Article
Cell Biology
Yanlin Cai, Yi Hu, Furong Yu, Wenjuan Tong, Shufen Wang, Shunliang Sheng, Jiayu Zhu
Summary: Globally, ovarian cancer ranks as the second most common cause of gynecologic-related cancer deaths among women, with a poor prognosis. Research has shown that downregulation of AHNAK in ovarian cancer cells can significantly inhibit cell growth and metastasis, partly by dampening the Canonical Wnt cascade. Therefore, AHNAK may serve as a biomarker and potential treatment target for ovarian cancer.
Article
Medicine, Research & Experimental
Wei Zhang, Xianhui Ruan, Yaoshuang Li, Jingtai Zhi, Linfei Hu, Xiukun Hou, Xianle Shi, Xin Wang, Jinpeng Wang, Weike Ma, Pengfei Gu, Xiangqian Zheng, Ming Gao
Summary: The study reveals that KDM1A regulates the stemness of thyroid cancer and promotes cancer progression through the Wnt/beta-catenin pathway. Inhibiting KDM1A can significantly inhibit thyroid cancer progression and enhance sensitivity to chemotherapy.
Article
Oncology
Senwen Feng, Junhao Liu, Hailiang Li, Jianfan Wen, Yujun Zhao, Xiaofeng Li, Guankun Lu, Peng Gao, Xiancheng Zeng
Summary: RAD54B is significantly upregulated in HCC and may serve as a potential prognostic marker and therapeutic target; RAD54B promotes HCC progression mainly through modulating the Wnt/β-catenin signaling pathway; Inhibition of the Wnt/β-catenin signaling pathway can counteract the activating effects of RAD54B on the motility of HCC cells.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Neurosciences
Zong-qing Zhao, Xiu-jie Wu, Yan-hao Cheng, Yun-fei Zhou, Xi-meng Ma, Jian Zhang, Xue-yuan Heng, Fan Feng
Summary: The study demonstrated that TROAP plays an oncogenic role in glioma by activating the Wnt/beta-Catenin signaling pathway, leading to cell proliferation, migration, invasion, and poor prognosis. Inhibition of TROAP or downstream targets of Wnt/beta-Catenin pathway could reverse the tumor-promoting effects, suggesting TROAP as a potential novel target for glioma therapy.
CNS NEUROSCIENCE & THERAPEUTICS
(2021)
Article
Reproductive Biology
Wenjing Hu, Min Li, Youguo Chen, Xinxian Gu
Summary: The research revealed that high expression of UBE2S in HGSOC is associated with poor prognosis. UBE2S promotes proliferation, migration, and invasion of ovarian cancer, and increases Olaparib resistance by activating the Wnt/β-catenin signaling pathway.
JOURNAL OF OVARIAN RESEARCH
(2021)
Article
Anatomy & Morphology
Ruofei He, Yu Chen, Chenyu Qian, YiLin Hu, Xinkun Huang, Ran Tao
Summary: This study investigated the role and mechanism of DVL in gastric cancer development, and found that DVL2 is highly expressed in gastric cancer tissues, which is significantly correlated with clinicopathological parameters and patient survival. Inhibiting DVL2 can suppress gastric cancer cell proliferation, migration, and invasion, suggesting that DVL2 may be a new therapeutic target for gastric cancer.
Article
Oncology
Yanqiu Bao, Jingshu Cui, Yuyang Yue, Shuxia Cao, Xiangdan Li, Lan Liu
Summary: Ebp1 is highly expressed in malignant melanoma and is associated with clinical stage and lymph node metastases. Knockdown of Ebp1 inhibits cell proliferation, migration, and invasion in melanoma. Ebp1 may promote the proliferation and metastasis of melanoma cells through activation of the Wnt/β-catenin pathway.
CANCER CELL INTERNATIONAL
(2022)
Article
Oncology
Zhiyong Li, Yunning Huang, Yuanyi Xu, Xiaofei Wang, Honghong Wang, Shuai Zhao, Han Liu, Guangfu Yu, Xiangming Che
Summary: This study identified a novel oncogenic function of ADAR1 in peritoneal metastasis of gastric cancer through the Wnt/beta-catenin pathway. ADAR1 may serve as a potential marker and therapeutic target against gastric cancer metastasis by regulating key pathways in cancer progression.
Article
Cell & Tissue Engineering
Hao Yu, Yan Liu, Xiangwen Yang, Jiajing He, Fan Zhang, Qun Zhong, Xiaojing Guo
Summary: Strontium ranelate can promote BMSCs chondrogenic differentiation by inhibiting the Wnt/beta-catenin signaling pathway and accelerate cartilage regeneration in rat femoral condyle defects.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Jian-Hua Chang, Bo-Wen Xu, Di Shen, Wei Zhao, Yue Wang, Jia-liang Liu, Guang-Xiao Meng, Guang-Zhen Li, Zong-Li Zhang
Summary: In this study, BRF2 was identified and validated as an oncogene in hepatocellular carcinoma (HCC), shedding light on the pathogenesis and therapeutic possibilities for HCC. BRF2 expression was upregulated in HCC, while its depletion inhibited HCC metastasis and invasion. Additionally, miR-409-3p was identified as a regulator of BRF2, and the Wnt/beta-catenin signaling pathway may play a role in early HCC development.
CANCER CELL INTERNATIONAL
(2023)
Article
Plant Sciences
Yuhui Mi, Lei Zhong, Saijian Lu, Po Hu, Yang Pan, Xuelin Ma, Binghui Yan, Zhenhuan Wei, Guangming Yang
Summary: The study aimed to evaluate the healing effect of quercetin on cutaneous wound models in vivo and in vitro, and to reveal the mechanisms involved, including the Wnt/beta-catenin pathway and TERT.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Feilong Hong, Yanjiao Mao, Xinwen Huang
Summary: UNC119 plays a role in the tumorigenesis of nasopharyngeal carcinoma by promoting cell proliferation, invasion, and migration, as well as epithelial-mesenchymal transition. Knockdown of UNC119 suppresses cell growth and metastasis in nasopharyngeal carcinoma by inhibiting the activation of the Wnt/β-catenin pathway.
Article
Cell Biology
Zimin Xiang, Shuai Zhang, Xiaodong Yao, Libin Xu, Jianwei Hu, Chenghui Yin, Jianmei Chen, Hao Xu
Summary: Resveratrol can promote function recovery and axonal regeneration, improve histological damage, and inhibit apoptosis level after SCI by regulating the Wnt/beta-catenin signaling pathway. This study expanded the regulatory mechanism of resveratrol in SCI injury.
Article
Oncology
Dan Fang, Mu-Ru Wang, Jia-Lun Guan, Ying-Ying Han, Jia-Qi Sheng, De-An Tian, Pei-Yuan Li
Summary: The study found that in HCC, high expression of BRD9 was closely related to malignancy, tumor stage, tumor size, and prognosis. BRD9 promoted cell proliferation and tumor growth, and mediated its effects by activating the Wnt/beta-catenin signaling pathway.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Anatomy & Morphology
Chunyi Ji, Lijian Chen, Miaoxian Yuan, Weixin Xie, Xinyi Sheng, Qiang Yin
Summary: This study investigates the mechanism of KDM1A-regulated hepatoblastoma (HB) development. The expression levels of KDM1A and DKK3 were analyzed in HB patients, and experiments were conducted on cell lines and nude mice to study the effects of knockdown of KDM1A and DKK3. The results suggest that KDM1A stimulates HB development by activating the Wnt/beta-catenin pathway through inhibition of DKK3 transcription.
Article
Oncology
Yuxiang Lin, Ling Lin, Fangmeng Fu, Chuan Wang, Anqi Hu, Juanjuan Xie, Meichen Jiang, Zhenxin Wang, Lujie Yang, Rongrong Guo, Pengyuan Yang, Huali Shen
Summary: The study utilized high-resolution mass spectrometry to explore the proteomic profiles of TNBC patients, revealing significant differences in protein expression levels between different pathological subgroups. TNBC cancer tissues showed activation of various signaling pathways and metabolic processes, providing potential therapeutic targets for accurate classification and treatment.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Biochemical Research Methods
Yudong Guan, Min Zhang, Manasi Gaikwad, Hannah Voss, Ramin Fazel, Samira Ansari, Huali Shen, Jigang Wang, Hartmut Schlueter
Summary: This study presents an in-depth analytical strategy for glycosylation of therapeutic glycoproteins, including N-glycan analysis, site-specific N-glycosylation analysis, and O-glycan identification. By using mass spectrometry and Python scripts, the study identified N-glycan compositions, revealed site-specific N-glycosylation, and analyzed different glycan modifications such as phosphorylation and O-acetylation. The integrated strategy proved to be effective in understanding the pharmacological properties of therapeutic glycoproteins and improving manufacturing processes.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Weimin Zheng, Yang Zhang, Chuanyu Sun, Shengyang Ge, Yifan Tan, Huali Shen, Pengyuan Yang
Summary: The study systematically and comprehensively investigated the gene and protein expression between the testis and epididymis, revealing their crucial roles in male fertility. The results help scientists have a deeper understanding of the physiological functions of the testis and epididymis, especially with important discoveries on the expression patterns of missing proteins.
Article
Immunology
Lujie Yang, Tianqi Gong, Huali Shen, Jiangnan Pei, Lei Zhang, Quanqing Zhang, Yuanyu Huang, Zuojian Hu, Ziyue Pan, Pengyuan Yang, Ling Lin, Hongxiu Yu
Summary: Macrophages are crucial immune cells involved in immune responses, and their activation by different pathogens leads to significant changes in glycoproteins, affecting receptors like Tlr2 and CD14 in the Toll-like receptor pathway. The study highlights the dynamic role of glycosylation in macrophage functions under various stimuli.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Zhenxin Wang, Quanqing Zhang, Huali Shen, Pengyuan Yang, Xinwen Zhou
Summary: In the study of polymer analysis based on MALDI-TOF MS, characteristic profiling of four representative polymers and optimization of sample preparation methods were conducted. A universal matrix for all four polymers was identified, along with preferences of different polymers for cationization reagents and solvents. The optimized method was successfully applied to the identification of different polymerized degrees, suggesting potential supplementary functions in biomedical and visible probing applications.
FRONTIERS IN CHEMISTRY
(2021)
Article
Cardiac & Cardiovascular Systems
Qing-Jun Zhang, Yu He, Yongnan Li, Huali Shen, Ling Lin, Min Zhu, Zhaoning Wang, Xiang Luo, Joseph A. Hill, Dian Cao, Richard L. Luo, Raymond Zou, John McAnally, Jun Liao, Pietro Bajona, Qun S. Zang, Yonghao Yu, Zhi-Ping Liu
Summary: The study revealed a pathological role of Cilp1 in promoting post-myocardial infarction cardiac remodeling and identified a novel function of Cilp1 in promoting myofibroblast proliferation. Cilp1 may serve as a potential biomarker for pathological cardiac remodeling and a target for fibrotic heart disease.
CIRCULATION RESEARCH
(2021)
Article
Medicine, Research & Experimental
Yueting Xiong, Yongtao Zheng, Yan Yan, Jun Yao, Hebin Liu, Fenglin Shen, Siyuan Kong, Shuang Yang, Guoquan Yan, Huanhuan Zhao, Xinwen Zhou, Jia Hu, Bin Zhou, Tao Jin, Huali Shen, Bing Leng, Pengyuan Yang, Xiaohui Liu
Summary: This study aimed to discover specific, sensitive, and non-invasive biomarkers for the diagnosis and classification of ruptured and unruptured intracranial aneurysm (IA), to benefit the development of treatment strategies. By using a deep learning-based method, a total of 113 potential markers were identified, and two sets of biomarker combinations were built for accurate classification.
EMBO MOLECULAR MEDICINE
(2022)
Article
Electrochemistry
Yingchao Liu, Yuanyu Huang, Mengxi Wu, Siyuan Kong, Weiqian Cao, Shunxiang Li, Guoquan Yan, Baohong Liu, Pengyuan Yang, Quanqing Zhang, Liang Qiao, Huali Shen
Summary: In this study, a novel microfluidic free-flow paper electrochromatography (mu-FFPE) method was developed by combining mu-FFE with filter paper-based chromatography, enabling continuous separation of charged analytes. The addition of filter paper as the stationary phase improved the stability of the separation system. The mu-FFPE showed great potential in the separation and analysis of various biological molecules.
Article
Physics, Atomic, Molecular & Chemical
Shunxiang Li, Anqi Hu, Yingchao Liu, Xiaohui Liu, Kai Liu, Chenxin Zhu, Hongxiu Yu, Weiqian Cao, Huali Shen, Quanqing Zhang, Pengyuan Yang
Summary: The efficient ion transmission and fragmentation ability of the novel electrodynamic ion transmission device (MIR F-Disc) make it suitable for mini-mass spectrometry, improving sensitivity and dynamic range in mass spectrometry.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Cell Biology
Na Li, Beilei Hua, Qing Chen, Fukang Teng, Meiyu Ruan, Mengnan Zhu, Li Zhang, Yinbo Huo, Hongqin Liu, Min Zhuang, Huali Shen, Huanhu Zhu
Summary: The mTOR-dependent nutrient-sensing and response machinery is essential for animals to regulate cellular and developmental programs. However, the specific nutrient and metabolite signals involved in mTOR regulation and their effects at the organism level are not well understood. In this study, the researchers identified the critical role of intestinal peroxisomes in antagonizing the GlcCer-mTORC1-mediated nutrient signal. They found that GlcCer deficiency, inactive mTORC1, or prolonged starvation caused the relocation of intestinal peroxisomes closer to the apical region, leading to the release of glycolipid hormones that affect animal development. These findings highlight the importance of the gut-brain axis in nutrient-sensing-dependent development in animals.
Correction
Biochemical Research Methods
Yudong Guan, Min Zhang, Manasi Gaikwad, Hannah Voss, Ramin Fazel, Samira Ansari, Huali Shen, Jigang Wang, Hartmut Schluter
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Analytical
Anqi Hu, Lei Zhang, Zhenxin Wang, Chunyan Yuan, Ling Lin, Jiayi Zhang, Xia Gao, Xuguang Chen, Wei Guo, Pengyuan Yang, Huali Shen
Summary: A high-sensitivity, high-throughput, and precise pan-targeted serum proteomic strategy was developed for efficient cancer serum proteomic research and biomarker discovery. The strategy utilized the Cancer Serum Atlas to acquire standard MS assays and spectra of cancer-secreted proteins and employed the peptide-anchored parallel reaction monitoring (SPA-PRM) method for precise quantification. With this strategy, 325 cancer-related serum proteins were quantified in 288 serums, leading to the discovery of potential biomarkers for early cancer detection. A proteomic-based multicancer detection model was built, showing high sensitivity, specificity, and localization accuracy. The Cancer Serum Atlas provides a wide range of potential biomarkers and the pan-targeted proteomic strategy enables efficient biomarker discovery and multicancer detection.
ANALYTICAL CHEMISTRY
(2023)
Article
Psychology, Multidisciplinary
Xiao Pan, Xiaokang Zhao, Huali Shen
Summary: With the advancement of mobile communication technology and changing work methods, work interruptions have become a common challenge for employees. However, there is a lack of research on human work interruptions in China, particularly in comparison to virtual work interruptions. This study conducted in-depth interviews with 29 employees and developed a psychological and behavioral model for employees facing human work interruptions. The model highlights the role of cognitive appraisals, affective responses, and behavioral changes, and identifies the influence of personal traits and work environment on these responses. The findings contribute to the interruption theory and provide implications for human resource management practices.
FRONTIERS IN PSYCHOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Jiayi Zhang, Anqi Hu, Xuguang Chen, Fenglin Shen, Lei Zhang, Yuxiang Lin, Huali Shen
Summary: Serum/plasma proteome analysis is important for monitoring physiological and pathological changes in the human population. A established Common Serum Protein Database has been integrated with the Cancer Serum Atlas to create the comprehensive cancer serum protein atlas (DCCSP Atlas). This atlas enables the targeting of common serum proteins and cancer-secreted proteins, improving the sensitivity and specificity of multi-cancer detection and classification.
Article
Nanoscience & Nanotechnology
Ling Lin, Shanshan Liu, Zhangwei Chen, Yan Xia, Juanjuan Xie, Mingqiang Fu, Danbo Lu, Yuan Wu, Huali Shen, Pengyuan Yang, Juying Qian
Summary: Dilated cardiomyopathy (DCM) is characterized by left ventricular dilatation and contractile dysfunction, and its molecular mechanisms are not fully understood. This study examined the transcriptome and proteome of healthy and DCM myocardial tissue, identifying dysregulated processes such as extracellular matrix, mitochondrial function, and muscle contraction. Furthermore, a 4-biomarker panel (CTSB, vWF, C9, and MFGE8) was developed for the diagnosis of DCM.