Review
Horticulture
Zhanbin Sun, Shufan Yu, Yafeng Hu, Yanchen Wen
Summary: This review provides an overview of the biocontrol strategies for cucumber downy mildew (CDM). It introduces the biological characteristics of Pseudoperonospora cubensis and recommends screening strategies for biocontrol agents. The review also summarizes the current biocontrol agents with the ability to control CDM, discusses their control characteristics and potential mechanisms, and provides suggestions for future research.
Article
Plant Sciences
Dennis N. Katuuramu, Sandra E. Branham, Amnon Levi, W. Patrick Wechter
Summary: C. amarus germplasm collection shows wide phenotypic variability for resistance to Cucurbit downy mildew (CDM), providing potential candidate genes for genomics-assisted breeding of watermelon with improved CDM resistance.
Article
Plant Sciences
Dor Barnea, Uri Yermiyahu, Dalia Rav-David, Yigal Elad
Summary: It was found that increasing nitrogen concentrations in irrigation water increased the severity of cucumber downy mildew, while increasing potassium or calcium concentrations gradually increased the severity. On the other hand, increasing phosphorus and magnesium concentrations in irrigation water decreased the severity. Spraying salts with chloride and sulfate anions suppressed the disease in most cases, and there was a negative relationship between salt and anion concentrations and disease severity. Spraying with specific salts and monopotassium phosphate reduced downy mildew under commercial conditions.
Article
Plant Sciences
Sharifa G. Crandall, Marina L. Ramon, Alyssa K. Burkhardt, Julian Camilo Bello Rodriguez, Nanci Adair, David H. Gent, Mary K. Hausbeck, Lina M. Quesada-Ocampo, Frank N. Martin
Summary: The study developed a highly specific multiplex TaqMan PCR assay that can accurately detect and quantify three aerially dispersed plant pathogens in a single amplification, improving management options for controlling the diseases they cause.
Article
Microbiology
Marcin Nowicki, Denita Hadziabdic, Robert N. Trigiano, Sarah L. Boggess, Loukas Kanetis, Phillip A. Wadl, Peter S. Ojiambo, Marc A. Cubeta, Otmar Spring, Marco Thines, Fabian Runge, Brian E. Scheffler
Summary: This study used microsatellites to analyze the genetic diversity and spatial distribution of P. cubensis and P. humuli, revealing the genome-scale organizational relationship between the two pathogens. The results provided strong evidence supporting the hypothesis that they are distinct species, with potential further speciation within the P. cubensis complex.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agronomy
Sean M. Toporek, Sandra E. Branham, Melanie L. Katawczik, Anthony P. Keinath, W. Patrick Wechter
Summary: This study identified QTLs underlying resistance to Pseudoperonospora cubensis in Cucumis melo for the first time using a genetically characterized isolate. Nine QTLs associated with resistance were discovered, with two major QTLs identified. One major QTL, qPcub-10.1, co-located with a gene known for providing resistance to other diseases in plants, suggesting its potential importance in cucurbit downy mildew resistance.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Plant Sciences
Isaack Kikway, Anthony P. Keinath, Peter S. Ojiambo
Summary: Cucurbit downy mildew, caused by Pseudoperonospora cubensis, is a significant disease for cucurbits worldwide. Alternating fungicides with different modes of action is recommended to prevent the development of resistance. This study investigated the shifts in sensitivity profiles of P. cubensis isolates during the growing season in response to various fungicides.
Article
Plant Sciences
Isaack Kikway, Anthony P. Keinath, Peter S. Ojiambo
Summary: This study investigated the occurrence of oospores in naturally infected cucurbit leaves in North Carolina and South Carolina, and determined the viability and survival of oospores in outdoor conditions. The study found that oospore viability decreased over time, with factors like soil temperature and number of rainy days affecting the decline.
Article
Plant Sciences
Nagaraju Jhansirani, Venkatappa Devappa, Chittarada Gopal Sangeetha, Shankarappa Sridhara, Kodegandlu Subbanna Shankarappa, Mooventhiran Mohanraj
Summary: Through homology modeling and docking analysis, it was found that phytochemicals from cucumber, antimicrobial compounds from botanical sources, and chemically synthesized compounds can interact with proteins linked to cucumber downy mildew, showing potential antifungal activity.
Article
Agronomy
Ritu Rani, Priyanka Negi, Shikha Sharma, Sandeep Jain
Summary: Downy mildew disease is a major constraint to sustainable cucurbit production in India. This study provides the first morphological and molecular evidence of the occurrence of oospores of P. cubensis in India. These findings suggest that oospores can survive as an overwintering stage under Indian conditions and play an important role in the causation of the disease.
Article
Plant Sciences
Julian C. Bello, Monique L. Sakalidis, David E. Perla, Mary K. Hausbeck
Summary: This study improved the detection system for the pathogen causing cucurbit downy mildew in Michigan by using molecular biology methods. The qPCR assay allowed for rapid and accurate identification of different types of downy mildew pathogens in field samples.
Article
Plant Sciences
A. Rahman, J. R. Standish, K. N. D'Arcangelo, L. M. Quesada-Ocampo
Summary: Pseudoperonospora cubensis is the cause of cucurbit downy mildew, with annual epidemics in the United States due to windborne sporangia traveling long distances and surviving prolonged exposure to sunlight. A multiplex quantitative PCR assay has been developed to detect the pathogen early on and improve disease management.
Article
Biochemistry & Molecular Biology
Bin Wang, Zhaolin Xue, Jie Lan, Mingyou Sun, Qin Sun, Zhongqiao Huang, Can Zhang, Xili Liu
Summary: Y18501 is a new oxysterol-binding protein inhibitor that has similar structure to oxathiapiprolin. It exhibits strong inhibitory activities against Phytophthora spp. and Pseudoperonospora cubensis, with EC50 ranging from 0.0005 to 0.0046 μg/mL. Y18501 has good control efficacy on cucumber downy mildew, effectively inhibits different development stages of P. cubensis, and shows excellent protective and curative activities against the pathogen. It also has acropetal systemic mobility in cucumber leaves and can be taken up and translocated more effectively from the lower leaves to the upper leaves compared to from the roots. The simultaneous application of Y18501 and chlorothalonil can significantly enhance the inhibition of P. cubensis.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agronomy
Osama O. Atallah, Ali Osman, Mohamed A. S. Ali, Mahmoud Sitohy
Summary: The study showed that soybean beta-conglycinin and catfish p22 glycoproteins have significant antifungal activity against P. cubensis, comparable to highly efficient chemical fungicides. Chickpea vicilin and duck egg ovomucin were less efficient. All tested glycoproteins provided full protection for newly emerged cucumber leaves.
PEST MANAGEMENT SCIENCE
(2021)
Article
Plant Sciences
Mehmet Metin Ozguven, Ziya Altas
Summary: This study used image processing algorithms to diagnose mildew disease in cucumber plants, and compared the results with expert assessments. The image processing method showed a significant positive relationship with expert assessments, indicating that it can replace expert assessments.
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Microbiology
Kyriaki Glynou, Tahir Ali, Ann-Katrin Buch, Sevda Haghi Kia, Sebastian Ploch, Xiaojuan Xia, Ali Celik, Marco Thines, Jose G. Macia-Vicente
ENVIRONMENTAL MICROBIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Young-Joon Choi, Gordon Beakes, Sally Glockling, Julia Kruse, Bora Nam, Lisa Nigrelli, Sebastian Ploch, Hyeon-Dong Shin, Roger G. Shivas, Sabine Telle, Hermann Voglmayr, Marco Thines
MOLECULAR ECOLOGY RESOURCES
(2015)
Article
Mycology
Irina Solovyeva, Angelika Schmuker, Liliana M. Cano, Mireille van Damme, Sebastian Ploch, Sophien Kamoun, Marco Thines
MYCOLOGICAL PROGRESS
(2015)
Article
Biochemistry & Molecular Biology
Zakaria Cheikh-Ali, Kyriaki Glynou, Tahir Ali, Sebastian Ploch, Marcel Kaiser, Marco Thines, Helge B. Bode, Jose G. Macia-Vicente
Article
Plant Sciences
Tahir Ali, Fabian Runge, Ayan Dutbayev, Angelika Schmuker, Irina Solovyeva, Lisa Nigrelli, Ann-Katrin Buch, Xiaojuan Xia, Sebastian Ploch, Ouria Orren, Volker Kummer, Juraj Paule, Ali Celik, Ljudmila Vakhrusheva, Ivan Gabrielyan, Marco Thines
Article
Microbiology
Sebastian Ploch, Laura E. Rose, David Bass, Michael Bonkowski
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2016)
Article
Plant Sciences
Tahir Ali, Angelika Schmuker, Fabian Runge, Irina Solovyeva, Lisa Nigrelli, Juraj Paule, Ann-Katrin Buch, Xiaojuan Xia, Sebastian Ploch, Ouria Orren, Volker Kummer, Ib Linde-Laursen, Marian Orgaard, Thure Pablo Hauser, Ali Celik, Marco Thines
Article
Microbiology
Melanie Sapp, Sebastian Ploch, Anna M. Fiore-Donno, Michael Bonkowski, Laura E. Rose
ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Mycology
Marlena Goerg, Sebastian Ploch, Julia Kruse, Volker Kummer, Fabian Runge, Young-Joon Choi, Marco Thines
MYCOLOGICAL PROGRESS
(2017)
Article
Evolutionary Biology
Sebastian Ploch, Young-Joon Choi, Marco Thines
ORGANISMS DIVERSITY & EVOLUTION
(2018)
Editorial Material
Plant Sciences
Mohammad Reza Mirzaee, Sebastian Ploch, Lisa Nigrelli, Sepide Sajedi, Marco Thines
JOURNAL OF PLANT PATHOLOGY
(2018)
Article
Mycology
Ditte Bandini, Bernd Oertel, Sebastian Ploch, Tahir Ali, Jukka Vauras, Anja Schneider, Markus Scholler, Ursula Eberhardt, Marco Thines
MYCOLOGICAL PROGRESS
(2019)
Article
Forestry
Bagdevi Mishra, Bartosz Ulaszewski, Sebastian Ploch, Jaroslaw Burczyk, Marco Thines
Summary: Chloroplast assembly is challenging due to large inverted repeats, but accurate assemblies are important for biogeography and population genetics studies. A study on European Beech chloroplast genomes revealed an inverted orientation of the single-copy region and potential loci for future SNP-based studies. The low divergence suggests limited seed dispersal but high pollen dispersal, with implications for tracing migration history in the Holocene.
Article
Ecology
Thure P. Hauser, Stina Christensen, Vera Kuzina, Marco Thines, Sebastian Ploch, Soren Bak
Summary: Plants have evolved various defenses against herbivores and pathogens, but the effectiveness of these defenses can be influenced by multiple factors. Research on Barbarea vulgaris revealed that different resistance mechanisms have independent evolution and effects, with Albugo resistance benefiting plants while insect resistance showed limited benefits under certain conditions.
ARTHROPOD-PLANT INTERACTIONS
(2021)