Article
Biochemistry & Molecular Biology
Ji Young Kim, Eun Jung Lee, Yuri Ahn, Sujin Park, Yu Jeong Bae, Tae Gyun Kim, Sang Ho Oh
Summary: The study demonstrates that CTSL is a novel target gene of HIF-1 alpha and plays a role in melanosome degradation in melanocytes through lysosomal activity during autophagosome-lysosome fusion. Under autophagy-inducing starvation conditions, the expression of HIF-1 alpha and CTSL is upregulated in melan-a cells. Inhibition of conversion of pro-CTSL to mature CTSL leads to accumulation of certain proteins due to decreased lysosomal activity in the autophagic pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Flavia Danniele Frota Machado, Rodrigo de Oliveira Formiga, Gedson Rodrigues de Morais Lima, Neyres Zinia Taveira de Jesus, Edvaldo Balbino Alves Junior, Alexsandro Fernandes Marinho, Josean Fechine Tavares, Flavia Almeida Santos, Ana Flavia Seraine Custodio Viana, Aurigena Antunes Araujo, Raimundo Fernandes de Araujo Junior, Claudia Helena Pellizzon, Leonia Maria Batista
Summary: The extract of Hyptis suaveolens exhibits anti-inflammatory activity in the intestine by reducing inflammation, cellular damage, and oxidative stress, as well as regulating cell proliferation.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Thuy-Hang Nguyen, Stephanie Conotte, Alexandra Belayew, Anne-Emilie Decleves, Alexandre Legrand, Alexandra Tassin
Summary: Muscular dystrophies are genetic degenerative muscle disorders characterized by muscle wasting, often accompanied by respiratory impairments and hypoxemia. Hypoxic stress activates compensatory mechanisms, with HIF-1α playing a key role. Alterations in HIF-1α in muscle may impact the pathophysiology of muscular dystrophies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ba Da Yun, Seung Wan Son, Soo Young Choi, Hyo Jeong Kuh, Tae-Jin Oh, Jong Kook Park
Summary: HIF-1α plays a critical role in cancer, with its regulation affecting the malignant characteristics of cancer. Plant-derived phytochemicals have shown potential in inhibiting HIF-1α and combating various types of cancer. A deeper understanding of phytochemicals is crucial for the development of advanced cancer treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Jinsheng Zhu, Li Zhao, Yadan Hu, Guoqi Cui, Ang Luo, Changlei Bao, Ying Han, Tong Zhou, Wenju Lu, Jian Wang, Stephen M. Black, Haiyang Tang
Summary: This study elucidates the importance of HIF2 alpha in the pathogenesis of pulmonary arterial hypertension, demonstrating the potential of HIF2 alpha-mediated gene sets as a diagnostic tool for distinguishing PAH patients. The study also found significant differences in the expression profiles of HIF2 alpha-mediated gene sets in lung tissues and peripheral blood mononuclear cells between control subjects and PAH patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Tripti Singh, Pallabi Banerjee, Uditi, Sarita Kumari, Anita Chopra, Nagendra Singh, Imteyaz Qamar
Summary: Regucalcin regulates intracellular Ca2+ homeostasis and intracellular signaling pathways. Its expression in hypoxia is upregulated by HIF-1α through direct binding to the HRE binding motifs within the Regucalcin promoter.
Review
Cell Biology
Mykyta Malkov, Chee Teik Lee, Cormac T. Taylor
Summary: Hypoxia and inflammation frequently co-occur in inflammatory diseases, with pro-inflammatory cytokines such as TNF-alpha and IL-1 beta directly impacting HIF signaling pathways. Multi-level crosstalk between inflammatory and hypoxic signaling pathways plays a crucial role in shaping the degree of inflammation at hypoxic sites.
Review
Oncology
Kenneth N. Huynh, Sriram Rao, Bradley Roth, Theodore Bryan, Dayantha M. Fernando, Farshid Dayyani, David Imagawa, Nadine Abi-Jaoudeh
Summary: Hypoxia-inducible factor 1 alpha (HIF-1a) is a transcription factor that regulates the cellular response to hypoxia and is upregulated in all types of solid tumor. Hepatocellular carcinoma (HCC) is a highly vascular and hypoxic tumor, and treatments like TACE and TAE can exacerbate hypoxia in HCC. Hypoxia-activated prodrugs (HAPs) have potential as a targeted treatment for hypoxic HCC, but more research is needed to understand their effects in combination with embolization.
Article
Biochemistry & Molecular Biology
Bei Zhang, Lutong Wang, Lin Wang, Yihai Wang, Jingwen Xu, Xiangjiu He
Summary: Twenty-six eudesmanolides, including six undescribed compounds, were isolated from the flowers of Sphagneticola trilobata and their structures were determined using spectroscopic techniques, NMR calculation, and DP4+ analysis. The crystal structure of compound (1) was confirmed using X-ray diffraction. Various eudesmanolides exhibited cytotoxic and anti-inflammatory effects in different assays. Compounds (3) and (8) showed significant cytotoxic effects against AGS cell line, while compounds (2) and (7) exhibited inhibitory effects on nitric oxide production and anti-inflammatory effects in RAW 264.7 macrophages. These findings suggest the potential of eudesmanolides from S. trilobata for further research as lead compounds.
Article
Biochemistry & Molecular Biology
Kenta Kaihara, Shuji Nakagawa, Yuji Arai, Hiroaki Inoue, Shinji Tsuchida, Yuta Fujii, Yoichiro Kamada, Tsunao Kishida, Osam Mazda, Kenji Takahashi
Summary: The study found that hypoxia can suppress the development of rheumatoid arthritis by controlling HIF-1 alpha expression, delaying joint swelling and reducing joint destruction. Deferoxamine-mediated activation of HIF-1 alpha can counteract the inhibitory effect of sustained hypoxia on pro-inflammatory cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Yiwei Liu, Qipeng Luo, Zhanhao Su, Junyue Xing, Jinlin Wu, Li Xiang, Yuan Huang, Haizhou Pan, Xie Wu, Xiaoling Zhang, Jun Li, Fuxia Yan, Hao Zhang
Summary: Cyanotic congenital heart disease (CCHD) leads to maladaptation of cardiac metabolism during puberty, accompanied by impaired cardiac function. The study identified HIF-1 alpha as the key regulator of cardiac metabolic adaptation in CH animal models, with pubertal insulin resistance potentially suppressing its expression.
Article
Gastroenterology & Hepatology
Lin Zhou, Yang Zhao, Li-Chao Pan, Jing Wang, Xian-Jie Shi, Guo-Sheng Du, Qiang He
Summary: This study found that HIF-1 alpha plays a role in promoting tumor proliferation and invasion in liver cancer. Inhibiting the PI3K/Akt/mTOR-HIF-1 alpha and HIF-1 alpha-PTEN signaling pathways can enhance the anti-cancer effect of Huai Er.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Review
Immunology
Qianyan He, Yinzhong Ma, Jie Liu, Dianhui Zhang, Jiaxin Ren, Ruoyu Zhao, JunLei Chang, Zhen-Ni Guo, Yi Yang
Summary: Ischemic stroke, caused by inadequate cerebrovascular blood and oxygen supply, poses a significant global health burden. HIF-1α, a key regulator of oxygen homeostasis, plays a critical role in various aspects of stroke pathophysiology and is an important target for potential therapeutic interventions. Further research into the differential regulation of HIF-1α in different cell types during ischemic stroke is crucial for understanding the disease mechanism and identifying novel treatment strategies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Luise A. Roser, Pelin Erkoc, Rebecca Ingelfinger, Marina Henke, Thomas Ulshoefer, Ann-Kathrin Schneider, Volker Laux, Gerd Geisslinger, Imke Schmitt, Robert First, Susanne Schiffmann
Summary: Lichen extracts containing compounds such as lecanoric acid possess anti-proliferative effects, particularly in cancer cells. Lecanoric acid exerts its anti-proliferative effects by arresting cells in the M phase. Importantly, it does not affect primary immune cells. These findings suggest that lecanoric acid may be a potential candidate for anti-cancer therapy.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Je Lin Sieow, Hweixian Leong Penny, Sin Yee Gun, Ling Qiao Tan, Kaibo Duan, Joe Poh Sheng Yeong, Angela Pang, Diana Lim, Han Chong Toh, Tony Kiat Hon Lim, Edgar Engleman, Olaf Rotzschke, Lai Guan Ng, Jinmao Chen, Suet Mien Tan, Siew Cheng Wong
Summary: A large number of neutrophils infiltrate tumors and contribute to the inflammatory tumor microenvironment. The metabolic status of these neutrophils and their role in tumor progression were investigated in a mouse model of pancreatic ductal adenocarcinoma (PDAC). Tumor-infiltrating neutrophils showed enhanced glycolytic activity compared to neutrophils from the bone marrow and blood of the same mouse. Deletion of hypoxia-inducible factor 1-alpha (HIF-1 alpha) in neutrophils reduced tumor burden and improved survival in the mouse model, indicating a potential target for metabolic modulation in cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Nicola Borbone, Ilaria Piccialli, Andrea Patrizia Falanga, Vincenzo Piccialli, Giovanni N. Roviello, Giorgia Oliviero
Summary: The recent development of mRNA vaccines against SARS-CoV-2 infection has brought attention to the potential of nucleic acids as innovative prophylactic agents and diagnostic and therapeutic tools. The use of nucleic acids has been limited by their reduced half-life and difficulties in transport, which can now be overcome through chemical modifications and appropriate nanocarriers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Francesca Greco, Domenica Musumeci, Nicola Borbone, Andrea Patrizia Falanga, Monica Terracciano, Ilaria Piccialli, Giovanni Nicola Roviello, Giorgia Oliviero
Summary: This study explored the binding properties of trans-polydatin with DNA G-quadruplex structure using CD spectroscopy and molecular docking simulations, and found that trans-polydatin can interact with G-quadruplex structure.
Article
Biochemistry & Molecular Biology
Francesca Greco, Andrea Patrizia Falanga, Monica Terracciano, Carlotta D'Ambrosio, Gennaro Piccialli, Giorgia Oliviero, Giovanni Nicola Roviello, Nicola Borbone
Summary: 1,3-bis(1'-uracilyl)-2-propanone can bind serum albumin and induce partial protein destabilization by altering its secondary structure.
News Item
Biochemistry & Molecular Biology
Massimiliano Mazzone, Alessandra Castegna
Summary: Studies on the microbiome-host interaction reveal metabolic mutual crosstalk between host tissues and gut microbiota.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Oncology
Charlotte Bisilliat Donnet, Valerie Acolty, Abdulkader Azouz, Anaelle Taquin, Coralie Henin, Sarah Trusso Cafarello, Sebastien Denanglaire, Massimiliano Mazzone, Guillaume Oldenhove, Oberdan Leo, Stanislas Goriely, Muriel Moser
Summary: The PHD/HIF pathway is involved in immune and inflammatory processes, including in the hypoxic tumor microenvironment. Deleting PHD2 in T lymphocytes resulted in regression of EG7-OVA tumors, which depended on HIF-1a. The enhanced control of tumor growth was associated with increased polyfunctionality of CD8+ tumor-infiltrating lymphocytes, indicating the importance of glycolysis in sustaining CTL activity.
CANCER IMMUNOLOGY RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Andrea P. Falanga, Monica Terracciano, Giorgia Oliviero, Giovanni N. Roviello, Nicola Borbone
Summary: G-quadruplex (G4) structures in plants have significant implications in biological processes and pathological states. The exploration of plant compounds and G4 ligands can lead to potential anticancer treatments and biomedical applications.
Article
Biochemistry & Molecular Biology
Monica Terracciano, Simas Rackauskas, Andrea Patrizia Falanga, Sara Martino, Giovanna Chianese, Francesca Greco, Gennaro Piccialli, Guido Viscardi, Luca De Stefano, Giorgia Oliviero, Nicola Borbone, Ilaria Rea
Summary: The physicochemical properties of three different ZnO tetrapodal nanostructures (ZnO-Ts) synthesized by a combustion process were studied and evaluated for label-free biosensing purposes. The chemical reactivity of ZnO-Ts was explored by quantifying the available functional hydroxyl groups (-OH) on the transducer surface necessary for biosensor development. The best ZnO-T sample was chemically modified and bioconjugated with biotin as a model bioprobe, confirming their suitability for biosensing applications through streptavidin target detection experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Domenica Capasso, Paola Marino, Sonia Di Gaetano, Nicola Borbone, Monica Terracciano, Roberta Trani, Caterina Longo, Vincenzo Piccialli
Summary: Several five-membered bromolactones were synthesized from D-xylonolactone and D-ribonolactone, which are related to the C1-C5 portion of mycalin A lactone. The bromination of D-ribonolactone with HBr/AcOH, without transesterification, resulted in the majority of the acetylated lactones investigated. The evaluation of their anti-tumor activity indicated that the acetates possess cytotoxicity towards multiple cancer cells, with selectivity towards tumor cells and limited toxicity on healthy cells.
Article
Polymer Science
Sabrina Giordano, Enrico Gallo, Carlo Diaferia, Elisabetta Rosa, Barbara Carrese, Nicola Borbone, Pasqualina Liana Scognamiglio, Monica Franzese, Giorgia Oliviero, Antonella Accardo
Summary: Multicomponent hydrogels based on ultrashort aromatic peptides have been widely studied for their applications in tissue engineering, drug delivery, diagnostics, and biosensing. The incorporation of additional molecules into these hydrogels allows for the development of novel hydrogels with improved mechanical and functional properties. These hybrid hydrogels are biocompatible and support cell adhesion, proliferation, and differentiation, making them potential scaffolds for cell culture.
Article
Polymer Science
Maria Marzano, Nicola Borbone, Felice Amato, Giorgia Oliviero, Pierpaolo Fucile, Teresa Russo, Filomena Sannino
Summary: Three-dimensional chitosan-gallic acid complexes were prepared by adsorbing gallic acid on three-dimensional chitosan structures. The complexes exhibited high antioxidant activity and antimicrobial capacity, making them suitable for nutraceutical, cosmeceutical, and pharmaceutical applications.