Article
Medicine, Research & Experimental
Lee-Moay Lim, Wen-Yu Chung, Daw-Yang Hwang, Chih-Chuan Yu, Hung-Lung Ke, Peir-In Liang, Ting-Wei Lin, Siao Muk Cheng, A-Mei Huang, Hung-Tien Kuo
Summary: This study compares the genetic alterations in urothelial carcinoma (UC) developed after kidney transplantation (UCKT) and in UC in patients on hemodialysis (UCHD). Specific gene mutations were found in UCKT, potentially contributing to the understanding of the molecular pathogenesis of urothelial carcinoma.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Xingchen Fan, Xuan Zou, Cheng Liu, Shuang Peng, Shiyu Zhang, Xin Zhou, Jun Zhu, Wei Zhu
Summary: This study aimed to identify critical miRNA-mRNA regulation pairs contributing to lung adenocarcinoma (LUAD) pathogenesis. Through analysis of miRNA and mRNA expression data, potential regulation pairs were identified and their diagnostic value and biological functions were confirmed. The study also analyzed the expression and clinical characteristics of these regulation pairs under different conditions.
Review
Biochemistry & Molecular Biology
Marco Fiorentino, Francesca Bagagli, Annamaria Deleonardis, Alessandra Stasi, Rossana Franzin, Francesca Conserva, Barbara Infante, Giovanni Stallone, Paola Pontrelli, Loreto Gesualdo
Summary: Kidney transplantation is the preferred treatment for end-stage renal disease (ESRD). Kidney transplant recipients (KTRs) are at a higher risk of ICU admission, mainly in the late post-transplant period. Urosepsis and bloodstream infections are the main reasons for ICU admissions, and side effects of immunosuppressive treatment should also be accounted for. AKI incidence is common in KTRs during ICU stay, and in-hospital mortality can be high. The present review aims to explore the impact of AKI on outcomes in KTRs and provide insights into its diagnosis, management, and immunosuppression modulation.
Article
Genetics & Heredity
Duan Guo, Yu Fan, Ji-Rong Yue, Tao Lin
Summary: By constructing a miRNA-mRNA network, the study revealed the molecular mechanisms of post-Tx AKI, highlighting the key roles of miRNA and mRNA in kidney-related functions and various signaling pathways.
Article
Biochemistry & Molecular Biology
Weiyang Fan, Rui Shi, Minyi Guan, Pan Chen, Hao Wu, Weiwei Su, Yonggang Wang, Peibo Li
Summary: This study unveiled the regulatory mechanism of naringenin in HepaRG cells through integrated analysis of mRNA-seq and miRNA-seq, identifying multiple miRNA-mRNA pairs involved in liver metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Ming Wang, Yan Cui, Yang Cai, Yugang Jiang, Yong Peng
Summary: This study identified six hub genes and two regulatory pathways related to LGG through comprehensive analysis of mRNA-miRNA networks, providing important clues for understanding the mechanisms underlying the development of LGG.
CANCER MANAGEMENT AND RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ming Zhu, Qianshi Dong, Jie Bing, Songbuerbatu, Lamei Zheng, Tashi Dorjee, Qi Liu, Yijun Zhou, Fei Gao
Summary: Long non-coding RNAs (lncRNAs) play critical roles in the cold stress response of Ammopiptanthus nanus. A combined analysis identified 4890 novel lncRNAs, of which 1322 showed differential expression under cold stress. These lncRNAs formed modules to regulate stress-related genes, including transcription factors and enzymes. Additionally, lncRNAs also participated in the cold stress response through miRNA-mediated regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Bowen Chen, Yaojing Yue, Jianye Li, Chao Yuan, Tingting Guo, Dan Zhang, Jianbin Liu, Bohui Yang, Zengkui Lu
Summary: This study investigated the methylation patterns and differences in miRNA and mRNA expression in the F1 sheep longissimus dorsi muscle tissue using RNA sequencing, bisulfite sequencing, and small-RNA sequencing. The results identified differentially expressed genes, differentially methylated regions, and differentially expressed miRNAs that are significantly enriched in AMPK signaling, fatty acid degradation, metabolism, and other related pathways. Furthermore, a DNA methylation-mRNA and miRNA-mRNA coexpression network was constructed. The study provides valuable information and evidence for understanding the regulation of muscle growth and fat metabolism in hybrid sheep populations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Engineering, Biomedical
Chenguang Ding, Bo Wang, Xiang Feng Lai, Yingcong Guo, Greg Tesch, Xiaoming Ding, Jin Zheng, PuXun Tian, Sharon Ricardo, Hsin-Hui Shen, Wujun Xue
Summary: Renal fibrosis, a common manifestation of chronic kidney disease, can be suppressed by inducing local production of relaxin-2 mRNA in kidney cells. In both in vitro and in vivo experiments, delivery of relaxin-2 mRNA reduced fibrotic gene expression and cell proliferation, inhibiting the development of renal fibrosis. The use of cubosomes as delivery vehicles for relaxin-2 mRNA also alleviated fibrosis and inflammation in a mouse model of renal injury. Therefore, relaxin-2 mRNA shows potential as a novel therapy for inhibiting fibrosis and inflammation in chronic kidney disease.
MATERIALS TODAY BIO
(2023)
Article
Plant Sciences
Daojin Sun, Jing Zhang, Jun He, Zhiqiang Geng, Song Li, Jiali Zhang, Peiling Li, Lingling Zhang, Zhenxing Wang, Likai Wang, Fadi Chen, Aiping Song
Summary: In this study, the genome annotation of C. seticuspe was improved, and the expression profiles of flower development-related genes, ceRNA networks, and lncNAT networks were identified, providing a foundation for understanding the regulatory mechanisms underlying disc floret and ray floret formation.
Article
Multidisciplinary Sciences
David Andrijevic, Zvonimir Vrselja, Taras Lysyy, Shupei Zhang, Mario Skarica, Ana Spajic, David Dellal, Stephanie L. Thorn, Robert B. Duckrow, Shaojie Ma, Phan Q. Duy, Atagun U. Isiktas, Dan Liang, Mingfeng Li, Suel-Kee Kim, Stefano G. Daniele, Khadija Banu, Sudhir Perincheri, Madhav C. Menon, Anita Huttner, Kevin N. Sheth, Kevin T. Gobeske, Gregory T. Tietjen, Hitten P. Zaveri, Stephen R. Latham, Albert J. Sinusas, Nenad Sestan
Summary: Researchers have developed a technology called OrganEx that can maintain tissue integrity, reduce cell death, and restore molecular and cellular processes in multiple vital organs in a pig's whole body. Single-nucleus transcriptomic analysis revealed gene expression patterns specific to organ and cell type, reflecting specific molecular and cellular repair processes. This study reveals the underappreciated potential for cellular recovery after prolonged whole-body warm ischemia in large mammals.
Review
Medicine, General & Internal
Huawei Cao, Jiandong Zhang, Zejia Sun, Jiyue Wu, Changzhen Hao, Wei Wang
Summary: With the rapid aging of the global population, frailty has become a widely addressed problem by researchers in medical fields. The high prevalence of frailty among kidney transplant candidates and recipients has attracted attention. However, current studies mainly focus on the incidence and relationship of frailty in this population, with limited research on the pathogenesis and intervention. Exploring the pathogenesis of frailty and determining effective intervention measures may improve the outcomes for kidney transplant recipients.
CHINESE MEDICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Guanzhong Chen, Bowen Liu, Shiqun Chen, Huanqiang Li, Jin Liu, Ziling Mai, Enzhao Chen, Chunyun Zhou, Guoli Sun, Zhaodong Guo, Li Lei, Shanyi Huang, Liyao Zhang, Min Li, Ning Tan, Hong Li, Yulin Liao, Jia Liu, Jiyan Chen, Yong Liu
Summary: This study identified 357 differentially expressed lncRNAs in PC-AKI, with lnc-HILPDA and lnc-PRND emerging as potential early detection biomarkers in both rat models and clinical samples. These lncRNAs showed promising accuracy in distinguishing PC-AKI patients, with a high sensitivity and specificity. Time-course experiments revealed the optimal timing for detecting these lncRNAs in PC-AKI rats, highlighting their clinical implications for early detection and stratification of PC-AKI.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Taohua Wei, Nannan Qian, Wenming Yang, Yue Yang, Jie Liu, Wenjie Hao, Ting Cheng, Ran Yang, Wei Dong, Yulong Yang
Summary: This study identified the circRNA/miRNA/mRNA network involved in kidney failure in WD and demonstrated its potential as a biomarker for the pathogenesis of WD.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Lianwei Ma, Hui Zhang, Yue Zhang, Hailong Li, Minghui An, Bin Zhao, Haibo Ding, Junjie Xu, Hong Shang, Xiaoxu Han
Summary: This study identified a significant number of differentially expressed lncRNAs, mRNAs, and miRNAs in HAART-naive EHI patients compared to healthy controls. The findings suggest that lncRNAs may play important roles in HIV-1 replication and immune activation during early infection, providing insights for antiviral therapy.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Endocrinology & Metabolism
Hiddo J. L. Heerspink, Paul Perco, Skander Mulder, Johannes Leierer, Michael K. Hansen, Andreas Heinzel, Gert Mayer
Article
Multidisciplinary Sciences
Julia Wilflingseder, Michaela Willi, Hye Kyung Lee, Hannes Olauson, Jakub Jankowski, Takaharu Ichimura, Reinhold Erben, M. Todd Valerius, Lothar Hennighausen, Joseph Bonventre
NATURE COMMUNICATIONS
(2020)
Article
Surgery
Gabor Telkes, Laszlo Piros, Jozsef Szabo, Gergely Huszty, Katalin Eitler, Laszlo Kobori
Summary: Third kidney transplantations are more complex and time-consuming, with higher rates of delayed graft function and inferior patient and graft survival compared to first transplantations. Careful selection is necessary to minimize risks and improve outcomes.
LANGENBECKS ARCHIVES OF SURGERY
(2021)
Review
Endocrinology & Metabolism
Sara Denicolo, Paul Perco, Stefanie Thoeni, Gert Mayer
Summary: Cardiovascular and renal complications are a major burden for individuals with type 2 diabetes mellitus. Non-adherence to medication is common and associated with worse outcomes and higher healthcare costs in chronic conditions like T2DM. Adherence to drug therapy is crucial for successful disease management and prevention of comorbidities.
JOURNAL OF DIABETES AND ITS COMPLICATIONS
(2021)
Article
Surgery
K. Eitler, Z. Mathe, V. Papp, A. Zalatnai, A. Bibok, P. A. Deak, L. Kobori, G. Telkes
Summary: This case involves a 68-year-old male patient with situs inversus totalis and obstructive jaundice caused by a malignant common bile duct stricture. Despite a series of diagnostic and therapeutic procedures, the final pathology did not confirm malignancy. Situs inversus patients present unique challenges in both diagnostic and therapeutic aspects, with benign biliary strictures often misdiagnosed as cancer.
Article
Biochemistry & Molecular Biology
Li Li, Pierre Galichon, Xiaoyan Xiao, Ana C. Figueroa-Ramirez, Diana Tamayo, Jake J-K Lee, Marian Kalocsay, David Gonzalez-Sanchez, Maria S. Chancay, Kyle W. McCracken, Nathan N. Lee, Takaharu Ichimura, Yutaro Mori, M. Todd Valerius, Julia Wilflingseder, Dario R. Lemos, Elazer R. Edelman, Joseph Bonventre
Summary: COUP-TFII plays a crucial role in fibrosis, with its overexpression suppressing fatty acid oxidation, enhancing glycolysis pathways, and inducing fibroblasts to produce collagen. Genetic ablation of COUP-TFII can mitigate kidney fibrosis after injury. The regulation of PGC1α by COUP-TFII serves as an important mechanism in fibrosis.
Article
Biochemistry & Molecular Biology
Johannes Leierer, Paul Perco, Benedikt Hofer, Susanne Eder, Alexander Dzien, Julia Kerschbaum, Michael Rudnicki, Gert Mayer
Summary: Changes in protein concentrations in the body fluids of ADPKD patients mainly involve dysregulation of various molecular processes, including markers such as EGF, APLN, VEGFA, and AGT. These markers are significantly correlated with renal function and disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Maria Effenberger, Andreas Kronbichler, Erica Bettac, Felix Grabherr, Christoph Grander, Timon Erik Adolph, Gert Mayer, Heinz Zoller, Paul Perco, Herbert Tilg
Summary: Liver transplantation (LT) is the only curative treatment for end-stage liver disease, but public awareness has significantly decreased over the past two decades. Novel awareness programs are needed to address this issue and increase donor acquisition.
JOURNAL OF MEDICAL INTERNET RESEARCH
(2021)
Article
Multidisciplinary Sciences
Jakub Jankowski, Hye Kyung Lee, Julia Wilflingseder, Lothar Hennighausen
Summary: This study reports on the genetic programs activated in kidney cells in response to interferons during COVID-19, and the inhibitory effect of a Janus kinase inhibitor. The findings help identify kidney-specific interferon responses.
Article
Biochemistry & Molecular Biology
Michail Evgeniou, Juan Manuel Sacnun, Klaus Kratochwill, Paul Perco
Summary: This study conducted a meta-analysis of transcriptomics datasets in the context of peritoneal dialysis, identifying dysregulated receptor-ligand interactions associated with PD complications. The study revealed novel receptor-ligand interactions that warrant further investigation in future functional studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Urology & Nephrology
Sara Denicolo, Verena Vogi, Felix Keller, Stefanie Thoni, Susanne Eder, Hiddo J. L. Heerspink, Laszlo Rosivall, Andrzej Wiecek, Patrick B. Mark, Paul Perco, Johannes Leierer, Andreas Kronbichler, Marion Steger, Simon Schwendinger, Johannes Zschocke, Gert Mayer, Emina Jukic
Summary: This study investigates the impact of clonal hematopoiesis of indeterminate potential (CHIP) on the incidence and progression of diabetic kidney disease (DKD). The results show that CHIP is not associated with the development or progression of DKD, while other known risk factors and elevated microinflammation are related to kidney function decline in type 2 diabetes mellitus.
KIDNEY INTERNATIONAL REPORTS
(2022)
Article
Medicine, Research & Experimental
Glory Ranches, Maximilian Zeidler, Roman Kessler, Martina Hoelzl, Michael W. Hess, Jonathan Vosper, Paul Perco, Herbert Schramek, Kai K. Kummer, Michaela Kress, Anne Krogsdam, Michael Rudnicki, Gert Mayer, Alexander Huettenhofer
Summary: Exosomes are valuable biomarkers for studying chronic kidney disease (CKD). Urinary exosomes and exosomes derived from renal proximal tubular epithelial cells (RPTECs) were analyzed for microRNA (miRNA) profiles. Dysregulated exosomal miRNAs associated with pro-inflammatory and pro-fibrotic pathways were identified in RPTECs, suggesting their potential use in predicting CKD pathogenesis.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Editorial Material
Urology & Nephrology
Paul Perco, Gert Mayer
Summary: With the advancement of single-cell sequencing techniques and bioinformatic analysis, complex physiology and pathophysiology of cell-cell interactions in the kidney can be better understood, leading to the identification of new biomarkers and treatment options.
KIDNEY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Sara Denicolo, Viji Nair, Johannes Leierer, Michael Rudnicki, Matthias Kretzler, Gert Mayer, Wenjun Ju, Paul Perco
Summary: The study found that Fibrinogen-like 2 (FGL2) is associated with fibrosis in kidney damage and may be a potential therapeutic target for chronic kidney disease (CKD). Analysis of CKD cohorts showed that higher renal FGL2 mRNA expression is associated with kidney fibrosis and worse outcomes. FGL2 protein expression was detected in specific renal tissue sections. The study also identified regulators of FGL2 expression in renal tissue, as well as factors potentially inducing or counterbalancing FGL2-co-expressed genes. In conclusion, elevated FGL2 levels in human CKD are associated with fibrosis and worse outcomes.
Article
Medical Laboratory Technology
Christoph A. Gebeshuber, Lisa Daniel-Fischer, Heinz Regele, Helga Schachner, Christoph Aufricht, Christoph Kornauth, Matthias Ley, Seth L. Alper, Rebecca Herzog, Klaus Kratochwill, Paul Perco
Summary: Focal segmental glomerulosclerosis (FSGS) is a glomerular lesion associated with nephrotic syndrome and a high risk of end-stage kidney disease. Current treatment options are limited, and there is a need for novel therapies targeting specific molecular pathways. Using a network-based molecular model, the anti-platelet drug clopidogrel was identified as a potential therapeutic option for FSGS. Experimental validation in a mouse model showed that clopidogrel improved FSGS outcome parameters and histopathological damage. Its favorable safety profile and efficacy recommend clopidogrel for clinical trials in FSGS.
TRANSLATIONAL RESEARCH
(2023)