Article
Oncology
Jian Lin, Yuting Dai, Chen Sang, Guohe Song, Bin Xiang, Mao Zhang, Liangqing Dong, Xiaoli Xia, Jiaqiang Ma, Xia Shen, Shuyi Ji, Shu Zhang, Mingjie Wang, Hai Fang, Xiaoming Zhang, Xiangdong Wang, Bing Zhang, Jian Zhou, Jia Fan, Hu Zhou, Daming Gao, Qiang Gao
Summary: This study reveals the heterogeneity of the immunogenomic traits in intrahepatic cholangiocarcinoma (iCCA) among patients, which poses challenges and opportunities for personalized immunotherapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Immunology
Laty G. Thiam, Prince B. Nyarko, Felix Ansah, Makhtar Niang, Gordon A. Awandare, Yaw Aniweh
Summary: This study reveals a low level of phenotypic diversity in Ghanaian clinical isolates of P. falciparum, with a predominant SA-independent invasion pathway and an important role for the PfRh4-CR1 mediated alternative pathway. Additionally, there is little variation in gene expression profiles within and between parasite populations across different malaria endemic areas. These findings have implications for the development of new intervention strategies, such as blood-stage malaria vaccine targets.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Environmental
Xing Liu, Ji Zhan, Lu Liu, Feiting Gan, Jie Ye, Kenneth H. Nealson, Christopher Rensing, Shungui Zhou
Summary: This study introduces an in situ anaerobic spectroelectrochemical coculture cell (ASCC) to induce the formation of direct interspecies electron transfer (DIET) and characterize it. Through observing two typical Geobacter DIET cocultures, it was found that cytochromes are abundant in DIET coculture, indicating a cytochrome-mediated electron transfer mechanism.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Mengge Li, Min Du, Hui Cong, Yuanyuan Gu, Yuan Fang, Jin Li, Yu Gan, Hong Tu, Jinyang Gu, Qiang Xia
Summary: This study revealed that HBV integration is a common event in HBV-related ICC and identified several recurrent HBV integration genes, such as TERT, which provide new clues for further exploring the relationship between HBV infection and ICC.
HEPATOLOGY RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Lingqi Yu, Jun Yan, Yingqi Zhan, Anyao Li, Lidan Zhu, Jingyang Qian, Fanfan Zhou, Xiaoyan Lu, Xiaohui Fan
Summary: This study used single-cell RNA sequencing to investigate the dynamics and functions of hepatic non-parenchymal cells (NPCs) in autoprotection against drug-induced liver injury (DILI). The findings revealed the important roles of specific cell subsets, including thorn endothelial cells, dendritic cells, natural killer cells, and anti-inflammatory macrophages and neutrophils, in autoprotection against DILI. These findings provide new insights into the mechanisms of DILI and may contribute to the development of prevention and therapy strategies.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Emmanuel N. Paul, Gregory W. Burns, Tyler J. Carpenter, Joshua A. Grey, Asgerally T. Fazleabas, Jose M. Teixeira
Summary: Transcriptome analysis revealed significant phenotypic and gene expression differences between fibroid-associated myometrium and non-diseased myometrium, particularly in upregulated genes related to extracellular matrix and structure organization. These results suggest that fibroid studies should consider using both matched myometrium and non-diseased myometrium as controls to better understand the pathophysiology of uterine fibroids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Linda B. B. Baughn, Erik Jessen, Neeraj Sharma, Hongwei Tang, James B. B. Smadbeck, Mark D. D. Long, Kathryn Pearce, Matthew Smith, Surendra Dasari, Zohar Sachs, Michael A. A. Linden, Joselle Cook, A. Keith Stewart, Marta Chesi, Amit Mitra, P. Leif Bergsagel, Brian Van Ness, Shaji K. K. Kumar
Summary: This study used mass cytometry to analyze 49 primary tumor samples from multiple myeloma patients and identified 13 phenotypic clusters. The abundance of one of these clusters was found to be associated with treatment response and overall survival.
BLOOD CANCER JOURNAL
(2023)
Article
Cell Biology
Yuichi Yoshida, Sachiyo Yoshio, Taiji Yamazoe, Taizo Mori, Yuriko Tsustui, Hironari Kawai, Shiori Yoshikawa, Takasuke Fukuhara, Toru Okamoto, Yoshihiro Ono, Yu Takahashi, Ryuki Hashida, Takumi Kawaguchi, Akinobu Taketomi, Tatsuya Kanto
Summary: Overall response rates to systemic therapies against advanced hepatocellular carcinoma are not satisfactory, prompting the search for new immunotherapy targets. NK cells play a crucial role in the tumor microenvironment and their phenotypes in HCC patients suggest potential targets for immunotherapy, including CD160 and CD49a on CD56(dim) NK cells. Further functional analysis of these markers could lead to new immunotherapy strategies for HCC patients.
Article
Plant Sciences
Fekadu Gadissa, Temesgen Bedassa Gudeta
Summary: The research aimed to test the performance of 150 ex-situ conserved Ethiopian barley landraces collected from Arsi and Bale highlands. Results showed good germination rates and varying traits frequencies among the landraces, highlighting the importance of considering environmental effects in genetic selection. High phenotypic coefficients of variation compared to genotypic coefficients suggest significant environmental influence on trait variation. Further testing and molecular marker systems are needed for yield improvement and conservation of Ethiopian barley landraces in the face of current erratic climate change.
Article
Allergy
Carolina Perales-Chorda, David Obeso, Laura Twomey, Ayelen Rojas-Benedicto, Leonor Puchades-Carrasco, Marta Roca, Antonio Pineda-Lucena, Jose Julio Laguna, Coral Barbas, Vanesa Esteban, Jaume Marti-Garrido, Ethel Ibanez-Echevarria, Ramon Lopez-Salgueiro, Domingo Barber, Alma Villasenor, Dolores Hernandez Fernandez de Rojas
Summary: This study aimed to explore metabolic changes in patients suffering from anaphylactic reactions based on triggers (food and/or drug) and severity (moderate and severe). Results indicate that different triggers or severity levels can induce differential metabolic changes at different time points, offering new insights into understanding, diagnosing, and treating anaphylaxis.
CLINICAL AND EXPERIMENTAL ALLERGY
(2021)
Article
Microbiology
Mariana Neri Lucas Kurihara, Ingrid Nayara Marcelino Santos, Ana Karolina Antunes Eisen, Giovana Santos Caleiro, Jansen de Araujo, Romario Oliveira de Sales, Antonio Carlos Pignatari, Mauro Jose Salles
Summary: This study investigated the frequency of C. acnes identification in deep tissue specimens of patients undergoing clean shoulder surgery and evaluated its phenotypic and genetic traits associated with virulence and antibiotic resistance patterns. The presence of polyclonal C. acnes in multiple deep tissue samples does not necessarily indicate infection.
Article
Biochemistry & Molecular Biology
Susann Stephan-Falkenau, Anna Streubel, Thomas Mairinger, Torsten-Gerriet Blum, Jens Kollmeier, Fabian D. Mairinger, Torsten Bauer, Joachim Pfannschmidt, Manuel Hollmann, Michael Wessolly
Summary: Pulmonary sarcomatoid carcinoma (PSC) is a highly aggressive subtype of lung cancer with poor clinical outcomes. In this study, we characterized 179 PSC cases and identified clinical, immunological, and molecular features using various techniques. We found that PSC is more common in men, older individuals, and smokers. Surgery was associated with improved overall survival. PD-L1 expression was detected in the majority of patients, and altered epitopes due to processing mutations contributed to immune escape and decreased survival. Genomic alterations for which there are approved drugs were present in a subset of patients. MET exon 14 skipping was found to be more frequent compared to other non-small cell lung cancers. In conclusion, the unique genomic and immunological features of PSC should be considered in therapy stratification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ecology
Kevin W. Becker, Matthew J. Harke, Daniel R. Mende, Daniel Muratore, Joshua S. Weitz, Edward F. DeLong, Sonya T. Dyhrman, Benjamin A. S. Van Mooy
Summary: The study revealed that in surface waters of the North Pacific Subtropical Gyre, phytoplankton activity in photosynthesis, photoacclimation, and photoprotection displayed clear diel coordination, with pigment abundances showing a balance between synthesis and consumption. Gene expression exhibited synchronized diel patterns among all taxa, illustrating the significant impact of light on photosynthetic organisms in the ocean.
Article
Multidisciplinary Sciences
Abera Abdeta, Adane Bitew, Surafel Fentaw, Estifanos Tsige, Dawit Assefa, Tadesse Lejisa, Yordanos Kefyalew, Eyasu Tigabu, Martin Evans
Summary: The study detected a high prevalence of multidrug-resistant and extremely drug-resistant carbapenemase-producing gram-negative bacteria, particularly among intensive care unit patients at healthcare facilities. This highlights the increasing difficulty in clinical management of infected patients and the need for last-resort antibiotics, potentially worsening the global antimicrobial resistance crisis. Robust antimicrobial resistance monitoring and infection prevention and control programs are necessary.
Article
Biochemistry & Molecular Biology
Lengxob Yong, Darren P. Croft, Jolyon Troscianko, Indar W. Ramnarine, Alastair J. Wilson
Summary: The study revisits the repeated evolution of brighter males in the Trinidadian guppy, using a multivariate phenotyping approach and ecologically-relevant colour traits. The findings suggest that reduced predation risk may facilitate adaptive differentiation, potentially through female choice, but this proceeds in independent directions of multitrait space across lineages. Pool-sequencing data also indicate the presence of SNPs showing greater differentiation than expected under neutrality, with some located in genes contributing to colour pattern variation, presenting opportunities for future genetic study.
Article
Cell & Tissue Engineering
Emil Bluhme, Ewa Henckel, Roberto Gramignoli, Therese Kjellin, Christina Hammarstedt, Greg Nowak, Ahmad Karadagi, Helene Johansson, Oystein Jynge, Maria Soderstrom, Bjorn Fischler, Stephen Strom, Ewa Ellis, Boubou Hallberg, Carl Jorns
Summary: This study assessed the metabolic function and suitability of hepatocytes isolated from neonatal livers for transplantation. Despite prolonged warm ischemia time, hepatocytes isolated from neonatal donors showed good viability, function, and engraftment. This suggests that neonatal livers should be considered as a donor source for clinical hepatocyte transplantation.
CELL TRANSPLANTATION
(2022)
Article
Immunology
Martin Cornillet, Benedikt Strunz, Olav Rooyackers, Andrea Ponzetta, Puran Chen, Jagadeeswara Rao Muvva, Mira Akber, Marcus Buggert, Benedict J. Chambers, Majda Dzidic, Iva Filipovic, Jean-Baptiste Gorin, Sara Gredmark-Russ, Laura Hertwig, Jonas Klingstrom, Efthymia Kokkinou, Egle Kvedaraite, Magda Lourda, Jenny Mjosberg, Christopher Maucourant, Anna Norrby-Teglund, Tiphaine Parrot, Andre Perez-Potti, Olga Rivera-Ballesteros, Johan K. Sandberg, John Tyler Sandberg, Takuya Sekine, Mattias Svensson, Renata Varnaite, Lars Eriksson, Soo Aleman, Kristoffer Stralin, Hans-Gustaf Ljunggren, Niklas K. Bjorkstrom
Summary: An in-depth assessment of 1129 unique metabolites in 27 hospitalized COVID-19 patients revealed a COVID-19-specific metabolic imprint. Following severe acute respiratory syndrome coronavirus-2 seroconversion, the metabolic response underwent a switch, indicating a network of metabolic trajectories aligned with immune activation pathways and organ damage.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Yanqin Du, Tanvi Khera, Benedikt Strunz, Katja Deterding, Daniel Todt, Norman Woller, Sophie Anna Engelskircher, Svenja Hardtke, Kerstin Port, Andrea Ponzetta, Eike Steinmann, Markus Cornberg, Julia Hengst, Niklas K. Bjorkstrom, Heiner Wedemeyer
Summary: This study comprehensively analyzed the phenotype and reinvigoration capacity of unconventional T cells (UTCs) in acute symptomatic HCV infection. It was found that MAIT cells showed reduced frequency, but remaining cells had near-normal phenotype in acute infection, although they exhibited significant dysfunction upon stimulation that persisted even after viral clearance.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Christine L. Zimmer, Iva Filipovic, Martin Cornillet, Colm J. O'Rourke, Lena Berglin, Hannes Jansson, Dan Sun, Otto Strauss, Laura Hertwig, Helene Johansson, Erik Seth, Ernesto Sparrelid, Joana Dias, Hans Glaumann, Espen Melum, Ewa C. Ellis, Johan K. Sandberg, Jesper B. Andersen, Annika Bergquist, Niklas K. Bjorkstrom
Summary: The study found that MAIT cells were heterogenously lost from intrahepatic and perihilar cholangiocarcinoma tumors possibly in response to increased bacterial burden, while the residual intratumoral MAIT cells exhibited preserved receptor repertoire for interaction with tumor cells. The high presence of MAIT cells in the livers of iCCA patients predicted long-term survival and was associated with a favorable antitumor immune signature.
Article
Gastroenterology & Hepatology
Annika Bergquist, Tobias J. Weismueller, Cynthia Levy, Christian Rupp, Deepak Joshi, Jeremy Shanika Nayagam, Aldo J. Montano-Loza, Ellina Lytvyak, Ewa Wunsch, Piotr Milkiewicz, Roman Zenouzi, Christoph Schramm, Nora Cazzagon, Annarosa Floreani, Ingalill Friis Liby, Miriam Wiestler, Heiner Wedemeyer, Taotao Zhou, Christian P. Strassburg, Eirini Rigopoulou, George Dalekos, Manasa Narasimman, Xavier Verhelst, Helena Degroote, Mette Vesterhus, Andreas E. Kremer, Bennet Buendgens, Fredrik Rorsman, Emma Nilsson, Kristin Kaasen Jorgensen, Erik von Seth, Martin Cornillet Jeannin, Nils Nyhlin, Harry Martin, Stergios Kechagias, Kristine Wiencke, Marten Werner, Benedetta Terziroli Beretta-Piccoli, Marco Marzioni, Helena Isoniemi, Johanna Arola, Agnes Wefer, Jonas Soderling, Martti Farkkila, Henrike Lenzen
Summary: This study compares different follow-up strategies in patients with PSC and finds that scheduled imaging can improve survival. There are considerable variations in follow-up strategies across centers, but multiple factors may contribute to this result.
LIVER INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Andreas Giannisis, Kalicharan Patra, Anna K. Edlund, Lur Agirrezabala Nieto, Joan Benedicto-Gras, Simon Moussaud, Andres de la Rosa, Daniel Twohig, Tore Bengtsson, Yuan Fu, Guojun Bu, Greg Bial, Lander Foquet, Christina Hammarstedt, Stephen Strom, Kristina Kannisto, Jacob Raber, Ewa Ellis, Henrietta M. Nielsen
Summary: Liver-generated plasma apoE, although it does not enter the brain, is correlated with the risk and biomarker levels of Alzheimer's disease (AD). Carriers of APOE ε4, the strongest genetic risk factor for AD, show lower plasma apoE and altered brain integrity even in mid-life. This study investigates the role of liver-derived apoE in promoting neurodegeneration and emphasizes the potential importance of the liver in the APOE ε4-associated risk of neurodegenerative disease.
MOLECULAR PSYCHIATRY
(2022)
Article
Gastroenterology & Hepatology
Christopher L. Bowlus, Lionel Arrive, Annika Bergquist, Mark Deneau, Lisa Forman, Sumera I. Ilyas, Keri E. Lunsford, Mercedes Martinez, Gonzalo Sapisochin, Rachna Shroff, James H. Tabibian, David N. Assis
Article
Immunology
Martha E. Haugstoyl, Martin Cornillet, Kristina Strand, Natalie Stiglund, Dan Sun, Laurence Lawrence-Archer, Iren D. Hjellestad, Ernesto Sparrelid, Christian Busch, Joran Hjelmesaeth, Jens K. Hertel, Andrea Ponzetta, Gunnar Mellgren, Johan Ferno, Niklas K. Bjorkstrom
Summary: Adipose tissue inflammation is a key factor in the development of obesity-related metabolic disorders, and there is emerging evidence suggesting the involvement of adipose tissue T cells in initiating pro-inflammatory signaling. However, there is limited data on human adipose tissue T cells in obesity, mainly due to the lack of specific markers to define tissue-resident T cell subsets. In this study, we used multicolor flow cytometry and RNA sequencing to thoroughly characterize T cells in both blood and adipose tissue, and identified distinct subsets of T cells associated with obesity, expressing activation markers CD26 and CCR5, as well as obesity-specific genes potentially involved in activating pro-inflammatory pathways such as ceramide signaling, autophagy, and IL-6 signaling. These findings enhance our understanding of the heterogeneity of T cells in adipose tissue and shed light on subsets that may contribute to obesity-related pathogenesis.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Review
Pediatrics
Emil Bluhme, Ewa Henckel, Carl Jorns
Summary: Organ transplantation is limited by the scarcity of suitable organs. Neonatal organ donation has the potential to expand the donor pool, but the literature on its prevalence and outcome is fragmented. This review summarizes the available literature on neonatal organ donation and transplantation and highlights successful cases of kidney, heart, liver, hepatocyte, and multivisceral transplantation. While neonatal organs can achieve good posttransplant function, there is an increased risk of thrombosis. Further research is needed to fully explore the potential of neonatal organ donation.
PEDIATRIC TRANSPLANTATION
(2023)
Article
Immunology
Martha E. Haugstoyl, Martin Cornillet, Kristina Strand, Natalie Stiglund, Dan Sun, Laurence Lawrence-Archer, Iren D. Hjellestad, Christian Busch, Gunnar Mellgren, Niklas K. Bjorkstrom, Johan Ferno
Summary: Natural killer (NK) cells play an important role in obesity-related inflammation in adipose tissue. However, the phenotype of NK cell subsets in human adipose tissue and their role in metabolic disorders are poorly understood. This study used multicolor flow cytometry to characterize CD56(bright) and CD56(dim) NK cells in blood and adipose tissue of obese individuals, and identified surface proteins enriched on adipose tissue-resident CD56(bright) NK cells. The study provides valuable insights into the identity of NK cells in blood and adipose tissue in relation to obesity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Martin Cornillet, Helen Zemack, Hannes Jansson, Ernesto Sparrelid, Ewa Ellis, Niklas K. Bjorkstrom
Summary: Alpha-1 antitrypsin deficiency (A1ATD) is present in 10.8% of patients with biliary tract cancer (BTC), with no significant clinical differences compared to non-A1ATD BTC. The abundance of A1AT protein is increased in intrahepatic cholangiocarcinoma (iCCA) and is influenced by genomic alterations. A1ATD may be associated with a less aggressive phenotype, while high A1AT expression may contribute to a more aggressive phenotype.
Article
Endocrinology & Metabolism
Emelie Barreby, Benedikt Strunz, Sebastian Nock, Lea Naudet, Joanne X. Shen, Helene Johansson, Isabella Soennerborg, Junjie Ma, Egon Urgard, Laura J. Pallett, Yizhou Hu, Achilleas Fardellas, Valerio Azzimato, Ana Vankova, Laura Levi, Cecilia Morgantini, Mala K. Maini, Per Stal, Stephan P. Rosshart, Jonathan M. Coquet, Greg Nowak, Erik Naeslund, Volker M. Lauschke, Ewa Ellis, Niklas K. Bjoerkstroem, Ping Chen, Myriam Aouadi
Summary: By studying patients undergoing liver transplantation, a specific population of resident liver myeloid cells that protects against metabolic impairment in obesity is identified. These cells, termed liver myeloid cells 2 (LM2), decrease in proportion during obesity but can ameliorate oxidative stress. These findings suggest that targeting resident myeloid cells could be a therapeutic approach for reducing oxidative stress in non-alcoholic fatty liver disease (NAFLD).
Article
Respiratory System
Laura Palma M. Medina, Haris Babacic, Majda Dzidic, Asa Parke, Marina Garcia, Kimia T. Maleki, Christian Unge, Magda Lourda, Egle Kvedaraite, Puran Chen, Jagadeeswara Rao Muvva, Martin Cornillet, Johanna Emgard, Kirsten Moll, Jakob Michaelsson, Malin Flodstrom-Tullberg, Susanna Brighenti, Marcus Buggert, Jenny Mjosberg, Karl-Johan K. Malmberg, Johan Sandberg, Sara Gredmark-Russ, Olav Rooyackers, Mattias J. Svensson, Benedict I. Chambers, Lars Eriksson, Maria K. Pernemalm, Niklas Bjorkstrom, Soo Aleman, Hans-Gustaf Ljunggren, Jonas Klingstrom, Kristoffer Stralin, Anna Norrby-Teglund
Summary: This study explores the plasma proteome profiles in COVID-19 and sepsis patients, revealing similarities and specific differences. Machine learning identifies biomarkers that accurately differentiate COVID-19 from CAP-sepsis, which has significant implications for personalized management of these diseases.
RESPIRATORY RESEARCH
(2023)
Article
Cell Biology
Quirin Hammer, Angelica Cuapio, Jonna Bister, Niklas K. Bjoerkstroem, Hans-Gustaf Ljunggren
Summary: Natural killer cells play an important role in the immune response to SARS-CoV-2 infection, with early activation and changes in numbers observed. They exhibit antiviral activity against SARS-CoV-2 through direct cytotoxicity and cytokine secretion. Hyperactivation and misdirected responses of natural killer cells may contribute to the pathogenesis of COVID-19.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)