kazuhisa Asai, Min Gi, Kazuya Kojima, Anna Kakehashi, Yuji Oishi, Taisuke Matsue, Nao Yukimatsu, Kazuto Hirata, Tomoya Kawaguchi, Hideki Wanibuchi
Summary: This study evaluated the effects of short-term exposure to cigarette smoke in mice. The results showed that cigarette smoke caused inflammatory responses, increased the number of inflammatory cells, and affected the bronchial epithelium. Furthermore, potential markers of the toxic effects of cigarette smoke exposure were identified.
JOURNAL OF TOXICOLOGIC PATHOLOGY
(2022)
Meeting Abstract
Oncology
Shugo Suzuki, Min Gi, Masaki Fujioka, Anna Kakehashi, Hideki Wanibuchi
Meeting Abstract
Oncology
Anna Kakehashi, Shugo Suzuki, Masaki Fujioka, Min Gi, Hideki Wanibuchi
Meeting Abstract
Oncology
Masaki Fujioka, Min Gi, Shugo Suzuki, Yuji Oishi, Takashi Yamaguchi, Taisuke Matsue, Anna Kakehashi, Hideki Wanibuchi
Meeting Abstract
Oncology
Min Gi, Shugo Suzuki, Masaki Fujioka, Anna Kakehashi, Hideki Wanibuchi
Meeting Abstract
Oncology
Anna Kakehashi, Shugo Suzuki, Min Gi, Hideki Wanibuchi
Meeting Abstract
Oncology
Min Gi, Shugo Suzuki, Anna Kakehashi, Taisuke Matsue, Hideki Wanibuchi
